Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Plant Regeneration from Seed Derived Callus of four Cultivars of Timothy

티모시의 품종에 따른 성숙종자 유래의 캘러스로부터 식물체 재분화

  • Lee, Ki-Won (National Institute of Animal Science, RDA) ;
  • Choi, Gi-Jun (National Institute of Animal Science, RDA) ;
  • Kim, Ki-Yong (National Institute of Animal Science, RDA) ;
  • Ji, Hee-Chung (National Institute of Animal Science, RDA) ;
  • Jung, Min-Wong (National Institute of Animal Science, RDA) ;
  • Kim, Kyung-Hee (Division of Applied Life Science (BK21 program), Gyeongsang National University) ;
  • Lee, Byung-Hyun (Division of Applied Life Science (BK21 program), Gyeongsang National University) ;
  • Lee, Sang-Hoon (National Institute of Animal Science, RDA)
  • 이기원 (농촌진흥청 국립축산과학원) ;
  • 최기준 (농촌진흥청 국립축산과학원) ;
  • 김기용 (농촌진흥청 국립축산과학원) ;
  • 지희정 (농촌진흥청 국립축산과학원) ;
  • 정민웅 (농촌진흥청 국립축산과학원) ;
  • 김경희 (경상대학교 응용생명과학부) ;
  • 이병현 (경상대학교 응용생명과학부) ;
  • 이상훈 (농촌진흥청 국립축산과학원)
  • Received : 2011.04.29
  • Accepted : 2011.07.05
  • Published : 2011.09.30
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Abstract

The present study was conducted to determine the optimum in vitro conditions for callus induction and plant regeneration from mature seed derived callus of four cultivars of Timothy. In order to investigate the effects of genetic variations of timothy in tissue culture, calli were induced from mature seeds of four varieties, 'Colt', 'Chair', 'Richmond' and 'Hokuo' and plant regeneration frequency was compared. Significant differences were observed among the cultivars in both callus induction and plant regeneration. Genotype 'Colt' consistently performed best in the callus subculture and plant regeneration. The complete plantlets were thereafter transplanted to grow under greenhouse condition. Regenerated timothy plants were morphologically uniform with normal leaf and growth pattern.

티모시의 4가지 품종을 이용하여 성숙종자로 부터 캘러스 유도 및 식물체 재분화 효율을 조사하였다. 4주 동안 배양한 종자로부터 캘러스 유도율은 Colt, Chair, Richmond, Hokuo 순으로 캘러스 유도율을 보였고, 식물체로의 재분화 또한 캘러스 유도율과 같은 Colt > Chair > Richmond > Hokuo 순으로 나타났다. 기본 배지의 종류에 따른 배양 효율의 차이는 품종별 차이는 있으나 MS 배지가, 식물체 재분화는 N6 배지와 MS 배지가 SH 배지에 비해 효과적이었다. 전체적으로는 캘러스 유도율이 높은 품종이 식물체 재분화율도 높은 경향을 나타내었다. 본 연구를 통하여 밝혀진 캘러스 유도와 식물체 재분화율이 우수한 Colt 품종은 유용유전자 도입을 통한 신품종 개발에 유용하게 이용될 수 있을 것이다.

Keywords

References

  1. 김기용, 지희정, 이상훈, 이기원, 김원호, 정민웅, 서 성, 최기준. 2010. 이탈리안 라이그라스 춘파 재배에서 조.만성 품종간 생산성 비교. 초지조사료지. 30(2):97-102.
  2. 이효원, 김동암. 2003. 초지학. 한국방송통신대학교출판부. pp. 117-119.
  3. 최기준, 임영철, 지희정, 김기용, 박형수, 서 성, 문정섭, 김대호, 이상훈. 2010. 내재해 다수성 톨페스큐 신품종 "그린마스터"의 품종 특성. 한국초지조사료지 30(3):199-204.
  4. Chen, L., C. Auh, P. Dowling, J. Bell, F. Chen, A. Hopkins, R. A. Dixon and Z.-Y. Wang. 2003. Improved forage digestibility of tall fescue (Festuca arundinacea) by transgenic down-regulation of cinnamyl alcohol dehydrogenase. Plant Biotechnol J. 1:437-449. https://doi.org/10.1046/j.1467-7652.2003.00040.x
  5. Chen, L., C. Auh, P. Dowling, J. Bell, D. Lehmann and Z.-Y. Wang. 2004. Transgenic down-regulation of caffeic acid O-methyltransferase (COMT) led to improved digestibility in tall fescue (Festuca arundinacea). Funct Plant Biol. 31:235-245. https://doi.org/10.1071/FP03254
  6. Chu, C.C., C.S. Wang, C.C. Sun, C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic. 18:659-668.
  7. Forster J.W. and G. Spangenberg. 1999. Forage and turf grass biotechnology: principles, methods and prospects. In: Setlow JK (eds), Genetic engineering: principles and methods, Vol 21, Kluwer Academic Publishers, New York, pp. 191-237
  8. Guo, Y.D. and S. Pulli. 2001. Somatic embryogenesis and plant regeneration from suspension cultures of timothy (Phleum pratense L.). Act Agr Scand B-S P. 51:160-166. https://doi.org/10.1080/09064710127621
  9. Lee, K.-W., S.-H. Lee, D.-G. Lee, D.-H. Kim and B.-H. Lee. 2006. Effect of orchardgrass varieties on callus culture and palnt regeneration. J. korean Grassl. Sci. 26:191-198.
  10. Lee, K.-W., K.-Y. Kim, G.J. Choi, Y.C. Lim, W.H. Kim, M.W. Jung, S. Seo, B.-H. Lee and S.-H. Lee. 2008. Callus Induction and plant regeneration from mature seeds of timothy. J. Kor. Grassl. Forage Sci. 28:165-170. https://doi.org/10.5333/KGFS.2008.28.3.165
  11. Lee, K.-W., J.K. Lee, K.-Y. Kim, H.C. Ji, H.S. Park, K.-H. Kim, B.-H. Lee and S.-H. Lee. 2009a. Plant regeneration capacity of calluses derived from mature seed of perennial ryegrass cultivars. J. Kor. Grassl. Forage Sci. 29:285-290. https://doi.org/10.5333/KGFS.2009.29.4.285
  12. Lee, K.-W., G.J. Choi, K.-Y. Kim, H.C. Ji, H.S. Park, S.H. Yoon. and S.-H. Lee. 2009b. High frequency plant regeneration from mature seed derived callus of Italian ryegrass (Lolium multiflorum) cultivars. African Journal of Biotechnology. 8 (24), pp. 6828-6833.
  13. Lee, S.-H., N. Ahsan, K.-W. Lee, D.-H. Kim, D.-G. Lee, S.S. Kwak, S.-Y. Kwon, T.H. Kim and B.-H. Lee. 2007. Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. J Plant Physiol. 164:1626-1638. https://doi.org/10.1016/j.jplph.2007.01.003
  14. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Plazek, A., M. Filek and M. Wfdzony. 1999. Improvement of regeneration ability in Phleum pratense L. in vitro culture by dicamba. Acta Physiologiae Plantarum. 21:397-403. https://doi.org/10.1007/s11738-999-0012-1
  16. Schenk, R. U and A. C. Hildebrandt. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199-204. https://doi.org/10.1139/b72-026
  17. Somers, D. A., D. A. Samac and P. M. Olhoft. 2003. Recent advances in legume transformation. Plant Physiol. 131:892-899. https://doi.org/10.1104/pp.102.017681
  18. Spangenberg, G., Z. Y. Wang and I. Potrykus. 1998. Biotechnology in forage and turf grass improvement. In: Frankel et al. (Eds), Monographs on theoretical and applied genetics, Vol. 23, Springer Verlag, Heidelberg, p. 192.
  19. Wang, Z.-Y., X.D. Ye, J. Nagel, I. Potrykus and G. Spangenberg. 2001. Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants. Plant Cell Rep. 20:213-219. https://doi.org/10.1007/s002990000299