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http://dx.doi.org/10.5333/KGFS.2011.31.3.211

Plant Regeneration from Seed Derived Callus of four Cultivars of Timothy  

Lee, Ki-Won (National Institute of Animal Science, RDA)
Choi, Gi-Jun (National Institute of Animal Science, RDA)
Kim, Ki-Yong (National Institute of Animal Science, RDA)
Ji, Hee-Chung (National Institute of Animal Science, RDA)
Jung, Min-Wong (National Institute of Animal Science, RDA)
Kim, Kyung-Hee (Division of Applied Life Science (BK21 program), Gyeongsang National University)
Lee, Byung-Hyun (Division of Applied Life Science (BK21 program), Gyeongsang National University)
Lee, Sang-Hoon (National Institute of Animal Science, RDA)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.31, no.3, 2011 , pp. 211-216 More about this Journal
Abstract
The present study was conducted to determine the optimum in vitro conditions for callus induction and plant regeneration from mature seed derived callus of four cultivars of Timothy. In order to investigate the effects of genetic variations of timothy in tissue culture, calli were induced from mature seeds of four varieties, 'Colt', 'Chair', 'Richmond' and 'Hokuo' and plant regeneration frequency was compared. Significant differences were observed among the cultivars in both callus induction and plant regeneration. Genotype 'Colt' consistently performed best in the callus subculture and plant regeneration. The complete plantlets were thereafter transplanted to grow under greenhouse condition. Regenerated timothy plants were morphologically uniform with normal leaf and growth pattern.
Keywords
Callus; Plant regeneration; Timothy; Phleum pratense L.;
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1 Lee, S.-H., N. Ahsan, K.-W. Lee, D.-H. Kim, D.-G. Lee, S.S. Kwak, S.-Y. Kwon, T.H. Kim and B.-H. Lee. 2007. Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. J Plant Physiol. 164:1626-1638.   DOI   ScienceOn
2 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15:473-497.   DOI
3 김기용, 지희정, 이상훈, 이기원, 김원호, 정민웅, 서 성, 최기준. 2010. 이탈리안 라이그라스 춘파 재배에서 조.만성 품종간 생산성 비교. 초지조사료지. 30(2):97-102.
4 이효원, 김동암. 2003. 초지학. 한국방송통신대학교출판부. pp. 117-119.
5 최기준, 임영철, 지희정, 김기용, 박형수, 서 성, 문정섭, 김대호, 이상훈. 2010. 내재해 다수성 톨페스큐 신품종 "그린마스터"의 품종 특성. 한국초지조사료지 30(3):199-204.
6 Plazek, A., M. Filek and M. Wfdzony. 1999. Improvement of regeneration ability in Phleum pratense L. in vitro culture by dicamba. Acta Physiologiae Plantarum. 21:397-403.   DOI   ScienceOn
7 Schenk, R. U and A. C. Hildebrandt. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199-204.   DOI
8 Somers, D. A., D. A. Samac and P. M. Olhoft. 2003. Recent advances in legume transformation. Plant Physiol. 131:892-899.   DOI   ScienceOn
9 Spangenberg, G., Z. Y. Wang and I. Potrykus. 1998. Biotechnology in forage and turf grass improvement. In: Frankel et al. (Eds), Monographs on theoretical and applied genetics, Vol. 23, Springer Verlag, Heidelberg, p. 192.
10 Wang, Z.-Y., X.D. Ye, J. Nagel, I. Potrykus and G. Spangenberg. 2001. Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants. Plant Cell Rep. 20:213-219.   DOI
11 Lee, K.-W., S.-H. Lee, D.-G. Lee, D.-H. Kim and B.-H. Lee. 2006. Effect of orchardgrass varieties on callus culture and palnt regeneration. J. korean Grassl. Sci. 26:191-198.
12 Chen, L., C. Auh, P. Dowling, J. Bell, F. Chen, A. Hopkins, R. A. Dixon and Z.-Y. Wang. 2003. Improved forage digestibility of tall fescue (Festuca arundinacea) by transgenic down-regulation of cinnamyl alcohol dehydrogenase. Plant Biotechnol J. 1:437-449.   DOI   ScienceOn
13 Chen, L., C. Auh, P. Dowling, J. Bell, D. Lehmann and Z.-Y. Wang. 2004. Transgenic down-regulation of caffeic acid O-methyltransferase (COMT) led to improved digestibility in tall fescue (Festuca arundinacea). Funct Plant Biol. 31:235-245.   DOI   ScienceOn
14 Chu, C.C., C.S. Wang, C.C. Sun, C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic. 18:659-668.
15 Forster J.W. and G. Spangenberg. 1999. Forage and turf grass biotechnology: principles, methods and prospects. In: Setlow JK (eds), Genetic engineering: principles and methods, Vol 21, Kluwer Academic Publishers, New York, pp. 191-237
16 Guo, Y.D. and S. Pulli. 2001. Somatic embryogenesis and plant regeneration from suspension cultures of timothy (Phleum pratense L.). Act Agr Scand B-S P. 51:160-166.   DOI
17 Lee, K.-W., K.-Y. Kim, G.J. Choi, Y.C. Lim, W.H. Kim, M.W. Jung, S. Seo, B.-H. Lee and S.-H. Lee. 2008. Callus Induction and plant regeneration from mature seeds of timothy. J. Kor. Grassl. Forage Sci. 28:165-170.   과학기술학회마을   DOI   ScienceOn
18 Lee, K.-W., J.K. Lee, K.-Y. Kim, H.C. Ji, H.S. Park, K.-H. Kim, B.-H. Lee and S.-H. Lee. 2009a. Plant regeneration capacity of calluses derived from mature seed of perennial ryegrass cultivars. J. Kor. Grassl. Forage Sci. 29:285-290.   과학기술학회마을   DOI   ScienceOn
19 Lee, K.-W., G.J. Choi, K.-Y. Kim, H.C. Ji, H.S. Park, S.H. Yoon. and S.-H. Lee. 2009b. High frequency plant regeneration from mature seed derived callus of Italian ryegrass (Lolium multiflorum) cultivars. African Journal of Biotechnology. 8 (24), pp. 6828-6833.