Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Organ Formation-The Formation of Adventitious Roots, Trichomes and Calli from Leaf Segments of Arabidopsis thaliana by Naphthaleneacetic acid Concentrations, and Their Determination times

애기장대 (Arabidopsis thaliana) 잎 절편에서의 기관형성 특히 Naphthaleneacetic acid의 농도에 따른 부정근, 모용 및 캘러스 형성과 Determination Time

  • Published : 1999.07.01
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Abstract

The effect of auxins and cytokinins on the formation of adventitious shoots, adventitious roots, trichomes, and calli in MS basal medium was investigated in leaf segments from ecotype Columbia of Arabidopsis thaliana. Adventitious shoots, adventitious roots, trichome, and calli were formed from leaf segments by a wide range of hormone concentrations and combinations. Adventitious shoots were formed respectively in treatment with 0.1mg/L IAA and 10 mg/L BA. Adventitious roots were formed in treatments with low concentration of IAA and NAA. Trichomes and calli were formed by increasing the concentration of IAA and NAA. The optimal combination was 0.5mg/L NAA and 0.1mg/L BA for trichome formation, 10mg/L NAA and 10mg/L BA for calli formation. When NAA was treated alone in culture media, adventitious roots were formed in 0.1mg/L, trichomes were formed in 2.0mg/L, and calli were formed in 10mg/L. Inductive time for formation of adventitious roots, trichomes and calli were determined at 6,7 and 18 days respectively by periodical transfer of leaf segments from NAA containing medium to NAA free medium.

애기장대 (Arabidopsis thaliana) 생태종 'Columbia' 잎절편에서의 묘조, 부정근, 모용 및 캘러스 형성에 미치는 auxin과 cytokinin의 효과를 조사하였다. 광범위한 식물생장 조절물질 농도와 조합에서 묘조, 부정근, 모용 및 캘러스가 형성되었다. 묘조는 0.1mg/L IAA와 10mg/L BA에서 형성되었고, 부정근은 저농도의 IAA와 NAA에서 모두 형성되었다. 모용과 캘러스는 IAA와 NAA의 농도 증가에 따라 형성되었으나 모용은 0.5mg/L NAA와 0.1mg/L BA 혼합처리구에서 가장 많이 형성되었으며, 캘러스는 10mg/L NAA와 10mg/L BA혼합처리구에서 가장 많이 형성되었다. NAA의 단독 영향을 조사한 결과 부정근 형성은 0.1mg/L 모용은 2.0mg/L 캘러스는 10mg/L가 가장 효과적이었으며, NAA배지에 각각 일정 기간 잎 절편을 치상하였다가 NAA 무처리 배지에 옮긴 결과 부정근, 모용 및 캘러스을 형성하는 determination time은 각각 6, 7, 18일이었다.

Keywords

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