• Title/Summary/Keyword: 친화도 기법

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고정화된 enterokinase의 풀림과 재접힘 공정을 통한 효소 활성회복기법

  • Na, Se-Jin;Seo, Chang-U;Park, Sin-Hye;Lee, Eun-Gyu
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.513-516
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    • 2003
  • 고체상 풀림과 재접힘 공정을 통해서 EK의 활성이 회복되어지는 것을 확인할 수 있었다. 이는 고정화 효소를 사용함으로써 기존의 액상반응에서 불가능한 효소의 재사용 문제를 해결할 수 있다. 친화도보다는 다중 공유결합을 통한 효소의 고정화가 효소의 활성 회복에 높은 안정성을 가질 수 있다고 예상한다.

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Screening of Peptide Sequences with Affinity to Bisphenol A by Biopanning (바이오패닝에 의한 Bisphenol A 친화성 펩타이드 서열의 탐색)

  • Yoo, Ik-Keun;Choe, Woo-Seok
    • Korean Journal of Microbiology
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    • v.49 no.2
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    • pp.211-214
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    • 2013
  • Bisphenol A (BPA) is a highly hazardous component to human since it is regarded as one of endocrine disruptors. For the analysis and/or removal of BPA, the searching for the specific ligand with a selective affinity to target BPA is required. In order to find the peptide moiety that specifically binds to BPA, the ultrasound-assisted biopanning was carried out with a phage-displayed peptide library expressing constrained heptamer. After six rounds of positive screening against BPA particles followed by the negative screening against the surface of eppendorf tube, the peptide sequence (CysLysSerLeuGluAsnSerTyrCys) with affinity to BPA was screened based on the order of frequency from the screened phage clones. To further verify the specificity of screened peptide sequence, the cross-binding affinity of the phage peptide toward BPA analogues such as Bisphenol S (BPS) and Bisphenol F (BPF) was also assessed, where the selected phage peptide showed a higher affinity to BPA over BPS and BPF.

Study on the GIS Based Environmental Assesment Techniques for Environmental Friendly Road Route Plan (환경친화적인 도로노선 계획을 위한 GIS 기반 환경성 평가기법 연구 - 도로노선대 선정방법 개발 -)

  • Kang, Ho-Geun;Park, Tae-Kwen;Kim, Heung-Lae;Kim, Sang-Tae;Lee, Sang-Eun
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.3
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    • pp.223-231
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    • 2009
  • This study intends to develop a GIS-based environmental assesment technique for environmental friendly road route plan using a spatial analysis function. After embodying an impact range based on the analysis of factors which cause the environmental damage by the road construction, a literature survey on a diverse grading cases and legal regulations was conducted. As a quantitative model for the spatial analysis, AHP was selected. As the environmental assessment, 8 items such as topography & geology, animals & plants, soil ecology, water quality, air quality, noise, land use, and landscape were selected. and then finally classified into three-grades using a pairwise comparison of the alate collected by a questionnaire surveying, weighting values were applied to there 8 items. The highest weight value, the grade 1 among eight was 0.191 for animals & plants. As the result of verifying the above method for the case region, the most environment-friendly road route could be generated.

Efficient Video Streaming Based on the TCP-Friendly Rate Control Scheme (TCP 친화적인 전송률 제어기법 기반의 효율적인 비디오 스트리밍)

  • Lee, Jungmin;Lee, Sunhun;Chung, Kwangsue
    • Journal of Broadcast Engineering
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    • v.10 no.3
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    • pp.297-312
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    • 2005
  • The multimedia traffic of continuous video and audio data via streaming service accounts for a significant and expanding portion of the Internet traffic. This streaming data delivery is mostly based on RTP with UDP. However, UDP does not support congestion control. For this reason, UDP causes the starvation of congestion controlled TCP traffic which reduces its bandwidth share during overload situation. In this paper, we propose a new TCP-friendly rate control scheme called 'TF-RTP(TCP-Friendly RTP)'. In the congested network state, the TF-RTP exactly estimates the competing TCP's throughput by using the modified parameters. Then, it controls the sending rate of the video streams. Therefore, the TF-RTP adjusts its sending rate to TCP-friendly and fair share with competing TCP traffics. Through the simulation, we prove that the TF-RTP correctly estimates the TCP's throughput and improves the TCP-friendliness and fairness.