• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.10
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• pp.6715-6721
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• 2015

Recently, DNA data of missing person, killed person, and missing child continue to increase but most of statistical calculation for paternity confirmation is being done through manual methods or Excel. Therefore, we need development of a software which is able to facilitate both systematic management and effective analysis of Short Tandem Repeat (STR) derived from DNA data. Without extensive testing, through a twenty-month study was developed a web-based system which performs paternity analysis and kinship analysis easily based on the various options. The former uses an existing algorithm for paternity index and the latter does Identity by descent (IBD) formula. Due to our system validated over real datasets in terms of likelihood ratio and probability of paternity, it ensures increased reliability as well as effective management and analysis of DNA data in mass disaster. In addition, it includes advanced features such as an integrated environment, user-centered interface, process automation and so on.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.116-120
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• 2011

무심천에는 2002년부터 자연형 하천정비사업에 착수하면서 저수로, 저수로 하안, 고수부지 등 하도내에 여러 자연형 하천 공법과 함께 시설들을 설치하였다. 본 모니터링 조사사업은 '무심천 살리기'의 일환으로 이루어진 하천종합조사사업으로써 무심천의 수리/수환경 변화와 생태계의 변화를 조사를 통해 생태 수환경 수리특성 등 생태복원에 필요한 기초자료를 축적하고, 필요한 공법 등 향후 추진될 무심천 하천정비 및 관리에 반영하기 위하여 저수량, 홍수량, 생태계 및 하천공법 등에 대한 조사 및 분석을 전문가, 시민참여 그리고 공무원이 함께 수행하고 있다. 본 연구에서는 무심천을 친자연형 하천으로 복원하기 위하여 실시한 친자연형공법에 대하여 모니터링을 실시하고 얻은 수리/공학적 그리고 생태적 성과물을 자연형하천정비사업에 피드백시키는 자연형 하천관리방안에 대하여 논하였다. 무심천의 친자연형하천관리를 위하여 하천의 3대기능과 관련한 항목을 이용하여 평가지표를 설정하고, 평가를 하고 자연형 하천공법 등급을 I-V로 구분하여 하천관리를 위한 기준으로 사용하였다. 평 저수시 무심천 본류와 지류에 대한 유량측정을 실시하고, 하천유지유량을 산정하였다. 저수/고수호안, 하상, 둔치에 대한 조사분석을 실시하고 관리방안을 제시하여 무심천을 점차 자연형하천으로 변화시키고 있다. 또한 생태계의 적응관리로 하천식생을 유지하기 위한 관리를 실시하고, 유해식물과 외래식물을 제거하고, 하도내 직경이 10(m) 이상되는 버드나무 수목에 밑둥치기를 실시하여 치수기능의 저해를 방지하였다.

• Journal of Life Science
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• v.30 no.10
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• pp.912-918
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• 2020

The Korean native black goat (Capra hircus coreanae) is the goat species to be officially registered in Korea under the Food and Agriculture Organization. The object of this study is to establish a set of microsatellite (MS) markers for the individual identification and parentage verification of goats. In this study, we analyzed alleles of MS markers in crosses between Korean native black goats and crossbred goats (n=304 animals), and, based on the diversity of alleles for each marker, we selected 11 MS markers for individual identification and parentage verification. Using these 11 MS markers, the probabilities of different individuals with the same genotype being found within random and half-sib mating populations were 5.58×10^-10 and 1.15×10^-7, respectively. The parentage verification accuracy was 0.999996 when information about the parents was available and 0.999833 with no information. Thus, even given the total rearing population of 576,150 animals in South Korea, we concluded that these markers could be used for the individual identification and parentage verification of goats. Moreover, by analyzing the genetic relationships between the four lines of Korean native black goats and the crossbred goats, we verified the genetic characteristics of Korean native black goats, confirming their conservation value as a unique genetic resource.

• Journal of the korean veterinary medical association
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• v.34 no.4
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• pp.232-237
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• 1998

• The Korean Journal of Malacology
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• v.30 no.4
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• pp.383-390
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• 2014

The multiplex PCR system including six microsatellites from Haliotis discus hannai, consisting of dinucleotide and trinucleotide repeat units, is developed. The six loci were coamplified in a single reaction employing dye-labeled primers. Alleles from these loci were sized using an internal standard by automated sample processing in an ABI3100 Genetic Analyser. Amplified alleles in profiles containing selected microsatellites were typed clearly, providing easily interpretable results. In this results suggest that the presented multiplex PCR system may be a useful tool in a selective breeding program of H. discus hannai in which genetic identification will allow different genotypes to be reared together from fertilization. This should have a great impact as it will make selective breeding more efficient. Moreover, it will be useful in a variety of applications, including strain and hybrid identification, parentage assignment, pedigree reconstruction, estimating genetic diversity and/or inbreeding.

• Journal of Life Science
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• v.16 no.3 s.76
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• pp.382-386
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• 2006

A total number of 20 cat samples including 8 parentage testing and 12 individual identification were genotyped. Genomic DNA was extracted from buccal swab, and genotyped by using 10 microsatellite markers (FCA005, FCA26, FCA224, FCA240, FCA453, FCA293, FCA075, FCA105, FCA229, and FCA651). This method consisted of single PCR procedure and showed reasonable amplification of all PCR products. Genotypes were determined by genetic analyzer. The number of alleles per locus of cats varied from 3 to 8 with a mean value of 5.5. Expected heterozygosity was ranged from 0.390 to 0.827 (mean 0.639) and the total exclusion probability of 10 microsatellite loci was 0.9441. Of the 10 markers, FCA240 marker has relatively high PIC value (>0.7). Of the 8 cats, 7 cats were qualified by compatibility according to the Mendelism. These results can give basic information for developing parentage verification and individual identification system in cat.

• Journal of Veterinary Clinics
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• v.26 no.3
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• pp.220-225
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• 2009

The present study demonstrates a new approach that enables effective horse parentage testing using 22 ISAG microsatellite markers involving 6 heads of Thoroughbred horse(TB) and non-TB. In the comparison allele distribution between these horses, the alleles found in the TB were numerously detected in the non-TB. As results, we confirmed that these ISAG microsatellite markers might apply the pedigree registration of Korean native horse(Jeju horse).

• Korean Journal of Environment and Ecology
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• v.11 no.2
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• pp.177-200
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• 1997

This study has been conducted to propose the guidelines for development of ecological urban open space in Korea. The eastern area of Japan was studied as case area. It was classified into sex biotope types and the project background of each sites was analyzed. The contents and technique on the basis concept, survey of environmental and biotic condition planning and design(zoning and use planning, target selection and management), preparation and management of natural environment and management, and monitoring was analyzed. It was planned out the projects in order to create the nature experience space in urban area. It was found that development of ecological urban open space by the biotope creation technic was based on three types(environmental transfer, environmental creation, and environmental inprovement type). Also most cases of projects created the biotope by the conservation of the present enviroment and ecosystem. When the development of ecological urban open space was planned, selection of target species was seemed to very important that clearly propose to the guidelines.

• Korean Journal of Environment and Ecology
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• v.20 no.2
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• pp.170-187
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• 2006

The purpose of this study is to provide a basic datd for establishing a basic park management plan for Bulamsan Urban Nature Park. This research surveys very closely about the present condition of the natural ecosystem based on the examination and analysis of the present condition. This research suggests development and management methods for environment-friendly park by understanding damages on the ecosystem due to overuse, as well as park facilities and their effects on the surrounding environment, using quantative analysis. The three steps: researching the present condition research, ecosystem evaluation, and park management plan were taken in order. The present condition research is based on the general condition, natural environment, and natural ecosystem data. In the ecosystem evaluation, wildlife habitats evaluation, landscape evaluation, and biotope type research which is a materialized form of the plant ecosystem were excuted based on the present condition research data. In the park management plan step, 1) classified ecosystem and landscae preservation zone, ecosystem protection zone and ecosystem restoration zone as ecosystem and landscape preservation plan, 2) plannings for ecosystem, fine landscape preservation, damaged area restoration and recovery plan were established in order to suggest environment-friendly plan,

• Proceedings of the Korea Water Resources Association Conference
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• 2009.05a
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• pp.452-456
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• 2009

무심천에 대한 모니터링은 '무심천 살리기'의 일환으로 이루어진 하천종합조사사업으로써 저수량, 홍수량, 생태계 및 하천공법 등에 대한 조사 및 분석을 수행하고 있다. 모니터링은 5년간에 걸친 사업으로 2007년 5월부터 시작하여 2009년 현재 2차년도 사업을 진행중이다. 본 연구에서는 특히 무심천에 적용된 하천공법에 대한 조사 및 분석에 국한하였다. 여기서 2002년부터 자연형 하천정화사업에서 적용했던 공법과 이로 인한 수리인자의 변화를 관찰하고, 저수로 호안을 포함한 각종 하천환경 시설물의 수리적 대응도를 분석하여 개선 방안을 제시한 것이다. 무심천의 조사구간은 미호천 합류부로부터 청원군과의 경계인 지북동 경계점 까지이며, 조사 및 분석한 하천연장은 무심천 본류 12.0 km이다.