• Korean Journal of Microbiology
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• v.40 no.3
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• pp.237-243
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• 2004

The aim of this study is to investigate the detection rate of putative periodontopathogens, Porphyromonas gingivalis, Tannerella forsythia, and Actiobacillus actinomycetemcomitans, related to cardiovascular diseases(CVD). Plaques were sampled from 15 subjects (4 sites of denture base and/or tooth) with sterilized explorers and were transported in IX PBS. The detection of periodontopathogens was performed by polymerase chain reaction with species-specific primers based on 16S rDNA. The PCR products were cloned into pGEM-T easy vector and its nucleotides were sequenced in order to confirm the specificity. Our data showed that the detection rate of P. gingivalis and T forsythia in denture base of edentulous patients was 25％ and 75％, respectively. And the detection rate of P. gingivalis and T.forsythia in denture base of patient having one more tooth was 91％. The results indicate that plaque of denture base may serve as reservoirs of oral bacteria related to CVD.

• The Journal of the Korean dental association
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• v.34 no.1 s.320
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• pp.65-71
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• 1996

• Journal of Korean society of Dental Hygiene
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• v.24 no.2
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• pp.131-139
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• 2024

Objectives: Oral bacterial samples included subgingival, supragingival, and saliva plaques. As the diversity and number of microorganisms deffer depending on the area of the oral cavity and the method used, an appropriate and reliable collection method is important. The present study investigated oral bacterial sampling methods. Methods: Supragingival dental plaque was collected from the buccal and lingual tooth surfaces of study participants using sterilized cotton swabs. Plaques were collected from the subgingival area using a sterilized curette. Bacterial genomic DNA was extracted using MagNA Pure 96 DNA and Viral NA low-volume kits. Real-time polymerase chain reaction (PCR) was performed using the PowerCheck^TM Periodontitis Pathogens Multiplex Real-time PCR kit. Results: Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Fusobacterium nucleatum of the orange complex were not observed in the subgingival biofilms of all study participants. For Porphyromonas. gingivalis, a significant correlation was observed between supragingival, subgingival, and total tooth surface biofilms. Compared to the supragingival and subgingival biofilmss, total tooth surface biofilm exhibited the highest bacterial count when the inswabbing method was used. Conclusions: Based on these findings, the supragingival swab method is recommended for oral bacterial research.

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.4
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• pp.522-530
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• 2009

The purpose of this study was to evaluate the clinical effectiveness of new streptococcus detection system which used monoclonal antibody against Streptococcus mutans. 92 children aged between 2 and 8 were involved in this experiment and their saliva samples were collected for testing. Streptococcus mutans were measured by both monoclonal antibody-based detecting system (Saliva-$check^{TM}$ Mutans) and dip slide detecting system($Dentocult^{TM}$-SM). The results showed that Saliva-$check^{TM}$ Mutans levels had a significant correlation with dfs rate of subjects and the two test kits, Saliva-$check^{TM}$ Mutans and $Dentocult^{TM}$-SM were shown to have a good correlation although they were based on different mechanism.

• Journal of the korean academy of Pediatric Dentistry
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• v.47 no.2
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• pp.120-127
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• 2020

The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer. Multispecies cariogenic biofilms containing Streptococcus mutans, Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 μM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM. This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.

• Journal of the Korea Society of Computer and Information
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• v.27 no.6
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• pp.131-137
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• 2022

As the age increases, the oral cavity, that is, the teeth and periodontium, also begin to age, and accordingly, a preparation process is required. The preparation process is an important period for oral health management to start continuously with oral health education consisting of knowledge, attitude, and behavior from the 20s. Therefore, to design a clinical dental hygiene course for patients who visited a dental clinic in Gyeonggi-do and received continuous care in an oral health care room after treatment, we tried to analyze the data of the dental hygiene assessment. As a dental hygiene assessment tool, based on personal information and general medical history, dental visit experience, bleeding on probing(BOP), bad breath measurement, phase contrast microscopy, and O'Leary index were performed. The number of subjects who had dental visits was 75.4% and those without experience were 24.6%, and as a result of the periodontal examination, generally bleeding was found in 76.3%. In preventive oral care, the stage of dental hygiene assessment in the 20s is an important first step. From this point on, it is an important time to be systematically habituated so that you can take responsibility for your own oral condition. Therefore, in this study, the results of dental hygiene assessment through oral examinations of subjects in their 20s are derived and presented as basic data for the development of dental hygiene performance competency of dental hygienists during the clinical dental hygiene process in oral health education and oral health management.

• Journal of dental hygiene science
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• v.12 no.2
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• pp.163-170
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• 2012

The purpose of this study was determine the effects of professional tooth cleaning and plaque control instruction(PT & PCI) on reduction of peri-implantitis. A total 80 implant patients were investigated using O'Leary plague index(PI), L${\ddot{o}}$e & Silness gingival index(GI), implant bone loss(BL). While the PT & PCI was conducted by using 'Watanabe method' after scaling for the experimental group, the engine polishing and a plaque control instruction was operated by rolling method after scaling for the control group. The collected data were analyzed with t-test, ANOVA, paired t-test and stepwise multiple regression. PI and GI of the experimental group significantly decreased than the control group(p<0.05). BL, also tended to decreased in the experimental group than the control group(p=0.155). Multiple regression analysis, the factors that was highly correlated with PT & PCI on the PI and GI. According to the implant characteristics, implant location and duration were closely related to PI(p<0.05). Therefore, the PT & PCI is one of the effective methods to reduce the failure caused by the peri-implantitis. Based on this conclusion, that it would be meaningful if the proposed PT & PCI is applied to the oral health management programs of the implant patients.

• Journal of the korean academy of Pediatric Dentistry
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• v.26 no.1
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• pp.14-24
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• 1999

The purpose of study was to develop new dental plaque disclosants which could replace erythrosine. Three food coloring agents(Red No.40, Blue No.1, and Mixed Green), erythrosine and fluorescein were tested for their color difference, antibacterial property, and biocompatibility. Color difference of Red No.40 was greater than that of erythrosine as concentration of solution increased. Color differences of Blue No.1 and Mixed Green were smaller than that of red dyes. Erythrosine showed obvious antibacterial property, but food coloring agents showed almost no antibacterial property. The taste and sensation of erythrosine was the worst, and the taste of Red No.40 and the sensation of Mixed Green were the most tolerable. Erythrosine stained dental plaque and oral soft tissue most deeply and long, and Blue No.1 was the next in the depth and longevity of stain.

• The Journal of the Korea institute of electronic communication sciences
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• v.11 no.7
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• pp.701-706
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• 2016

The difference of oral environment improvement effect of the test dentifrice was examined by applying the Centella Asiatica extract on the oral dentifrice composed by above ingredients. And by using the double blind manner the plaque, gingival index and probing pocket depth were measured and the corresponding changes were set to be verified.

• Microbiology and Biotechnology Letters
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• v.21 no.3
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• pp.263-268
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• 1993

Seventeen strains were isolated from soil, cattle rumen, cereal sewage dregs, insect on agar plate containing insoluble glucan as a sole carbon source from immobilized Streptococcus mutans, which produced alpha-1,3 glucanase for lysis of dental plaque. Among these strains isolated from soil, SW-522 and SW-713 that had appeared to produce the high level of alpha-1,3 glucanase, degraded insoluble glucan from S. mutans 97.6% and 49.4%, respectively in 5 hours. The activity of crude alpha-1,3 glucanase from SW-522 was 1.3mg insoluble glucan/min.mg protein. This enzyme was entirely degraded insoluble glucan on glass tube which produced by S. mutans in TH medium with 5% sucrose.