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Detection Rate of Periodontopathogens Associated with Cardiovascular Diseases in Denture.  

Lim Mi-Young (조선대학교 치과대학 보철학교실)
Kim Hwa-Sook (조선대학교 치과대학 구강생화학교실)
Jeong Jae-Heon (조선대학교 치과대학 보철학교실)
Yang Ji-Youn (조선대학교 치과대학 보철학교실)
Oh Sang-Ho (조선대학교 치과대학 보철학교실)
Kook Joong-Ki (조선대학교 치과대학 구강생화학교실)
Publication Information
Korean Journal of Microbiology / v.40, no.3, 2004 , pp. 237-243 More about this Journal
Abstract
The aim of this study is to investigate the detection rate of putative periodontopathogens, Porphyromonas gingivalis, Tannerella forsythia, and Actiobacillus actinomycetemcomitans, related to cardiovascular diseases(CVD). Plaques were sampled from 15 subjects (4 sites of denture base and/or tooth) with sterilized explorers and were transported in IX PBS. The detection of periodontopathogens was performed by polymerase chain reaction with species-specific primers based on 16S rDNA. The PCR products were cloned into pGEM-T easy vector and its nucleotides were sequenced in order to confirm the specificity. Our data showed that the detection rate of P. gingivalis and T forsythia in denture base of edentulous patients was 25％ and 75％, respectively. And the detection rate of P. gingivalis and T.forsythia in denture base of patient having one more tooth was 91％. The results indicate that plaque of denture base may serve as reservoirs of oral bacteria related to CVD.
Keywords
cardiovascular disease; denture; porphyromonas gingivalis; Tannerella forsythia;
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