• Title/Summary/Keyword: 충성

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Insect Resistance of Tobacco Plant Expressing CpBV-ELP1 Derived from a Polydnavirus (폴리드나바이러스 유래 CpBV-ELP1 발현 담배의 내충성)

  • Kim, Eunseong;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.56 no.1
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    • pp.19-28
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    • 2017
  • Polydnaviruses (PDVs) are a group of double-stranded DNA viruses symbiotic to some endoparasitoid wasps. Cotesia plutellae bracovirus (CpBV) is a PDV symbiotic to an endoparasitoid wasp, C. plutellae, parasitizing young larvae of Plutella xylostella. An early expressed gene, CpBV-ELP1, plays an important role in the parasitism by suppressing host cellular immunity by its cytotoxic activity against hemocytes. This study aimed to test its oral toxicity against insect pest by expressing it in a recombinant tobacco plant. A recombinant CpBV-ELP1 protein was produced using a baculovirus expression system and secreted to cell culture medium. The cell cultured media were used to purify CpBV-ELP1 by a sequential array of purification steps: ammonium sulfate fractionation, size exclusion chromatography, and ion exchange chromatography. Purified rCpBV-ELP1 exhibited a significant cytotoxicity against Spodoptera exigua hemocytes. CpBV-ELP1 was highly toxic to the fifth instar larvae of S. exigua by injection to hemocoel. It also showed a significant oral toxicity to fifth instar larvae of S. exigua by a leaf-dipping assay. CpBV-ELP1 was cloned into pBI121 vector under CaMV 35S promoter with opaline synthase terminator. Resulting recombinant vector (pBI121-ELP1) was used to transform Agrobacterium tumefaciens LBA4404. The recombinant bacteria were then used to induce callus of a tobacco (Nicotiana tabacum Xanthi) leaves and subsequent generation (T1) plants were selected. T1 generation tobacco plants expressing CpBV-ELP1 gave significant insecticidal activities against S. exigua larvae. These results suggest that CpBV-ELP1 gene can be used to control insect pests by constructing transgenic crops.

A Study on the Effect of Web Site Characteristics in to Customer Performance - with the focus on the modulating effect of promotion - (웹 사이트 특성이 고객성과에 미치는 영향에 관한 연구 - 판매촉진 활동의 조절효과를 중심으로 -)

  • Hong, Sang-Jin;Rha, Dong-Kyun;Jang, Hyung-Sub
    • Management & Information Systems Review
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    • v.29 no.2
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    • pp.75-96
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    • 2010
  • In the recent years the society has entered the stage of informatization, the base of informatization has expanded, and the internet users have exploded, which has led to revolutionary changes to all across business management of the world. The influences of customer value and satisfaction were reviewed on a web site, and their subsequent impacts on customer loyalty were suggested. It was also analyzed how the concept of sales promotion that was set as a moderating variable affected the characteristics of a web site, customer value and satisfaction, and correlations between customer value and satisfaction. Finally based on the analysis results, practical strategic alternatives were suggested. Accordingly a web site provider can't afford to ignore sales promotion just because it doesn't have modulating effect because the customers will turn to his or her competitors. It's important to make ongoing efforts into techniques of sales promotion to meet the expectations of clients.

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A Study on The Effect of Organizational Culture on Job Satisfaction and Organizational Commitment In ICT Enterprises (ICT 기업의 조직문화가 조직구성원 직무만족과 조직몰입에 미치는 영향에 관한 연구)

  • Lee, Yoeng-Taak
    • Management & Information Systems Review
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    • v.36 no.4
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    • pp.149-166
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    • 2017
  • The purpose of this study puts stress on effects of job satisfaction and organizational commitment depending on the type of organizational culture of ICT enterprises. This study utilized the 6th Human Capital Corporate Panel(HCCP) data from Korea Research Institute for Vocational Education & Training to analyze the effects of organizational culture in ICT enterprises on the job satisfaction and organizational commitment. The Samples are managers, supervisor, and employees in ICT industries who replied thorough the 6th HCCP. Answers from 875 people, except inappropriate answers, were used to test a hypothesis. In order to do that, reliability analysis and correlation analysis and regression analysis, utilizing the SPSS 24.0 & Amos 18.0, were used to analyze the effects of organizational culture on the job satisfaction and organizational commitment in ICT enterprises. With the purpose of this study, organizational cultures in ICT enterprises have different effects on job satisfaction and organizational commitment. The group culture, development culture, rational culture and hierarchy culture have a positive effects on job satisfaction. And the group culture, development culture and rational culture have a positive effects on organizational commitment. Whereas, hierarchy culture have no effects on organizational commitment. Also, job satisfaction have a positive effects on organizational commitment. Among four cultures of ICT enterprises, the importance of group culture should be stressed. According to the result of empirical analysis, group culture has the most positive impact on job satisfaction, contrary to the expectation that development culture might be the one. So far, the group culture, which emphasizes organizational flexibility, integration, trust, teamwork, high participation, royalty and morale, have positive impact on the organizational employees the most.

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Comparison of Growth Performance of Selected ×Populus alba·glandulosa Clones in Different Locations (×Populus alba·glandulosa 우량개체(優良個體)의 지역별(地域別) 생장비교(生長比較))

  • Son, Doo Sik
    • Journal of Korean Society of Forest Science
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    • v.39 no.1
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    • pp.64-68
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    • 1978
  • Thirty nine clones of ${\times}$Populus alba glandulosa were selected tentatively and planted in Hwasung in Kyunggi-do, Jinju in Kyungnam-do, Myongju in Kangweon-do. Height measurements after tree growing seasons, indicated significant differences between clones and locations. Interaction between clones and locations were not significant statistically. Among 39 clones, 65-29-19, 65-11-106, 67-47-10, 66-14-149, 64-6-44 were best performing clones. Height measurements in Hwasung Kyunggi, and Jinju Kyungnam were 3.83m, 4.02m respectively, while that in Myongju Kangweon was only 1.06m. Myongju plantation in Kangweon-do is located in altitude of 800m and its average annual temperature was $6.2^{\circ}C$. Retarded growth in this plantation is caused high altitude and low temperature of the plantation which resulted from nearly three monthes shorter growing period than two other plantations. It is also recommended that straightness of stem, branching, wood quality and insect and disease-resistance have to be considered as selection criteria along with growth performance.

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A Study of Influences of Fairness Perception on Perceived Organizational Support, Organizational Commitment and Organizational Citizenship Behavior (구성원의 공정성 지각이 영리조직과 비영리조직의 조직시민행동에 미치는 영향에 관한 연구)

  • Min, Nam-Sik;Lim, Jung-Sook
    • Korean Business Review
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    • v.22 no.1
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    • pp.45-75
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    • 2009
  • Previous researches of organizational citizenship behavior have been focused mainly on organizations, and there have been only a few studies on the organizational citizenship behavior of nonprofit organizations. In particular, few empirical studies have been made on the relations among variables affecting the organizational citizenship behavior of profit and nonprofit organizations. Thus, this study attempted comparative analysis. According to the result of the analysis, in profit organizations, the fairness of procedure, emotional commitment and continuous commitment had a significant effect on organizational citizenship behavior, as well as the fairness of distribution and perception of organizational support. In nonprofit organizations, on the contrary, the fairness of distribution, the fairness of procedure, and perception of organizational support were insignificant, and only emotional commitment and continuous commitment had a significant effect on organizational citizenship behavior. This study is meaningful in that it comparatively analyzed the effects of organization members' perception of fairness on organizational citizenship behavior in profit and nonprofit organizations, and its outcome may be utilized as a reference in future researches but it has a problem in external validity that poses a limitation in its generalization.

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A Study on Segmenting of Cruiser Customers (관광유람선 고객의 시장세분화에 관한 연구)

  • Lee, Jun-Hyunk
    • Journal of Global Scholars of Marketing Science
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    • v.16 no.1
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    • pp.73-91
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    • 2006
  • This study was conducted for market segmentation of cruise tourist according to launching the "T" in Busan. Benefit segmentation was used to identify attributes of cruise services; importance of ship's physical appearance and importance of service and activities. 24 attributes were distilled to 5 factors: 'Facility & Service', 'Atmospherics of cruise ship', 'Escape', 'Choice', 'Safety'. A K-means cluster analysis identified three clustered segments for five importance factors in which high loyalty customers were found to be the most important segment. Based on the findings, three distinct groups were formed: 'Moderators', 'High Loyalty', 'Spurious'. The most important factors by high loyalty groups were identified 'Safety', 'Facility & Service', 'Atmospherics of cruise ship', 'Choice', 'Escape' in order. The results of the study showed statistically significant differences among the three groups in terms of demographic and behavioral variables. Especially, the target market should be considered by 'High Loyalty' group and 'Moderators' group in order. Positioning strategies and marketing mix strategies for effectively targeting the segments were discussed.

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Effects of hydrogen plasma on the formation of self-organized InAs-quantum dot structure (자기조직화에 의한 InAs 양자점 구조 형성에 미치는 수소플라즈마의 효과)

  • ;;;K. Ozasa;Y. Aoyagi
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.6 no.3
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    • pp.351-359
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    • 1996
  • We have investigated the effect of hydrogen plasma on the formation of InAs QDs (quantum dots) structure by using a CBE (chemical beam epitaxy)system equipped with ECR (electron cyclotron resonance) plasma source. It is confirmed that the formation of self-organized InAs-QDs on GaAs is started after the growth of InAs layer up to 2.6 ML (monolayer) with the irradiation of hydrogen plasma while it is started after 1.9 ML without hydrogen gas and hydrogen plasma through the observation of RHEED patterns. Density and size of the QDs formed at $T_{sub}=370^{\circ}C$ are $1.9{\times}10^{11}cm^{-2}$ and 17.7 nm without hydrogen plasma, and $1.3{\times}10^{11}cm^{-2}$ and 19.4 nm with hydrogen plasma, respectively. It is also observed from the PL(photoluminescence) measurement on InAs-QDs that red shift in PL peak energy and broadening in FWHM (full width at half maximum)of PL peak caused by the effects of hydrogen plasma on the increment of size and its distribution. These effects of hydrogen plasma are considered as a act of atomic hydrogen which enhances the layer-growth of InAs on GaAs resulted from the relief of misfit strain between GaAs substrate and InAs.

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The Relationship between Organizational Commitment and Intention to Leave and the Moderating Effect of Gender and Occupation in Dae-deok Venture Enterprises (조직몰입과 이직의도의 관계 및 성별과 직종의 조절효과: 대덕 벤처기업을 중심으로)

  • Kim, Jung Seok;Ahn, Kwan Young
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.7 no.4
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    • pp.77-86
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    • 2012
  • The purpose of this paper is to review the relationship between organizational commitment, and voluntary turnover(intention to leave), and the moderating effects of personal factors(gender and occupation) on the relationship between organizational commitment and intention to leave. In order to verify and achieve the purposes mentioned above, questionnaire data are gathered and analysed from 458 employees in Daedeok Innopolis Venture Enterprises, Dae-jeon Metropolis, South Korea. Multiple regression and multiple hierarchical regression analysis are applied to test the proposed hypotheses: 1) the relationship between organizational commitment(affective commitment, continuance commitment and normative commitment) and intention to leave, 2) the moderating effects of personal factors(gender and occupation) on the relationship between organizational commitment and intention to leave. Empirical survey's findings are as follows; First, affective commitment(AC) and continuance commitment(CC) are related negatively with intention to leave(IL), but normative commitment(NC) is not related significantly with IL. Second, gender positively moderates the relationship between affective commitment and intention to leave, and occupation negatively moderates the relationship between continuance commitment and intention to leave.

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A Study on the Relationship between Marketing Activities and Brand Equity in Nonprofit Organizations : Focused on the Donation Market of Charitable Organizations (비영리조직의 마케팅 활동과 브랜드 자산 간 관계에 관한 연구: 자선모금기관의 기부시장(donation market)을 중심으로)

  • Lee, Dong-Young;Byun, An-Gie
    • Korean Journal of Social Welfare
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    • v.59 no.2
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    • pp.303-325
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    • 2007
  • This study explores the relationship between marketing activities and brand equity in nonprofit organizations, specifically investigating the relational linkage between four selected marketing mix elements and brand equity through the mediating role of brand equity dimensions. Employing a structural equation model, the study empirically tests research hypotheses and finds some important implication for brand equity creation strategies for nonprofits. The results show that brand association is the only dimension that is positively related to brand equity, reflecting the current donation market in Korea where nonprofits have such a low presence that donors have little knowledge and chance to identify their brands and to build trust relationships with them. Furthermore, the results highlight the importance and roles of promotion and process marketing efforts, as they are proved to enhance brand association. The study also finds that promotion is positively related to brand awareness and process contributes to perceived quality and brand loyalty. Based on these findings, the study suggest that nonprofits make further efforts on active promotion and process development to create positive, differentiated images and better accessibility, and finally improve their brand equity.

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Effects of Airport Special Guard's Security Service Value on Customers' Satisfaction and Customers' Loyalty (공항특수경비원들의 보안서비스가치가 고객만족 및 고객충성도에 미치는 영향)

  • Lee, Ji Eun;Kim, Chan Sun
    • Convergence Security Journal
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    • v.15 no.1
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    • pp.37-47
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    • 2015
  • This study aims at exploring effects of airport special guard's security service value on customers' satisfaction and customers' loyalty. Users of airport facilities located in Incheon were selected as the population in Jun. 2013 and the sample was extracted using cluster random sampling and 460 people were used for a final analysis. The questionnaire used for the study was composed of total 22 items including 6 items of socio-demographical characteristics, 6 items of security service value, 6 items of customers' satisfaction and 4 items of customers' loyalty etc. and using SPSSWIN 18.0, factor analysis, reliability analysis, multiple regression analysis, path analysis etc. were used. The credibility of the questionnaire was indicated to be over ${\alpha}$=.809. The findings are as follows. First, security service value has an effect on customers' satisfaction. if monetary value is enhanced, satisfaction with service composition is high. second, security service value has an effect on customers' loyalty. If monetary value and time value are enhanced, will of continuous use and will of recommendation of others are high. Third, customers' satisfaction has an effect on customers' loyalty. If satisfaction with service composition and satisfaction with use decision are increased, will of continuous use and will of recommendation of others are high. Fourth, airport special guard's security service value has a direct and indirect effect on customers' satisfaction and customers' loyalty. That is, it means customers' satisfaction is an important variable to mediate security service value and customers' loyalty.