• Applied Biological Chemistry
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• v.32 no.3
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• pp.209-215
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• 1989

Changes of protein contents and amino acid composition and SDS-PAGE pattern of protein of mung bean which were germinated in dark at $25^{\circ}C$ for 6 day. The total protein contents gradually decreased during germination and the contents of each fractionative soluble proteins were increased shortly after the soaking of mung bean and gradually decreased during the germination afterwards. SDS-PAGE of albumin fraction showed 18 bands, and during the germination the most of bands were diminished or disappeared. But protein bands at 24,000, 40,000, 45,000, 70,000 dalton position remained until 6th day of germination. SDS-PAGE of globulin fraction showed 6 discrete bands, and during the germination the protein band at 45,000 dalton position disappeard. But the protein bands at $14,000{\sim}25,000$ dalton position did not change during the period. SDS-PAGE of glutelin fraction showed 10 discrete bands, and during the germination the bands of $45,000{\sim}70,000$ dalton become diminished or disappeared. But the bands of 30,000, 60,000 dalton did not change throughout the germination period.

• Journal of the Korea Organic Resources Recycling Association
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• v.19 no.1
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• pp.93-101
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• 2011

The modeling of SDR was carried out for the application of the solid waste incineration system. To find optimum operating condition for removal of acidic gases, computation fluid dynamic(CFD) model was used. In this study, the temperature profile of SDR(spray dry reactor) and the gas velocity profile for different models were investigated. In this model, the diameter of SDR was 3 meter and the height of SDR was 9 meter. The amount of inlet combustion gas of SDR was $6,125Nm^3/hr$ and the inlet temperature of SDR was 493 K. The amount of lime injection of SDR was 151 kg/hr. When the inlet shape of SDR was changed, the temperatur of SDR was changed and the gas velocity of SDR was 0.48 m/sec to 1.17m/sec and the outlet gas velocity of SDR was 6.9 m/sec to 7.42m/sec As a result of modeling, the average velocities in SDR and outlet were 0.489 m/sec and 7.424 m/sec, respectively, in which the temperature of outlet in SDR was 448 K.

• Membrane Journal
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• v.31 no.1
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• pp.52-60
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• 2021

When preparing calcium alginate microspheres using rotating membrane emulsification that rotates SPG (Shirasu porous glass) tubular membrane in the continuous phase, the optimal conditions of rotating membrane emulsification process parameters for producing monodisperse microspheres were determined. We determined the effects of process parameters of rotating membrane emulsification (the rotating speed of membrane module, the transmembrane pressure, the ratio of dispersed phase to continuous phase, the alginate concentration, the emulsifier concentration, the stabilizer concentration, the crosslinking agent concentration, and the membrane pore size) on the mean size and size distribution of alginate microspheres. As a result, the size of the microspheres decreased as the rotating speed of membrane module, the emulsifier concentration, and the crosslinking agent concentration increased among the process parameters of rotating membrane emulsification. On the contrary, as the ratio of dispersed phase to continuous phase, the transmembrane pressure, and the alginate concentration increased, the size of the microspheres increased. In the rotating membrane emulsification using an SPG membrane with a pore size of 3.2 ㎛, it was possible to finally prepare monodisperse alginate microspheres with a particle size of 4.5 ㎛ through the control of process parameters.

• Journal of Korea Port Economic Association
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• v.37 no.3
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• pp.75-91
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• 2021

Due to increased competition in supply side to reduce operational costs, port professionals have experienced extreme pressure, which demanded academicians to develop the model for efficient port operations from the industry perspective. Among many ports in the world, U.S. ports are our primary interest to analyze in our study for its high volume of cargoes transacted in the U.S. ports. We primarily employed DEA (Data Envelopment Analysis) technique to research the productivity of U.S. ports and applied the algorithm of double bootstrapped DEA proposed by Simar & Wilson (2007) to further investigate the driving forces of the performance of U.S. port operations. The external variables employed in our study comprise onDock Rail, Channel Depth, Location, Area, Acres, ForeignCargoRatio, and TEUChange, out of which onDock Rail, Acres, ForeignCargoRatio, and TEUChange were significant. In order to evaluate the effects of methodology selection, we conducted the same analysis applying the Censored model (Tobit) and contrasted the outcomes drawn from the two different techniques. Based on the findings from this work we proposed managerial implications and concluded.

• Microbiology and Biotechnology Letters
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• v.48 no.3
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• pp.344-357
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• 2020

Strain improvement and bioprocess development were undertaken to enhance hyaluronic acid(HA) production by Streptococcus zooepidemicus cells. Using a high-yielding mutant strain, statistical medium optimization was carried out in shake flask cultures, resulting in 52% increase in HA production (5.38 g/l) at the optimal medium composition relative to the parallel control cultures. For sufficient supply of dissolved oxygen (DO), which turned out to be crucial for enhanced production of HA, agitation system and speed were intensively investigated in 5 L bioreactor cultures. Increase in oxygen mass transfer coefficient (k_La) through increment of agitation speed (rpm) and 35% expansion of diameter of the newly-designed impellers showed significantly positive effects on HA production. By installing an expanded Rushton-turbine impeller for efficient break-down of sparged air, and an extended marine impeller above the Rushton-turbine impeller for efficient mixing of the air-born viscous fermentation broth, maximum amount of HA (9.79 g/l) was obtained at 450 rpm, 1.8 times higher level than that of the corresponding flask culture. Subsequently, the possibility of bioprocess scale-up to a 50 L bioreactor was investigated. Despite almost identical maximum HA production (9.11 vs 9.25 g/l), the average HA volumetric productivity (r_p) of the 50 L culture turned out only 74% compared to the corresponding 5 L culture during the exponential phase, possibly caused by shear damages imposed on the producing cells at the high stirring in the 50 L culture. The scale-up process could be successfully achieved if a scale-up criterion of constant oxygen mass transfer coefficient (k_La) is applied to the 50 L pilot-scale bioreactor system.

• Korean Chemical Engineering Research
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• v.48 no.5
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• pp.609-614
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• 2010

In this study, emulsion polymerizations for synthesizing acrylic pressure-sensitive adhesive(PSA) were carried out using 2-ethylhexyl acrylate(2-EHA), n-butyl acrylate(n-BA), methyl metacrylate(MMA) as fundamental monomers and acrylic acid(AAc) as a functional monomer in the presence of anionic SLS (sodium lauryl sulfate). To obtain the optimized synthetic condition in the polymerization, we analyzed the polymerization variables such as the effect of surfactant concentration and hydrophilic lipophilic values(HLB). At the same time, the final adhesive properties were also analyzed by the function of the initiator concentration and buffer concentration. In the results, the most stable emulsion was obtained at the surfactant concentrations between 3 and 5 wt%. It was also determined the effect of HLB value of nonionic surfactant and the initiator concentrations on the gel content. Stable emulsion is obtained using the surfactant having HLB value of 12.3. The rate of emulsion polymerization was increased at the initiator concentration greater than 1 wt%, but the stability of the emulsion was decreased. Finally, the effect of the buffer concentrations on the pH and the conversion of the acrylic emulsion product were experimentally measured. At the sodium bicarbonate concentration above 0.4 wt%, the buffer infulence was apparent. The buffer effect was fully acceptable at the concentrations between 0.6 and 0.8 wt% regardless of the monomer composition.

• Journal of Digital Convergence
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• v.10 no.8
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• pp.201-210
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• 2012

Because sensing odor varies depending on each person, even if the odor is released in line with the legal emission permission concentration levels, it can still become a social issue if a civil complaint is made. The purpose of this research is to study the possibility of putting Mn-Cu metallic oxide catalysts into practical use to economically eliminate acetaldehyde which produces a odor in the industrial process. An optimal operating parameter to eliminate acetaldehyde was deduced through a performance evaluation in the research laboratory and the performance was verified by applying the parameter into an actual facility as an on-the-site experiment through a Scale-up of pilot size. The operating temperature of the metallic oxide catalysts researched so far was at the minimum close to $220^{\circ}C$, and the $220^{\circ}C$ elimination efficiency was 50% or below. However, having experimented by using a Mn-Cu metallic oxide catalyst in this research, optimum elimination efficiency showed when space velocity (GHSV) was equal to or below 6,000 $hr^{-1}$. The average elimination efficiency was 61.2% when the catalyst controlling temperature was $120^{\circ}C$, 93.3% when the catalyst controlling temperature was $160^{\circ}C$, and 94.9% when catalyst controlling temperature was $180^{\circ}C$, thereby reflecting high elimination efficiency. The specific surface area of the catalyst was $200m^2/g$ before use, however, was reduced to $47.162m^2/g$ after 24 months and therefore showed that despite the decrease in specific surface area as time passed, there was no significant influence on the performance. Having operated Mn-Cu metallic oxide catalyst systems for at least two years on a site where there was no inflow of toxins like sulfur compounds and acidic gases, we were able to confirm that elimination efficiency of at least 90% was maintained.

• Korean Journal of Microbiology
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• v.46 no.1
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• pp.68-72
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• 2010

The ${\beta}$-1,4-glucanase gene of Bacillus licheniformis B1 was expressed in Esherichia coli BL21, and a protein with a mass of 50 kDa that was soluble was overproduced. A protein with a mass of 37 kDa was secreted from B. licheniformis. It seems that the ${\beta}$-1,4-glucanase produced in E. coli contained the leader peptide and unprocessed carboxy-terminal region, but its processing occurred in the carboxyterminal in Bacillus. The optimal temperature of ${\beta}$-1,4-glucanase was $40^{\circ}C$. The enzyme still had 76% maximal activity at $60^{\circ}C$. The optimal pH of the enzyme was 7. The enzyme retained considerable activities over the weak-acidic, neutral, and weak-basic pH range. Acidic fungal cellulases are used in food, detergent, pulp, paper, textile industries. However, studies about neutral and alkaline cellulase are not enough. The cellulase developed in this study may be useful for industrial applications in the fields of biofuel development.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.10
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• pp.1676-1684
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• 2004

Gel properties of recovered protein from mackerel, frozen blackspotted croaker, chicken breast and pork leg using acidic and alkaline processing were evaluated. Myofibrillar protein from mackerel by acidic processing did not form a heat-induced gel. However, the recovered protein including sarcoplasmic protein formed heatinduced gel. Breaking force of gel from mackerel processed at pH 10.5 was the lowest. A deformation value of frozen blackspotted croaker was the highest, followed by chicken breast, pork leg and mackerel. Whiteness of frozen blackspotted croaker was the highest among heat-induced gel. Breaking force, deformation and whiteness were decreased by addition of recovered protein from mackerel, but price was increased. A breaking force and whiteness of heat-induced gel added recovered protein from chicken breast were increased, and the price was greatly decreased. When the constraint of breaking force, deformation and price of raw material were set up above 110 g, 4.5 mm and below 2,000 won/kg. A optimum formulation for blending protein was 36∼50% for frozen blackspotted croaker, 34∼40% for chicken breast, 14∼25% for pork leg. The heat-induced gel of recovered protein from frozen blackspotted croaker showed compact structure compared to that of recovered protein from mackerel. A formulation of chicken breast and pork leg based on blackspotted croaker can be used in surimi based seafood products having various texture.

• Journal of the Korean Society of Clothing and Textiles
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• v.33 no.12
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• pp.1971-1978
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• 2009

This study investigated the adsorption of safflower yellow dye on wool protein fiber and the optimum dyeing conditions to test color reproducibility. In addition, the effects of mordants on dye adsorption, color, fastness, and photofading rate were also studied. The prepared dye in powder form was characterized with UV-vis spectroscopy and FT-IR spectrometric analysis. The color of dyed fabrics was characterized by CIE $L^*a^*b^*$ coordinates, H V/C, and K/S values. The color reproducibility of the dyed wool fabrics was examined. The amount of dye adsorption increased and also, the shade of the dyed wool fabrics became deeper and more saturated with increasing temperature, time, and dye concentration. The maximum color strength was obtained at pH 3.0. The shade of dyed wool fabrics ranged from light yellow to dark mustard yellow as the pH of the dye bath shifted from alkaline to acidic. Color reproducibility was reliable with color differences in the range of 0.53~1.75. Fastness to dry cleaning was relatively good at 4/5 rating irrespective of mordanting. Fe and Cu mordants showed the least color change of the dyed wool fabrics after exposure to light. Mordants did not contribute to improve dye uptake and color fastness, although they made variations in color tone. Safflower yellow dye can be used satisfactorily without mordants and will not cause damage to the environment.