• Journal of Life Science
• /
• v.18 no.8
• /
• pp.1159-1163
• /
• 2008

The purpose of this study was to establish a high-throughput assay method capable of estimating specific lipase activity at oil-water interface. The method is based on the fact that fluorescence intensity of fluorescein is affected by pH. The pH-dependence might be used to monitor pH change caused by the release of fatty acid through the action of lipase. Assay was performed by incubating a reaction mixture containing oil emulsion, fluorescein and enzyme and by monitoring fluorescence intensity periodically. Fluorescence intensity decreased linearly with a rate proportional to the enzyme amount. Linear relationship was observed between enzyme amount and reaction rate which was calculated from a graph of fluorescence change against time. The assay was possible at different pH conditions in the range of pH 6.0-8.0.

• Journal of Life Science
• /
• v.14 no.4
• /
• pp.627-635
• /
• 2004

In order to distinguish the genetic polymorphism among the olive flounder (Paralichthys olivaceus) obtained from East sea of Jumunjin, aquaculture of Tongyoung and Geoje, and East sea of North Korea, the ND-4 and cytochrome b genes of olive flounder were divided into 5 regions. Each region was analyzed by degenerating gel electrophoresis scanning (DGES), and by subsequent DNA sequencing. The DGES disclosed characteristic DNA polymorphisms in ND-4-2 and ND-4-3 regions of olive flounder, which were also confirmed by the DNA sequencing. The olive flounders obtained from the different marine areas showed DNA mutations in ND-4-2 region (G390A, C402T, and A411G； GenBank： AB028664), and also showed frequent DNA mutations in ND-4-3 region (C515G, C537T, C538T, A567G, G714A, C736T, G756A, A759T, T817C, and T829G), white the cytochrome b gene showed no DNA mutation both in the DGES and DNA sequencing. These data suggest that the ND-4-2 and ND-4-3 regions are candidate loci to distinguish the origin of olive flounder, and that the DGES used in this study provided fast and reliable informations for the genetic polymorphism.

• Journal of Elementary Mathematics Education in Korea
• /
• v.15 no.1
• /
• pp.77-93
• /
• 2011

Jisuguimundo(地數龜文圖) is a magic hexagon created by Suk-Jung Choi in his book about three hundreds years ago in Korea. Recently attention is focused on jisuguimundo, and it is known that jisuguimundos exist when magic number is from 77 to 108, however a general method making jisuguimundos is not known so far. Up to now, methods of making jisuguimundos using computers are known. In this study, a method making jisuguimundos is suggested using pairs of two numbers with sum p and q ($p{\neq}q$) alternately when magic number is from 88 to 92, and from 94 to 98, without using computer in elementary math class as a task for problem solving. Mathematical theory is introduced for this method, and jisuguimundos are presented which are found out through this method. Elementary students are expected to make their own jisuguimundo using this method.

• Journal of Life Science
• /
• v.18 no.3
• /
• pp.298-303
• /
• 2008

Using a phage peptide library approach, we have isolated a peptide ligand that binds to transferrin receptor on the surface of human melanoma cell, B16F10. The library was first screened twice by recovering internalized phages and was further screened three times by competitively eluting transferrin receptor-specific phages with human transferrin among the phages bound to the cell surface. The peptides displayed by the selected phages were fused to translocation and catalytic domain of Pseudomonas exotoxin to prepare recombinant toxins. After estimating cytotoxicity of each recombinant toxin toward B16F10 cell, seven clones were selected. Sequence analysis revealed that one of the clones displayed a peptide which had a significant sequence homology with human transferrin. The peptide was chemically synthesized and was shown to be functional in delivering cytotoxic agents into B16F10 cell via interaction with transferrin receptor.

• Journal of Life Science
• /
• v.16 no.1
• /
• pp.88-94
• /
• 2006

The random amplified polymorphic DNA (RAPD) technique was investigated as a potential tool for the origin identification of olive flounder (Paralichthys olivaceus) and redlip croaker (Pseudosciaena polyactis). Olive flounder specimens were collected from North Korea and several locations of South Korea (Jumunjin, Tongyoung and Geoje). Fishes obtained from Tongyeong and Geoje were cultured products. Redlip croaker specimens were collected from South Korea and China. Consistent and distinct diagnostic bands were easily identified in the RAPD patterns of the olive flounder specimens. Although consistent diagnostic bands rarely appeared in the RAPD pattern of redlip croaker specimens because of their genetic heterogeneity, we were able to find potential diagnostic bands in the average RAPD pattern of each origin.

• Journal for History of Mathematics
• /
• v.19 no.2
• /
• pp.1-24
• /
• 2006

We study the theory of finite series in Chosun Dynasty Mathematics. We divide it into two parts by the publication of Lee Sang Hyuk(李尙爀, 1810-?)'s Ik San(翼算, 1868) and then investigate their history. The first part is examined by Gyung Sun Jing(慶善徵, 1616-?)'s Muk Sa Jib San Bub(默思集算法), Choi Suk Jung(崔錫鼎)'s Gu Su Ryak(九數略), Hong Jung Ha(洪正夏)'s Gu Il Jib(九一集), Cho Tae Gu(趙泰耉)'s Ju Su Gwan Gyun(籌書管見), Hwang Yun Suk(黃胤錫)'s San Hak Ib Mun(算學入門), Bae Sang Sul(裵相設)'s Su Gye Soe Rok and Nam Byung Gil(南秉吉), 1820-1869)'s San Hak Jung Ei(算學正義, 1867), and then conclude that the theory of finite series in the period is rather stable. Lee Sang Hyuk obtained the most creative results on the theory in his Ik San if not in whole mathematics in Chosun Dynasty. He introduced a new problem of truncated series(截積). By a new method, called the partition method(分積法), he completely solved the problem and further obtained the complete structure of finite series.

• Journal of the Korean School Mathematics Society
• /
• v.14 no.3
• /
• pp.261-275
• /
• 2011

Seok-Jung Choi's Jisuguimundo mentioned as a brilliant legacy in the history of Korean mathematics had been cloaked in mystery for 300 years. In the meantime there has been some efforts to find solutions, and some particular answers were found, but no one achieved full success mathematically. By the way, H-alternating method showed that to find solutions of Jisuguimundo is possible, even though that method restricted magic number to 88~92 and 94~98. In this paper, $n{\times}n$ Jisuguimundo is defined, and it is showed that finding solutions of it is always possible in case of partition $({\upsilon}+1)+{\upsilon}+({\upsilon}+1)$ & co-partition ${\upsilon}+({\upsilon}+1)+{\upsilon}$, partition $({\upsilon}+1)+({\upsilon}-1)+({\upsilon}+1)$ & co-partition $({\upsilon}-1)+({\upsilon}+1)+({\upsilon}-1)$, partition $({\upsilon}+1)+({\upsilon}+2)+({\upsilon}+1)$ & co-partition $({\upsilon}+2)+({\upsilon}+1)+({\upsilon}+2)$, and partition $({\upsilon}+1)+({\upsilon}+3)+({\upsilon}+1)$ & co-partition $({\upsilon}+3)+({\upsilon}+1)+({\upsilon}+3)$. And It is suggested to find solutions of $n{\times}n$ Jisuguimundo could be used as a task for problem solving.

• (The)Study of the Eastern Classic
• /
• no.73
• /
• pp.9-33
• /
• 2018

This paper examines the literary theory of Gollyun(昆侖) Choe, Changdae(崔昌大, 1669-1720). He has authored a variety of works, and his works have been recognized in many literatures. Because of this, studying his literature is a meaningful. The theory of poem creation is as follows. It is the basic poem creationism that expresses the feelings that I experienced and felt as it is. The utility is to multiply and control the artist's feelings through his poem. However, the creative theory of being the best writer is different from this. It must be premised on finding from the heart and studying various books. If these qualities was provided, the words are clear and the meaning is condensed. He distinguished between general works and the best works, and presented their own creative theory and discussed their utility. The theory of prose utility is as follows. He emphasizes the importance of communicating with contemporaries and establishing important things of the day and making them easier to understand, without specifying the morality. This is a thoroughly realistic utility theory. In the classical chinese prose's history, 'Sadal(辭達)' and 'Susa(修辭)' were issues. He transcends the recognition of 'Sadal(辭達)' and 'Susa(修辭)' as zero-sum. In addition, he gives priority to the meaning of the writer and emphasizes self-realization, which is in common with other political soron(少論) writers' theories. When creating prose, simplicity and bizarreness were issues. He emphasizes concise writing. However, it can be realized when a writer with high opinion is aware of the reason and raises the core. Through various sources, he has completely rejected Ming(明) dynasty's former and latter seven master(前後七子). However, he did not exclude their work unilaterally, and recognized the work of Chin-Han dynasty(秦漢) and Dang-Song dynasty(唐宋). This is the same as his father Choiseokjung(崔錫鼎). He recognized Chin-Han dynasty(秦漢) and Dang-Song dynasty(唐宋) equally, and sought a simplified and summarized style.