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http://dx.doi.org/10.5352/JLS.2006.16.1.088

Random Amplified Polymorphic DNA Analysis for Origin Identification of Olive Flounder (Paralichthys olivaceus) and Redlip Croaker (Pseudosciaena polyactis)  

Kang Duk-Jin (Department of Chemistry and East Coastal Marine Bioresources Research Center)
Lee Suk-Keun (Department of Oral Pathology, Kangnung National University)
Jin Deuk-Hee (Faculty of Marine Bioscience & Technology, Kangnung National University)
Choi Suk-Jung (Department of Chemistry and East Coastal Marine Bioresources Research Center)
Publication Information
Journal of Life Science / v.16, no.1, 2006 , pp. 88-94 More about this Journal
Abstract
The random amplified polymorphic DNA (RAPD) technique was investigated as a potential tool for the origin identification of olive flounder (Paralichthys olivaceus) and redlip croaker (Pseudosciaena polyactis). Olive flounder specimens were collected from North Korea and several locations of South Korea (Jumunjin, Tongyoung and Geoje). Fishes obtained from Tongyeong and Geoje were cultured products. Redlip croaker specimens were collected from South Korea and China. Consistent and distinct diagnostic bands were easily identified in the RAPD patterns of the olive flounder specimens. Although consistent diagnostic bands rarely appeared in the RAPD pattern of redlip croaker specimens because of their genetic heterogeneity, we were able to find potential diagnostic bands in the average RAPD pattern of each origin.
Keywords
Olive flounder (Paralichthys olivaceus); redlip croaker (Pseudosciaena polyactis),; (randomamplified polymorphic DNA) RAPD; origin identification;
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1 Williams, D. J., S. Kazianis and R. B. Walter. 1998. Use of random amplified polymorphic DNA (RAPD) for identification of largemouth bass subspecies and their intergrades. Transactions of the American Fisheries Society 127, 825-832   DOI
2 Diakou, A. and C. I. Dovas. 2001. Optimization of random-amplified polymorphic DNA producing amplicons up to 8500 bp and revealing intraspecies polymorphism in Leishmania infantum isolates. Anal. Biochem. 288, 195-200   DOI   ScienceOn
3 Dinesh, K. R., T. M. Lim, K. L. Chua, W. K. Chan and V. P. E. Phang. 1993. RAPD analysis: an efficient method of DNA fingerprinting in fishes. Zoolog. Sci. 10, 849-854
4 Gomes, C., R. B. Dales and H. A. Oxendorf. 1998. The amplification of RAPD markers in stock discrimination of the four-wing flyingfish, Hirundichthys affinis in the central western Atlantic. Mol. Ecol. 7, 1029-1039   DOI   ScienceOn
5 Martinez, I. and L. A. Pastene. 1999. RAPD-typing of central and eastern north Atlantic and western north Pacific minke whales, Balaenoptera acutorostrata. ICES J. Marine Sciences 56, 640-651   DOI   ScienceOn
6 Meyer, R., Hofelein, C., Lüthy, J. and Candrian, U. 1995. Polymerase chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food. J. AOAC Int. 78, 1542-1551
7 Toledo, A., J. Martin-Sanchez, B. Pesson, C. Sanchiz-Marin and F. Morillas-Marquez. 2002 Genetic variability within the species Leishmania infantum by RAPD. A lack of correlation with zymodeme structure. Mol. Biochem. Parasitol. 119, 257-264   DOI   ScienceOn
8 Welsh, J. and M. McClelland. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18, 7213-7218   DOI
9 Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey. 1990, DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535   DOI
10 Barman, H. K., A. Barat, B. M. Yadav, S. Banerjee, P. K. Meher, P. V. G. K. Reddy and R. K. Jana. 2003. Genetic variation between four species of Indian major carps as revealed by random amplified polymorphic DNA assay. Aquaculture 217, 115-123   DOI   ScienceOn
11 Bartlett, S. E. and W. S. Davidson. 1991. Identification of Thunnus tuna species by polymerase chain reaction and direct sequence analysis of their mitochondrial cytochrome b genes. Can. J. Fish. Aquat. Sci. 48, 309-317   DOI
12 Cadez, N., P. Raspor, A. W. de Cock, T. Boekhout and M. T. Smith. 2002 Molecular identification and genetic diversity within species of the genera Hanseniaspora and Kloeckera. FEMS Yeast Res. 1, 279-289
13 Callejas, C. and M. D. Ochando. 1998. Identification of Spanish barbel species using the RAPD technique. J. Fish Biol. 53, 208-215   DOI
14 Patterson, R. L. S. and S. J. Jones. 1990. Review of current techniques for the verification of the species origin of meat. Analyst 115, 501-506   DOI
15 Naish, K. A., M. Warren, F. Bardakci, D. O. F. Skibinski, G. R. Carvalho and G. C. Mair. 1995. Multilocus DNA fingerprinting and RAPD reveal similar genetic relationships between strains of Oreochromis niloticus (Pisces, Cichlidae). Mol. Ecol. 4, 271-274   DOI   ScienceOn
16 Callejas, C. and M. D. Ochando. 2001. Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the Iberian Peninsula. J. Fish Biol. 59, 1589-1599   DOI
17 Bardakci, F. and D. O. F. Skibinski. 1994. Application of the RAPD technique in tilapia fish: Species and subspecies identification. Heredity 73, 117-123   DOI   ScienceOn
18 van de Lest, C. H. A., J. H. Veerkamp and T. H. van Kuppevelt. 1995. ImageCalc: a Microsoft Windows application for quantitative image analysis and comparison. Biotechniques 18, 1050-1055
19 Fraga, J, L. Rojas, I. Sariego and C. A. Sarria. 2002 Optimization of random amplified polymorphic DNA techniques for its use in genetic studies of Trichomonas vaginalis isolates. Infect. Genet. Evol. 2, 73-75   DOI   ScienceOn
20 Bielawski, J. P. and D. E. Pumo. 1997. Random amplified polymorphic DNA (RAPD) analysis of Atlantic Coast striped bass. Heredity 78, 32-40   DOI
21 Krawczyk, B., L. Naumiuk, K. Lewandowski, A. Baraniak, M. Gniadkowski, A. Samet and J. Kur. 2003. Evaluation and comparison of random amplification of polymorphic DNA, pulsed-field gel electrophoresis and ADSRRS-finger-finger-printing for typing Serratia marcescens outbreaks. FEMS Immunol. Med. Microbiol. 38, 241-248   DOI   ScienceOn