• Analytical Science and Technology
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• v.17 no.6
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• pp.481-488
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• 2004

Solvent extraction of Ni(II) into a chloroform by using salen[N,N'-Bis (salicylidene)-ethylenediamine] as a ligand has been studied. Salen was synthesized from ethylenediamine and salicylaldehyde by simple condensation reaction in an ethanol. Salen formed a 1 : 1 complex with Ni(II) and its extraction constant was $10^{5.12}$. For the determination of Ni(II) in sea water samples, some experimental conditions such as pH of solution, amount of salen, acid type and concentration for back extraction, extraction time, and influence of foreign ions were optimized by using a synthetic sea water. The sea water of which the composition was similar to a natural sea water was synthesized in this laboratory. Trace Ni(II) was extracted into the chloroform in the weak basic solution above pH 8. And the nickel could be quantitatively extracted with the concentration of salen higher than $1.2{\times}10^{-4}mol/L$. This concentration was more than 180 times of Ni(II) in the solution with a mole ratio. Real samples of Korean coastal sea water were analyzed under optimized conditions. Even though Ni(II) was not detected in these samples. Recoveries more than 98% were obtained in the samples which 40 ng/mL of Ni(II) was spiked. And detection limit of proposed method was 1.3 ng/mL. From these results, it could be known that salen of this type would be applied for the determination of trace metals as an organic chelating reagent.

• Applied Biological Chemistry
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• v.39 no.5
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• pp.349-354
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• 1996

The Polyvinyl alcohol(PVA) oxidase involved in PVA degradation by microorganism has been purified to homogeneity from culture broth of Xanthomonas campestris J2Y grown in a minimal medium containing PVA as a sole carbon source. The enzyme was purified by DEAE-cellulose chromatograpy and Sephadex G-150 gel filtration. The purified PVA oxidase was electrophoretically homogeneous both in the absence and presence of SDS. The molecular weight of the enzyme was estimated to be about 55,000 daltons by SDS-polyacrylamide gel electrophoresis and Sephadex G-150 gel filtration. The native enzyme existed as a monomer. The optimal pH and temperature was shown to be pH 7 and $37^{\circ}C$ respectively. The activity of enzyme was stable below $55^{\circ}C$ and between pH range of $5{\sim}11$. The enzyme activity was significantly inhibited by metal compounds such as $Ag^{2+},\;Hg^{2+}$. While, metal ions such as $Mn^{2+},\;and\;Cu^{2+}$ stimulated the reaction. Km value of the enzyme for PVA was $7.04{\times}10^{-2}mmol/{\ell}$.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2007.11a
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• pp.229-229
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• 2007

나노결정 합금재료를 전력선 통신 커플러용 자심재료로 응용하기 위해서는 고주파 대역에서의 손실 특성이 제어되어야 한다. 즉 고속 전력선 통신을 위한 자심재료의 투자율 및 완화 주파수 등의 전자기적 특성은 30MHz까지 우수하고 안정적으로 유지되어야 하며, 높은 투자율 및 자속밀도, 공진주파수뿐만 아니라 낮은 전력손실 값을 가져야 한다. 따라서 본 연구에서는 나노결점 합금 리본 표면에 딥 코팅, 졸-겔법, 진공함침 등의 방법을 이용하여 PZT, $TiO_2$ 및 $SiO_2$ 등의 산화물 고저항층을 형성시켜 자기적 성질을 유지하면서 고주파 대역의 와전류 손실을 감소시켜 통신용 자심재료로의 응용성을 향상시키고자 하였다. PZT 슬러리의 제타전위 조절을 통해 최적의 분산조건을 얻을 수 있었고, 평균 150nm인 PZT 입자의 초미립자와 가소제, 분산제, 결합제의 첨가조건을 확립할 수 있었다. 딥-코팅은 슬러리 내 유지시간 10초, 인상속도 5mm/min로 30회 반복되었을 때 가정 우수한 특성을 나타내었으며, 고주파 대역에서의 손실 감소효과를 나타내었다. 그리고 졸-겔법에 의해 제조된 슬러리를 이용한 $TiO_2$와 $SiO_2$ 산화물 저항층 코팅을 통해 금속 알콕사이드의 혼합조건 및 저항층 형성용 슬러리의 제조조건을 확립하였고, 합금 리본표면에 균일하고 우수한 점착력을 가지는 저항층을 형성시킬 수 있었으며, 이에 따른 코어손실의 감소효과를 나타낼 수 있었다. 또한 진공 함침법을 통한 저항층 형성에서, $TiO_2$ 나노분말을 표면 저항층으로 코팅했을 때, 가장 높은 코어손실 감소효과를 나타내었다. 한편, 표면 저항층이 형성된 나노결정 합금으로 제조한 자심재료를 이용하여 전력선 통신용 비접촉식 커플러에의 적용과 시험을 통해 고주파 손실 감소효과에 의한 신호전송 특성과 전류특성을 향상시킬 수 있었다.

• The Journal of Engineering Research
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• v.6 no.2
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• pp.23-32
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• 2004

Metals, ceramics and polymers are widely used as bioimplant materials. However, Ti and Ti alloys are widely used because of its high strength to weight ratio and good biocompatibility when implanted in the body. In this experiment, Ti alloys of Grade-4 (gr4), 0.2 wt % Fe, 0.5 wt % Fe and 2 wt % Fe were studied for their surface morphology and HAp forming ability on the metal substrate for different treatments. Intially, the samples were mechanically polished on silicone carbide paper (No.-2000). The polished samples were treated with 5M NaOH solution at $60^{\circ}C$ for 24 hours. The NaOH treated samples were washed gently with distill water and dried at $40^{\circ}C$ for 1 day. The dried samples were heated in air at $600^{\circ}C$ for 1 hour. The surface morphology of these samples were studied using SEM. The SEM studies showed network of pores in all samples. These samples were immersed in stimulated body fluids (SBF) kept at $36.5^{\circ}C$ for different periods over the length of 1 to 14 days. The apatite formation was confirmed on all Ti-alloys using EDAX.

• Korean Journal of Food Science and Technology
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• v.32 no.5
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• pp.1158-1167
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• 2000

To produce ${\beta}-cyclodextrin({\beta}-CD)$, a cyclodextrin glucanotransferase(CGTase) producing Aspergillus sp. CC-2-1 was isolated from soil. The enzyme was purified and its enzymological characteristics were investigated. It was found that production of CGTase reached to the maximum when the wheat bran medium containing 0.1% albumin, 2% $(NH_4)_2S_2O_8$, 2% soluble starch and 0.2% $KH_2PO_4$ was cultured for 5 days at $37^{\circ}C$. The purity of CGTase was increased by 13.14 folds after DEAE-cellulose ion exchange chromatography and Sephadex G-100, G-150 gel filtration and the specific activity was 172.14 unit/mg. Purified enzyme was confirmed as a single band by the polyacrylamide gel electrophoresis. The molecular weight of CGTase was estimated to be 27,800 by Sephadex G-100 gel filtration and SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature for the CGTase activity were 9.0 and $80^{\circ}C$, respectively. The enzyme was stable in pH $8.0{\sim}11.0$ at $60{\sim}80^{\circ}C$. The activity of purified enzyme was activated by $K^+,\;Cu^{2+}$ and $Zn^{2+}$. The activity of the CGTase was inhibited by the treatment with 2,4-dinitrophenol and iodine. The result suggests that the purified enzyme has phenolic hydroxyl group of tyrosine, histidine imidazole group and terminal amino group at active site. The reaction of this enzyme followed typical Michaelis-Menten kinetics with the $K_m$ value of 18.182 g/L with the $V_{max}$ of 188.68 ${\mu}mole/min$. The activation energy for the CGTase was calculated by Arrhenius equation was 1.548 kcal/mol.

• Current Research on Agriculture and Life Sciences
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• v.14
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• pp.111-122
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• 1996

S. chibaensis J-59 produced an extracellular xylanase in a CSL medium composed of 1.5% com steep liquor, 0.1% $MgSO_4{\cdot}7H_2O$, 0.012% $CoCl_2{\cdot}6H_2O$, and 0.15% glucose containing xylan. but it did not produce in the culture medium containing xylose. The production of enzyme reached to a maximum level (0.83 uints/ml) when bacteria were cultured in 2.5 l jar fermentor for 48hrs at $30^{\circ}C$ and pH 7.0. Furthermore, S. chibaensis J-59 produced an intracellular glucose isomerase in a medium containing xylan and/or xylose. Xylanase was purified 29-fold over the culture supernatants of S. chibaensis J-59 by ammonium sulfate fractionation, chromatography on DEAE-Sephadex A-50, and gel filtration on Sephadex G-200. The purified enzyme is a monomeric enzyme with a native molecular mass of 25 kDa and a subunit molecular mass of 25 kDa. The purified enzyme requires $Mg^{2+}$ for activity, $Ca^{2+}$, $Co^{2+}$ is not an inhibitor but inhibit by $Fe^{3+}$, $Hg^{2+}$, and $Cu^{2+}$, sodium dodecyl sulfate, N-bromosuccinide. Pattern of hydrolysis demonstrated that the xylanase was an endo-splitting enzyme able to break down birchwood xylan at random giving xylobiose, xylotriose and xylotetrose as the main end products.

• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.705-716
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• 2017

In this study, Indium-Titanium hydroxide particle with 0.5, 1.0, 1.5 wt% of $TiO_2$ were synthesized by sol process and adding the base, ITiO(Indium Titanium Oxide) particles were obtained by gelling at $200^{\circ}C$ and $500^{\circ}C$. The ITiO particle's size with gel process at $200^{\circ}C$ was smaller than ITiO particle's size with gel process $500^{\circ}C$. The ITiO particle with gel process at $200^{\circ}C$ was used to fabricate dense ITiO target. ITiO targets with 0.5, 1.0, 1.5 wt% of $TiO_2$ were fabricated and used to obtain ITiO thin films onto glass by sputtering. Among those sputtered ITiOs' thin films, ITiO thin film with 0.4 % of $O_2$ and 0.5 wt% of $TiO_2$ showed the lowest specific resistance, highest charge mobility and lowest carrier concentration. It was found the light transmittance of the ITiO film were increased highly compared to light transmittance of ITO (Indium Tin Oxide) thin film over Infrared wavelength ranges.

• Resources Recycling
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• v.27 no.4
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• pp.29-35
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• 2018

In this study, the electrochemical redox behavior of neodymium in non-aqueous electrolytes was investigated to confirm the possibility of neodymium metallurgy at room temperature. The non-aqueous electrolytes include ionic liquids such as $[C_4mim]PF_6$, $[C_4mim]Cl$, and $[P_{66614}]PF_6$, ethanol which are highly soluble in neodymium salts, and mixed electrolytes based on carbonate with highly electrochemical stability. The electrochemical redox properties of neodymium were better than those of other electrolytes in the case of the mixed electrolyte based on ethylene carbonate (EC)/di-ethylene carbonate (DEC). Ethanol was added to improve the physical properties of the mixed electrolyte. Thorough the analysis about ionic conductivity of EC/DEC ratio, ethanol content and $NdCl_3$ concentration, the best electrolyte composition was 50 vol% content of ethanol and 0.5 M of $NdCl_3$. Using cyclic voltametry and linear sweep voltametry, a current peak estimated at -3.8 V (vs. Pt-QRE) was observed as a limiting current of neodymium reduction. Potentiostatic electrolysis for 18 hours at room temperature at -6 V (vs. Pt-QRE) confirmed that metallic neodymium was electrodeposited.

• Korean Chemical Engineering Research
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• v.57 no.4
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• pp.559-564
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• 2019

Hierarchical $ZnCo_2O_4$ hollow nanofibers were prepared by electrospinning and subsequent heat-treatment process. The spinning solution containing polystyrene (PS) nanobeads was electrospun to nanofibers. During heat-treatment process, PS nanobeads in the composite were decomposed and therefore generated numerous pores uniformly in the structure, which facilitated the heat transfer and gas penetration into the structure. The resulting hierarchical $ZnCo_2O_4$ hollow nanofibers were applied as an anode material for lithium-ion batteries. The discharge capacity of the nanofibers was $815mA\;h\;g^{-1}$ ($646mA\;h\;cm^{-3}$) after the 300th cycle at a high current density of $1.0A\;g^{-1}$. However, $ZnCo_2O_4$ nanopowders showed the discharge capacity of $487mA\;h\;g^{-1}$ ($450mA\;h\;cm^{-3}$) after 300th cycle. The excellent lithium ion storage property of the hierarchical $ZnCo_2O_4$ hollow nanofibers was attributed to the synergetic effects of the hollow nanofiber structure and the $ZnCo_2O_4$ nanocrystals composing the shell. The hierarchical hollow nanofiber structure introduced in this study can be extended to various metal oxides for various applications, including energy storage.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.17 no.2
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• pp.127-137
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• 2019

Zr electrorefining is demonstrated herein using Zirlo tubes in a chloride-fluoride mixed molten salt in the presence of $AlF_3$. Cyclic voltammetry reveals a monotonic shift in the onset of metal reduction kinetics towards positive potential and an increase in intensity of the additional peaks associated with Zr-Al alloy formation with increasing $AlF_3$ concentration. Unlike the galvanostatic deposition mode, a radial plate-type Zr growth is evident at the top surface of the salt during Zr electrorefining at a constant potential of -1.2 V. The diameter of the plate-type Zr deposit gradually increases with increasing $AlF_3$ concentration. Scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDX) and X-ray photoelectron spectroscopy (XPS) analyses for the plate-type Zr deposit show that trace amount of Al is incorporated as Zr-Al alloys with different chemical compositions between the top and bottom surface of the deposit. Addition of $AlF_3$ is effective in lowering the residual salt content in the deposit and in improving the current efficiency for Zr recovery.