• Korean Journal of Organic Agriculture
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• v.25 no.4
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• pp.901-916
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• 2017

This study was conducted to evaluate natural plant extracts for methane gas reduction in ruminants. Rumen fluid was collected from cannulated Hanwoo cow ($450{\pm}30kg$) consuming 400 g/kg concentrate and 600 g/kg timothy. The 15 ml of mixture comparing McDougall's buffer and rumen fluid in the ratio 2 to 1, was dispensed anaerobically into 50 ml serum bottles. Rumen fluid contents were collected and in vitro fermentation prepared control (timothy, 300 mg), ginseng, balloon flower, yucca plant, camellia, tea plant and ogapi extracts were added at the level of 5% against 300 mg of timothy as a substrate (v/w) and incubated for 3, 6, 9, 12, 24, 48, and 72 h. In vitro pH values range 6.55~7.41, this range include rumen titration. The dry matter digestibility was not differ between all treatments and control. Total gas emission was significantly higher (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Carbon dioxide emission was not differ all treatments on 9 h than in control and significantly higher (p<0.05) yucca plant, camellia and tea plant treatments on 12 h than control. Methane emission was not differ all treatments on 6 h than in control. The rumen microbial growth rate was significantly higher (p<0.05) in ginseng, balloon flower on 12 h and significantly higher (p<0.05) in ginseng, yucca plant, tea plant and ogapi treatments on 24 h than in control. Total VFA was significantly higher (p<0.05) in tea plant and ogapi treatments on 12 h than in control and significantly higher (p<0.05) in ginseng, balloon flower treatments on 48 h than in control. Acetic acid was significantly lower (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Propionic acid was significantly higher (p<0.05) in ginseng and balloon flower treatments on 48 h than in control. As a results, sixth natural plant extracts had no significant effect dry matter digestibility and negative on rumen fermentation, but not effect methane reduction.

• Journal of Bio-Environment Control
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• v.31 no.2
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• pp.133-141
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• 2022

Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L^-1 of BAP and 2.0 mg·L^-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L^-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L^-1 of banana and 20 g·L^-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L^-1 of coconut water) and plant growth regulators (2.0 mg·L^-1 of BAP and 1.0 mg·L^-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.

• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.467-478
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• 2006

Maintenance of an optimal air quality in the enclosed pig building is potentially important in terms of pig performance and farmer health. The objective of this on-site experiment is to evaluate and compare efficiencies of currently utilized biological additives to reduce odor emissions from the enclosed pig building. As a result, generally all the additives except for salt water, artificial spice and essential oil were proved ineffective in reducing odor generation. The beneficial effects of salt water, artificial spice and essential oil on odor reduction were highlighted on ammonia, odor intensity and offensiveness, and sulfuric odorous compounds, respectively. To efficiently utilize odor masking agent such as the artificial spice, ventilation rate should keep slightly lower than the optimal level. Essential oil functioned well as not only masking agent but also antimicrobial agent for reducing odor. To precisely quantify odor concentration, it should be measured by not the odor sensor but the olfactometry technique.

• Korean Journal of Food Science and Technology
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• v.42 no.1
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• pp.63-68
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• 2010

The use of deodorizing plant products such as persimmon leaves, pine needles and fennel seeds to mask the characteristic odor of kimchi has been proposed. Therefore, in this study, the effect of deodorizing plants on kimchi fermentation and the profile of volatile flavor compounds was investigated. During sensory evaluation tests of sour and fermentation odors of kimchi, plant extract-added kimchi showed higher sensory scores than chopped plant-added kimchi. Additionally, kimchi containing pine needle extracts (0.3-0.6%) and fennel seed extracts (0.05-0.1%) showed higher sensory scores than untreated kimchi. In addition, pine needle extract and fennel seed extract had a significant influence on kimchi fermentation characteristics such as pH, total acidity and the concentration of lactic acid bacteria. The volatile flavor compounds of kimchi and deodorizing plant extracts were also analyzed using GC/MS and the correlation of volatile flavor compounds in kimchi, plant extracts and plant-added kimchi was examined. The results revealed that the sour and fermentation odors of kimchi were reduced using deodorizing plant extracts by changing the kimchi fermentation characteristics.

• Korean Journal of Plant Resources
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• v.24 no.1
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• pp.105-112
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• 2011

The objective of this study was to determine the effect of antioxidant enzyme activity and radical scavenging activities of thirteen kinds of natural plant pigments. The analytic method of antioxidant activities were measured by estimating DPPH free radical scavenging and nitrite scavenging ability activity. The free radical scavenging activity by method using stable free radical DPPH was the highest in the red cabbaged pigment. Addition of ethanol extract 1mg/ml from onion peel pigment displayed remarkable effect on nitrite scavenging ability about 91.9%. Antioxidative enzyme activity was evaluated in terms of superoxide dismutase(SOD), catalase(CAT), and ascorbate peroxidase(APX) activity. The bitter melon pigment had the highest SOD activity of 87.3%. The activities of CAT and APX were higher in the mulberry leave pigment compared with other natural plant pigments. In contrast, CAT activity of plant pigment samples were unaffected. These results suggest that natural plant pigment had the potent biological activities such as antioxidant enzyme activities, and that their activities exhibited differently depending on each kind of pigments.

• Journal of the Korea Convergence Society
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• v.8 no.12
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• pp.109-117
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• 2017

The purpose of the study aimed to explore the possibility for natural preservative convergence materials by examining the antioxidant activity and antimicrobial effect of 19 wild plants in Jiri Mountain. Total polyphenols were higher in the order of CS(Camellia sinensis L., 87.9 mg GAE/ g), MP(Mentha piperascens Holmes., 85.1 mg), NN(Nelumbo nucifera Gaertn., 65.0 mg) and PD(Pinus densiflora Siebold & Zucc., 52.8 mg). Total flavonoids were high in NN(25.7 mg QUE / g) and MP (25.4 mg QUE / g). CS(58.1%), NN(47.9%), and MP(40.6%) showed high ABTS radical scavenging ability and the result was similar in DPPH radical scavenging ability. The extracts of HC(Hemerocallis coreana Nakai.), PD, and CO(Cornus officinalis Siebold et Zucc.) showed the highest inhibitory effect on the growth of E. coli. The extracts of PK(Pulsatilla koreana Nakai ex Nakai.), SC(Saururus chinensis Baill.), and MC(Smilax china L.) completely inhibited the proliferation of S. aureus, showing the possibility to be developed as natural preservatives and disinfectants.

• The Journal of Natural Sciences
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• v.18 no.1
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• pp.39-46
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• 2007

Three kinds of plant fermentation broths and one liquor were prepared using persimmon, Ulmus davidiana var. japonica and barley, and investigated its physiological functionality. Anti-aging antioxidants activity was the highest of 99% in Ulmus dacidiana bar. japonica liquor and also was high in the persimmon vinegar(68.3 %). SOD-like activity show also 80.6% in the Ulmus davidiana var japonica liquor however the other fermentation broths were less 25%. Antihypertensive angiotensin I-converting enzyme inhibitory activity and fibrinolytic activity were weak or not detected in all the fermentation broths. Anti-dementia acetylcholinesterase inhibitory activity were showed 54.7% in the Ulmus davidiana var. japonica liquor. Our results showed that the Ulmus davidana var. japonica liquor has the potential to become a new functional liquor because of high antioxidant activity and anti-dementia activity.

• Korean Journal of Environmental Agriculture
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• v.32 no.4
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• pp.338-342
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• 2013

BACKGROUND: Two Field tests were conducted at Buan and Yangpyung in 2012 and 2013 to confirm controlling effect of mulberry popcorn disease and mulberry Sucker pests which are becoming serious in mass cultivation area of eco-friendly farming mulberry. METHODS AND RESULTS: As the treatments, 4 Natural products and 3 microbials were applied and 4 mulching materials were used. On the prevent effect of mulberry popcorn disease(Sclerotinia shiraiana) by using mulching materials, nonwoven fabric mulching showed worse effect than non-mulched treatment plot. Moreover, rice straw mulching showed significantly worse effect compare to nonwoven fabric and herb mulching treatments. Natural plant extracts and microbials showed 40~65% control value in 2013, which is little bit worse than 2012 results. On the control effect of mulberry Sucker(Amomoneura mori), organic products which combined with neem, sophora and derris showed excellent result as similar control level of Thiophanate-methyl. It means the chemical products can be replaced with organic product. CONCLUSION(S): According to the 2 years studied results, integrate eco-friendly farming measures are recommended for control of mulberry popcorn disease, because any single method is seemed not sufficient enough. However, natural plant extracts mixture is recommended as a product to control of mulberry Sucker.

• Food Science and Preservation
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• v.20 no.5
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• pp.597-601
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• 2013

In this study, we searched for bioactive compounds from natural resources with a supercritical extract. We selected the extracts of Chrysanthemum zawadskii, Lufa cylindrica, Paeonia lactiflora, Gardenia jasminoides and Scutellaria baicalensis, as natural materials, and evaluated the effects of their skin barrier function. We found that these extracts increased the transactivation activity of the PPAR-responsive element (PPRE) and the anti-oxidation with different priorities, respectively. In addition, these extracts promoted the expression of proteins related to cornified envelope (CE) formation, such as involucrin. From these results, we suggest that natural materials from supercritical extracts will be pertinent candidates for the improvement of the epidermal permeability barrier function.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.209-209
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• 1994

천연자원으로부터 얻어진 항암 또는 세포독성물질로는 주로 alkaloid, lignan, terpenoid, macrolide 등에 속하는 물질들이고 그 중 sesquiterpene lactone계열 화합물에서 세포독성 및 항종양 활성이 있는 물질이 다수 얻어졌으며 그 대부분은 국화과의 식물에서 분리 보고되고 있다. Achillea 속 식물은 국화과에 속하는 다년생초본으로 남한에는 톱풀과 산톱풀 2종만이 자생한다. screening 결과 이 두 식물에는 segquiterpene lactone 화합물과 Peroxide등 다수의 활성성분이 함유되어 있을 것으로 기대되어 본 연구에 착수하였다. 두 식물의 각 분획에 대하여 SNU-1(위암세포주)과 SNU-354(간암세포주)를 이용해 검색한 결과 두 식물 모두 MC ext.와 MeOH ext.에서 현저한 세포독성을 보였으며 현재 MC ext. 로부터 세포독성물질의 분리를 수행하고 있다. EtOAc ext.는 세포독성은 거의 나타내지 않았으나 flavonoid 성분이 다수 존재함을 확인하였으며 면역활성 및 enzyme inhibition등의 활성을 검토하고자 화합물을 분리하였다. 그 결과 톱풀로 부터는 luteolin-7-0-glucoside와 apigenin-7-0-glucoside를 그리고 산톱풀로부터는 luteolin-7-0-glucoside, apigenin-7-0-glucoside, luteolin, apigenin을 분리 확인 동정하였다.