Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Studies on Natural Plant Extracts for Methane Reduction in Ruminants

반추동물의 메탄감소를 위한 천연식물 추출물에 관한 연구

  • 이신자 (경상대학교 농업생명과학연구원&중점연구소) ;
  • 엄준식 (경상대학교 응용생명과학부(BK21)) ;
  • 이수경 (경상대학교 농업생명과학연구원) ;
  • 이일동 (경상대학교 응용생명과학부(BK21)) ;
  • 김현상 (경상대학교 응용생명과학부(BK21)) ;
  • 강한별 (경상대학교 응용생명과학부(BK21)) ;
  • 이성실 (경상대학교 응용생명과학부(BK21)&농업생명과학연구원)
  • Received : 2017.09.29
  • Accepted : 2017.10.24
  • Published : 2017.11.30
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Abstract

This study was conducted to evaluate natural plant extracts for methane gas reduction in ruminants. Rumen fluid was collected from cannulated Hanwoo cow ($450{\pm}30kg$) consuming 400 g/kg concentrate and 600 g/kg timothy. The 15 ml of mixture comparing McDougall's buffer and rumen fluid in the ratio 2 to 1, was dispensed anaerobically into 50 ml serum bottles. Rumen fluid contents were collected and in vitro fermentation prepared control (timothy, 300 mg), ginseng, balloon flower, yucca plant, camellia, tea plant and ogapi extracts were added at the level of 5% against 300 mg of timothy as a substrate (v/w) and incubated for 3, 6, 9, 12, 24, 48, and 72 h. In vitro pH values range 6.55~7.41, this range include rumen titration. The dry matter digestibility was not differ between all treatments and control. Total gas emission was significantly higher (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Carbon dioxide emission was not differ all treatments on 9 h than in control and significantly higher (p<0.05) yucca plant, camellia and tea plant treatments on 12 h than control. Methane emission was not differ all treatments on 6 h than in control. The rumen microbial growth rate was significantly higher (p<0.05) in ginseng, balloon flower on 12 h and significantly higher (p<0.05) in ginseng, yucca plant, tea plant and ogapi treatments on 24 h than in control. Total VFA was significantly higher (p<0.05) in tea plant and ogapi treatments on 12 h than in control and significantly higher (p<0.05) in ginseng, balloon flower treatments on 48 h than in control. Acetic acid was significantly lower (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Propionic acid was significantly higher (p<0.05) in ginseng and balloon flower treatments on 48 h than in control. As a results, sixth natural plant extracts had no significant effect dry matter digestibility and negative on rumen fermentation, but not effect methane reduction.

6가지 천연 식물 추출물의 첨가가 반추동물의 메탄 감소에 대한 연구를 수행하였다. 반추위액은 cannula가 장착된 한우에서 채취하였으며, 공시축의 사양관리는 timothy와 농후사료 6:4 비율로 급여하였다. 50 ml serum bottle에 timothy 0.3 g, 반추위액 5 ml, McDougall's buffer 10 ml를 각각 넣고 인삼, 도라지, 유카식물, 동백나무, 차나무, 오갈피 추출물을 기질의 5%를 첨가한 뒤 발효시간대별(3, 6, 9, 12, 24, 48, 및 72시간) 7처리 3반복 수행하였다. pH는 6.55~7.41로 반추위 적정 pH 범위에 속하였다. 건물소화율은 첨가구에서 대조구에 비해 유의적(P<0.05)인 차이가 없었다. 총 가스 발생량은 발효 24시간대 인삼, 도라지 첨가구에서 대조구에 비해 유의적(P<0.05)으로 증가하였다. 이산화탄소 발생량은 발효 9시간대, 메탄 발생량은 6시간대에서 첨가구와 대조구간 유의적(P<0.05)인 차이가 나타나지 않았다. 미생물성장량은 발효 12시간대 인삼, 도라지 첨가구에서 대조구에 비해 유의적(P<0.05)으로 증가하였고, 발효 24시간대 인삼, 유카식물, 차나무, 오갈피 첨가구에서 대조구에 비해 유의적(P<0.05)으로 증가하였다. Total VFA 농도는 발효 12시간 차나무, 오갈피 첨가구에서 대조구에 비해 유의적(P<0.05)으로 증가하였고, 발효 48시간 인삼, 도라지 첨가구에서 대조구에 비해 유의적(P<0.05)으로 증가하였다. Acetate 농도는 발효 24시간 인삼, 도라지 첨가구에서 대조구에 비해 유의적(P<0.05)으로 감소하였고, propionate 농도는 발효 48시간 인삼, 도라지 첨가구에서 대조구에 비해 유의적(P<0.05)으로 증가하였다. 결과적으로 6가지 천연식물 추출물의 첨가는 in vitro 반추위 발효성상에는 이상이 없었으나 메탄 감소의 효과는 나타나지 않았다. 추후 6가지 천연 식물 추출물의 농도를 달리하여 추가적인 시험이 필요할 것으로 사료 된다.

Keywords

References

  1. Ahn, K. S., E. J. Noh, H. L. Zhao, S. H. Jung, S. S. Kang, and Y. S. Kim. 2005. Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-${\kappa}B$ activation in RAW 264.7 cells. Life Sci. 76: 2315-2328. https://doi.org/10.1016/j.lfs.2004.10.042
  2. AOAC. 2012. Official methods of analysis 19th edition. Association of official analytical chemists, Washington, D. C. USA.
  3. Choi, C. Y., J. Y. Kim, Y. S. Kim, Y. C. Chung, J. K. Seo, and H. G. Jeong. 2001. Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factor-a from murine macrophages. Int. Immunopharmacol. 1: 1141-1151. https://doi.org/10.1016/S1567-5769(01)00047-9
  4. Crutzen P. 1995. The role of methane in atmospheric chemistry and climate. Proceedings of the eighth international symposium on ruminant physiology. 291-316.
  5. Duncan, D. B. 1995. Multiple range and multiple F test. Biometrics. 11: 1-6.
  6. Goel, G. and H. P. Makkar. 2012. Methane mitigation from ruminants using tannins and saponins. Trop. Anim. Health Prod. 44: 729-739. https://doi.org/10.1007/s11250-011-9966-2
  7. Grobner, M. A., D. E. Johnson, S. R. Goodall, and D. A. Benz. 1982. Sarsaponin effects on in vitro continuous flow fermentation of a high grain diet. J. Anim. Sci. 33: 64-66.
  8. Hahn, Y. S. 2005. Antimicrobial effects of Camellia japonica L. leaves extract on foodborne pathogenic microorganisms. Korean J. Food Sci. Technol. 37: 113-121.
  9. Hristov, A. N., T. A. McAllister, F. H. Van Herk, K. Cheng, C. J. Newbold, and P. R. Cheeke. 1999. Effect of yucca schidigera on ruminal fermentation and nutrient digestion in heifers. J. Anim. Sci. 77: 2554-2563. https://doi.org/10.2527/1999.7792554x
  10. Hwang, H. S., J. U. Ok, S. J. Lee, G. M. Chu, K. H. Kim, Y. K. Oh, S. S. Lee, and S. S. Lee. 2012. Effects of halogenated compounds on in vitro fermentation characteristics in the rumen and methane emissions. J. Life Sci. 22: 1187-1193. https://doi.org/10.5352/JLS.2012.22.9.1187
  11. Hwang, H. S., D. G. Ha, S. K. Lee, I. D. Lee, S. J. Lee, and S. S. Lee. 2013. Effects of terpenoids rich plant extracts on ruminal fermentation and methane production. Korean J. Organic Agric. 21: 629-646. https://doi.org/10.11625/KJOA.2013.21.4.629
  12. IPCC (Intergovernment Panel on Climate Change). 2001. The scientific basis. Cambridge, UK; Cambridge University Press.
  13. Johnson, K. A. and D. E. Johnson. 1995. Methane emissions from cattle. J. Anim. Sci. 73: 2483-2492. https://doi.org/10.2527/1995.7382483x
  14. Kim, D. R., J. J. Ha, J. T. Kim, and Y. H. Song. 2011. Evaluation on the greenhouse gas emission according to the intake levels of total mixed rations of hanwoo Cow. J. Anim. Sci. & Technol. (Kor). 53: 475-480. https://doi.org/10.5187/JAST.2011.53.5.475
  15. Kim, E. T., L. L. Guan, S. J. Lee, S. M. Lee, S. S. Lee, I. D. Lee, S. K. Lee, and S. S. Lee. 2015. Effects of flavonoid-rich plant extracts on in vitro ruminal methanogenesis, microbial populations and fermentation characteristics. Asian Australas. J. Anim. Sci. 28: 530-537. https://doi.org/10.5713/ajas.14.0692
  16. Kim, J. H., S. Y. Lee, and S. I. Cho. 2003. Anti-proliferative effect of Camellia japonica leaves on human leukemia cell line. Korea J. Herbol. 18: 93-93.
  17. Kim, O. R. 2004. Isolation and identification of antimicrobial compounds from Yucca smalliana Fern. Department of agricultural chemistry, Graduate School, Chonnam National University.
  18. Lee, H. Y., R. H. Kang, S. Y. Kim, S. I. Chung, and Y. S. Yoon. 2010. Platycodin D inhibits adipogenesis of 3T3-L1 cells by modulating kruppel-like factor 2 and peroxisome proliferator-activated receptor ${\gamma}$. Phytother. Res. 24: S161-S167. https://doi.org/10.1002/ptr.3054
  19. Lee, S. J. and J. N. Chung. 1977. Biochemical studies on ginseng saponins(IX). Korean Biochem. J. 10: 59-69. 2016.
  20. Lee, S. J., S. K. Lee, M. S. Kim, and S. S. Lee. Effects of nitrate-rich extracts on the in vitro ruminal fermentation and methane production. J. Agric. Life. Sci. 50: 95-105.
  21. Lee, S. Y., E. J. Hwang, G. H. Kim, Y. B. Choi, C. Y. Lim, and S. M. Kim. 2005. Antifungal and antioxidant activities of extracts from leaves and flowers of camellia japonica L. Korean J. Med Crop. Sci. 13: 93-100.
  22. Lee, S. J., D. S. Oh, D. H. Kim, J. U. Ok, S. K. Lee, J. H. Lim, and S. S. Lee. 2015. Effects of long-chain fatty acids on in vitro rumen microbial population, dry matter digestibility and methane production. J. Agric. Life. Sci. 49: 47-56.
  23. Lee, S. Y. and J. K. Ha. 2009. Mitigation strategies for enteric methane emission. Proceedings of 2009. Annual Congress of KSAST, KOREA. 1: 103-121.
  24. Lim, C. Y., S. Y. Lee, B. S. Pyo, and S. M. Kim. 2006. Fibrinolytic enzyme activity of extract from Camellia japonica L. Korean J. Med Crop. Sci. 14: 195-201.
  25. Lopez, S., F. M. McIntosh, R. J. Wallace, and C. J. Newbold. 1999. Effect of adding acetogenic bacteria on methane production by mixed rumen microorganisms. Anim. Feed Sci. Technol. 78: 1-10. https://doi.org/10.1016/S0377-8401(98)00273-9
  26. Makkar, H. P. S., M. Blummel, N. K. Borowy, and K. Becker. 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J. Sci. Food Agric. 61:161-165. https://doi.org/10.1002/jsfa.2740610205
  27. Wei, M., L. Ren, Z. Zhou, and Q. Meng. 2012. Effect of addtion of three plant extracts on gas production, ruminal fermentation, methane producion and ruminal digestibility based on in vitro technique. J. Anim. Vet. Adv. 11: 4304-4309.
  28. McCullough, M. E. and W. W. G.Smart Jr. 1968. Effects of intake of forge level on ruminal turnover rate, bacterial protein synthesis and duodenal amino acid flow in sheep. J. Anim. Sci. 62: 216.
  29. McDougall, E. I. 1948. The composition and output of sheep's saliva. Biochem. J. 43: 99-109. https://doi.org/10.1042/bj0430099
  30. Newbold, C. J., S. M. El Hassan, J. Wang, M. E. Ortega, and R. J. Wallace. 1997. Influence of foliage from African multipurpose trees on activity of rumen protozoa and bacteria. Br. J. Nutr. 78: 237-249. https://doi.org/10.1079/BJN19970143
  31. Odongo, N. E., R. Bagg, G. Vessie, P. Dick, M. M. Or-Rashid, S. E. Hook, J. T. Gray, E. Kebreab, J. France, and B. W. McBride. 2007. Long-term effects of feeding monensin on methane production in lactating dairy cows. J. Dairy Sci. 90: 1781-1788. https://doi.org/10.3168/jds.2006-708
  32. Ok, J. U. 2010. Analysis on various feed Additives utilization for methane reduction and improvement of fermentation characteristics in the rumen. Gyeongsang National University, Jinju, Korea.
  33. Ok, J. U., Y. C. Baek, K. H. Kim, S. C. Lee, Y. J. Seol, K. Y. Lee, C. W. Choi, C. O. Jeon, S. S. Lee, S. S. Lee, and Y. K. Oh. 2011. Effects of saponin contained plant extracts on ruminal fermentation characteristics and methane production. J. Anim. Sci. & Technol. (Kor). 53: 147-154. https://doi.org/10.5187/JAST.2011.53.2.147
  34. Pen, B., C. Sar, B. Mwenya, K. Kuwaki, R. Morikawa, and J. Takahashi. 2006. Effects of Yucca schidigera and Quillaja saponaria extracts on in vitro ruminal fermentation and methane emission. Anim. Feed Sci. Technol. 129: 175-186. https://doi.org/10.1016/j.anifeedsci.2006.01.002
  35. SAS. 1996. SAS User Guide. Release 6.12 edition. SAS Inst. Inc. Cary NC. USA.
  36. Sliwinski, B. J., M. Kreuzer, H. R. Wettstein, and A. Machmuller. 2002. Rumen fermentation and nitrogen balance of lambs fed diet containing plant extracts rich in tannins and saponins, and associated emissions of nitrogen and methane. Arch Anim Nutr. 56: 379-392.
  37. Sur, P., T. Chaudhuri, J. R. Vedasiromoni, A. Gomes, and D. K. Ganguly. 1998. Antiinflammatory and antioxidant property of saponins of tea Camellia sinensis (L.) O. Kuntze root extract. Phytother. Res. 12: 174-176.
  38. Theodorou, M. K., B. A. Williams, M. S. Dhanoa, A. B. McAllan, and J. France. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim. Feed Sci. Technol. 48: 185-197. https://doi.org/10.1016/0377-8401(94)90171-6
  39. Wina, E., S. Muetzel, and K. Becker. 2005. The impact of saponins or saponin-containing plant materials on ruminant production a review. J. Agric. Food Chem. 53: 8093-8105. https://doi.org/10.1021/jf048053d
  40. Zhao, H. L., K. Cho, Y. W. Ha, T. Jeong, W. S. Lee, and Y. S. Kim. 2006. Cholesterol-lowering effect of platycodin D in hypercholesterolemic ICR mice. Eur. J. Pharmacol. 537: 166-173. https://doi.org/10.1016/j.ejphar.2006.03.032