• 한국자원식물학회지
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• 제13권1호
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• pp.61-65
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• 2000

전라북도지방 소산 한약자원 식물은 91과 305속 470종 1아종 30변종 2품종으로 총 503종류가 본 조사결과 밝혀졌다. 각 과별로 그 숫자가 많은 종류는 국화과에 속하는 식물이 45종류로 가장 빈번하게 나타났고 그 다음은 장미과 식물이 30종류 그리고 콩과는 23종류의 순서였다. 전 조사지역에 고루 다수 분포하는 종류는 오미자, 마, 승마, 촛대승마, 족도리, 천남성, 둥굴레, 꿀풀, 박새를 들 수 있으며 집중적으로 나는 상태를 알아보기 위하여 정량조사를 실시한 결과, 예컨대, 더덕은 5m$\times$5m내에 30-50 개체가 덕유산 표고 약1300m 북서사면에서 조사되었다. 현재 심한 채취로 감소추세에 있는 식물은 둥굴레, 오미자, 더덕 등이고, 농가에서 재배중이거나 앞으로 개발 유망하며 가치가 높은 종류는 만삼, 가시오갈피, 천마, 복분자딸기, 더덕, 하수오 등이 있다. 요컨대 전라북도 지방에는 풍부하고 다양한 한약자원식물이 자연상태에 분포하고 있으며 이를 연구하고 집중적인 개발이 필요하다고 판단되었다.

• 대한본초학회지
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• 제25권4호
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• pp.85-93
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• 2010

Objectives : This study aimed at evaluation of the effects of Gastrodiae Rhizoma on brain edema and aquaporin water channel expressions in the brain. Methods : Brain edema following intracerebral hemorrhage (ICH) was induced by the stereotaxic intrastriatal injection of bacterial collagenase type VII in Sprague-Dawley rats. Then ethanol extract of Gastrodiae rhizoma was treated once a day for 3 days. Brain edema % and water contents, and cell size of neurons in the cerebral cortex were examined. Immuno-histochemistry was processed for AQP4, AQP1, and AQP9 expressions in the brain sections and area % of immuno-labeling was analyzed with image analysis. Results : 1. Ethanol extract of Gastrodiae Rhizoma reduced brain edema of ICH induced rats significantly. 2. Ethanol extract of Gastrodiae Rhizoma reduced excessive brain tissue water contents of ICH induced rats significantly. 3. Ethanol extract of Gastrodiae Rhizoma reduced cellular edema of neurons in cerebral cortex of ICH induced rats significantly. 4. Ethanol extract of Gastrodiae Rhizoma reduced AQP4 immuno-positive area % in cerebral cortex and external capsule of ICH induced rat brain significantly. 5. Ethanol extract of Gastrodiae Rhizoma reduced AQP9 immuno-positive area % in glia limitans externa of ICH induced rat brain significantly. Conclusions : These results suggest that Gastrodiae Rhizoma reveals protective effects against brain edema and cytotoxic edema of neurons by means of down-regulation of AQP4 expression in the brain.

• 대한한방내과학회지
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• 제26권3호
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• pp.699-707
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• 2005

Vertebrobasilar insuffciency(VBI) is also refered to as vertebrobasilar transient ischemic attack(V-B TIA). Vertebrobasilar insufficiency is often undiagnosed or misdiagnosed due to complexity of the symptoms. Transcranial doppler(TCD) can be used to possible to record the blood flow velocities in the cerebral arteries through the intact cranium. Transcranial doppler and magnetic resonance angiography(MRA) are both noninvasive techniques that can be used in vertebrobasilar insufficiency, but transcranial doppler is more sensitive with respect to evaluating stenotic lesion of the arteries than magnetic resonance angiographv. Diagnostic values of transcranial doppler were assessed in patients with vertebrobasilar insufficiency. A case of vertebrobasilar insufficiency is here reported. A 62-year-old man with dizzness, nausea and tremor because of vertebrobasilar insufficiency was admitted at Wonkwang Oriental Medicine Hospital. He was treated with the herbal medicine Chunmagudung-um and with acupunture. Results showed improvement of symptoms and favorable transcranial doppler readings. The process used in this successful treatment is here described.

• 생약학회지
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• 제48권3호
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• pp.232-236
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• 2017

Gastrodia elata has been widely used as a traditional medicinal herb. However, in Korean Pharmacopeia, neither a marker constituent nor an analytical procedure for G. elata has been established. In this study, we suggest gastrodin and gastrodigenin as marker constituents of G. elata, and propose an analytical procedure for simultaneous quantification of these constituents by high-performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV). The analytical method was validated for its linearity, precision, accuracy, and specificity. Based on the validated method, gastrodin and gastrodigenin in 14 commercial G. elata samples were quantified.

• 동의신경정신과학회지
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• 제25권3호
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• pp.243-252
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• 2014

Objectives: This study was designed to investigate the dose-dependent effects of Gastrodia elata Blume for memory improvement. Methods: This study was a 12-weeks, double blind, and comparative clinical study. Those who were eligible worked with a group of healthy seniors, all 60 years of age or older. 22 subjects were randomized either to Gastrodia elata Blume powder form that was steeped in hot water or placebo. We measured the faculty of memory by using MMSE-K, Digit Span, Letter Fluency Test, Word List Memory Test, and Trail Making Test, and again after 12 weeks. Results: 1) Neither Gastrodia elata Blume groups nor control have a difference in MMSE-K, Digit Span, Letter Fluency Test, and Trail Making Test. 2) Gastrodia elata Blume group showed significant advances in immediate recall 1 and 2 of Word List Memory Test, and 3 g group show better results than the 4 g group. 3) 4 g Gastrodia elata Blume group showed significant advances in the recognition of Word List Memory Test. Conclusions: The results suggest that positive effects on memory improvement due to Gastrodia elata Blume depend on the amount.

• 대한암한의학회지
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• 제14권1호
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• pp.45-52
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• 2009

Lung cancer is the most common primary tumor of brain metastasis. Metastasis to the brain is a frequent complication of non-small cell lung cancer (NSCLC) occuring in 12 ~ 18% of patient. The incidence of brain metastasis is rising because of longer survival of cancer patients as a result of the increase in early diagnosis of primary tumors and aggressive management, and improvements in imaging quality and accessibility such as widespread use of MRI. The most common symptoms are gradual onset of headache, focal weakness, and mental changes. Surgery, chemotherapy, radiotherapy and steroid therapy are treatment of NSCLC with brain metastasis. We report a patient with NSCLC metastasis to the brain. This patient underwent chemotherapy, radiotherapy and steroidtherapy. However, the patient requested oriental medical treatment for the patient's continuous headache and disease. We administered Banhabaekchulchoenma-tang gagambang with Allergen-removed Rhus Verniciflua Stokes(aRVS). The patient showed remarkable improvement in terms of frequency and severity of headache. Further study will be needed in order to determine the long-term effectiveness of oriental medical treatment on cancer patient with headache.

• 한국약용작물학회지
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• 제28권1호
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• pp.29-37
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• 2020

Background: Although Gastrodia elata contains the valuable, bio-active compounds gastrodin and 4-hydroxybenzyl alcohol (4-HBA), it also contain ρ-cresol, which is a major source of an objectionable pig slurry-like odor. Accordingly, this study was performed to determine the deodorization effect of Diaion HP-20 resin on a G. elata extract. Methods and Results: Using Diaion HP-20 with an increasing concentration of ethanol, an extract of G. elata was fractionated into 15 fractions, which were anlayzed to determine their concentrations of active compounds and ρ-cresol. The gastrodin, 4-HBA, total phenolics, and ρ-cresol concentrations of the G. elata extract were 403.13, 408.15, 2,476.09, and 11.77 ㎍/㎖, respectively. Among the different fractions, gastrodin and 4-HBA were detected in fractions 3 to- 6, and 4 to- 10, respectively whereas total phenolics were detected in all samples, with fractions 5 to- 7 being characterized by higher concentrations than were the other fractions. ρ-Cresol was detected in fractions 12 to- 15, at concentrations ranging from 0.39 ㎍/㎖ to 6.05 ㎍/㎖ with the highest concentration detected in fraction 14. The fractions containing ρ-cresol could be selectively separated from the G. elata extract without the loss of major bio-active compounds such as gastrodin and 4-HBA. Conclusions: The results of this study indicate that a deodorizing method using Diaion HP-20 resin can be considered for improving the sensory qualities of G. elata extract without a loss of the major bio-active compounds.

• Journal of Acupuncture Research
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• 제25권1호
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• pp.1-14
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• 2008

Objectives : Delayed neuronal death(DND) of pyramidal neuronsin the CA1 regions of the hippocampus has been extensively studied following global brain ischemia, whereas little is known about DND in this highly vulnerable brain region after focal brain ischemia. The aim of the present study was to investigate the effect of Gastrodiae Elata(GA) pharmacopuncture on hippocampal neuronal apoptosis in rats with focal brain ischemic injury. Materials and methods : The neuroprotective effects of water extracts of GA were investigated in middle cerebral artery occlusion(MCAo) of Sprague-Dawley(SD) rats. Seventy-five healthy SD ratswere randomly divided into five groups following MCAo : control group with focal ischemia, saline injection group, pharmacopuncture group GA-1($0.0007mg/m{\ell}/g$), pharmacopuncture group GA-2($0.00035mg/m{\ell}/g$), pharmacopuncture group GA-3($0.00014mg/m{\ell}/g$). Results : The intensity of mGluR5 increased in the GA-1 group. The intensity of Bax and the Bax/Bcl-2 ratio decreased in the GA-1 group. The intensity of Bcl-2 increased in all the GA groups. The density of neurons stained by Cresyl violet and ChAT increased in the GA-1 group. Conclusions : Our study suggests that GA pharmacopuncture at $GB_{20}$ showed anti-apoptotic and neuroprotective effects on cholinergic neuronsin focal cerebral ischemia caused by stroke in SD rats.

• 한국산업보건학회지
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• 제27권2호
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• pp.115-122
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• 2017

Objectives: This study was designed to evaluate the changes in the particle filtration efficiencies of five cloth masks (4 plate types, 1 cup type) with an increasing number of machine washings and the degree of cloth expansion. Methods: NaCl aerosols were generated using an atomizer and passed through cloth masks in a dynamic aerosol chamber. Particle concentrations were measured both before and after for the cloth masks using an optical particle counter (OPC) in the size range of $0.3{\sim}10{\mu}m$. Results: In the original condition, the filtration efficiencies of the five cloth masks were A: 20.1%, B:30.9%, C: 25.0%, D: 26.5%, and E: 40.9%. As the number of washings increased in the order of one, two, and four times, the filtration efficiencies of cloth mask C increased. The filtration efficiency of A, D, and E increased after the first washing. With the exception of B, the filtration efficiency of cloth masks increased after the second washing and those of all cloth masks increased after the fourth washing. This might be caused by the fibers untangling from the yarn and being freed at one end. In this status, the packing density of the textile will not change, but the distances between fibers will increase, which might bring about an increase in filtration efficiency. When the cloth masks were extended by 10% and 20% in one direction, the filtration efficiencies of cloth masks B, D, and E decreased at 10% extension, and those of all cloth masks decreased at 20% extension. When the cloth masks were expanded by 10% and 20% in two directions, the filtration efficiencies of all cloth masks decreased by at least 34.7% at 10% extension, and by at least 60.9% at 20% extension Conclusions: The filtration efficiency of cloth masks could decrease after one to two machine washings, but will increase after four washings in comparison with their original performances. The filtration efficiency of cloth masks will decrease when they are expanded, such as when stretching over the nose during wearing status.

• 동의생리병리학회지
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• 제23권5호
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• pp.1080-1086
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• 2009

This study was designed to investigate the effects of Gastrodiae Elata Pharmacopuncure at GB20 on motor control and cognitive dysfunction recovery after mild traumatic brain injury in rats. Rats were divided into three groups; (1) no treatment after traumatic brain injury(experiment I), (2) Treatment with NPA after traumatic brain injury(experiment II), (3) Treatment with GEP after traumatic brain injury(experiment III). In our study, we carried out behavioral test(Rotarod, Morris water maze) and immunohistochemistry study of the change BDNF in the hippocampus(pre, $7^{th}$, $14^{th}$ day). In Rotarod test(motor control function) was significantly increased in the experimental group III as compared with experimental group I, II on $7^{th}$(p<0.01) and $14^{th}$ day(p<0.001). In Morris water maze test(cognitive function) was significantly decreased in the experimental group III as compared with experimental group I, II on $14^{th}$ day(p<0.001). In immunohistochemistric response of BDNF in the hippocampus, the experimental group III was more immune response than the other groups on $14^{th}$ day. These results imply that Gastrodiae Elata Pharmacopuncure at GB20 can play a role in facilitating recovery of motor control and cognitive function after mild traumatic brain injury in rats.