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Simultaneous Determination of Gastrodin and Gastrodigenin in Gastrodia elata by HPLC-UV  

Kang, Kyo Bin (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Jun, Jin Bum (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Yoo, Guijae (College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University)
Kwon, Euna (Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University Hospital)
Yun, Jun-Won (Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University Hospital)
Kang, Byeong-Cheol (Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University Hospital)
Kim, Seung Hyun (College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University)
Sung, Sang Hyun (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Jang, Young Pyo (College of Pharmacy, Kyung Hee University)
Yang, Heejung (College of Pharmacy, Kangwon National University)
Publication Information
Korean Journal of Pharmacognosy / v.48, no.3, 2017 , pp. 232-236 More about this Journal
Abstract
Gastrodia elata has been widely used as a traditional medicinal herb. However, in Korean Pharmacopeia, neither a marker constituent nor an analytical procedure for G. elata has been established. In this study, we suggest gastrodin and gastrodigenin as marker constituents of G. elata, and propose an analytical procedure for simultaneous quantification of these constituents by high-performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV). The analytical method was validated for its linearity, precision, accuracy, and specificity. Based on the validated method, gastrodin and gastrodigenin in 14 commercial G. elata samples were quantified.
Keywords
HPLC analysis; Gastrodia elata; Gastrodin; Gastrodigenin; Method validation;
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