• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.303-311
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• 2017

In this study, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analyses were used for evaluation of genetic diversity of 61 kiwifruit (Actinidia spp.) germplasms including domestic and overseas collection cultivars. Forty RAPD primers were detected in a total of 230 polymorphic bands with an average of 5.75. Thirty-two SRAP primer combinations were detected in a total of 204 polymorphic bands with an average 6.38. By unweighted pair-group method arithmetic average cluster analysis using 434 polymorphic bands, kiwifruit germplasms were classified in three groups with similarity value of 0.680. Cluster I consisted of 46 kiwifruit germplasms belonging to A. deliciosa, A. chinensis, A. deliciosa ${\times}$ A. arguta, A. chinensis ${\times}$ A. arguta, and A. chinensis ${\times}$ A. deliciosa. Cluster II consisted of seven germplasms belonging to A. arguta and 'Skinny Green', a cultivar derived from a cross between A. arguta and A. deliciosa. Cluster III consisted of seven germplasms belonging to A. rufa, A. hemsleyana, A. macrosperma, A. polygama, and A. eriantha. Genetic similarity values among tested kiwifruit germplasms ranged from 0.479-0.991, and average similarity value was 0.717. Similarity value was highest (0.991) between NHK0038 (A. deliciosa) and NHK0040 (A. deliciosa), and lowest (0.479) between 'Hayward' (A. deliciosa) and K5-1-22 (A. arguta).

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.107-115
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• 2004

To develop universal primers for phylogenetic analysis and species-specific marker for breeding program of kiwifruit, eighteens primers were designed from kiwifruit genome-specific repeat sequences. Seven species including twenty two varieties collected from native eastern Asia were examined using 18 to 22 mer kiwifruit target(KT) primers. among eighteen primers, we selected seven primers for phylogenetic relationship. The genus Actinidia was divided into two large groups; group I,A. arguta, A. melanandra, A. kolomikta, and A. marcrosperma, characterized by the non-hair in fruits and loaves or a few pubescences only in young stage, which belongs to the section Leiocarpae, and group II, A. chinensis, A. deliciosa, and A. eriantha, characterized by a lot of hairs only in young fruit stage and with a lot of hairs or fuzzes in leaves and branches, which belongs to the section Stellatae. Group II especially belongs to the series Perfectae of the section Stellatae and was divided into two subgroups; subgroup I containing A. chinensis and A. deliciosa, and subgroup II containing A. eriantha. In contrast, the two species, A. chinensis and A. deliciosa, which are known to have common parents, were divided into two independent subgroups with 80％ of a similarity value. On the other hand, we selected KT6F for variety specific bands, KT12E primers for 'Hayward' and 'Tomuri'. KT7F or KT12F primers were useful for analysis of inheritance pattern in kiwifruit cross-breeding. We suggest that these primers will be a powerful tool for elucidating phylogenetic relationship and selection of novelty kiwifruit in a breeding program.

• Journal of the East Asian Society of Dietary Life
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• v.18 no.1
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• pp.49-57
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• 2008

In order to control or kill insects and pests, control ripening, and delay spoilage, kiwifruits were irradiated with gamma rays at 0.1, 0.3, and 0.5 kGy. Vitamin C, hydrogen donating activity, sugar content, organic acid content, texture, chromaticity, and sensory qualities were examined in the fruits over 2 weeks of storage at 20$^{\circ}C$. Vitamin C content and hydrogen donating activity decreased during the storage period, but they were not reduced by the irradiation process. Total sugar and reducing sugar contents increased as the storage period increased; however, these were also unaffected by irradiation. Organic acid content significantly decreased as a result of irradiation, and all samples had decreases in organic acids over the storage period. Hardness decreased with the storage period as well as with increasing doses of irradiation. In terms of Hunter's color values, irradiation increased the $L^*-\;and\;a^*-$ values in the initial storage period, but it did not have any significant effect on these values during the 2 weeks of storage. When compared with control samples, the irradiated samples had lower color and overall acceptability scores just after irradiation (week 0). However, during the storage period, the irradiated samples had higher scores for smell, taste, texture, and overall acceptability than the control. In conclusion, based on the results, gamma irradiation up to 0.5 kGy is the recommended treatment to maintain the overall quality attributes kiwifruit.

• Applied Biological Chemistry
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• v.39 no.6
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• pp.466-471
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• 1996

The survey on the chitinolytic activity of some plants was performed for the purpose of obtaining some reliable and inexpensive sources of chitinase. Rice, soybean for sprouting, kiwi fruit, almond and crude papain were investigated. Rice bran, seed coat of the soybean and the pericarp of kiwi fruit showed a considerable activity, while the bean after the removal of the seed coat, the mixture of rice integument and endosperm, polished rice, and defatted soybean powder didn't have any detectable activity. These crude enzymes have shown to contain both endo- and exochitinase activity. The effects of pH and temperature on the enzyme activity were variable. Furthermore we have observed the chitosanolytic activity from these enzyme Preparations. The rice bran had the highest activity in the enzymatic degradation of chitosan, and seed coat of soybean and the pericarp of kiwi fruit followed. On the basis of the fact that crude papain was not only commercially available but also the most potent in the endochitinase activity and the lowest in the exochitinase activity, we could conclude that crude papain was considered as the most suitable source of the chitinase among plants studied in this paper. In addition, rice bran was worth further investigation from the point of utilizing agricultural by-product.

• Korean journal of applied entomology
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• v.33 no.3
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• pp.148-152
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• 1994

This study was carried out to investigate insect specles of Kiwi fruit, and ecological characteristicsof Stathmopoda m Chonnam province from 1991 to 1993 Twelve families and thirteen specieswere identified during the study. Sfothmopoda aurijerello and mulberp scale were key insect pestsamong them. The body sizes of each stage of S. a~irijereilo was measured and found to be 0.12 mmfor egg, 9.8 mm for mature lawa. 5.9 mm for pupae and 123mm for adult with opened wings.S, ouriferelia appers to have two generations a yea Adults occut~ed from late May to midJulyand mid-August to early September Mth two peaks m early to midJune and late August. Changein age structure (% lawe: % pupae) over time was 100: 0 in earlyJul, 96. 1 . 3 9 in midduly. 64.9:35 1 in late July. 19.8 . 80 2 in early August, and 0 : 100 in mld-August Damaged fruit was 4 6%in early July, increase above 40% 1" midJuly, and then the damage surpassed the damage threshold.The rate of fruit damaged was 45.9%, and there wbi no significant dtfferences among countiesDamaged parts of fruits were manly fruit apex Mth 70% and followed by fruit stalk w~th 11.1%.The rate of fruit damaged by varieties were Hayward with 25.7%. Monty with 17 5%. Hyangrokwith 13.9%, Abott with 9.3% and Bruno with 6.0%. with 9.3% and Bruno with 6.0%.

• Food Science and Preservation
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• v.14 no.3
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• pp.294-300
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• 2007

Three varieties of soy sauce were prepared using saps of bamboo, xylem and gorosoe, with sea salt and bamboo salt, in May. Soy sauce was fermented in the traditional manner. Homemade soy sauce was analyzed after 3 months of aging. The pH and amino-type nitrogen content of soy sauce fermented with xylem sap and sea salt were very high, and malic acid was dominant among four types of organic acid. The content of calcium in soy sauce fermented with bamboo sap and sea salt was fourfold greater than that of the control. The contents of total amino acids in the soy sauces fermented with xylem sap and gorosoe, with the addition of sea salt instead of water, were 2-fold and 1.4-fold greater than that of ordinary soy sauce, respectively. In the DPPH assay, the antioxidant effect of soy sauce fermented with bamboo sap and sea salt was higher than that of other samples. Sensory evaluation showed that the overall acceptability of soy sauce fermented with gorosoe and sea salt was best, among the samples tested.

• Journal of Bio-Environment Control
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• v.10 no.1
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• pp.1-9
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• 2001

Fruit growth in kiwifruit shows double sigmoid curve, but it does not certainly indicate as years. Therefore, I though the reason to be easy to the effect of water state change in kiwifruit, investigated diurnal change in water status of fruit tissues with an isoipiestic psychrometers against the fruit growth stage of kiwifruit in 1995 and 1996. Diurnal change in the fruit tissue water potential were little, but violent for fruti growth state III in 1996. The potential of two years dropped gradually approach to harvest time. On the other hand, osmotic potential of the tissues indicated to very similar to water potential, dropped rapidly -1.5MPa before dawn, recovered -1 MPa after 3 h on October 14, were -1~-1.7 MPa at the fruit commercial harvest in 1995. It had a tendency to lower in 1996 than in 1995. It was recorded to the minimum air temperature at the first for an autumn in 1995; 13$^{\circ}C$ from the middle night of October 13 to dawn of October 14. Leaves water potential, which is related to water status of xylem, nearly fell below -1 MPa at before dawn from stage II in 1996. However, it fell so low only at commercial in 1995. At the stage II, osmotic potential and ascent of the turgor pressure was high than 1995-fruit. There parameter suggested that three of kiwifruit in 1996 were status of water stress for stage III. The results from this study indicated that difference of fruit growth between 1995-fruit and 1996-fruit was affected by water status of the fruit tissues, which was influenced by weather condition.

• Korean Journal of Plant Resources
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• v.23 no.2
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• pp.165-171
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• 2010

Oxidative stress induced by reactive oxygen intermediates has been implicated in a variety of human diseases including neurodegenerative disorders, cancer, cardiovascular and respiratory diseases, and mode of action of environmental toxicants. Tert-butylhydroperoxide (t-BHP) is an organic lipid hydroperoxide analogue, which is commonly used as a pro-oxidant for evaluating mechanisms involving oxidative stress in cells and tissues. In this study, the underlying mechanisms involved in the protective effects of Hwabuk 94 kiwifruit (Actinidia deliciosa cv. 'Hwabuk 94'), which is cultivated in Jeju, on the t-BHP-induced cytotoxicity in PC12 cell. The pretreatment of rat pheochromocytoma cell line PC12 with Hwabuk 94 extract ($1-100\;{\mu}g/ml$) resulted in a significant recovery from t-BHP-induced cell death and increased Bcl-2 and procaspase-3 expression, whereas the expression of Bax and cleaved PARP were decreased in a dose-dependent manner compared to the control. Furthermore, Hwabuk 94 inhibited the t-BHP-induced p38 MAP kinase and extracellular signal-regulated kinase 1/2, but not c-Jun N-terminal kinase activations. Finally, these findings suggest that Hwabuk 94 kiwifruit might attenuate t-BHP-induced PC12 cell cytotoxicity, at least in part, through the inhibition of signaling pathways mediated by the ERK1/2 and p38 MAP kinase.

• Research in Plant Disease
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• v.10 no.4
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• pp.310-312
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• 2004

Pseudomonas syringae pv. actinidiae is the causative agent of bacterial canker in kiwifruit. A nested PCR detection method that uses primers designed from the cfl gene, involved in production of the phytotoxin coronatine, was applied on soil samples. These primers yielded 665 and 310-bp fragments in consecutive PCR amplification step with DNA from soil inoculated with Korean strain of P. syringae pv. actinidiae. This system was applied to survey soil samples from a kiwifruit orchard destroyed by bacterial canker. A specific 310-bp PCR product was obtained from all six samples of soil tested.

• Korean Journal of Organic Agriculture
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• v.24 no.2
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• pp.253-262
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• 2016

The SS (Bacillus subtilis 10%), WS (Microbial extract 70%), and DS (Sulfur 78%) agents were selected by mycelial growth inhibitory effect test against kiwifruit soft rot pathogen (B. dothidea) with 11 kinds of environment-friendly agricultural materials on PDA medium for 10 days. They showed at 94.2%, 65.2%, 58.9%, respectively. The control value of WS and SS agents were better than DS in storage experiment. It was effective SS and WS single application, DS-WS and WS-SS alternate application in the field trial.