Browse > Article
http://dx.doi.org/10.5423/RPD.2004.10.4.310

Detection of Pseudomonas syringae pv. actinidiae in Soil on the Basis of PCR Amplification  

Han, Hyo-Shim (Department of Biology Sunehon National University)
Koh, Young-Jin (Department of Applied Biology, Sunehon National University)
Jung, Jae-Sung (Department of Biology Sunehon National University)
Publication Information
Research in Plant Disease / v.10, no.4, 2004 , pp. 310-312 More about this Journal
Abstract
Pseudomonas syringae pv. actinidiae is the causative agent of bacterial canker in kiwifruit. A nested PCR detection method that uses primers designed from the cfl gene, involved in production of the phytotoxin coronatine, was applied on soil samples. These primers yielded 665 and 310-bp fragments in consecutive PCR amplification step with DNA from soil inoculated with Korean strain of P. syringae pv. actinidiae. This system was applied to survey soil samples from a kiwifruit orchard destroyed by bacterial canker. A specific 310-bp PCR product was obtained from all six samples of soil tested.
Keywords
cfl gene; nested PCR; Pseudomonas syringae pv. actinidiae; Soil;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Serizawa, S., Ichikawa, T., Takikawa, Y., Tsuyumu, S. and Goto, M. 1989. Occurrence of bacterial canker of kiwifruit in Japan: Description of symptoms, isolation of the pathogen and screening of bactericides. Ann. Phytopath. Soc. Japan 55: 427-436   DOI
2 Takikawa, Y., Serizawa, S., Ichikawa, T., Tsuyumu, S. and Goto, M. 1989. Pseudomonas syringae pv. actinidiae pv. nov.: The causal bacterium of canker of kiwifruit in Japan. Ann. Phytopath. Soc. Japan 55: 437-444   DOI
3 Tamura, K., Takikawa, Y., Tsuyumu, S. and Goto, M. 1989. Characterization of the toxin produced by Pseudomonas syringae pv. actinidiae, the causal bacterium of kiwifruit canker. Ann. Phytopath. Soc. Japan 55: 512
4 Han, H. S., Koh, Y. J., Hur, J. S. and lung, J. S. 2003. Identification and characterization of coronatine-producing Pseudomonas syringae pv. actinidiae. J. Microbiol. Biotechnol. 13: 110-118
5 고영진, 차병진, 정희정, 이동현. 1994. 참다래 궤양병의 격발 및 확산. 한식병지 10: 68-72
6 Bender, C, Liyanage, H., Palmer, D., Ullrich, M., Young, S. and Mitchell, R. 1993. Characterization of the genes controlling biosynthesis of the polyketide phytotoxin coronatine including conjugation between coronafacic and cofonarnic acid. Gene 133: 31-38   DOI   ScienceOn
7 고영진, 박숙영, 이동현. 1996. 우리나라 참다래 궤양병 발생 특성 및 수간 주입에 의한 방제. 한식병지 12: 324-330
8 정재성, 한효심, 조윤섭, 고영진. 2003. Nested PCR을 통한 참다래 궤양병균(Pseudomonas syringae pv. actinidiae)의 검출. 식물병연구 9: 116-120
9 Opgenorth, D. C, Lai, M., Sorrell, M. and White, J. B. 1983. Pseudomonas canker of kiwifruit. Plant Dis. 67: 1283-1284   DOI
10 Sawada, H., Takeuchi, T. and Matsuda, I. 1997. Comparative analysis of Pseudomonas syringae pv. actinidiae and pv. phaseolicola based on phaseolotoxin resistant ornithine carbamoyltransferase gene (argK) and 16S-23S rRNA intergenic spacer sequences. Appl. Environ. Microbiol. 63: 282-288
11 Mazarei, M. and Mostofipour, P. 1994. First report of bacterial canker of kiwifruit in Iran. Plant Pathology 43: 1055-1056   DOI   ScienceOn
12 고영진. 1995. 참다래의 주요 병. 식물병과 농업 1: 3-13
13 Prosen, D., Hatziloukas, E., Schaad, N. and Panopoulos, N. J. 1993. Specific detection of Pseudomonas syringae pv. actinidiae DNA from bean seed by polymerase chain reaction based amplification of a phaseolotoxin gene region. Phytopathology 83: 965-970   DOI   ScienceOn
14 Scortichini, M. 1994. Occurrence of Pseudomonas syringae pv. actinidiae on kiwifruit in Italy. Plant Pathology 43: 1035-1038   DOI   ScienceOn