• Title/Summary/Keyword: 집락추출

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Antibacterial and phagocytosis control of natural extracts on S. mutans (S. mutans에 대한 천연추출물의 항균 및 탐식작용조절)

  • Kim, Min-Young;Hwang, Hye-Jeong;Kang, Kyung-hee
    • Journal of the Korea Convergence Society
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    • v.13 no.5
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    • pp.113-117
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    • 2022
  • In this study, the antibacterial and phagocytosis regulation effects of Hordeum vulgare extract and pine needle extract on S. mutans, the causative bacteria of dental caries, were investigated. Ethanol extracts of domestic Hordeum vulgare powder and pine needle powder were used, and the antibacterial and phagocytic ability against S. mutans was confirmed according to the concentration of the extracts. As a result, S. mutans colony formation did not show a significant difference in the Hordeum vulgare extract but was significantly decreased in the pine needle extract. As a result of confirming the phagocytic ability of THP-1 cells for S. mutans, there was no significant difference in the Hordeum vulgare extract, but the phagocytic ability of immune cells was improved in the pine needle extract. Therefore, it suggests that pine needle extract can be used as a material for preventing dental caries.

Anti-bacteria effect of green tea, mulberry, and mate leaves extracts on S. mutans (녹차잎, 뽕잎, 마테잎 추출물이 S. mutans에 미치는 항균효과)

  • Kim, Sung-Sook;Won, Ji-Hye;Lee, Go-Eun;Lee, Ru-Ri;Lee, Sun-Mi;Lee, Jin-Hee;Kang, Kyung-hee
    • Journal of Digital Convergence
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    • v.15 no.1
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    • pp.347-353
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    • 2017
  • This study aims to figure out how green tea leaves, mulberry leaves, and mate leaves that are often taken around in drinking tea influence on S. mutans known as dental caries causative bacteria. After vaccinating 1% of S. mutans on the extract added badge and incubating 6 hours and 10 hours in $37^{\circ}C$, it calculated absorbance and bacterial colony number. Extract of green tea leaves and mate leaves were added with 0, 0.5, 1.0, 2.0, and 4.0% of concentration. As a result of growth suppressive effect of S. mutans, it may be observed that colony number significantly decreases in higher concentration. When it calculated bacterial colony number with adding 2% extract after 10 hours, 99.0% for green tea leaves, 97.1% for mulberry leaves, and 89.6% for mate leaves appeared.

Measuring stratification effects for multistage sampling (다단추출 표본설계의 층효율성 연구)

  • Taehoon Kim;KeeJae Lee;Inho Park
    • The Korean Journal of Applied Statistics
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    • v.36 no.4
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    • pp.337-347
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    • 2023
  • Sampling designs often use stratified sampling, where elements or clusters of the study population are divided into strata and an independent sample is chosen from each stratum. The stratification strategy consists of stratification and sample allocation, which are important issues that are repeatedly considered in survey sampling. Although a stratified multistage sample design is often used in practice, the literature tends to discuss simple sampling in terms of stratum effects or stratum efficiency. This study examines an existing stratum efficiency measure for two-stage sampling and further proposes additional stratum efficiency measures using the design effect model. The proposed measures are used to evaluate the stratification strategy of the sample design for high school students of the 4th Korean National Environmental Health Survey (KoNEHS).

Biocidal Effects of Chlorine Dioxide on Isolated and Identified Pathogens from Nosocomial Environment - Biochemical and Technical Covergence (병원내 환경으로부터 분리 및 확인된 병원균에 대한 이산화염소의 살균 효과 - 생화학 및 기술 융합)

  • Song, Kyoung-Ju;Jung, Suk-Yul
    • Journal of Digital Convergence
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    • v.15 no.6
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    • pp.339-344
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    • 2017
  • In this study, microorganisms were isolated from nosocomial environment and are identified by biochemical analysis as the part of biochemical and technical convergence. Microorganisms were collected at intense care unit of general hospital located in Pyeongtak (2014.11.28. - 2014. 11. 30). Using a VITEK2 equipment of biochemical approaches, eleven microorganisms e.g., Micrococcus luteus (or M. lylae), Granulicatella adiacens (M. luteus or M. lylae), Staphylococcus caprae, Sphingomonas paucimobilis, Kocuria kristinae, G elegans, Aerococcus viridans (or Staphylococcus arlettae), Methylobacterium spp., Dermacoccus nishinomiyaensis (or Kytococcus sedentarius), Kocuria kristinae (or M. luteus, M. lylae), Pseudomonas oryzihabitans were identified. And then identified bacteria plates were applied with a plastic stick, so called with "FarmeTok (medistick/Puristic) to produce ClO2. ClO2-releasing plastic stick showed the very strong inhibition of bacterial growth with about 99.9%. There were no bacterial colonies on the ClO2-incubated plate. Taken together, it is suggested that chlorine dioxide should be very strong inhibitor to microorganisms of nosocomial infections.

Genotoxicological Safety of the ${\gamma}$-ray Irradiated Astragali Radix, Glycyrrhizae Radix and Aurantii nobilis Pericarpium in the Ames Test (Ames test를 이용한 감마선 조사 황기, 감초 및 진피의 유전독성학적 안전성 평가)

  • 함연호;육홍선;조성기
    • Food Science and Preservation
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    • v.8 no.1
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    • pp.54-59
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    • 2001
  • The three medicinal herbs, Astragali Radix, Glycyrrhizae Radix and Aurantii nobilis Pericarpium, irradiated with γ-rays have been tested for their possible genotoxicity. The hot water extracts of the 10 kGy γ-ray irradiated herbs were examined in the Salmonella mutagenicity test(histidine reversion assay; Ames test) using Salmonella. typhimurium TA98 and TA100 as tester strains. No mutagenicity was detected in this assay both with and without metabolic activation. The safety of the herbs irradiated with γ-rays at tactical doses needs to be evaluated in further tests of genotoxicity in vivo and chronic and reproductive toxicity.

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Relationship of Rhythmic Exercise Participation, Physical Image and Self-Esteem among Women (여성의 리듬운동 참가와 신체이미지 및 자아존중감의 관계)

  • Song, Kang-young;Koo, Kyoung-ja;Kim, Kyong-sik
    • Proceedings of the Korea Contents Association Conference
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    • 2010.05a
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    • pp.158-160
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    • 2010
  • 이 연구의 목적은 여성의 리듬운동 참가와 신체이미지 및 자아존중감의 관계를 규명하는데 있다. 본 연구는 2009년 서울 경기도 지역 2009년 서울 경기도지역에 소재하고 있는 스포츠센터, 일반동호회, 지역동호회, 댄스학원, 일반인을 대상으로 리듬운동에 참여하고 있는 20~50대 성인여성을 모집단으로 설정한 다음, 집락무선표집법을 이용하여 300명을 추출 하였다. 결론은 다음과 같다. 첫째, 리듬운동 참가여성은 비참가여성에 비해 신체이미지 및 자아존중감을 더 긍정적으로 평가한다. 둘째, 리듬운동 참가기간은 체형, 만족, 유연성요인에 영향을 미치고 있으며, 참가강도는 체형과 근력요인에 영향을 미친다. 셋째, 리듬운동참가기간은 자아존중감에 영향을 미치며, 참가빈도는 부적 영향을 미치는 것으로 나타났다. 넷째, 여성의 리듬운동 참가정도는 신체이미지 그리고 자아존중감에 인과적 영향을 미친다.

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The Effects of Organizational Culture of Aviation Security Workers on Organizational Effectiveness (항공보안업무 종사자의 조직문화가 조직유효성에 미치는 영향)

  • Cho, Yong-Hoon;Lee, Chi-Young;Park, Su-Hyeon
    • Proceedings of the Korean Society of Disaster Information Conference
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    • 2023.11a
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    • pp.49-50
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    • 2023
  • 본 연구는 항공보안업무 종사자의 조직문화가 조직유효성에 미치는 영향을 규명하기 위하여 항공보안검색요원 300명을 대상으로 집락표본추출법(cluster sampling method)을 통해 자료를 수집하여 분석하였다. 수집된 설문지 중 표기오류, 누락, 전체 항목의 동일 표기 등을 분류하여 최종적으로 272부를 분석에 사용하였다. 연구결과 조직문화는 조직유효성에 정(+)적인 영향을 미치는 것으로 나타났다. 조직문화의 하위요인인 합리문화, 합의문화, 발전문화는 조직유효성의 하위요인인 직무만족에 영향을 미치는 것으로 나타났으며, 위계문화는 영향을 미치지 않는 것으로 나타났다. 또한 합의문화, 위계문화, 발전문화 순으로 조직몰입에 영향을 미쳤으나 합리문화는 통계적으로 유의한 영향을 미치지 않는 것으로 나타났다.

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Preventive effects of shiitake mushroom extract on candida stomatitis (칸디다성 구내염에 대한 표고버섯 추출물의 예방효과)

  • Yoo, Hyun-Jun
    • Journal of Dental Rehabilitation and Applied Science
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    • v.37 no.3
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    • pp.123-129
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    • 2021
  • Purpose: The purpose of this study was to investigate antifungal activity of shiitake mushroom yeast and hyphal type of Candida albicans. Materials and Methods: The extract from shiitake mushroom was collected by drying the supernatant after soaking shiitake mushrooms in water or ethanol. The antifungal activity of the extracts against yeast type of C. albicans was investigated by the susceptibility assay using microplate. C. albicans biofilm was formed on 12-well plate using Ham's F-12 medium in CO2 incubator and treated with the ethanol extract. Furthermore, C. albicans biofilm was formed on denture base resin disk and treated with or without the ethanol extract in the presence of denture cleanser. Live C. albicans in biofilm was counted by cultured colony forming unit value after inoculated on agar plate. Results: Ethanol extract from shiitake mushroom showed stronger antifungal activity against yeast type of C. albicans compared to its water extract. The ethanol extract significantly reduced count of C. albicans in hyphal biofilm (P < 0.05). Also, the ethanol extract showed synergistically antifungal effect with denture cleanser on candidal biofilm on denture base resin disk (P < 0.05). Conclusion: The ethanol extract of shiitake mushroom may be a candidate for preventing candidal stomatitis as well as denture-related stomatitis.

Bacterial Reverse Mutation Test of Wild Ginseng Culture Extract (산삼배양추출물의 세균을 이용한 복귀돌연변이시험)

  • Song Si-Whan;Yang Deok Chun;Choung Se Young
    • Journal of Food Hygiene and Safety
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    • v.19 no.4
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    • pp.193-197
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    • 2004
  • To evaluate the bacterial reverse mutation of wild ginseng culture extract, the in vitro Ames test using Salmonella typhimurium (TA100, TA1,535, TA98, TA1,537) and Escherichia coli (WP2 uvrA) were performed with wild ginseng extract at the concentrations 0, 1.6, 8, 40, 200, 1,000, 2,500 and $5,000{\mu}g/ml/plate$. Wild ginseng culture extract was negative in Ames test with both Salmonella typhimurium or Escherichia coli with and without rat liver microsomal enzyme (S-9 fraction). According to these results, we concluded that wild ginseng culture extract did not cause bacterial reverse mutation.

Antimutagenicity and Immuno Activity of Extracts from Epimedium koreanum Nakai Containing Different Icariin Quantity (Icariin 함량에 따른 삼지구엽초 추출물의 항돌연변이 및 면역활성)

  • Park, Myoung-Su;Kim, Seo-Jin;Forghani, Fereidoun;Rahman, S.M.E.;Eo, Ji-Hyun;Eun, Jong-Bang;Oh, Deog-Hwan
    • Food Science and Preservation
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    • v.18 no.6
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    • pp.938-945
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    • 2011
  • Epimedium koreanum Nakai is a wild herb commonly consumed in South Korea due to its beneficial health effects. In the present study, the antimutagenic and immunoactivities of extracts from E. koreanum Nakai containing different icariin quantities were investigated for food use. In the Ames test, both the water and ethanol extracts were found not to have a mutagenic effect on Salmonella typhimurium TA98 and TA100, respectively. The E. koreanum Nakai extracts showed over 80 and 90% antimutagenic effects on benzo(${\alpha}$)pyrene (B(a)P) in S. typhimurium TA98 and TA100, respectively. Moreover, all the extracts showed over 70% antimutagenicity on S. typhimurium TA98 and TA100 against 4-nitroquinoline-1-oxide (4NQO). The E. koreanum Nakai extract with ethanol showed strong antimutagenic activity, higher than that of the water extract and the sequenced KE9412, KE9408, and KE9405. In the immunomodulating activity test, the effect of E. koreanum Nakai on the B (Rhamos) and T (Jukat) cells were investigated. The immunoactivity results showed that the growth and viability of the B and T cells increased and were activated more in KE9405 (1.8 times), KE9408 (1.6 times), and KE9412 (1.32 times) in the water extracts, and least in KE9412 (1.74 times), KE9408 (1.52 times), and KE9405 (1.4 times) in the ethanol extracts. In the case of both the water and ethanol extracts ($500{\mu}g/mL$) from E. koreanum Nakai, the highest cell number of the human B (Rhamos) and T (Jukat) cells was observed on day 4 in KE9405 and KE9412, and on day 5 in KE9408. Based on the obtained results, the development of E. koreanum Nakai as a food material is recommended.