Journal of Dental Rehabilitation and Applied Science (구강회복응용과학지)

Korean Academy of Stomatognathic Function and Occlusion (대한턱관절교합학회)
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Preventive effects of shiitake mushroom extract on candida stomatitis

칸디다성 구내염에 대한 표고버섯 추출물의 예방효과

  • Yoo, Hyun-Jun (Department of Preventive Dentistry, College of Dentistry, Dankook University)
  • 유현준 (단국대학교 치과대학 예방치과학교실)
  • Received : 2021.06.09
  • Accepted : 2021.07.20
  • Published : 2021.09.30
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Abstract

Purpose: The purpose of this study was to investigate antifungal activity of shiitake mushroom yeast and hyphal type of Candida albicans. Materials and Methods: The extract from shiitake mushroom was collected by drying the supernatant after soaking shiitake mushrooms in water or ethanol. The antifungal activity of the extracts against yeast type of C. albicans was investigated by the susceptibility assay using microplate. C. albicans biofilm was formed on 12-well plate using Ham's F-12 medium in CO2 incubator and treated with the ethanol extract. Furthermore, C. albicans biofilm was formed on denture base resin disk and treated with or without the ethanol extract in the presence of denture cleanser. Live C. albicans in biofilm was counted by cultured colony forming unit value after inoculated on agar plate. Results: Ethanol extract from shiitake mushroom showed stronger antifungal activity against yeast type of C. albicans compared to its water extract. The ethanol extract significantly reduced count of C. albicans in hyphal biofilm (P < 0.05). Also, the ethanol extract showed synergistically antifungal effect with denture cleanser on candidal biofilm on denture base resin disk (P < 0.05). Conclusion: The ethanol extract of shiitake mushroom may be a candidate for preventing candidal stomatitis as well as denture-related stomatitis.

목적: 본 연구의 목적은 효모형 또는 균사형 Candida albicans에 대한 표고버섯 추출물의항진균효과를 살펴보기 위함이다. 연구 재료 및 방법: 표고버섯 추출물은 표고버섯을 물 또는 에탄올에 닮근 후, 상층액을 건조시켜서 얻었다. 효모형 C. albicans에 대한 추출물의 항진균활성은 마이크로플레이트를 이용한 감수성 시험을 이용하여 조사되었다. C. albicans 생물막을 CO2 배양기에서 Ham's F-12 배지를 이용하여 12-well 플레이트에 형성시키고 에탄올 추출물로 처리하였다. 또한 C. albicans 생물막을 의치상용 레진 디스크에 형성시키고 의치세정제를 에탄올 추출물이 포함 또는 포함되지 않은 조건에서 처리하였다. 두 조건의 항바이오필름 효과 시험에서의 바이오필름내 살아있는 C. albicans를 조사하기 위해서 한천고체배지에 접종한 후 집락 형성 단위(CFU) 값을 측정하였다. 결과: 효모형 C. albicans에 대해서 표고버섯으로부터 물 추출물보다 에탄올 추출물이 강한 항진균력을 보였다. 에탄올 추출물은 균사형 C. albicans 바이오필름에 대해서도 유의적인 항진균력을 보였다(P < 0.05). 또한 에탄올 추출물은 의치세정제와 의치에 형성된 C. albicans 바이오필름에 대한 항진균력에 대해서 동반상승효과를 보였다(P < 0.05). 결론: 표고버섯 에탄올 추출물은 구강 칸디다증 예방뿐만 아니라 의치관련 구내염에 대해서 예방할 수 있는 후보물질로 사료된다.

Keywords

Acknowledgement

The present research was supported by a research fund of Dankook University in 2020

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