• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.6
• /
• pp.898-903
• /
• 2010

This study was carried out to estimate carbon emission using LCA (Life Cycle Assessment) and to establish LCI (Life Cycle Inventory) database of soybean production system. Based on collecting the data for operating LCI, it was shown that input of organic fertilizer was value of 3.10E+00 kg $kg^{-1}$ soybean and it of mineral fertilizer was 4.57E-01 kg $kg^{-1}$ soybean for soybean cultivation. It was the highest value among input for soybean production. And direct field emission was 1.48E-01 kg $kg^{-1}$ soybean during soybean cropping. The result of LCI analysis focussed on greenhouse gas (GHG) was showed that carbon footprint was 3.36E+00 kg $CO_2$-eq $kg^{-1}$ soybean. Especially $CO_2$ for 71% of the GHG emission. Also of the GHG emission $CH_4$, and $N_2O$ were estimated to be 18% and 11%, respectively. It might be due to emit from mainly fertilizer production (92%) and soybean cultivation (7%) for soybean production system. $N_2O$ was emitted from soybean cropping for 67% of the GHG emission. In $CO_2$-eq. value, $CO_2$ and $N_2O$ were 2.36E+00 kg $CO_2$-eq. $kg^{-1}$ soybean and 3.50E-01 kg $CO_2$-eq. $kg^{-1}$ soybean, respectively. With LCIA (Life Cycle Impact Assessment) for soybean production system, it was observed that the process of fertilizer production might be contributed to approximately 90% of GWP (global warming potential). Characterization value of GWP was 3.36E+00 kg $CO_2$-eq $kg^{-1}$.

• Korean Journal of Breeding Science
• /
• v.42 no.6
• /
• pp.679-683
• /
• 2010

'Yeonjami' is a new sweetpotato variety developed for table use by Bioenergy Crop Research Center, National Institute of Crop Science (NICS), RDA in 2008. This variety was selected from the cross between 'Ayamurasakki' and 'Poly Cross' in 2000, and seedling and line selections were practiced from 2001 to 2003. Preliminary and advanced yield trials were carried out from 2004 to 2005. The regional yield trials were conducted at six locations from 2006 to 2008. 'Yeonjami' has cordate leaf, green vine and petiole, long elliptic storage root, purple skin and light purple flesh color of storage root. The average yield of storage root was 25.9 ton/ha in the regional yield trials, which was 15% higher than that of 'Sinjami' variety. Number of storage roots over 50 gram per plant was 2.9, and the average weight of one storage root was 153 gram. This variety was partly-resistant to Fusarium wilt and nematode. In addition, steamed 'Yeonjami' has higher polyphenol contents as 139.6 mg/100 g and higher biological activities as, which may improve the bioactivity in human.

• Korean Journal of Heritage: History & Science
• /
• v.55 no.1
• /
• pp.305-321
• /
• 2022

The stone chamber tomb in Eungpyeong-ri, Buyeo, is a joint tomb that contains the bodies of two individuals. This paper investigates the relationship between the buried persons and the characteristics of the stone chamber tomb. Based on the geographical location, relics, and the excavated human bones, it was determined that the tomb was built during the Sabi Period of the Baekje Dynasty and that the buried individuals were most probably residents of high stature or government officials. To study the excavated bones, the remains were carefully collected and conservation was carried out. Before collecting samples from the human bones for the analytical research, the results of near-infrared analysis were used to collect the samples for the isotope analysis and DNA analysis. The most important issue when handling the excavation site was the reinforcing agent and the concentration of the agent used. In situations like this, Paraloid B-72 is the most suitable agent. When the shape of human bones was difficult to distinguish from the soil, conservation was performed using X-ray and CT imaging data. The same chemical used for the reinforcement of the site was used to complete a minimum level of conservation to the surface areas where the conservation treatment of removing foreign substances, the reinforcement areas, and bonded areas were carried out. The collagen yield from the sample obtained at selected position was 3.8% to 6.1%. The results of analyzing the stable isotopes of carbon and nitrogen found in the extracted collagen showed that the stable isotope ratios came out to δ¹³C -18.3‰±0.1‰, -19.0‰±0.1‰ for EBW and δ¹⁵N 10.7‰±0.5‰, 10.6‰±0.1‰ for EBE. It is believed the two individuals consumed small amounts of minor cereals, mainly from C₃ plants, and protein was obtained from eating terrestrial animals. What's more, the deviations in data obtained from the two individuals were so small that it could be inferred that the individuals ate similar foods. Considering the preservation state of the sample, amplifying DNA for the DNA analysis would have been very difficult since the amount of surviving DNA was so deficient. For DNA analysis, it is anticipated that the results could be derived by applying improved extraction methods that will be developed in the future. In this research, any association between scientific analysis(DNA and stable isotope ratio) and near-infrared spectroscopy was difficult to establish. Further research is needed on the utilization of near-infrared analysis for gathering samples from human bones.

• Journal of Applied Biological Chemistry
• /
• v.65 no.4
• /
• pp.421-427
• /
• 2022

In this study, Jeju Tatary buckwheat tea's chemical composition and physiological activities were compared according to the leaching temperature (60, 80, 100 ℃). As the leaching temperature is increased, the degree of browning is induced. However, there was no significant change in pH. The total polyphenol content was higher at 80 ℃ than at 60 ℃ leaching temperature, but significantly decreased at 100 ℃ leaching temperature (60 ℃: 17.06 mg GA/g, 80 ℃: 20.09 mg GA/g, 100 ℃ :18.45 mg GA/g). There were high content of flavonoid and rutin as the leaching temperature increased. Consistently, 2,2-diphenyl1-picrylhydrazyl (DPPH) radical scavenging activity and tyrosinase inhibitory activity were significantly higher with increasing temperature (DPPH % inhibition: 60 ℃: 41.88%, 80 ℃: 46.01%, 100 ℃: 46.80%/tyrosinase inhibitory activity: 60 ℃: 9.38%, 80 ℃: 22.94%, 100 ℃: 28.17%). However, there was no significant difference in DPPH radical scavenging activity between 80 and 100 ℃. A cytotoxicity test was performed by treating with Jeju Tatary buckwheat extract into mouse macrophage cells (Raw264.7). 100 and 200 ㎍/mL treatment (100 ℃ extract) were significantly upregulated the survival rate, but there was no significant difference in other concentrations. Collectively, most of the bioactive components, antioxidant activity, and tyrosinase inhibitory activity were induced as the leaching temperature increased. However, the content of polyphenols which are known to have antioxidant activity, was significantly reduced at 100 ℃ leaching temperature. Several reports have demonstrated that leaching at too high temperature lowered the overall acceptability, so the optimal leaching condition of Tatary Buckwheat is 80 ℃, 5 min in this study.

• Animal Systematics, Evolution and Diversity
• /
• v.11 no.1
• /
• pp.39-64
• /
• 1995

A faunistic and ecological study on the occurrence of freshwater cladocerans and copepods was accomplished from Chindo, South Korea. Collections were made from total 35 stations, comprising the various freshwater habitats like reservoirs, streams, swamps, bogs, ricefields, ditch, pond, and spring during the periods of July 23-25, and November 1-3 in 1994. Twenty seven cladoceran species of 17 genera of 6 families in 2 orders, and 28 copepod species of 21 genera of 6 families in 3 orders were collected during this research period, of which Daphnia obtusa Kurz and Elaphoidella bidens (Schmeil) are newly recorded from Korea. In reservoirs, Diaphanosoma sp. and Thermocyclops taihokuensis were dominant in July, and then succeeded by Bosmina longirostris and Cyclops vicinus vicinus in November. Thermocyclops crassus co-occurred with 7: taihokuensis at both seasons, was frequent in November after T. taihokuensis precipitately decreased. In other stagnant waters, 7: taihokuensis and Moina weismanni were dominant at ponds in July and in November, respectively. At ricefields in July Moina macrocopa and T. taihokuensis were dominant, but in November M. macrocopa and Paracyclops fimbriatus were. At streams, cladocerans were relatively rare, but became more rich in November. The representative cladoceran species were Bosmina longirostris as a plankton, and Chydorus sphaericus as a epibenthic species. Concerning copepods, nearly all the stations of streams except a few ones adjacent to seashore showed the similiar species constitutions, of which E. serrulatus and M, pehpeiensis were most frequent and abundant. At a mountain streamlet and a spring, the occurrence of Alona sp., Attheyella byblis Chang and Kim, 1992 and A. tetraspinosa Chang, 1993 is quite interesting and deserved much attention in the taxonomical point of view. Seventeen major cladocerans and copepods from lentic habitats and 13 major cladocerans and copepods from lotic habitatats were clustered using average taxonomic distance and UPGMA to infer the co-occurrence relations among species. As for lentic habitats, two large phena were appeared at first. The one phenon consisted of Diaphanosoma sp. and T taihokuensis, and showed its predominancy over the various habitats and its dominancy was rapidly decreased in November. The other phenon frequently occurred rather in November, and subdivided into three subgroups. On the other hand, as for lotic habitats, 13 species were also grouped into 2 large phena. The first one comprised 4 species, which were dominant and highly frequent at nearly all the lotic habitats, and subdivided into three subgroups according to their seasonal fluctuation types. The second one was also subdivided into three phena, the first of which comprised only one species, Microcyclops varicans, and occurred at most of the stations along stream with steadiness through the research period; the second phenon, Chydorus sphaericus, occurred much frequently in November; the last phenon included a few heterogenous subgroups.

• Tuberculosis and Respiratory Diseases
• /
• v.43 no.1
• /
• pp.63-68
• /
• 1996

Background: Interferon-$\gamma$ has various biologic effects, including antiviral effect, antitumor proliferative effect, activation of macrophage and B lymphocyte, and increased expression of major histocompatibility complex. Especially, antitumor proliferative effect of interferon-$\gamma$ has already been proved to be important in vivo as well as in vitro. And, clinical studies of interferon-$\gamma$ have been tried in lung cancer patients. However, the mechanism of antitumor effect of interferon-$\gamma$ has not yet been established despite of many hypotheses. "Necrosis" is a type of cell death which is well known to occur in the circumstances of severe stresses. In contrast, "apoptosis" is another type of cell death which occurs in such biological circumstances as embryonic development, regression of organs, and self-tolerance of lymphocytes. And, apoptosis is an active process of cell death in which cells are dying with fragmentations of their cytoplasms and nuclei. And, in the process of apoptosis the DNAs of cells are cleaved between nucleosomes by unidentified endonuclease and therefore DNAs of apoptotic cells result in a typical electrophoresis pattern known as DNA ladder pattern. Recently it has been suggested that cytotoxic effect of interferon-$\gamma$ occurs via apoptosis. To elucidate the mechanism of antitumor cytotoxic effect of interferon-$\gamma$, we microscopically observed a lung cancer cell line, A549 which was treated with interferon-$\gamma$. We observed A545 treated with interferon-$\gamma$ was dying fragmented. And so, we performed this study to find out that the mechanism of antitumor cytotoxic effect of interferon-$\gamma$ be apoptosis. Method: We treated A549, human lung cancer cell line with various concentration of interferon-$\gamma$ and quantified its cytotoxic effect of various periods, 24 hours, 72 hours and, 120 hours by MTT(dimethylthiazolyl diphenyltetrazolium bromide) bioassay. Also, after we treated A549 with 100 units/mi of interferon-$\gamma$ for 120 hours, we observed the pattern of cell death with inverted microscope and we extracted DNAs from the dead A549 cells and observed the pattern of 1.5% agarose gel electrophoresis with ethidium bromide staining. Result: 1) Cytotoxic effect of interferon-$\gamma$ on A549: For the first 24 hours, threre was little cytotoxic effect and for between 24 hours and 72 hours, there was the beginning of cytotoxic effect and for 120 hours there was increased cytotoxic effect. 2) Pattern of A549 cell death by interferon-$\gamma$: We observed with inverted microscope that A549 cells were dying fragmented. 3) DNA ladder pattern of gel electrophoresis: We observed DNA ladder pattern of gel electrophoresis of extracted DNAs from dead A549 cells. Conclusion: We concluded that the mechanism of interferon-$\gamma$induced cytotoxicity on lung cancer cell line, A549 be via apoptosis.

• Tuberculosis and Respiratory Diseases
• /
• v.49 no.3
• /
• pp.310-322
• /
• 2000

Background : Phospholipase C(PLC) plays an important role in cellular signal transduction and is thought to be critical in cellular growth, differentiation and transformation of certain malignancies. Two second messengers produced from the enzymatic action of PLC are diacylglycerol (DAG) and inositol 1, 4, 5-trisphosphate (IP3). These two second messengers are important in down stream signal activation of protein kinase C and intracellular calcium elevation. In addition, functional domains of the PLC isozymes, such as Src homology 2 (SH2) domain, Src homology 3 (SH3) domain, and pleckstrin homology (PH) domain play crucial roles in protein translocation, lipid membrane modificailon and intracellular memrane trafficking which occur during various mitogenic processes. We have previously reported the presence of PLC-${\gamma}1$, ${\gamma}2$, ${\beta}1$, ${\beta}3$, and ${\delta}1$ isozymes in normal human lung tissue and tyrosine-kinase-independent activation of phospholipase C-${\gamma}$ isozymes by tau protein and AHNAK. We had also found that the expression of AHNAK protein was markedly increased in various mstologic types of lung can∞r tissues as compared to the normallungs. However, the report concerning expression of various PLC isozymes in lung canærs and other lung diseases is lacking. Therefore, in this study we examined the expression of PLC isozymes in the paired surgical specimens taken from lung cancer patients. Methods : Surgically resected lung cancer tissue samples taken from thirty seven patients and their paired normal control lungs from the same patients, The expression of various PLC isozymes were studied. Western blot analysis of the tissue extracts for the PLC isozymes and immunohistochemistry was performed on typical samples for localization of the isozyme. Results : In 16 of 18 squamous cell carcinomas, the expression of PLC-${\gamma}1$ was increased. PLC-${\gamma}1$ was also found to be increased in all of 15 adenocarcinoma patients. In most of the non-small cell lung cancer tissues we had examined, expression of PLC-${\delta}1$ was decreased. However, the expression of PLC-${\delta}1$ was markedly increased in 3 adenocarcinomas and 3 squamous carcinomas. Although the numbers were small, in all 4 cases of small cell lung cancer tissues, the expression of PLC-${\delta}1$ was nearly absent. Conclusion : We found increased expression of PLC-${\gamma}1$ isozyme in lung cancer tissues. Results of this study, taken together with our earlier findings of AHNAK protein-a putative PLD-${\gamma}$, activator-over-expression, and the changes observed in PLC-${\delta}1$ in primary human lung cancers may provide a possible insight into the derranged calcium-inositol signaling pathways leading to the lung malignancies.

• Journal of Nutrition and Health
• /
• v.49 no.5
• /
• pp.347-357
• /
• 2016

Purpose: This study investigated bone mineral density and nutritional state according to consumption of milk in Korean postmenopausal women who drink coffee. Methods: Using the 2008~2009 Korean National Health & Nutrition Examination Survey data, a total of 1,373 postmenopausal females aged 50 yrs and over were analyzed after excluding those with diseases related to bone health. According to coffee and/or milk consumption, subjects were divided into four groups: coffee only, both coffee & milk, milk only, and none of the above. All data were processed after application of weighted values and adjustment of age, body mass index, physical activity, drinking, and smoking using a general linear model. For analysis of nutrient intake and bone density, data were additionally adjusted by total energy and calcium intake. Results: The coffee & milk group had more subjects younger than 65 yrs and higher education, urban residents, and higher income than any other group. The coffee only group showed somewhat similar characteristics as the none of the above group, which showed the highest percentage of subjects older than 65 and in a lower education and socio-economic state. Body weight, height, body mass index, and lean mass were the highest in coffee & milk group and lowest in the none of the above group. On the other hand, the milk only group showed the lowest values for body mass index and waist circumference, whereas percent body fat did not show any difference among the groups. The coffee and milk group showed the highest bone mineral density in the total femur and lumbar spine as well as the highest nutritional state and most food group intakes, followed by the milk only group, coffee only group, and none of the above group. In the assessment of osteoporosis based on T-score of bone mineral density, although not significant, the coffee and milk group and milk only group, which showed a better nutritional state, included more subjects with a normal bone density, whereas the none of the above group included more subjects with osteoporosis than any other group. Conclusion: Bone mineral density in postmenopausal women might not be affected by coffee drinking if their diets are accompanied by balanced food and nutrient intake including milk.

• Journal of the Korean Vacuum Society
• /
• v.15 no.6
• /
• pp.563-575
• /
• 2006

Ti(C,N) films are synthesized by pulsed DC plasma enhanced chemical vapor deposition (PEMOCVD) using metal-organic compounds of tetrakis diethylamide titanium at $200-300^{\circ}C$. To compare plasma parameter, in this study, $H_2$ and $He/H_2$ gases are used as carrier gas. The effect of $N_2\;and\;NH_3$ gases as reactive gas is also evaluated in reduction of C content of the films. Radical formation and ionization behaviors in plasma are analyzed in-situ by optical emission spectroscopy (OES) at various pulsed bias voltages and gas species. He and $H_2$ mixture is very effective in enhancing ionization of radicals, especially for the $N_2$. Ammonia $(NH_3)$ gas also highly reduces the formation of CN radical, thereby decreasing C content of Ti(C, N) films in a great deal. The microhardness of film is obtained to be $1,250\;Hk_{0.01}\;to\;1,760\;Hk_{0.01}$ depending on gas species and bias voltage. Higher hardness can be obtained under the conditions of $H_2\;and\;N_2$ gases as well as bias voltage of 600 V. Hf(C, N) films were also obtained by pulsed DC PEMOCYB from tetrakis diethyl-amide hafnium and $N_2/He-H_2$ mixture. The depositions were carried out at temperature of below $300^{\circ}C$, total chamber pressure of 1 Torr and varying the deposition parameters. Influences of the nitrogen contents in the plasma decreased the growth rate and attributed to amorphous components, to the high carbon content of the film. In XRD analysis the domain lattice plain was (111) direction and the maximum microhardness was observed to be $2,460\;Hk_{0.025}$ for a Hf(C,N) film grown under -600 V and 0.1 flow rate of nitrogen. The optical emission spectra measured during PEMOCVD processes of Hf(C, N) film growth were also discussed. $N_2,\;N_2^+$, H, He, CH, CN radicals and metal species(Hf) were detected and CH, CN radicals that make an important role of total PEMOCVD process increased carbon content.

• Korean Journal of Community Nutrition
• /
• v.26 no.3
• /
• pp.188-199
• /
• 2021

Objectives: The purpose was to identify the ingredients that are usually surveyed for assessing real prices and to present the demand for such surveys by nutrition teachers and dietitians for ingredients used by school foodservice. Methods: A survey was conducted online from December 2019 to January 2020. The survey questionnaire was distributed to 1,158 nutrition teachers and dietitians from elementary, middle, and high schools nationwide, and 439 (37.9% return rate) of the 1,158 were collected and used for data analysis. Results: The ingredients which were investigated for price realities directly by schools were industrial products in 228 schools (51.8%), fruits in 169 schools (38.4%), and specialty crops in 166 schools (37.7%). Moreover, nutrition teachers and dietitians in elementary, middle, and high schools searched in different ways for the real prices of ingredients. In elementary schools, there was a high demand for price information about grains, vegetables or root and tuber crops, special crops, fruits, eggs, fishes, and organic and locally grown ingredients by the School Foodservice Support Centers. Real price information about meats, industrial products, and pickled processed products were sought from the external specialized institutions. In addition, nutrition teachers and dietitians in middle and high schools wanted to obtain prices of all of the ingredients from the Offices of Education or the District Office of Education. Conclusions: Schools want to efficiently use the time or money spent on research for the real prices of ingredients through reputable organizations or to co-work with other nutrition teachers and dietitians. The results of this study will be useful in understanding the current status of the surveys carried out to determine the real price information for ingredients used by the school foodservice.