• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.448-453
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• 2009

This study was performed to elucidate anticancer activities of various parts, such as peel, flesh, placenta, seed, stalk and stem leaf of oriental melon. Chemopreventive and anticancer effects of oriental melon extract were evaluated by detoxifying enzyme, quinone reductase (QR) inductive activity, cytotoxicity and growth inhibitory effect against hepatoma cell. Stalk and stem leaf extracts of oriental melon showed the increment of QR inductive activity with dose-dependent manner and induced quinone reductase 3.9, 1.5-fold at $200{\mu}g/mL$ respectively compared to control. The growth inhibitory effect of oriental melon extract against mouse hepatoma cell (Hepa1c1c7) was investigated by crystal violet (CV) assay. Stalk and stem leaf of oriental melon showed potent growth inhibitory effect. Based on these result, the growth inhibitory effects of stalk, stem leaf at various concentration were examined in detail by MTT assay using human hepatoma cancer cell (HepG2). All of two parts showed growth inhibitory effects and expecially stalk exhibited inhibitory effect of 60.3% at maximum concentration. The above results suggest that stalk of oriental melon has a possibility as a source of natural cancer chemopreventive materials.

• Korean Journal of Plant Resources
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• v.26 no.5
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• pp.652-657
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• 2013

In the present study, anti-oxidative and the RMC proliferation inhibitory propeties of 80% ethanol extracts from 63 kinds of traditional medicines were investigated. Inhibitory effects of RMC proliferation were showed that Dalbergia odorifera T. Chen., Melia azedarach Linn$\acute{e}$ and Hydnocarpus anthelmintica Pierre. Among them Hydnocarpus anthelmintica Pierre had the highest anti-oxidative activity ($ORAC_{PE\;value}=1.6$, DPPH = 81.1), but Dalbergia odorifera T. Chen. and Melia azedarach Linn$\acute{e}$ had no effects. These results suggest that the Hydnocarpus anthelmintica Pierre could prevent or protect from kidney disease as antioxidant and anti-proliferative agent for RMC.

• Journal of Dairy Science and Biotechnology
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• v.15 no.1
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• pp.33-44
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• 1997

We investigated the immunological function of cheese whey protein concentrate (CWPC), which is a by-product of cheese production, using mitogenic activity in murine splenocytes as an index. A fraction isolated by gel filtration and anion exchange chromatography of CWPC showed high mitogenic activity, comparable to the activity of lipopolysaccharide (LPS). The fraction was detected as a single band on SDS-PAGE. It contained calcium, inorganic phosphorus, and carbo-hydrate, indicating the active component to be a glycophosphopeptide (GPP) Since pronase digestion of GPP did not reduce its mitogenic activity, carbohydrate rather than peptide may be important in the activity, When applied on an anti-${\beta}$-caseinophosphopeptide (${\beta}$-CPP ) antibody affinity column, the GPP was separated into two components, one with affinity to ${\beta}$-CPP and the other without such affinity. Both the components contained N-linked oligosaccharide chains and had the mitogenic activity. These results demonstrate that cheese whey contains a GPP having strong mitogenic activity

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.2
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• pp.217-222
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• 2003

We studied effects of hot water extract of Lentinus edodes (Berk.)sing. and Pleurotus eryngii (De Candolle ex Fries) Quel mushroom on proliferation and apoptosis of the human colon adenocarcinoma, HT-29 and Caco-2.. Cells were maintained with Dulbecco's modified Eagle medium/Ham's F-12 nutrient mixture supplemented with 10% fetal bovine serum at 37$^{\circ}C$ in a humidified $CO_2$. For cell proliferation experiments, cells were seeded in 35 mm dishes, treated with the various concentrations of the extract for the different time course. Apoptosis was measured by caspase-3 activity The more contents of the extract added in HT-29 and Caco-2 were, the more cell proliferation was suppressed. When we incubated HT-29 cells for 24, B\ulcorner72, and 96 hours after treatments, cell proliferation was markedly suppressed after 96 hours. Also, caspase-3 activity in HT-29 was increased by the treatment of Lentinus edodes and Pleurotus eryngii extracts. However, the treatment of the extract to SNU484, Korean stomach adenocarcinoma, did not show any influence on cell proliferation and caspase-3 activity Therefore, Lentinus edodes and Pleurotus eryngii are strongly recommended for the prevention and treatment of colon cancer.

• Journal of Food Hygiene and Safety
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• v.14 no.3
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• pp.270-276
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• 1999

In previous reports, the authors isolated the algicidal marine bacterium, Alteromonas sp. SR-14 and demonstrated its growth inhibition of diatom, Chaetoceros calcitrans (C. calcitrans). In this paper, we studied the effects of cell free culture filtrate of Alteromonas sp. SR-14 on the growth of C. calcitrans, and the characteristics of the algal growth inhibition substance. The culture filtrate of Alteromonas sp. SR-14 grown in peptone broth showed growth inhibition activity against C. calcitrans. The reasonable culture conditions of the bacterium for producing of algal growth inhibition substances were $15~20^{\circ}$ in temperature, 7.0-9.0 in pH and $23~30{\textperthousand}$ in salinity, respectively. The algal growth inhibition activity of culture filtrate was increased from stationary phase in growth curve of Alteromonas sp. SR-14. The molecular weights of algal growth inhibition substances produced by Alteromonas sp. SR-14 were ranged about from 3 KDa to 12 KDa. Among the substances, less than 10 KDa fraction were stable by heating at $100^{\circ}$ for 10 minutes, while more than 10 KDa fraction were heat labile. According to the experimental results, the algal growth inhibition substance produced by the bacterium was not a single compound.

• Korean Journal of Food Science and Technology
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• v.34 no.4
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• pp.688-693
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• 2002

The anticarcinogenic effects of various food components have received much attention in recent years. However mechanism of anticarcinogens in food materials on cancer cells have rarely been investigated. This study was performed to investigate the effects on the cytotoxicity and quinone reductase (QR) activity of Allium tuberusum (AT) on the human cancer cells. The six partition layers which are methanol (ATM), hexane (ATMH), ethylether (ATMEE), ethylacetate (ATMEA), butaonl (ATMB) and aqueous (ATMA) of Allium tuberusum were screened for their cytotoxic effects on HepG2, MCF-7, HeLa and SK-N-MC cells by the MTT assay. Among the six partition layers, ATMEE had the strongest cytotoxic effect at concentration of $150\;{\mu}g/mL$ which resulted over 95% on HepG2, HeLa, MCF-7 and SK-N-MC cell lines. The ATMEA also showed significant cytotoxic effect on HepG2 and SK-N-MC cell lines. The ATMB showed the highest induction activity of QR on HepG2 cells among the other partition layers. QR activity of HepG2 cells, grown in the presence of ATMB at the concentration of $50\;{\mu}g/mL$, was increased by 3.9 times, compared to the control value of 1.0. Based on these results, the ATMEE and ATMB may have potentially anticarcinogenic and chemopreventive activities.

• Food Industry And Nutrition
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• v.8 no.1
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• pp.50-56
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• 2003

팽이버섯 균사체를 액체배양하여 전통 장류 발효식품및 음료개발에 이용할 목적으로 7종류의 곡물에 배양한 팽이버섯 배양액의 생리활성을 검토하였다 혈전 용해능은 골목 배양액과 침전물사이에는 차이가 없었으며, 대체로 조, 대두박 및 검정콩 배지에서 혈전용해능이 높게 나타났다. 항균력은 S.aureus에 대해서는 밀, 보리 및 검정콩이 높았고, L. plantarum에 대해서는 조, 밀 및 보리가 높았으며, E.coli에 대해서는 밀과 보리 배지에서 배양한 배양액이 높게 나타났다. Linoleic acid에 대한 항산화 효과는 곡물배지 자체에서는 검정콩, 대두박 및 합성배지에서 높게 나타났고, 팽이버섯을 배양했을 때는 검정콩배지의 항산화력과 유사한 활성을 나타내었다. 비장세포의 증식능은 보리, 밀, 조 및 합성배지에 팽이버섯을 배양하였을 때가 곡물자체에서보다 약 20% 정도 높게 나타났다. ConA를 버섯배양 추출물에 혼합했을 때는 옥수수, 대두박 및 검정콩에서 팽이버섯을 배양한 배양액이 $22\sim26%$ 정도의 증식효과가 있었으며, LPS를 혼합했을 경우는 옥수수, 대두박, 검정콩배지에 배양한 배양액이 각각 45%, 25%,18%의 증식효과가 나타내었다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.4
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• pp.415-424
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• 2020

In the present study, we have refined gomisin N, which represents activity in the proliferation of dermal papilla cells (HFDPCs) from the fruit of Schisandra chinensis (S. chinensis), and have identified optimal extraction conditions for obtaining extracts with high content of gomisin N. The activity of the extracts and fractions was evaluated, and the results indicated approximately 29% proliferation activity in the group treated with 1 ㎍/mL of n-hexane fraction. Column chromatography was used to assess the active ingredient in the n-hexane fraction, and two compounds, namely gomisin N(1) and schisandrin(2), were isolated and identified. When the HFDPCs proliferation activity was tested for the isolated compounds, gomisin N exhibited ≥ 20% proliferation activity. Thus, via response surface methodology (RSM), the optimum extraction conditions to obtain the maximum level of gomisin N from the fruit of S. chinensis were determined, where ethanol proportion, extraction time, and extraction temperature were used as the independent variables. The results revealed coefficient of determination ≥ 0.95 and p-value ≤ 0.05, which confirmed the fit of the model. The optimum extraction conditions to achieve the maximum content of gomisin N were as follows: ethanol proportion 83.8%, extraction temperature 80 ℃, and extraction time 8.7 h. The content of gomisin N using these conditions was predicted as 378,300 ppm, and a mean value close to the predicted value (376,884 ppm) was obtained while validating the aforementioned conditions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1214-1220
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• 2003

Antimicrobial activity of Rhus javanica (RJ) extract against animal husbandry disease-related bacteria was determined by a paper disc method. The RJ extracts showed a significant antimicrobial activity against Gram positive (+) bacteria and especially the activity was most potent against L. monocytogenes and S. epidermidis. Minimum inhibitory concentrations (MIC) of the MeOH and EtOH extracts of RJ were in the range of 0.8 ∼ 16 mg/mL and 0.8 ∼ 10 mg/mL, respectively. Furthermore, among five solvent fractions (n-hexane, CHC1$_3$, EtOAc, n-BuOH and $H_2O$ frs.) from MeOH extract of RJ, the EtOAc fr. exhibited the most significant antimicrobial activity The antimicrobial activities of RJ extracts against most microbial strains were unstable by either heat treatment or acid treatment. The inhibitory effect of RJ extracts on microbial cell growth was further examined by the addition of 0, 100, 300, and 500 ppm of RJ extracts into growth medium. The growth of gram positive (+) bacteria, S. aureus, S. epidermidis and L. monocytogenes was inhibited for 72 hours when at least 300 ppm of RJ extracts added, but the growth of gram negative (-) bacteria was only inhibited when at least 500 ppm of RJ extracts were added. Taken together, tile antimicrobial activities of RJ extracts were more effective against gram positive (+) bacteria compared to those against gram negative (-) bacteria.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.430-434
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• 2001

The algicidal activity of Shewanella (formerly Alteromonas) sp. SR-14 against diatom, Chaetoceros calcitrans was reported in our previous papers. In this study, the effect of Vibrio alginolyticus on the algicidal activity of Shewanelia sp, SR-14 was examined under the optimum algicidal conditions, i.e., temperature ($21\pm1^{\circ}C$), light intensity (4,000 lux), and light: dark cycle (12 hour: 12 hour). Shewanella sp. SR-14 grew well in the presence or the absence of V. alginolyticus in Conwy medium. Algal growth was only inhibited by Shewanella sp. SR-14. V. alginolyticus did not show the algicidal activity, Growth of C. calcitrans increased synergistically with growth of V. alginolyticus. When the initial inoculum of V. alginolyticus was only 1 log cycle higher than that of Shewanella sp. SR-14, the effect of V. alginolyticus on the algicidal activity of Shewanella sp. SR-14 was insignificant during incubation of mixed culture, i.e., two bacterial species and the alga. However, when V. alginolyticus dominated Shewanella sp. SR-14 by 3 log cycles of bacterial counts, it was found that the strain SR-14 could not inhibited growth of C. calcitrans up to 5 days of incubation.