• Applied Microscopy
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• v.32 no.4
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• pp.319-327
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• 2002

Salvinia natans, an unique water fern having a small rootless body, developed three different types of trichomes throughout the plant. The most peculiar type exhibiting rows of obvious, whitish, multicellular trichome clusters was noticed in the upper surface of the floating leaves. Eight to ten branches within a cluster extended ca. $370{\sim}420{\mu}m$ from the leaf surface. No stalk cell was found, however, four large epidermal cells were discernable at the base of four central branches in the cluster. Each branch consisted of $8{\sim}10$ obliquely-oriented small cells that gradually decreased in size toward the branch tip. The second type was found in the lower surface of the floating leaves, stems, and sporocarps. Multicellular uniseriate trichomes, ca. $430{\sim}980{\mu}m$ long, were distributed all over these structures. The tip of trichome was acicular, but a semi-spheric protuberance of approximately $24{\sim}32{\mu}m$ in diameter occurred at the base of each trichome. The protuberance appeared to be firmly attached to the side of the basal cell, however, internal connection to the trichome cell itself was uncertain. The third type was similar to the second in that multicellur uniseriate trichomes with acicular tip and a protuberance at the base were present. However, the trichomes were considerably long relative to the second type, and only occurred along the surface of highly dissected, submerged leaves. A majority of the trichomes exceeded more than 2 mm in length that hung downward in the water. Regardless of trichome type, all trichomes contained a huge central vacuole with very thin cytoplasm, resulting from the fusion of several vacuoles during early trichome development. The various densely-distributed trichomes formed in Salvinia natans probably play an important role in plant buoyancy.

• Korean Journal of Medicinal Crop Science
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• v.5 no.3
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• pp.206-210
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• 1997

The pathogenic fungi associated with blight of leaf and stem in peony were leaf spot (Alternaria sp.), powdery mildew (Erysiphe aquilegiae) and rust (Cronartium flaccidum). The infection of leaf spot and powdery mildew begins from late April to midMay and rust was infected in early June. Blight time of aerial part in peony started from late May and the ratio of blight on leaf and stem was more than 50% in late Aug. Yields of root by the incidence time of blight of leaf and stem were 69.1% in late June, 65.4% in late July and $87.6{\sim}92.7$% in August and September. The number of root of more than 10mm in root diameter blighted in late June and July was much lower than in August, but the paeoniflorin content in the former was much higher than the latter.

• Journal of Bio-Environment Control
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• v.6 no.3
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• pp.151-158
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• 1997

Tomato (lycopersicon esculentum Mill.‘Sunroad’) seedlings grafted onto the four different rootstocks, ‘Anchor-T’, ‘Kagemushia’, ‘Joint’ and ‘Vulcan’, and fed with different amount of nitrogen were investigated for their susceptibility to the root rot Fusarium wilt (Fusarium oxysporum Schl. f. sp. lycopersici Snyder et Hansen, race J$_3$) disease, plant growth, and fruit quality. The flowering of the first flower cluster was promoted in grafted plants (GP) as compared to non-grafted (NG) ones. The plant height was increased by the grafting, and the growth and stem diameter were enhanced with increased nitrogen levels. Grafted plants were little affected by the Fusarium wilt disease except ‘Anchor-T’rootstock (64.7％ infection). The rate of malformed, underdeveloped, and gray-mold infected fruits were reduced in the grafted plants regardless of the rootstocks. Soluble solid content, sugars, ascorbic acids and organic acids did not show any difference between grafted and non grafted plants, but ascorbic acid tends to be reduced in the high nitrogen treatment. Content of N, Ca and Mg in leaves were greatly increased in the GP grown with high nitrogen level (30kg.㏊$^{-1}$ ).

• Horticultural Science & Technology
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• v.28 no.4
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• pp.580-584
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• 2010

The effect of planting densities on the growth of $Sedum$ $samentosum$ (4 collections) was investigated using nutrient film technique (NFT) with Yamazaki' lettuce nutrient solution (1982) from September 5th to October 24th in non-heating plastic film house. At 40 days, the plant height of 4 collections showed the range of 15.0-18.9 cm, and mean plant height was high the order of $10{\times}10$, $2.5{\times}2.5$, and $5{\times}5cm$. The growth of 'Pohang' and 'Wando' collections was better compared to that of 'Gunsan' and 'Wanju' collections. Number of node, stem diameter, and fresh and dry weight per plant were decreased in higher planting density. Despite the lower fresh weight per plant obtained, the fresh yield per $m^2$ was significantly increased in higher planting density. The mean fresh yield was $14.9kg{\cdot}m^{-2}$ in $2.5{\times}2.5cm$, and 'Pohang' collection showed the highest fresh yield ($17.6kg{\cdot}m^{-2}$). The first optimized harvesting time base on plant height was 30-40 days after NFT culture during autumn season. In eating quality, compression force of stem and bitterness of shoot were decreased in higher planting density. However, the heavy labor demanding high density cutting needs to be improved for hydroponic culture of $S.$ $samentosum$.

• Korean Journal of Environment and Ecology
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• v.18 no.3
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• pp.316-325
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• 2004

This study was carried out to survey the usability of Vitex rotundifolia as an afforestation and landscaping plant on destroyed slopes, sandyhills of sea boards, and places in need of landscaping. The growth characteristics of seedlings and rooted cuttings of Vitex rotundifolia in soils of varying properties and water content ere studied. In three test soils, with a water content of 5% each, the survival rate was more than 70ole for seedlings and more than 40％ for rooted cuttings. This demonstrates that Vitex rotundifolia is a very strongly xeric tree species. The length of stem, the diameter of root stock, leaves, and the length of root increased in all the three types of soil as the quantity of soil water increased. The difference of the growth in response to the water content of the soil was most pronounced in the length of the stem. The growth of seedlings was most notable in the weathered granitic soil. The sea sand and the red silt loam came second and third, respectively. However, there was no major difference in the three test soils. Thus, Vitex rotundifolia appears well-suited to several soil properties and soil water quantities, which makes it very useful as an afforestation and landscaping plant in various sites.

• Journal of Korean Society of Forest Science
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• v.89 no.1
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• pp.85-92
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• 2000

Four Chamaecyparis obtusa plantations of 6, 9, 14, and 20 years were studied to investigate biomass, net production and nutrient distribution. There was wide difference in DBH and height growth of the stands according to site and soil conditions. Total biomasses of the stands of 14 years and 20 years located in gentle slopes and good soil conditions were 96.2t/ha and 145.0t/ha, and total net productions of those were 22.4t/ha/yr and 23.5t/ha/yr, respectively. Total biomasses of the stands of 6 years and 9 years located in steep slopes and poor soil conditions were 0.7t/ha and 14.0t/ha, and total net productions of those were 0.3t/ha/yr and 4.7t/ha/yr, respectively. As stand age increased, the ratios of stem wood and branches to total biomass and total net production increased, while the ratios of leaves to total biomass and total net production and the ratios of roots to total biomass decreased. Concentrations of N, P, K and Mg were greatest in the leaf and concentration of Ca was greatest in the stem bark. As stand age increased, N concentrations of the stem bark, branch, dead branch and root and K concentration of the branch decreased, while Ca concentration of the stem bark increased. Nutrient contents of the whole tree were great in order of N, K, Ca, Mg and P.

• Research in Plant Disease
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• v.19 no.1
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• pp.55-59
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• 2013

The purpose of this experiment was to investigate the development of new spot disease on the leaf and stem of rosemary (Rosmarinus officinalis) in commercial greenhouses at Jeonju and Namwon in Korea. Incidence of target spot on rosemary was higher at the end of the rainy season with high humidity. Those symptoms were black ring spots (3-5 mm in diameter) and withering on green leaves and stems. Conidiophores and conidia were formed on the infected tissue in moist chamber and conidia were shown as the cylindrical and oval types in chain, ranged from 55 to $275{\mu}m$ in length, and 7 to $14{\mu}m$ in width. Conidia with eight to ten pseudosepta were formed on the conidiapore. The optimum growth temperature of isolates was $30^{\circ}C$ on the PDA medium under the dark condition. In the pathogenesis test, the target spot and withering symptoms were appeared on the leaves and stems 3 days after inoculation showing similar symptoms compared to those of in nature. The same fungus was re-isolated from infected lesion, indicating that Corynespora cassiicola caused leaf target spot and twig blight on rosemary. The rDNA ITS nucleotide sequences of the pure cultured isolate from the diseased area on rosemary showed 100% similarity to the sequences of C. cassiicola available in the GenBank database (JQ595296, JQ595297, FJ852715 and AY238606). Therefore, we report that the target spot of leaves and stems in rosemary was caused by C. cassiicola.

• Journal of Plant Biotechnology
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• v.40 no.3
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• pp.147-155
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• 2013

In this experiment, we report for the first time mass propagation by in vitro mircrotuberization of Codonopsis lanceolata. We first examined the effect of cytokinins on multiple shoot induction. 2.0 $mg{\cdot}L^{-1}$ of kinetin not only gave the highest rate of shoot induction (19.1%) but also the elongation of shoot (17.1 mm). Secondly, we investigated the effect of sugars on in vitro microtuberization from nodal segments. The diameter of tuberous roots was enlarged in the half-strength MS medium supplemented with 145.9 mM sucrose. Histological analysis revealed that the number of parenchymatous cell containing starch grains increased in the tuberous roots. In addition, unlike in non-tuberous root, vascular bundles were scattered inner cortex layer. Thirdly, in order to preserve and stimulate the germination, microtubers were stored at $4^{\circ}C$ refrigerator during 9 months and then transplanted to the artificial soils (vermiculrite : peatmoss = 1:1 v/v), resulting that the rates of survival and germination were 75% and 70%, respectively. These results indicated that mass propagation of C. lanceolata was achieved by in vitro microtuber formation, suggesting that this protocol might be applied for not only the propagation of elite clones but also conservation of C. lanceolata germplasm.

• Ecology and Resilient Infrastructure
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• v.7 no.2
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• pp.90-96
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• 2020

Recently, a method that applies laser scanning (LS) that acquires vegetation information such as the vegetation habitat area and the size of vegetation in a point cloud format has been proposed. When LS is used to investigate the physical shape of vegetation, it has the advantage of more accurate and rapid information acquisition. However, to examine uncertainties that may arise during measurement or post-processing, the process of adjusting the data by the actual data is necessary. Therefore, in this study, the physical structure of stems, branches, and leaves of woody vegetation in an artificially formed river channel was manually investigated. The obtained results then compared with the information acquired using the three-dimensional terrestrial laser scanning (3D TLS) method, which repeatedly scanned the target vegetation in various directions to obtain relevant information with improved precision. The analysis demonstrated a negligible difference between the measurements for the diameters of vegetation and the length of stems; however, in the case of branch length measurement, a relatively more significant difference was observed. It is because the implementation of point cloud information limits the precise differentiation between branches and leaves in the canopy area.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.155-165
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• 1992

Experiments were conducted to determined the development of the vascular bundles in the peduncle of different tillers on its development in order to improve the vascular system and possibly increase grain yield. The development of the vascular bundle in the leaf, stem and panicle is an important aspect of assimilate translocation and differentiation of panicle characters. Two cultivars were used in this study: IR58, an indica type, and Unbong 7, a japonica type. The main culm(M) had more and bigger vascular bundles in the peduncle and those vascular bundle decreased with tiller order and tiller development. In the primary tillers, P1 had more large and small vascular bundles than P5 in both cultivars. IR58 developed more large vascular bundles compared to Unbong 7, but the small vascular bundle in unbong 7 was more than in IR58. The cross sectional area of phloem and xylem in large vascular bundle decreased with tiller order in both cultivar. Larger area of phloem and xylem in the early formed tillers more efficient transport of assimilates. The number of spikelets, the weight of panicle and grain yield per panicle were highest in the main culm followed by the order of their initiation or emergence. The number of primary and secondary branches to be positive associated with the number and area of vascular bundles. Furthermore, the number of vascular bundles in the peduncle was highly correlated with the peduncle thickness which in turn was correlated with the number of primary and secondary branches on the panicle. These results showed tillers that are initiated early and have relatively ation usually have more vascular bundles, larger peduncle, more spikelets spike let filling and ultimately higher yield.