Occurrence of Target Spot on Rosemary Caused by Corynespora cassiicola in Korea

Corynespora cassiicola에 의한 로즈마리 점무늬병

  • Lee, Wang-Hyu (Department of Agricultural Biology, Chonbuk National University(Plant Medical Research Center: Industry, Academy, Research, Cooperation Company for Herb, Jeollabuk-do)) ;
  • Han, Sang-Jun (Department of Agricultural Biology, Chonbuk National University(Plant Medical Research Center: Industry, Academy, Research, Cooperation Company for Herb, Jeollabuk-do)) ;
  • Choi, In-Young (Jeollabuk-do Agricultural Research and Extension Services)
  • 이왕휴 (전북대학교 농생물학과(전북대학교 식물의학연구센터, 전북허브산학협력단)) ;
  • 한상준 (전북대학교 농생물학과(전북대학교 식물의학연구센터, 전북허브산학협력단)) ;
  • 최인영 (전라북도농업기술원)
  • Received : 2012.10.04
  • Accepted : 2013.02.02
  • Published : 2013.03.31


The purpose of this experiment was to investigate the development of new spot disease on the leaf and stem of rosemary (Rosmarinus officinalis) in commercial greenhouses at Jeonju and Namwon in Korea. Incidence of target spot on rosemary was higher at the end of the rainy season with high humidity. Those symptoms were black ring spots (3-5 mm in diameter) and withering on green leaves and stems. Conidiophores and conidia were formed on the infected tissue in moist chamber and conidia were shown as the cylindrical and oval types in chain, ranged from 55 to $275{\mu}m$ in length, and 7 to $14{\mu}m$ in width. Conidia with eight to ten pseudosepta were formed on the conidiapore. The optimum growth temperature of isolates was $30^{\circ}C$ on the PDA medium under the dark condition. In the pathogenesis test, the target spot and withering symptoms were appeared on the leaves and stems 3 days after inoculation showing similar symptoms compared to those of in nature. The same fungus was re-isolated from infected lesion, indicating that Corynespora cassiicola caused leaf target spot and twig blight on rosemary. The rDNA ITS nucleotide sequences of the pure cultured isolate from the diseased area on rosemary showed 100% similarity to the sequences of C. cassiicola available in the GenBank database (JQ595296, JQ595297, FJ852715 and AY238606). Therefore, we report that the target spot of leaves and stems in rosemary was caused by C. cassiicola.

이 연구는 국내에 보고되지 않은 로즈마리 점무늬병이 남원과 전주의 재배 온실에서 발생하여 구명하고자 실험하였다. 이 병은 주로 고온 다습한 시기인 장마끝 무렵에 발생이 많았다. 로즈마리의 잎과 줄기에 검은점무늬(직경 3-5 mm) 및 시들음 증상이 나타났다. 병든 조직을 습실 처리하면 분생자경과 분생포자가 형성되었다. 로즈마리 점무늬병균의 분생포자는 체인상의 가늘고 긴 원통형으로, 길이는 $55-275{\times}7-14{\mu}m$이었다. 분생포자는 분생자경 위에 형성되었고, 분생포자의 위격벽의 수는 8-10개이었다. 또한 생장적온은 감자한천 배지와 암흑조건에서 $30^{\circ}C$이었다. 포트 식물에 병원성 검정결과 분리한 병원균은 접종 3일 후에 잎과 줄기에서 자연 병징과 같은 점무늬 및 시들음 증상이 재현되었고, 병든 부위에서 동일한 균이 재 분리되었다. 따라서 병원성이 있음이 확인되었다. rDNA ITS 영역의 염기서열 분석결과 로즈마리에 점무늬병을 일으키는 병원균은 Corynespora cassiicola로 GenBank accession number JQ595296, JQ595297, FJ852715, AY238606와 염기서열이 100% 일치하였고, C. cassiicola와 같은 계통군에 속하였다. 따라서, 로즈마리 잎 및 가지 점무늬병균은 C. cassiicola로 보고하고자 한다.



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