• Title/Summary/Keyword: 준비성

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Effects of Cooling Rate and Equilibration Time on the Survival and Development of Frozen-thawed Bovine Immature Oocytes (동결속도와 평형시간이 소 미성숙 난자의 동결 융해후 생존율에 미치는 영향)

  • 양병철;양보석;성환후;임기순;최선호;장원경;진동일;임경순
    • Korean Journal of Animal Reproduction
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    • v.25 no.1
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    • pp.19-28
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    • 2001
  • The present study was undertaken to investigate the effects of cooling rate and equilibration time on the survival, in vitro maturation and development to embryos of frozen-thawed bovine immature oocytes(Germinal Vesicle Stage). The cryoprotectants are used 10% ethylene glycol(EG) as permeating cryoprotectant and 0.05M soc.ose(S) or trehalose(T) as low molecular weight nonpermeating cryoprotectants and 5% ficoll(F) or polyvinylpyrrolidone(PVP) as high molecular weight nonpermeating cryoprotectants. Four freezing solution were uysed in this experiment(EFT: 10% EG + 5% F + 0.05M T, EFS: 10% EG + 5% F + 0.05M S, EPT: 10% EG + 5% P + 0.05M T, EPS: 10% EG + 5% P + 0.05M S). The best equilibration time and freezing solution was 15 min in EPT(83% survival rate of frozen-thawed bovine immature oocytes). When frozen-thawed bovine oocytes were cultured following IVM and IVF, there was no significant difference in cleavage and development rates among the EFT, EFS, EPT and EPS solutions. When 9 blastocysts derived from frozen bovine oocytes were transferred to 6 recipients, two recipients were pregnant. And one was aborted at 45 days of pregnancy and the other had a stillbirth.

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A Study on Animation Character Face Design System Based on Physiognomic Judgment of Character Study in the Cosmic Dual Forces and the Five Elements Thoughts (음양오행(陰陽五行)사상의 관상학에 기반한 애니메이션 캐릭터 얼굴 설계 시스템 연구)

  • Hong, Soo-Hyeon;Kim, Jae-Ho
    • Journal of Korea Multimedia Society
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    • v.9 no.7
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    • pp.872-893
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    • 2006
  • In this study, I classify the elements of physiognomic judgment of character with regard to form and meaning from a visual perspective based on physiognomic judgment of character study in 'the cosmic dual forces and the Five Elements theory'. Individual characters for each type are designed using graphic data. Based on that, design system of individual characters for each personality type is investigated using Neural Network system. Faces with O-Haeng (Five Elements) shapes are shown to constitute the system with ${\pm}0.3%$ degree of error tolerance for the non-loaming input data. For the shapes of Chinese characters 'tree, fire, soil, gold and water', their MSE(Mean Square Error) are 0.3, 0.3, 0.2, 0.5, 0.2. It seems to be the best regarding the scoring system which ranges from 0 to 5. Therefore, this system might be regarded to produce the most accurate facial shape of character automatically when we input character's personality we desire to make.

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Biocompatibility and Histopathologic Change of the Acellular Xenogenic Pulmonary Valved Conduit Grafted in the Right Ventricular Outflow Tract (우심실 유출로에 이식한 무세포화 이종 폐동맥 판막도관의 생체 적합성 및 조직병리학적 변화양상에 대한 연구)

  • 허재학;김용진;박현정;김원곤
    • Journal of Chest Surgery
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    • v.37 no.6
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    • pp.482-491
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    • 2004
  • Background: The xenogenic or allogenic valves after in Vitro repopulation with autologous cells or in vivo repo-pulation after acellularization treatment to remove the antigenicity could used as an alternative to synthetic polymer scaffold. In the present study, we evaluated the process of repopulation by recipient cell to the acellu-larized xenograft treated with NaCl-SDS solution and grafted in the right ventricular outflow tract. Material and Method: Porcine pulmonary valved conduit were treated with. NaCl-SDS solution to make the grafts acellularized and implanted in the right ventricular outflow tract of the goats under cardiopulmonary bypass. After evaluating the functions of pulmonary valves by echocardiography, goats were sacrificed at 1 week, 1 month, 3 months, 6 months, and 12 months after implantation, respectively. After retrieving the implanted valved conduits, histopathologic examination with Hematoxylin-Eosin, Masson' trichrome staining and immunohistochemical staining was performed. Result: Among the six goats, which had been implanted with acellularized pulmonary valved conduits, five survived the expected time period. Echocardiographic examinations for pulmonary valves revealed good function except mild regurgitation and stenosis. Microscopic analysis of the leaflets showed progressive cellular in-growth, composed of fibroblasts, myofibroblasts, and endothelial cells, into the acellularized leaflets over time. Severe inflammatory respon-se was detected in early phase, though it gradually decreased afterwards. The extracellular matrices were regenerated by repopulated cells on the recellularized portion of the acellularized leaflet. Conclusion: The acellularized xenogenic pulmonary valved conuits were repopulated with fibroblasts, myofibroblasts, and endothelial cells of the recipient and extracellullar matrices were regenerated by repopulted cells 12 months after the implantation. The functional integrity of pulmonary valves was well preserved. This study showed that the acellularized porcine xenogenic valved conduits could be used as an ideal valve prosthesis with long term durability.

Evaluation of the dietary quality and nutritional status of elderly people using the Nutrition Quotient for Elderly (NQ-E) in Seoul (노인 영양지수 (NQ-E)를 이용한 서울 일부지역 노인의 식생활 및 영양 상태 평가)

  • Ham, Sun-Wook;Kim, Kyung-Hee
    • Journal of Nutrition and Health
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    • v.53 no.1
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    • pp.68-82
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    • 2020
  • Purpose: This study evaluated the dietary quality and nutritional status of elderly people using the Nutrition Quotient for Elderly (NQ-E). Methods: The participants were 204 elderly people over 65 years of age (38 men and, 166 women) in Seoul. The dietary information was analyzed using a questionnaire of NQ-E, which consisted of 19 checklist items, and 24-recall test data. The NQ-E scores and its four factors, including 'balance', 'moderation', 'diversity', and 'dietary behavior' factors, were calculated according to general characteristics of the subjects. The subjects were divided into the 'monitoring needed group' (62 > NQ-E score) and the 'good group' (62 ≤ NQ-E score) according to their NQ-E score. Results: The mean NQ-E score of the total subjects was 61.9, which was within the mediumhigh grade. The scores of balance, moderation, and dietary behavior factors were within the medium-high grade, while the score of the diversity factor was within the medium-low grade. The NQ-E score was 54.8 in the monitoring needed group and 69.3 in the good group. For the score of the diversity factor, the elderly living alone had a significantly lower score than the score for the elderly living with a spouse. The female subjects showed significantly higher scores of moderation and dietary behavior factors than did the male subjects. The daily intakes of vitamin A, thiamin, riboflavin, vitamin C and calcium in the monitoring needed group were significantly lower than those in the good group. The nutrient adequacy ratios (NAR) of vitamin A, thiamin, riboflavin, vitamin C and calcium in the monitoring needed group were significantly lower compared to the scores of the good group. The indexes of nutritional quality (INQ) of vitamin A, thiamin, riboflavin, vitamin C, calcium and potassium were less than 1 for all the subjects. The monitoring needed group had a significantly lower consumption of total foods, vegetables and mushrooms than the good group. As a result, the nutritional status of the monitoring needed group was significantly lower than that of the good group. Conclusion: The results of this study show that NQ-E would be a useful tool for assessing the dietary quality of the elderly. In conclusion, a focused-nutrition education program and a useful guideline are needed for promoting the health and nutritional status in elderly people.

Analysis of Modality and Procedures for CCS as CDM Project and Its Countmeasures (CCS 기술의 CDM 사업화 수용에 대한 방식과 절차 분석 및 대응방안 고찰)

  • Noh, Hyon-Jeong;Huh, Cheol;Kang, Seong-Gil
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.15 no.3
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    • pp.263-272
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    • 2012
  • Carbon dioxide, emitted by human activities since the industrial revolution, is regarded as a major contributor of global warming. There are many efforts to mitigate climate change, and carbon dioxide capture and geological storage (CCS) is recognized as one of key technologies because it can reduce carbon dioxide emissions from large point sources such as a power station or other industrial installation. The inclusion of CCS as clean development mechanism (CDM) project activities has been considered at UNFCCC as financial incentive mechanisms for those developing countries that may wish to deploy the CCS. Although the Conference of the Parties serving as the Meeting of the Parties to the UNFCCC's Kyoto Protocol (CMP), at Cancun in December 2010, decided that CCS is eligible as CDM project activities, the issues identified in decision 2/CMP.5 should be addressed and resolved in a satisfactory manner. Major issues regarding modalities and procedure are 1) Site selection, 2) Monitoring, 3) Modeling, 4) Boundaries, 5) Seepage Measuring and Accounting, 6) Trans-Boundary Effects, 7) Accounting of Associated Project Emissions (Leakage), 8) Risk and Safety Assessment, and 9) Liability Under the CDM Scheme. The CMP, by its decision 7/CMP.6, invited Parties to submit their views to the secretariat of Subsidiary Body for Scientific and Technological Advice (SBSTA), SBSTA prepared a draft modalities and procedure by exchanging views of Parties through workshop held in Abu Dhabi, UAE (September 2011). The 7th CMP (Durban, December 2011) finally adopted the modalities and procedures for CCS as CDM project activities (CMP[2011], Decision-/CMP.7). The inclusion of CCS as CDM project activities means that CCS is officially accredited as one of $CO_2$ reducing technologies in global carbon market. Consequently, it will affect relevant technologies and industry as well as law and policy in Korea and aboard countries. This paper presents a progress made on discussion and challenges regarding the issue, and aims to suggest some considerations to policy makers in Korea in order to demonstrate and deploy the CCS project in the near future. According to the adopted modalities and procedures for CCS as CDM project activities, it is possible to implement relevant CCS projects in Non-Annex I countries, including Korea, as long as legal and regulatory frameworks are established. Though Korea enacted 'Framework Act on Low Carbon, Green Growth', the details are too inadequate to content the requirements of modalities and procedures for CCS as CDM project. Therefore, it is required not only to amend the existing laws related with capture, transport, and storage of $CO_2$ for paving the way of an prompt deployment of CCS CDM activities in Korea as a short-term approach, but also to establish the united framework as a long-term approach.

A Study on Stability of Inorganic Binder for Application as Conservation Material for Stone Monuments (석조문화재 보수물질로의 적용을 위한 무기질 바인더 안정성 연구)

  • Kim, Dae Sik;Do, Jin Young;Cho, Hyen Goo
    • Journal of the Mineralogical Society of Korea
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    • v.27 no.4
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    • pp.251-262
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    • 2014
  • Inorganic binders were studied in order to apply a conservation material for stone monument. A pure inorganic binder and 3 species of inorganic binder which contain additives on the basis of a pure inorganic binder were selected as test samples. Through the application of inorganic binders on Geochang granite investigate their influences on stone. pH 4.0 and 5.6 acid solution, respectively were manufactured on the basis of the acidity of domestic rainfall. Alkaline water with pH 8.0 and deionized water with pH 6.85 were prepared as control group. Changes in weights of inorganic binders were not definite according to the acidity of water while weight losses in inorganic binder type were greater after reaction with the water. The compressive strengths of pure inorganic binder was largest before the test but its decrease rate were larger after reaction with the water. Water absorption rate of inorganic binders are 6.72 to $12.44kg/m^2{\cdot}t^{1/2}$ after reaction with the water. Such high absorption was considered that it forced water to move deep into inorganic binder and made the components of inorganic binder dissolve. Acidities of the water of pH 4.0, 5.6, 6.85 and 8.0, respectively were changed to pH 9.0-10.0 after reaction with the inorganic binders. Ion concentrations in the water changed after reaction with the inorganic binders and $Mg^{2+}$, and $K^+$ significantly increased, dissolved from the binder. The high concentration of ions detected showed that the binder reacted with water and formed white salts with high solubility such as $MgSO_4{\cdot}nH_2O$, $KNO_3$. Ion concentrations significantly decreased from the binder after treatment with consolidant and water repellent.

Color stability of three dimensional-printed denture teeth exposed to various colorants (다양한 색소에 대한 3D 프린팅 인공치의 색 안정성)

  • Koh, Eun-Sol;Cha, Hyun-Suk;Kim, Tae-Hyung;Ahn, Jin-Soo;Lee, Joo-Hee
    • The Journal of Korean Academy of Prosthodontics
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    • v.58 no.1
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    • pp.1-6
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    • 2020
  • Purpose: This study evaluated color stability of Dentca 3D-printed denture teeth, in comparison to color stabilities of four conventional types of denture teeth, upon being immersed in various colorants. Materials and methods: Four types of conventional prefabricated denture teeth (Surpass, GC, Artic 6, Heraeus Kulzer, Premium 6, Heraeus Kulzer, Preference, Candulor), 3D-printed denture teeth (Dentca); and Z250 (Filtek Z250, 3M ESPE) were prepared for testing. The samples were immersed in erythrosine 3%, coffee, cola, and distilled water (DW) at 37℃. Color change (ΔE) was measured by spectrophotometer before immersion and at 7, 14, and 21 days after immersion. One-way analysis of variance was performed along with Tukey's honestly significant difference multiple comparisons test (P<.05). Results: No great difference was observed between the color change of Dentca denture teeth and that of conventional denture teeth in most cases (P>.05). The color change of Dentca denture teeth immersed in erythrosine 3% was greater than that of Surpass (ΔE = 0.67 ± 0.25) after 1 week; Artic 6 (ΔE = 1.44 ± 0.38) and Premium 6 (ΔE = 1.69 ± 0.35) after 2 weeks; and Surpass (ΔE = 1.79 ± 0.49), Artic 6 (ΔE = 2.07 ± 0.21), Premium 6 (ΔE = 2.03 ± 0.75), and Preference (ΔE = 2.01 ± 0.75) after 3 weeks (P<.05). Conclusion: A color change was observed in Dentca denture teeth when immersed in some colorants; however, the maximum value of ΔE for Dentca denture teeth was within the clinically acceptable range.

Stand Yield Table and Commercial Timber Volume of Eucalyptus Pellita and Acacia Mangium Plantations in Indonesia (인도네시아 유칼립투스 및 아카시아 조림지의 임분수확표 및 이용가능 목재생산량 추정)

  • Son, Yeong-Mo;Kim, Hoon;Lee, Ho-Young;Kim, Cheol-Min;Kim, Cheol-Sang;Kim, Jae-Weon;Joo, Rin-Won;Lee, Kyeong-Hak
    • Journal of Korean Society of Forest Science
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    • v.99 no.1
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    • pp.9-15
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    • 2010
  • This study was conducted to develop a stand growth model and a stand yield table for Eucalyptus pellita and Acacia mangium plantations in Kalimantan, Indonesia. To develop a stand growth model, Weibull robability density function, a diameter class model, was applied in this study. In the development of stand growth model by site index and stand age, a hierarchy is generally required - estimation, recovery and prediction of the diameter class model. A number of grow equations were also involved in each process to estimate diameter, height, basal area, minimum or maximum diameter. To examine whether the grow equations are adequate for Eucalyptus pellita or Acacia mangium plantations, a fitness index was analyzed for each equation. The results showed that fitness indices were ranged from 65 to 89% for Eucalyptus pellita plantations and from 72 to 95% for Acacia mangium plantations. As being highly adequate for the plantations, a stand yield table was developed based on the resulted growth model, and applied to estimate the stand growth with midium site index for 10-year period. The highest annual stand growth of Eucalyptus pellita plantations was estimated to be 21.25 $m^3$/ha, while that of Acacia mangium plantations was 27.5 $m^3$/ha. In terms of annual stand growth, Acacia mangium plantations appeared to be more beneficial than Eucalyptus pellita plantations. Also, to estimate commercial timber volume available from the plantations, an assumption that a log would be cut by 2.7 m in length and the rest of the log would be cut by 1.5m was involved. The commercial timber volume available from Eucalyptus pellita plantations was 68.0 $m^3$/ha, 33% from the total stand volume, 203.2 $m^3$/ha. Also 96.7 $m^3$/ha of commercial timbers were available from Acacia mangium plantations, which was 42% from the 232.9 $m^3$/ha in total. Presenting a good information about the stand growth in Eucalyptus pellita and Acacia mangium plantations, this study might be useful for whom proceeds or considers an abroad plantation for merchantable timber production or carbon credit in tropical regions.

Validation of the coach-athlete relationship scale of amateur golf players: Rasch rating scale model (아마추어 골프 선수를 위한 코치-선수 관계 척도의 타당화: Rasch 평정척도 모형 적용)

  • Kim, Sae Hyung;Choi, Jae Il;Lee, Jun Woo
    • Journal of the Korean Data and Information Science Society
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    • v.24 no.6
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    • pp.1319-1329
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    • 2013
  • The purpose of this research was to develop and validate the coach-athlete relationship scale suitable to amateur golf players by applying the Rasch rating scale model. As the coach-athlete relationship scale, the Korean form of scale developed by Kim and Park (2008), which was revised based on the evidence on the basis of inspection contents, was used to conduct a survey on 217 amateur golf athletes. And the unidimensionality, which is the basic assumption of the Rasch model, was verified using the WINSTEPS program, and the appropriateness of the item category was established through the step calibration. The goodness of fit of each question was tested through the goodness-of-fit index and the differential item functioning (DIF) was estimated according to the golf career. When the goodness-of-fit index estimated for each question was 1.30 or more it was judged unfit and the significance level in the analysis was all set as.05. The results of the analysis showed that the measures variance explained by the Rasch measurement model was more (33.7%) than 20%, so the unidimensionality assumptions of the 11 questions (..hospitable posture when my coach is teaching) were satisfied. The result of analyzing the item category (7 scale) with step calibration was found to be unfit, but in the result of reanalyzing by rescoring into a 5-point scale, it was found to be fit. Particularly, in the result of estimating the goodness-of-fit using the systematized item category (5 scale), Question 10 (...my best when my coach is teaching) and Question 11 were found to be unfit, and as a result of estimating the differential functioning item according to golf career, Question 11 was found to be unevenly differentiated according to golf career. So the 5-point scale of Question 9 after eliminating the two questions which were unfit and differentiated was validated to be the coach-athlete relationship scale suitable to amateur golf athletes.

Development of Junior high School Competition Program for Creative Problem Solving on the basis of Technological Hands-on Activities (중학교에서의 기술적 체험활동에 터한 창의적 문제해결 경진대회 프로그램 개발)

  • Kim, Darani;Yi, San-bong
    • 대한공업교육학회지
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    • v.36 no.1
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    • pp.149-166
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    • 2011
  • The purpose of this study was to develop a creative problem-solving competition program in order to provide an opportunity for junior high schoolers to solve problems on the basis of technological hands-on activities by deploying their creative problem-solving skills and to foster their cooperative and challenging spirits by tackling the given problems in group. Relevant literature and competition programs were analyzed to develop a junior high school competition program for creative problem solving on the basis of technological hands-on activities. This study was implemented in three stages: preparing, developing and applying. In the preparation stage, the content structure of technology and the fields of corresponding hands-on activities were investigated, and creativity theories were analyzed. Besides, domestic and foreign creativity-related competition programs were checked. In the development stage, an overview of the nature, object, question making and evaluation of a competition program for creative problem solving on the basis of technological hands-on activities was presented, and a program was developed, which consisted of three stages: planning, operating and evaluating. In the application stage, every student in a selected junior high school participated in the competition program. The findings of the study were as follows: First, the competition program developed in this study intended to offer a chance for every junior high schooler to solve problems on the basis of technological hands-on activities to display their creative problem-solving skills. Second, the program was designed to include planning, operating and evaluation stages, after the nature and object of the competition program and in which way question making and evaluation should be fulfilled were determined. Third, the competition program dealt with five areas according to the content structure of technology: manufacturing technology, construction technology, transportation technology, communication technology and biotechnology. And questions for each area and student worksheets were developed. Fourth, the program was utilized in a junior high school to give an example.