• Korean Journal of Poultry Science
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• v.29 no.3
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• pp.225-231
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• 2002

Total 240 of ISA Brown layers were employed in this experiment to study the effects of single or mixed feeding of Lactobacillus and yeast on the performance and intestinal microflora of laying hens. They were randomly allocated to six dietary treatments; None(Control), Pichia farinosa(PF), Lactobacillus crispatus avihen1 (LCH), Lactobacillus vaginalis avihen1(LVH), LCH＋PF, and LVH＋PF. Viable microflora were added to meet 3${\times}$10$\^$6/ cfu PF and 10$\^$7/ cfu Lacrobacillus per g of feed. There were four replicates per treatment, and 10 birds per replicates. Laying performance was recorded for 10 weeks, followed by a metabolism trial during which nutrient utilization, pattern of intestinal microflora and fecal NH$\sub$3/ emission were examined. Egg production and daily egg mass of birds fed either single or mixed microorganisms were significantly higher than those of the control(P<0.05). Egg weight and feed intake were not statistically different among all treatments. However, feed conversion ratio tended to improve by the supplementation of microbes. Digestibility of crude protein, ether extract and crude ash tended to improve in Lactobacillus treatments, however, there were not statistically different. With regards to the number of intestinal microbes, number of anaerobes were increased in microbes feeding group. Eggshell quality of PF layers was significantly poorer than those of the other treatments. No consistent trend was found in Haugh Unit among all treatments. Fecal NH$\sub$3/ gas emission was significantly lower in LVH, LVH＋PF and LCH＋PF than the other treatments(P<0.05). From the result of this experiment, it could be concluded that single or mixed feeding of Lactobacillus and yeast improves the laying performance and decreases the fecal ammonia gas emission. No synergic effect was found when both microbes were mixed and fed to the layers.

• Korean Journal of Poultry Science
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• v.35 no.4
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• pp.391-398
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• 2009

The objective of the present study was to compare of the effects of four molting methods on performance of laying hens. In total, 120 White Leghorn layers (62 weeks of age) were used. The four molting methods consisted of 10 days of feeding and the first 3 days of water withdrawal (FW1, 2) followed by ad libitum access to a layer diet 1 and 2 from day 11; 28 days of restricted feeding (NFW1); 28 days without withdrawing their feed (NFW2). Egg production of postmolt decreased until $3{\sim}4$ days at FW1 and FW2 treatments and until $16{\sim}18$ days at NFW1 and NFW2. 50% recovery of egg production were 7, 6, 7 and 5 week at FW1, FW2, NFW1, and NFW2 treatments, respectively. At day 14 of postmolt, the weight of livers, hearts, and oviducts decreased at molting treatments (P<0.05). Egg quality was improved on egg weight, eggshell thickness, eggshell break strength, and Haugh unit except egg yolk color (P<0.05).

• Korean Journal of Poultry Science
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• v.41 no.4
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• pp.241-247
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• 2014

The objective of this experimental was to investigate the effect of dietary Cu-sulfate and Cu-soy proteinate on productive performance and small intestinal microflora. A total 1,000 Hy-Line Brown laying hens (35 weeks old) were randomly allotted to 1 of 5 dietary treatments: Control, Cu-sulfate 50, 100 (50, 100 ppm Cu supplementation as Cu-sulfate) and Cu-SP 50, 100 (50, 100 ppm Cu supplementation as Cu-soy proteinate). Each treatment was replicated 4 times with fifty birds per replication, housed in 2 birds cages. Fifty birds units were arranged according to randomized block design. Feeding trial lasted 5 weeks under 16L : 8D lighting regimen. Hen day egg production was significantly (P<0.05) higher in Cu treated groups than control. Feed intake, broken and shell-less egg production was not significantly influenced by treatment. Eggshell color, eggyolk color, haugh unit, and eggshell thickness were not significantly influenced by treatment. However, eggshell strength was significantly (P<0.05) greater in Cu treated groups than control. Concentration of copper of liver was significantly (P<0.05) greater in Cu treated groups than control. Concentration of zinc and iron of liver were not influenced by treatments. Population of Cl. perfrigens and Lactobacilli in the small intestinal content were significantly (P<0.05) influenced by treatments. Population of Cl. perfrigens decreased and that of Lactobacilli increased in the Cu supplement groups. In conclusion, dietary Cu sulfate and Cu-soy proteinate similarly improves egg production, eggshell strength, and favors intestinal microbial population of laying hens.

• Korean Journal of Poultry Science
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• v.41 no.2
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• pp.143-149
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• 2014

This study was conducted to investigate the effects of dietary supplementation of the mixture of probiotics (MP) on growth performance, size of small intestine, cecal microflora and ammonia concentrations in broiler chicks. A total of 700, one-day-old male broiler chicks were randomly allotted to four treatments with seven replications having 25 birds per pen. The birds were fed one of the four experimental diets; containing no antibiotics nor MP (negative control, NC), containing antibiotics without MP(positive control, PC), negative control with MP 0.1% and negative control with MP 0.2% for 5 weeks. During overall experiment, birds in PC and MP treatments had higher final BW and daily BW gains than birds in NC treatments; however, the significance was not identified. The feed conversion ratio of the chicks fed the diet containing MP was significantly improved as compared to those fed the NC diets. The weights of jejunum were increased by the MP (p<0.05), but weights of duodenum, ileum and length of small intestine were similar among the groups. Birds in PC treatment showed lower populations of total microbes and lactic acid bacteria than other groups (p<0.05), cecal ammonia concentrations of the chicks fed the diet containing MP were significantly decreased as compared to those of NC and PC (p<0.01). In conclusion, MP added to the broiler diets improved the feed conversion rate and reduced cecal ammonia concentration.

• Clinical and Experimental Pediatrics
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• v.46 no.10
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• pp.966-971
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• 2003

Purpose : It is now well established that infection and inflammation play an important role in the pathogenesis of ischemic brain damage. The loss of neutrophils from systemic circulation is an associated finding in injury mediated by granulocyte. Periventricular leukomalacia(PVL) caused by ischemia is the principal form of brain injury in premature infants. This study was conducted to evaluate whether the low neutrophil count is associated with periventricular leukomalacia(PVL) in premature infants. Methods : Retrospective review of medical records was undertaken. Subjects were premature infants with a birth weight of less than 1,500 gm, admitted to the Neonatal Intensive Care Unit of Kyungpook University Hospital. A complete blood count of peripheral blood was done within the 1st hour of life. Neutropenia was defined as absolute neutrophil count < $1,500/mm^3$, PVL as increased periventricular echodensities followed by cyst formation on ultrasonography or corresponding signs on brain MRI. Results : Thirteen infants out of a total population of 37 revealed neutropenia. Respiratory distress syndrome and requirement for respiratory support were not different between infants with neutropenia( neutropenia group) and infants without neutropenia(control group). Intraventricular hemorrhage (IVH) and grade 3 and 4 IVH were more frequent in neutropenia group(P<0.05). There was no statistically significant increase of PVL in neutropenia group. The neutrophil count was $18,760.0{\pm}10,266.1/mm^3$, $7,272.0{\pm}7,435.0/mm^3$ infants with PVL and $11,131.7{\pm}3,386.5/mm^3$, $2,407.5{\pm}1,933.1/mm^3$ in infants without PVL, respectively. The frequency of mechanical ventilation and artificial surfactant therapy was higher in infants with PVL compared with infants without PVL, but statistical analysis was not performed due to small number of subjects. Conclusion : A low number of neutrophils in the systemic circulation was not associated with an increased risk of PVL in premature infants.

• Korean Journal of Poultry Science
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• v.35 no.2
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• pp.171-176
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• 2008

Animal welfare advocates, claim that the induced molting by fasting be avoided. This study was conducted to evaluate the effect of molting induced by various feeding methods on productivity and egg quality in laying hens. We used 400 flocks of 60-week-old leghorn laying hens in four treatments(five replicates of 20 hens each): fasting method (C), feeding single corn grain diet (T1), feeding single wheat bran diet (T2) and feeding single alfalfa meal diet (T3). As the result of the experiment, egg production and daily egg mass significantly decreased only in T1 compared with the control during the $1{\sim}4th$ week after the secondary egg laying (P<0.05). Although the amount of feed intake was significantly less in T1 group during the $1{\sim}4th$ week compared to the control, no significant difference was detected during the total period (P>0.05). In addition, no significant difference of feed conversion was observed between treatment groups. In terms of egg quality, the egg shell thickness was significantly improved in T1 group than the control group by feeding only corn at the 10th and 14th week after the secondary egg laying (P<0.05), but the significant decrease was observed at the corn (T1) and wheat bran (T2) fed treatment groups than the control group at the 26th week of the experiment (P<0.05). The eggshell strength and haugh unit did not show any difference by the molting methods. Egg yolk color was significantly decreased in T1 and T2 group than the control group at the 6th week (P<0.05). However, T1 and T2 group resulted to show significantly high egg yolk color at the 18th week of the experiment (P<0.05). As the result of the experiment, no large difference was observed in the productivity by the feeding molting method and by the fasting induced molting method. In addition, the single diet fed feeding induced molting method by using alfalfa revealed to show more satisfactory trend than the corn or wheat bran single diet fed feeding induced molting methods even if no statistically significant difference was found in terms of egg productivity.

• Korean Journal of Poultry Science
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• v.43 no.1
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• pp.15-20
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• 2016

This research was carried out to determine the effect of Cudrania tricuspidata fruit (CTF) on the performance of laying hens, egg quality, yolk fatty acid composition, Immunoglobulin G (IgG) and blood composition. A total 288 Brown Nick laying hens of 48 weeks old replaced into 4 groups with 4 replicates of 18 birds in each. Treatments were added with 0, 0.25, 0.5, 1.0% CTF containing dried seed and pulp. Metabolizable energy (ME) and crude protein (CP) were 2,800 kcal/kg and 16.0% in basal diets, respectively. Egg production and daily eggmass were significantly increased in 1.0% CTF addition treatment compared to those of other treatments (P<0.05). Egg weight and feed intake were not different among treatments. Feed conversion ratio showed the lower result in 1.0% CTF treatment than other treatments (P<0.05). Eggshell color was not influenced by the addition of CTF. Albumin height, Haugh unit and eggshell breaking strength was not statistically different, but seemed to be higher as dietary CTF addition increased. Yolk color was increased as dietary CTF increased and was the highest in 1.0% CTF group (P<0.05). Fatty acids were not shown the consistency in yolk. Blood cholesterol and triglyceride (TG) of birds fed CTF were significantly low compared to those of control. IgG was also statistically higher in CTF treatments than control (P<0.05). As the results of this experiment, the addition of 1% CTF would be the most effective on performance and egg quality, whereas the 0.25% CTF addition in basal diets seemed to be optimum to improve the blood cholesterol, TG,high density lipoprotein cholesterol (HDL-CHOL), IgG in laying hens.

• Korean Journal of Poultry Science
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• v.34 no.3
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• pp.207-215
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• 2007

This work was carried out to assess the effect of microbial phytase (MP) on the performance, retention and excretion of phosphorus, and nutrients availability in broiler chicks. Two hundred and sixteen one day old Ross strains broiler chicks were used to investigate on performance for 5 weeks (earlier $(0{\sim}2wk)$, finisher $(3{\sim}5wk)$) and were divided 3 treatments, three cages per treatment and 12 birds per cage. Treatments are P0 (basal diet), P1 (microbial phytase 300 FTU/kg), and P2 (microbial phytase 600 FTU/kg). After 5 weeks, three diets containing 0, 300, 600 FTU/kg of microbial phytase were fed to 5-wk-old broilers to assess the digestibility. Twenty seven broilers were divided into 3 treatments (9 birds per treatment) to investigate on the absorption and retention of phosphorus and nutrient digestibility. Body weight gain and feed efficiency were highly significant at P1 and P2 treatments compared to those of P0 (P<0.05), however, feed intake was no significant difference among all treatments (P>0.05). The excretion of phosphorus decreased in P1 and P2 compared to P0 (P<0.05). The digestibility of nutrients and amino acid was high in P1 and P2 treatments compared to P0 (P<0.05). Ileal digestibility was lower about $1{\sim}2%$ than feces digestibility (P>0.05). Finally, microbial phytase influenced on the performance, the retention and excretion of phosphorus and ileal and feces digestibility in broilers.

• Korean Journal of Poultry Science
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• v.41 no.4
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• pp.323-329
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• 2014

The objective of this experiment was to investigate the effect of dietary supplementation of copper-soy proteinate (Cu-SP) and herbal mixture (HBM) on growth performance, blood parameter, and immune response in laying hens. A total 800 Hy-Line Brown laying hens (60 weeks old) were randomly allotted to 1 of 4 dietary treatments : (1) Control : control diet, (2) Cu-SP : control diet + 100 mg/kg Cu-soy proteinate, (3) HBM : control diet + 0.15% herbal mixture, and (4) Cu-SP + HBM : control diet + 100 mg/kg Cu-soy proteinate + 0.15% herbal mixture. Each treatment was replicated 5 times with forty birds units were arranged according to randomized block design. Feeding trial lasted 5 weeks under 16L : 8D lighting regimen. The diet and water were available ad libitum. Result indicated that during feeding trial of the experiment, hen-day egg production was significantly (P<0.05) higher in Cu-SP and HBM treated groups than control. However, feed intake, feed conversion ratio, egg weight, broken and shell less egg production were not significantly influenced by treatments. Eggshell strength was significantly (P<0.05) higher in Cu-SP than control. Eggshell thickness, eggshell color, egg yolk color, Haugh unit were not significantly influenced by treatments. The level of WBC and stress index (heterophil : lymphocyte) were higher in supplemented groups than the control. The concentration of plasma IgG was higher in supplemented groups than the control. The result of this experiment showed that dietary copper-soy proteinate or herbal mixture tended to improve egg production and affect positively on immune response of laying hens.

• Korean Journal of Poultry Science
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• v.35 no.2
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• pp.131-136
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• 2008

This study was conducted to investigate the effects of dietary conjugated linoleic acid (CLA) and oil containing unsaturated fatty acid supplementation on egg production and quality in laying hens. Two hundred-eighty eight, 36 week old ISA brown commercial layer, were employed in a 5 week feeding trial. Dietary treatments are 1) BO (basal diet + 1% soybean oil + 1% oat), 2) BS (basal diet + 1% safflower oil + 1% oat), 3) BF (basal diet + 1% free fatty acid + 1% oat), 4) CD (basal diet + 1% CLA containing diglyceride + 1% oat), 5) CT (basal diet + 1% CLA containing triglyceride + 1% oat) and 6) CP (basal diet + 1% CLA by-product + 1% soybean oil). For overall period, hen-day egg production was not significant among treatments (P>0.05). Egg shell breaking strength at 3 weeks in CP treatment was significantly lower than BS, BF, CD and CT treatments (P<0.05) and that at 4 weeks in BO and CP treatments was significantly lower than others (P<0.05). Egg shell thickness at 3 and 4 weeks in CP treatment was significantly lower than BS, BF, CD and CT treatments (P<0.05). Egg weight at 3 and 4 weeks in CP treatment was significantly lower than others (P<0.05). Yolk height at 4 weeks in BF and CT treatments was significantly highest compared to BO, CD and CP treatments (P<0.05) and among BO, CD and CP treatments, that in BO and CD treatments was significantly higher than CP treatment (P<0.05). At 4 weeks, yolk color in CP treatment was significantly higher than BO and BS treatments (P<0.05). Haugh unit at 3 and 4 weeks in BO and CP treatments was significantly lower than others (P<0.05). In conclusion, supplementing CLA and oil containing unsaturated fatty acid for laying hens improved egg shell breaking strength, egg shell thickness, egg weight, yolk height, yolk color and haugh unit.