• Journal of Food Hygiene and Safety
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• v.35 no.6
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• pp.574-580
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• 2020

In this study we sought to determine the food fraud by discriminating species of commercial seafood product such as Larimichthys polyactis, Larimichthys crocea, Pennahia argentatus, and Miichthys miiuy, which are difficult to morphologically discriminate. After amplifying the mitochondrial cytochrome c oxidase subunit I gene of the reference fish, the DNA sequences of the amplified PCR products were analyzed. As a result, a 655 bp sequence for species identification was selected for use as DNA barcodes. To confirm the DNA data and primer set, the DNA barcode sequence of each fish was compared to that in that in the NCBI. All of the DNA barcode data were matched with the gene sequence of each fish in the NCBI. A total of 32 processed seafood products (8 L. polyactis, 12 L. crocea, 3 Pennahia argentatus, and 9 Miichthys miiuy) were investigated. Homology of 97% or more in DNA sequences was judged as the same species. As a result of the monitoring, there were no discovered cases of forgery or alteration. However, the use of a raw material name having no matching standard name in the Korea Food Code may cause consumer confusion. Therefore, it is suggested that the standard name or scientific name be co-labeled with the raw material name on seafood products to prevent consumer confusion.

• Korean Journal of Ichthyology
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• v.29 no.1
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• pp.1-12
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• 2017

A natural hybrid of interspecific between the Coreoleuciscus splendidus and C. aeruginos (Cypriniformes: Cyprinidae) was captured in the Hwang-ji Stream, a tributary of the Nakdong River basin in Korea. An interspecific hybrid between C. splendidus and C. aeruginos was genetically identified based on morphological characteristics and the sequence analysis of nuclear recombination activating gene 1 (RAG1) gene (1,334 bp) and mitochondrial cytochrome c oxidase subunit 1 (CO1) gene (1,551 bp). As a result of morphological variations, the natural hybrid appeared to have an intermediate character between two parental species (C. splendidus and C. aeruginos) in three variations of black array (s) on dorsal, caudal and anal fin rays. Phylogenetic analysis inferred from RAG1 and CO1 sequence data revealed that Coreoleuciscus populations from Hwang-ji stream consist of two pure Coreoleuciscus species and a hybrid individual group. The individuals were clearly identified the cross and reciprocal hybrid by CO1 gene analysis. In RAG1 gene, 13 nucleotide variation loci were detected and the hybrid individuals displayed the double peaks of sequence chromatograms at the 9 diagnostic positions. In this study, molecular data and morphological variations were clearly demonstrated that hybridization did occur between C. splendidus and C. aeruginos. However, F2 hybrid generation and reproductive capacity of F1 hybrid individuals were not demonstrated.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.375-382
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• 2015

We classified the forest type and figured out the ecological characteristics for each of the types in order to provide the basic informations for being induced ecologically efficient forest practice plan by vegetation units in the natural forest of Songnisan. We established the 250 sample points and collected the vegetation data of vertical distribution for each sample. A variety of multivariate statistical methods were applied to classify the forest types. The species diversity index were analyzed to estimate the stability and maturity for forest vegetation in each the type. The types were divided from two to ten clusters by cluster analysis. The appropriate number of clusters was estimated five clusters by indicator species analysis. It was verified through the multiple discriminant analysis that the estimated number of clusters had been suitable. Based on the species composition for each the type, this study site was classified into five forest types: 1) Quercus serrata and 2) mixed mesophytic forest in the valley area, 3) Q. mongolica forest in the main ridge, 4) Pinus densiflora forest in the sub-ridge extending from the main, and 5) Q. variabilis-P. densiflora forest between the sub-ridge and valley. The species diversity index of the pine forest that had been a simple species composition was the lowest while that of the mixed mesophytic forest of which the composition had been diverse was the highest. As the forest vegetation was more varied, the index showed a tendency to increase.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.3 no.2
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• pp.71-79
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• 1998

Fish larvae and environmental variables were collected in the sea around Cheju Island of Korea in November, 1986. Fish larvae were found at the 43 of 44 stations. Abundance of fish larvae rangεd from 3 to 259 indiv./$10^3\;m^3$ (average 50 indiv./$10^3\;m^3$) Dominant species were Engraulis japonicus, Champsodon snyderi, Gobiidae, Sebastiscus marmoratus, and Synagrops philippinensis, which comprised 56.6% of total fish larvae abundance. 43 stations were c1assified into two groups by c1uster analysis based on the abundance of fish taxa. Most stations of Group I were located in the middle reach of Tsushima Current and had higher number of species and abundance than the sites of Group II which cover the marginal areas of Tsushima Current, Korean Coastal Waters, and coastal water of China. The dominant taxa in the sites of the Group I were E. japonicus, Gobiidae, S. philippinensis, Aulops japonicus, Bregmaceros spp., Benthosema pterotum, Lampanctus spp., etc., all of which are typical warm-water species. Therefore, Tsushima Current seems to be the most critical factor regulating the structure of fish larvae community in the study area. The discriminant analysis using the environmenta1 variables (temperature, salinity, and dissolved oxygen) produced result with a difference only of 11.6% from that of c1uster analysis using the taxa composition and abundance data. Consequently, the structural type of fish larvae community can be used for predicting the changes of environmental conditions in the study area.

• Journal of Food Hygiene and Safety
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• v.37 no.1
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• pp.15-20
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• 2022

The purpose of this study was to compare the conventional culture method, enzyme immune method and the PCR method using species-specific primer in the analysis on the Salmonella spp. found in domestically distributed pet foods. For the study, Salmonella spp. were detected from 175 samples. From the conventional culture method and the PCR method, two samples (jerky and corn gluten) were determined as positive. Also, from the enzyme immune method, one sample (corn gluten) was test-positive. The study revealed that application of the PCR method with species-specific primer allows better distinguishment between the species of the strain collected from the samples than the conventional culture method and/or the enzyme immune method.

• Journal of Food Hygiene and Safety
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• v.39 no.3
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• pp.209-220
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• 2024

Cavier is graded as Beluga, Osetra, and Sevruga based on the species of sturgeon (Acipenser sinensis). In this study, we developed an analytical method for determining the grade of black caviar using DNA barcodes and KASP markers. To identify the sturgeon species, ten black caviar samples were collected, and a reference DNA barcode library was developed using five genes (namely, 16S ribosomal RNA, cytochrome b, cytochrome c oxidase subunit I, cytochrome c oxidase subunit II, and NADH dehydrogenase subunit 5 genes). To develop the KASP markers, we selected 11 markers that could distinguish between the five grades of black caviar. As a result, specific markers for each of the targeted caviars were clustered into FAM-positive sections. DNA barcoding and the KASP assay revealed that one Beluga, six Osetra, and three Sevruga were identified among the ten caviar samples. Moreover, we found that the sturgeon species were mislabeled in two products. Here, we aimed to develop a KASP assay based on SNP that allows rapid and easy identification of caviar grade. These methods are expected to contribute to preventing the distribution of illegal products.

• The Korean Journal of Applied Statistics
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• v.23 no.1
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• pp.13-28
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• 2010

Assuming a mixture distribution for credit evaluation studies, we discuss estimating threshold methods to minimize errors that default borrowers are predicted as non defaults or non defaults are regarded as defaults. A method by using statistical hypotheses tests, the most powerful test and generalized likelihood ratio test, for the probability density functions which are defined with the score random variable and the parameter space consisted of only two elements such as the default and non default states is proposed to estimate a threshold. And anther optimal thresholds to maximize classification accuracy measures of the accuracy and the true rate for ROC and CAP curves are estimated as equations related with these probability density functions. Three kinds of optimal thresholds in terms of the hypotheses testing, the accuracy and the true rate are obtained from normal random samples with various means and variances. The sums of the type I and type II errors corresponding to each optimal threshold are obtained and compared. Finally we discuss about their efficiency and derive conclusions.

• Economic and Environmental Geology
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• v.40 no.4
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• pp.375-387
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• 2007

The Iksan Mireuksaji stone pagoda, designated as national treasure No. 11 in Korea, has been in the process of disassembling for reconstruction as part of the cultural heritage conservation program. The pagoda is mainly consisting of granite, which is relatively resistant to weathering. However, it has lost its original rock color due to various contaminants deposited at the surface since it exposed to the atmospheric environment long time. In this research, we categorized the secondary inorganic contaminants into the genetic type, and also quantitatively examined occurrences and types of pollutants in the oxidation area of the pagoda surface in which the area is clearly distinguished by naked eyes. Geochemical behavior of soluble pollutants through reaction experiments are demonstrated, and effective methods of cleaning for the conservation and scientific applicability of the surface cleaner are also studied.

• Journal of the Korean Wood Science and Technology
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• v.34 no.6
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• pp.96-103
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• 2006

A simple method for identifying of ectomycorrhizal fungi was presented, using the polymerase chain reaction (PCR) to amplify the ITS (Internal transcribed spacer) regions of the nuclear ribosormal repeat. The sequences analyzed 6 species, Pisolithus tinctorius, Chroogomphus rutilus, Leucogyrophana pinastri, Suillus granulatus, Lactarius laeticolorus, and Suillus bovinus at hongreung forest, and analysed 10 species, Craterellus lutescens, Thelephoroid mycorrhizal, Lactarius quieticolor, Tricholoma matsutake, Lactarius chrysorrheus, Sarcodon aspratus, Russula versicolor, Suillus luteus, Tricholoma terreum, and Amanita vaginata at hongcheon forest. Finally, the amplification by PCR with ITS1-ITS4 primers offers good results over classical identification for ectomycorrhizal fungi species.

• Analytical Science and Technology
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• v.24 no.2
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• pp.85-93
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• 2011

To protect the steel structure in a high story buildings from fire, the sprayed fire-resistive materials are applied during the construction. Current standard methods to check the quality of sprayed fire-resistive materials are real fire test in lab, which take a long time (several weeks) and expensive. In this study, a simple analytical method to check the quality of sprayed fire-resistive materials is developed using Near Infrared Spectroscopy (NIR). Total 9 kinds of sprayed fire-resisted materials and 3 kinds of normal sprayed material sets were used for the analysis. Each set of materials was 50 to 100 samples. Samples are grinded and make a fine powder. The spectral data acquisition was carried out using FT-NIR spectrometer with a integrating sphere. NIR methods successfully identify the sprayed fire resistive materials by a principle component analysis (PCA) after a vector normalization (SNV) pretreatment.