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Identification of Tricholoma matsutake in a Pine Root by ITS Region Amplification and RAPD Analysis with Different Mycorrhiza  

Kim, Myungkil (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute)
Ryu, Sun-Hwa (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute)
Bak, Wonchull (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute)
Park, Hyun (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute)
Ka, Kang-Hyeon (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute)
Sohn, Hee-Kyung (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute)
Publication Information
Journal of the Korean Wood Science and Technology / v.34, no.6, 2006 , pp. 96-103 More about this Journal
Abstract
A simple method for identifying of ectomycorrhizal fungi was presented, using the polymerase chain reaction (PCR) to amplify the ITS (Internal transcribed spacer) regions of the nuclear ribosormal repeat. The sequences analyzed 6 species, Pisolithus tinctorius, Chroogomphus rutilus, Leucogyrophana pinastri, Suillus granulatus, Lactarius laeticolorus, and Suillus bovinus at hongreung forest, and analysed 10 species, Craterellus lutescens, Thelephoroid mycorrhizal, Lactarius quieticolor, Tricholoma matsutake, Lactarius chrysorrheus, Sarcodon aspratus, Russula versicolor, Suillus luteus, Tricholoma terreum, and Amanita vaginata at hongcheon forest. Finally, the amplification by PCR with ITS1-ITS4 primers offers good results over classical identification for ectomycorrhizal fungi species.
Keywords
ITS region; Tricholoma matsutake; Mycorrhiza; RAPD;
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