• Title/Summary/Keyword: 종자 수

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Callus Induction and Plant Regeneration from Mature Seeds of Timothy (티모시 성숙종자로부터 캘러스 유도 및 식물체 재분화)

  • Lee, Ki-Won;Kim, Ki-Yong;Choi, Gi-Jun;Lim, Young-Chul;Kim, Won-Ho;Jung, Min-Wong;Seo, Sung;Lee, Byung-Hyun;Lee, Sang-Hoon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.28 no.3
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    • pp.165-170
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    • 2008
  • Timothy (Phleum pratense L.) is an important grass species as forage. In order to optimize tissue culture conditions of timothy, the effects of plant growth regulators on callus induction and plant regeneration was investigated with mature seeds of colt cultivar. The optimal concentration of 2,4-D for the induction of primary callus from mature seeds was 3 mg/L. The highest embryogenic callus frequenc (25%) was observed when the mature seed were cultured on MS medium supplemented with 3 mg/L 2,4-D and 0.1 mg/L BA. The highest plant regeneration frequency was observed when type B callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of timothy by the production of transgenic plant.

A Standard Rose Cultivar 'Love Letter' with Thornless Stems and Red Colored Petals for Cut Flowers (무가시성 적색 절화장미 '러브레터' 육성)

  • Lee, Young Soon;Jung, Yun Kyung;Park, Mi Ok;Lim, Jae Wook
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.269-275
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    • 2014
  • A standard rose cultivar, 'Love Letter' was selected for cut flower from the progenies of a cross between 'Red Giant' and 'Ensemble' by the rose breeding team of the Gyeonggi-do Agricultural Research & Extension Services (GARES) in 2011. A standard rose cultivar 'Red Giant' with red colored (RHS Red Group 45C) petals and 1.8 prickles per stems of 10 cm was used as a mother plant. A standard rose cultivar 'Ensemble' with white and red purple colored (RHS White Group 155C + RHS Red Group N57B) petals and 2.4 prickles per stems of 10 cm was used as a pollen parent. 'Love Letter' was crossed in 2007 and seedlings were produced. After tests of specific characters from 2008 to 2011, this cultivar was finally selected and named. As a standard type with large sized flower, it has red colored (RHS Red Group 46A) petals with 9.3 cm flower diameter and 32.4 petals per flower. Vase life of this cultivar could be as long as 12 days. It takes 43 days from pruning to blooming and cut flower productivity was 152 stems/$m^2$ in a year. The stems of cut flower have no thorn and the length is about with 70.5 cm. When this cultivar grew below $15^{\circ}C$ and 1,000 lux, the petals color became dark red in winter season. 'Love Letter' was registered as a new cultivar on Korea Seed & Variety Service (KSVS) with No. 4482 on May 8, 2013.

Evaluation of Forage Production of Sorghum for High-Yielding Hybrid (우량 품종의 선발을 위한 사초용 수수의 생산성 평가)

  • Kim, J.D.;Kwon, C.H.;Kim, S.G.;Park, H.S.;Ko, H.J.;Kim, D.A.
    • Journal of Animal Science and Technology
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    • v.44 no.5
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    • pp.625-632
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    • 2002
  • Livestock farmers face several limitations when using the government recommended sorghum cultivars for forage because of the limited seed supply. Therefore, the objective of the this study is to evaluate, select, and recommend the best high-yielding hybrid as the government recommended cultivars. The agronomic characteristics and forage yield of three cultivars (cv. P947, cv. KF429 and cv. SS405) of forage sorghum were evaluated at two locations (Suwon and Sunghwan) for 2 years (1999${\sim}$2000). 'KF429' was susceptible to foliar disease, while 'SS405' had less lodging resistance among the sorghum cultivars tested. When compared with 'P947' (recommended cultivar), dry matter yield of 'KF429' and 'SS405' cultivars increased by 16% and 75%, respectively. The crude protein of 'P947'(9.4%) was higher than other cultivars. The percentage of ADF and NDF were the lowest in 'KF429' cultivar. The results of forage performance experiment indicate that 'KF429' and 'SS405' are recommended as the government recommended sorghum cultivars due to a high lodging tolerance, disease resistance, and high dry matter yield.

Influences of Seeding Dates and Pinching Height on Tender Shoot Productivity of Moloheiya (Corchorus olitorius L.) (모로헤이야 파종시기와 적심높이에 따른 신초의 생산성 비교)

  • Uhm, Mi Jeong;Kwon, Sung Whan;Kim, Hee June;Song, Young Ju
    • Journal of Bio-Environment Control
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    • v.24 no.3
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    • pp.196-201
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    • 2015
  • This study was conducted to seek optimum sowing time and pinching height for tender shoots production of moloheiya (Corchorus olitorius L.), which is a subtropical leaf vegetables, under an unheated greenhouse in the south of Korea. Seeds of moloheiya did not germinate at all at $10^{\circ}C$, but germinated well at $18{\sim}30^{\circ}C$ with 95.5~98.5% germination rate. Germination rate was 75.0% at $15^{\circ}C$, but germination speed was very low. The pods of moloheiya sown before April 28 was matured after late-August, but those sown after May 7 was not matured in that year. In tender shoot production and harvest frequency, treatment sown on April 14 was most plentiful among sowing time treatments, and showed marketable yield increase by 14~42% compared to other sowing times. Mean-while, pinching at 100cm high from soil surface was more effective than other heights for increment of branches and tender shoot. All above suggest that it was suitable to sow seed on mid-April and to pinch at 100cm high from soil surface for increment of tender shoots in the south of Korea.

Detection for the Resistance of Fusarium spp. Isolated from Rice Seeds to Prochloraz and Cross-resistance to Other Fungicides Inhibiting Sterol Biosynthesis (벼 종자에서 분리한 Fusarium속 균주들의 prochloraz에 대한 저항성 검정 및 교차 저항성 조사)

  • Shin, Myeong-Uk;Kang, Hyo-Jung;Lee, Yong-Hwan;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.12 no.3
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    • pp.277-282
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    • 2008
  • To assess the resistance to prochloraz, $EC_{50}$ values of Fusarium isolates obtained from rice seed were investigated through the agar dilution method. $EC_{50}$ value of 36 isolates of Fusarium spp. to prochloraz ranged from 0.020 to $1.78{\mu}g\;mL^{-1}$ with an average of $0.25{\mu}g\;mL^{-1}$. According to the species of Fusarium, the average $EC_{50}$ value was fluctuated; $0.091{\mu}g\;mL^{-1}$ for F. moniliformis, $0.11{\mu}g\;mL^{-1}$ for F. proliferatum and $0.31{\mu}g\;mL^{-1}$ for F. fujikuroi. The resistant baseline was decided at $0.5{\mu}g\;mL^{-1}$ to determine if the isolate was resistant to prochloraz or not. Based on the resistant baseline, the ratio of resistant isolates was 14%. There was no correlation between the resistance to prochloraz and the pathogenicity of Fusarium spp. on rice seedlings. The resistant isolates of F. fujikuroi did not show the cross-resistance to other sterol biosynthesis inhibiting fungicides, triflumizole, hexaconazole, difenoconazole and tebuconazole.

Effect of Bacillus subtilis C4 and B. cereus D8 on Plant Growth of Canola and Controlling Activity Against Soft Rot and Stem Rot (Bacillus subtilis C4와 B. cereus D8에 의한 유채의 생육증대 및 무름병과 균핵병 방제효과)

  • Lee, Jae-Eun;Lee, Seo-Hyeun;Park, Kyung-Soo;Park, Jin-Woo;Park, Kyung-Seok
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.275-282
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    • 2009
  • The effect of two plant growth-promoting rhizobacteria (PGPR) on plant growth and systemic protection against soft rot disease and stem rot disease of canola (Brassica napus), caused by Erwinia carotovora and Sclerotinia sclerotiorum was investigated in a laboratory and a greenhouse. Selected PGPR strains C4 and D8 were treated to canola seeds by soaking. Strains C4 and D8 significantly not only increased plant height and root length about 74% and 40.3% and also reduced disease severity of soft rot disease by 80% by C4 and D8 respectively, compared to the control. Especially strain C4 showed antifungal activity against 6 fungal pathogens, S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Colletotrichum acutatum. In greenhouse experiment, the seed treatment of both of them increased plant height, leaf width and leaf length of canola plant to 19.5% and 24.9%, 11.3% and 15.3%, and 14.1% and 20.7% by C4 and D8, respectively, and reduced disease severity of S. sclerotiorium. These results indicate that these two PGPR strains can decrease disease severity and increased plant growth under greenhouse condition. Therefore, these two bacteria have a potential in controlling Sclerotinia stem rot of canola. These strains have to investigate under field condition to determine their role of antibiosis, induced systemic resistance and plant growth promotion on canola.

Investigation of the Medicinal Plant Resources (약용식물(藥用植物)의 유전자원수집(遺傳資源蒐集)과 이용(利用)에 대(對)한 조사(調査))

  • Lee, Joon Tak
    • Current Research on Agriculture and Life Sciences
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    • v.8
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    • pp.71-82
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    • 1990
  • In order to collect the plant genetic resources in Korea, the medicinal plants were surveyed from 1987 to 1989 in Kyungbuk province and at the several areas in Kyuognam and Choongbuk province. The medicinal plants of 456 species in 118 families were investigated in these regions. Among them, 249 species were belonged to 19 families, Compositae, Liliaceae, Leguminosae, Poiygonaceae, Labiatae, Rosaceae, Umbelliferae, Gramineae, Solanaceae, Cucurbitaceae, Ranunculaceae, Cruciferae, Rutaceae, Araliaceae, Araceae, Moraceae, Scrophulariaceae, Caryopyhllaceae, and Convolvulaceae, and others were belonged to 99 families. They have been used mostly for urination, gastroenteric disoder, robustness, antiphlogistic, anodyne, cough medicine, fever remedy, hemostatic, and 9 species of them have been used for anticancer medicine. The medicinal plant resources which were planted in the fields, gardens, parks, roadsides, and glass house, were 93 species for ornamental, 50 for medicine, 27 for vegetable, 14 for food, 5 for vegetable fruit, 13 for fruit, 5 for oil, 6 for tea, 9 for shade, and 14 for other purposes. Most of investigated plants were perennial, and flowered from June to August. The root parts were most frequently used as medicine, and other parts were used in the order of leaf and stem, whole plants, flower, seed, and fruit. Among the 456 species, 191 species in 87 families were investigated to be important medicinal plants.

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Herbicidal Activity of Korean Native Plants (III) (살초활성물질 함유 국내 자생식물의 탐색 (III))

  • Kim, Song-Mun;Kim, Mi-Sung;Lee, Yu-Sun;Kim, Hee-Yeon;Choi, Hae-Jin;Heo, Su-Jeong;Kwon, Soon-Bae;Kim, Kyung-Hee;Han, Sang-Sub;Lim, Sang-Hyun
    • The Korean Journal of Pesticide Science
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    • v.9 no.2
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    • pp.173-180
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    • 2005
  • This study was conducted of Korean native plants to screen herbicidal activity which could be used for the development of new natural herbicides. Eighty-one plants were collected from Wan and Juju islands in Korea and their methanol extracts were obtained. Heibicidal activities of the methanol extracts were determined by seed bioassay using rapeseed (Brassica napus L.) seedlings. Among eighty-one species, eleven plants were highly herbicidal ($GR_{50}$ < 1,000 ${\mu}g\;g^{-1}$): Abies koreana WILS., Anthemis nobilis L., Callicarpa mollis SIEB. et ZUCC., Cirsium setidens, Euonymus maackii RUPR., Euphorbia jolkini BOISS., Kadsura japonica DUNAL, Paeonia aliflora Pall var. tricocarpa BUNGE, Philadelphus tenuifolius RUPR. et MAXIM., Sapium sebiferum, Vicia angustifolia var. segetilis K. KOCH. Fifty plants were shown moderate herbicidal activity (1,000 ${\mu}g\;g^{-1}$ < $GR_{50}$ < 2,000 ${\mu}g\;g^{-1}$), however, twenty plants were not shown any herbicidal activity.

Effects of Different Water Depths on Emergence of Barnyardgrass, Echinochloa crus-galli P. Beauv. (피의 발생(發生)에 미치는 담수심(湛水深)의 영향(影響))

  • Kang, Byeung-Hoa
    • Korean Journal of Weed Science
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    • v.6 no.1
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    • pp.7-12
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    • 1986
  • This experiment was conducted to investigate effects of different water depths on emergence, emerged soil depth and growth of E. crus-galli in Seoul, Korea. Plant number of emergence was decreased with deepened water depth, and dry weight of plant was especially negatively affected. However, a few E. crus-galli were emerged under 24 cm water depth. Soil depth of germination was increased as water depth decreased, and a few E. crus-galli were emerged below 10 cm soil depth under - 12 ㎝ water depth. But most E. crus-galli were emerged in ca. 2 cm soil depth under water depth over 0 cm.

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Effect of Several Cultivation on Growth of Lysimachia mauritiana Lam. as Potted Plant (몇 가지 재배조건이 분화용 갯까치수염(Lysimachia mauritiana Lam.)의 생육에 미치는 영향)

  • Jeong, Eun-ah;Jeon, Ki-beom;Lee, Jae-hyo;Choi, Hye-min;Moon, Sang-a;Yeon, Su Ho;Lee, Seong Yeon;Kwon, Soon-tae;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.58-58
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    • 2018
  • 본 연구는 관상식물인 갯까치수염의 분화용 재배기술을 개발하기 위하여 실시하였다. 종자를 이용하여 육묘된 유묘를 사용하여 분화용 포트의 크기, 상토 혼합조성, 기비처리, 유묘의 소질 등의 이식조건과 추비농도, 차광정도, 왜화제 및 적심처리 등의 재배, 관리조건에 대한 생육효과를 2017년 7월 31일부터 10월 9일까지 약 10주간 조사하였다. 갯까치수염의 분화용 재배를 위해서, 피트모스:펄라이트(3:1) 혼합 용토에 $300-200-200mg{\cdot}L^{-1}$(N-P-K)의 비료분을 첨가한 토양을 이색칼라 10호 화분에 충진하여 코트비료 10알을 기비로 시비하고, 200구 트레이에 셀당 2립씩 파종된 묘를 이식묘로 사용하는 것이 갯까치수염의 생육에 가장 효과적이었다. 재배 및 관리방법으로는 차광처리는 하지 않고 재배하는 것이 생육에 좋으며, $250mg{\cdot}L^{-1}$ hyponex를 추비하고 왜화제로 $500mg{\cdot}L^{-1}$ daminozide를 처리하는 것이 갯까치수염의 분화재배에 적합하였다. 또한, 재배기간이 10주 정도일 때는 적심을 하지 않는 것이 분화용 생산에 효과적일 것으로 판단되었다. 본 연구 결과는 다양한 활용성을 가진 야생 초화류의 이용성 및 부가가치 향상을 위한 대량생산 방법의 기초자료로 활용될 수 있을 것으로 생각된다.

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