농약과학회지 (The Korean Journal of Pesticide Science)

  • 제13권4호
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  • Pages.275-282
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  • 2009
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지

Bacillus subtilis C4와 B. cereus D8에 의한 유채의 생육증대 및 무름병과 균핵병 방제효과

Effect of Bacillus subtilis C4 and B. cereus D8 on Plant Growth of Canola and Controlling Activity Against Soft Rot and Stem Rot

  • 이재은 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이서현 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박경수 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박진우 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박경석 (농촌진흥청 국립농업과학원 농업미생물과)
  • Lee, Jae-Eun (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Lee, Seo-Hyeun (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Park, Kyung-Soo (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Park, Jin-Woo (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Park, Kyung-Seok (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA)
  • 발행 : 2009.12.31
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초록

선발된 PGPR 균주인 Bacillus subtilis C4와 Bacillus cereus D8 균주의 유채에 대한 생육촉진 및 무름병균인 Erwinia carotovora와 균핵병균인 Sclerotinia sclerotiorum에 대한 방제효과를 검정하기 위하여 실내검정과 온실검정을 실시하였다. 실내검정 결과, C4와 D8균주처리에 의하여 유채의 생육이 40.3%~74% 증가하였으며 무름병이 대조구에 비하여 80% 감소하였다. 실내검정에서 C4와 D8균주를 종자에 처리하였을 때 뿌리가 크게 신장되었다. 주요 식물병원균 Sclerotinia sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici, Colletotrichum acutatum에 대하여 항균활성시험을 수행한 결과 두 균주 중 C4균주는 모든 병원성 곰팡이에 대하여 항균활성을 나타내었다. 온실검정에서 C4와D8균주처리는 대조구에 비하여 유채의 초장, 엽폭 및 엽장을 19.5%~24.9%, 11.3%~15.3%, 14.1%~20.7% 각각 증가시켰으며 균핵병균인 Sclerotinia sclerotiorum에 대한 억제효과가 우수하였다. 이와 같은 결과를 볼 때 C4, D8 균주 처리는 유채의 생육을 촉진시키며 유채에 저항성을 유도하므로 친환경생물방제에 적용할 수 있을 것으로 생각된다.

The effect of two plant growth-promoting rhizobacteria (PGPR) on plant growth and systemic protection against soft rot disease and stem rot disease of canola (Brassica napus), caused by Erwinia carotovora and Sclerotinia sclerotiorum was investigated in a laboratory and a greenhouse. Selected PGPR strains C4 and D8 were treated to canola seeds by soaking. Strains C4 and D8 significantly not only increased plant height and root length about 74% and 40.3% and also reduced disease severity of soft rot disease by 80% by C4 and D8 respectively, compared to the control. Especially strain C4 showed antifungal activity against 6 fungal pathogens, S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Colletotrichum acutatum. In greenhouse experiment, the seed treatment of both of them increased plant height, leaf width and leaf length of canola plant to 19.5% and 24.9%, 11.3% and 15.3%, and 14.1% and 20.7% by C4 and D8, respectively, and reduced disease severity of S. sclerotiorium. These results indicate that these two PGPR strains can decrease disease severity and increased plant growth under greenhouse condition. Therefore, these two bacteria have a potential in controlling Sclerotinia stem rot of canola. These strains have to investigate under field condition to determine their role of antibiosis, induced systemic resistance and plant growth promotion on canola.

키워드

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