• The Korean Journal of Food And Nutrition
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• v.23 no.4
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• pp.582-587
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• 2010

본 연구에서는 녹두 유전자원의 지방산 함량을 신속 대량 검정하는 기술을 개발하여 유전자원 활용 및 육종 촉진에 기여하고자 하였다. 유전자원 평가에 적합한 신속하고 비파괴적인 지방산 함량 평가기술을 개발하기 위해 공시자원 1,125점의 녹두 종자를 종실상태와 분쇄한 분말상태로 근적외선분광분석기(NIR)를 이용하여 1,104~2,494 nm에서의 스펙트럼을 얻고 이들 중 스펙트럼이 중복되지 않는 원산지가 다양한 대표자원 106점을 선발하여 일반적인 방법으로 지방산 함량을 분석하고, 이 값과 NIR 스펙트럼 흡광도값 간의 상관분석을 위한 calibration set로 활용하였다. 그 결과 palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid 및 total fatty acid에 대한 NIR 흡광도와의 상관계수 $R^2$이 각각 0.74, 0.18, 0.12, 0.72, 0.48 및 0.78로 나타났고, 이들 중 $R^2$가 높은 검량식을 미지의 시료 10점으로 검증한 결과, palmitic, linoleic 및 total fatty acid에 대한 검증 상관계수 $R^2$이 0.96, 0.74, 0.81로 나타나, 다양한 녹두 유전자원의 지방산함량 신속 대량 예측에 유효하게 활용될 수 있는 것으로 나타났다. 한편, 공시된 녹두 유전자원 115점 중에서 자원번호 IT208075 자원은 저 지방산 자원($14.24\;mg\;g^{-1}$)으로 선발되었고, IT163279 자원은 고 지방산 자원($18.43\;mg\;g^{-1}$)으로 선발되어 향후 녹두작물의 성분육종에 유용할 것으로 생각된다.

• Current Research on Agriculture and Life Sciences
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• v.32 no.1
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• pp.10-17
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• 2014

This study presents an eco-friendly persimmon drying system to satisfy consumer preferences and provide a popular food for both the domestic and international markets. The most effective antimicrobial compounds were developed from a combination of plant extracts (18.18% clove buds, 9.90% cinnamon, 9.09% licorice, 4.55% cnidium, 4.55% seed of grapefruit, and 54.54% apple vinegar). The dried persimmons were evaluated as regards their moisture and sugar content, weight, hardness, and color value. During the drying process, the overall moisture content of the persimmons increased, along with the sugar content. The hardness was almost the same for each region and decreased on an average of 0.5~0.86 after 6 weeks. As regards the chromaticity, ${\Delta}E$ decreased during the drying process, while L-value became darker and a-value showed a dark red color over time.

• Journal of Plant Biotechnology
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• v.32 no.1
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• pp.31-35
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• 2005

To obtain regeneration system of onion, we analyzed the effects of 2,4-D and BA concentration on the embryogenic callus induction from mature embryos. The highest embryogenic callus induction ratio was shown on MS medium (Murashie and Skoog 1962) containing $2.5\;\cal{mg/L}\;or\;5\;\cal{mg/L}$ picloram after mature embryos were placed on medium. When induced callus were cultured on half strength of MS medium containing $1\;\cal{mg/L}$ Kinetin, the highest shoot formation ratio was observed on MS medium containing $1\;{mg/L}$ 2,4-D and $1\;{mg/L}$ BA. Embryogenic callus were cultured in MS liquid medium containing $1\;\ccal{mg/L}$ of 2,4-D and $1\;\cal{mg/L}$ BA. The suspension cultured cell clumps could be mass propagated. Embryogenic callus were friable, but non-embryogenic callus included a lot of moisture, hence the identification between embryogenic and non-embryogenic callus as easily achieved. When embryogenic callus as cultured on half strength of MS medium containing $1\;\cal{mg/L}$ Kinetin, shoots were induced. The whole plantlet was obtained on rooting medium containing $0.5\;\cal{mg/}$ of NAA.

• Journal of Plant Biotechnology
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• v.32 no.4
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• pp.257-262
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• 2005

Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic melon. Cotyledonary-node explants of melon (Cucumis melo L. cv. Super VIP) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 35S promoter-gus gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selective agent, and the binary vector (pPTN290) carrying with Ubiquitin promoter-GUS gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent, respectively. The maximum transformation efficiency (0.12%) was only obtained from the cotyledonary-node explants co-cultivated with EHA101 strain (pPTN289) on selection medium with 5 mg/L glufosinate and not produced a transgenic melon from the cotyledon or cotyledonary-node co-cultivated with other strains. Finally, five plants transformed showed the resistance in glufosinate antibiotic and the GUS positive response in leaf ($T_0$), flower ($T_0$), seeds ($T_1$) and plantlet ($T_1$). Southern blot analysis revealed that the gus gene integrated into each genome of transgenic melon.

• Journal of Plant Biotechnology
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• v.31 no.1
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• pp.43-48
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• 2004

As an initial step for future genetic manipulations to improve forage characteristics of Italian ryegrass (Lolium multiflorum Lam.), an efficient tissue culture system was established and the factors affecting plant regeneration were evaluated. MS medium containing 5mg/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency was observed at above 70％ when embryogenic calli induced were transferred to N6 medium supplemented with 1mg/L 2,4-D and 5mg/L BA. Among several basic media tested, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. 'Jeanne' showed the highest regeneration frequency of 73％. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.5
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• pp.405-412
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• 1992

Growth characters of dry seeded paddy rice was studied at different levels of nitrogen(N) split application (basal : immediately after irrigation : 2 weeks after irrigation: 25 days before heading: heading stage with 10 : 20 : 30 : 20 : 20%, 20 : 30 : 20 : 20 : 10%, 30 : 40 : 0 : 20 : 10%, and 100% basal of slow release urea) in tillage and no-tillage systems. On May 17 in 1990, 6kg /10a of dry seeds of a breeding line of Milyang 95 was broadcasted and covered with a power tiller. Between tillage and no-tillage systems yield and its components, lodging related characteristics, and contents of cellulose, hemicellulose and lignin of culm base were similar. Leaf area index at heading stage was higher in tillage, but lodging index was lower compared with those in no-tillage plot. Culm length, effective tiller ratio, culm base weight, 1000-grain weight, harvest index in slow release N applied plot were higher compared with those in other N treatments. Grain yield of rice among levels of N split applications was similar in tillage, but it was higher in slow release N applied plot in no-tillage if 1kg /10a of additional N was applied as urea at panicle formation stage. At early growth stage N concentration and N uptake were lower in the slow release N applied plot, but higher after heading stage compared with other N treatments. The number of tillers was lowest in slow release N applied plot during the tillering stage, but the number of panicles per hill was similar to other N treatments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.1
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• pp.60-65
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• 2017

This study was conducted to dentify the optimum plantlet type of 'Solara' potatoes (Solanum tuberosum L.) for growth in an aeroponics system. Plantlets of 'Solara' were transplanted on March 16, 2015 in a greenhouse, and growth and yield characteristics were investigated at 70 and 78 days after transplanting, respectively. Stem length was shorter in plantlet of 15-day-old stem cuttings and acclimatization of culture, and the stem length of plantlets of stem cuttings tended to increase with increasing stem cutting age. The fresh weight of plants was the highest in the plantlets of 40-day-old stem cuttings and the lowest in non-rooted stem cuttings and acclimatization of culture. The highest number of first stolons was obtained in 35-day-old stem cuttings. The number of second stolons was the highest in plantlets of 35-day-old stem cuttings, acclimatization of culture, and 30- day-old stem cuttings. The total number of tubers was higher in plantlets of 35-day-old stem cuttings and acclimatization of culture, and the number of tubers above 3 g was the highest in plantlets of 35-day-old stem cuttings. The weight of tubers above 3 g was the heaviest in plantlets of 35-day-old stem cuttings(1,947 g per 10 plants), followed by plantlets of 30-day-old stem cuttings. These results indicate that plantlets of 30 to 35-day-old stem cuttings could be the best for production of 'Solara' potato tubers in an aeroponics system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.2
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• pp.151-155
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• 2020

This study was conducted to determine the effects of the selection of an appropriate sowing method in on ginseng growth, and the results are as follows. SAS (semi-automatic sower), commonly used by direct seeding farms, provides fast operation speed but results in high labor cost, while (Hooper Seeder Sowing) offers fast operation speed and high germination rate, but leads to increased laborer's fatigue. Daily working area per sower was 6,611 ㎡ for a motor type, and the operation time to sow 3,305 ㎡ was 15 hours for broadcast planting, and 8 hours for SAS. The germination rate of 1-year-old ginseng according to direct seeding was 69.1% for the treatment plot under broadcast planting, followed by 66.4% and 67.3% for manual sower and hoop sower, respectively, and the germination rate of SAS was the lowest at 64.2%. In addition, there were no differences in shoot and root growth of 1-year-old ginseng among the sowing methods. Therefore, the sowing method can be chosen depending on the economic size of ginseng farmers. That broadcast sowing (BS) is appropriate for small scale farmers, while motor-driven seeder sowing (MDS) for direct seeding is more suitable for large scale farmers.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.547-552
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• 1993

Soybean nuclear extracts and S-100 were prepared to examine the soybean embryo factors which bind to the upstream region of soybean ${\beta}-conglycinin$ ${\alpha}'$ subunit gene. SEF3(soybean embryo factor 3), which is presumed to be a trans-acting factor for the expression of the gene, was detected in gel mobility shift assay using the DNA probe containing two AACCCA hexanucleotides. DNA probe containing CATGCAT or AACACA was used to find any other soybean embryo factor interacting with the upstream region of ${\beta}-Conglycinin$ ${\alpha}'$ subunit gene. It was found that there was no common DNA binding protein detected both in nuclear extracts and S-100. The relative levels of SEF3 binding activity both in nuclear extracts and S-100 of maturing soybean seeds were determined. SEF3 activity of nuclear extracts was first detected around 20 days after pollination and significantly increased around 32 days after pollination.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.1
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• pp.53-56
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• 2007

A new orchardgrass (Dactylis glomerata L.) variety named "Kordione" has developed by the National Livestock Research Institute (NLRI) in 2005. To develope a new variety of orchardgrass, 5 superior clones were selected and polycrossed for seed production. Various agronomic growth paramaters and forage production of "Kordione" were examined at Suwon from 2001 to 2002. During 2003-2005, regional trials were conducted at Suwon, Pyungchang, Youngju, Iksan, and Jeju. "Kordione" showed a medium type growth habit in fall and spring, and short in length of flag leaf and upper internode. Plant height of "Kordione" was similar to that of standard variety, "Ambassador" and heading date was 2 days later than 10th May compared to Ambassador. "Kordione" showed enhanced winter hardiness, lodging tolerance and disease resistance compared to Ambassador. In addition, dry matter yield (12,081 kg/ha) of "Kordione" was also higher (11%) than that of the Amabassador. However, nutritive value was similar in both varieties.