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http://dx.doi.org/10.5010/JPB.2004.31.1.043

Efficient Plant Regeneration from Mature Seed-derived Callus of Italian ryegrass (Lolium multiflorum Lam.)  

Woo, Hyun-Sook (Division of Applide Life Science, Gyeongsang National University)
Lee, Sang-Hoon (Division of Applide Life Science, Gyeongsang National University)
Lee, Dong-Gi (Division of Applide Life Science, Gyeongsang National University)
Kim, Jin-Soo (Division of Applide Life Science, Gyeongsang National University)
Won, Sung-Hye (Institute of Agricultural Science & Technology, Kyungpook National University)
Lee, Byung-Hyun (Division of Applide Life Science, Gyeongsang National University)
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 43-48 More about this Journal
Abstract
As an initial step for future genetic manipulations to improve forage characteristics of Italian ryegrass (Lolium multiflorum Lam.), an efficient tissue culture system was established and the factors affecting plant regeneration were evaluated. MS medium containing 5mg/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency was observed at above 70％ when embryogenic calli induced were transferred to N6 medium supplemented with 1mg/L 2,4-D and 5mg/L BA. Among several basic media tested, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. 'Jeanne' showed the highest regeneration frequency of 73％. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.
Keywords
Forage crop; Italian ryegrass; Lolium multiflorum; plant regeneration;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 McKersie BD (1997) Improving forage production systems using biotechnolgy. In: McKersie BD and Brown DCW (eds), Biotechnology in Agriculture Series, No. 17, CAB International, Wallingford, pp 3-21
2 Murashige T, Skoog F (1962) A revise medium for rapid growth andbioassays with tobacco tissue cultures. Physiol Plant 15:473-497   DOI
3 Park BH, Park BS, Kang JH (1987) A comparison between diploidand tetraploid cultivars of Lotium muttiftorum Lam. J Kor Grassland Sci7:135-139
4 Rim YW, Kim KY, Choi KJ, Sung BR, Shin JS (2000) Callus induction from seeds of Italian ryegrass and plant regeneration. J Kor Grassland Sci 20: 25-30
5 Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199-204   DOI
6 Spangenberg G, Wang ZY, Potrykus (1998) Biotechnology in forage and turf grass improvement. In: Frankel et al (eds), Monographs on theoretical and applied genetics, Vol. 23, Springer Verlag, Heidelberg, pp 192-210
7 Van Wijk AJP, Boonman JG, Rumball W (1993) Achievements and prospectives in the breeding of forage grasses and legumes. In: Baker MJ (eds), Grasslands for our world, SIR, Wellington, pp 116-120
8 Creemers-Molenaar J, Loeffen JPM, Van der Valk P (1988) The effect of 2,4-dichlophenoxyacetic acid and donor plant environment on plant regeneration from immature inflorescence derived callus of Lolium perenne L. and Lolium multiflorum L. Plant Sci 57: 165-172   DOI   ScienceOn
9 Vasil V, Vasil IK (1984) Induction and maintanance of embryogenic callus cultures of Gramineae. In: Vasil IK (eds), Cell culture and somatic cell genetics of plants, Vol 1, Academic Press, Orlando, pp 36-42
10 Wang ZY, Nagel J, Potrykus I, Spangenberg G (1993) Plants from cell suspension-derived protoplasts in Lolium species. Plant Sci 94: 179-193   DOI   ScienceOn
11 Ye X, Wang ZY, Wu X, Potrykus I, Spangenberg G (1997) Transgenic Italian ryegrass (Lolium multiflorum) Plants from microprojectile bombardment of embryogenic suspension cells. Plant Cell Rep 16: 379-384   DOI
12 Dale PJ (1980) Embryoids from cultured immature embryos of Lolium multiflorum. Pflanzen Physiol 100:73-77   DOI
13 Bai Y, Qu R (2000) An evaluation on callus induction and plant regeneration of 25 turf-type tall fescue (Festuca arundinacea Schreb.) cultivars. Grass Forage Sci 55:326-330   DOI   ScienceOn
14 Choi GJ, Rim VW, Kim KY, Choi SH, Sung BR, Kim WH, Shin DE, Lim YC (2000) A cold-tolerant and high-yielding Italian ryegrass (Lolium multiflorum L.) new variety 'Hwasan 101'. J Kor Grassl Sci 20:1-6
15 Chu CC, Wang CS, Sun CC, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic 18:659-668
16 Dale PJ, Thomas E, Brettell RIS, Wemicke W (1981) Embryogenesis from cultured immature inflorescences and nodes of Lolium multiflorum. Plant Cell Tiss Org Cult 1:47-55   DOI
17 Hides DH Kute CA, Marshall AH (1993) Seed development and seed yield potential of Italian ryegrass (Lolium multiflorum Lam.) populations. Grass and Forage Sci 48:181-188   DOI   ScienceOn
18 Dalton SJ, Betanny AJE, Timms E, Morris P (1999) Co-transformed, diploid Lolium perenne, Lolium. multifIorum and Lolium temulentum plants produced by microprojectile bombardment. Plant Cell Rep 18: 721-726   DOI   ScienceOn
19 Forster JW, Spangenberg G (1999) Forage and turf grass biotechnology: principles, methods and prospects. In: Setlow JK (eds), Genetic engineering: principles and methods, Vol 21, Kluwer Academic Publishers, New York, pp 191-237
20 Gnffin JD, Dibble MS (1995) High frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L).Plant Cell Rep 14:721-724
21 Isselstein J (1993) Influence of slight shading, sward density and nitrogen fertilization on yield and nutritive value of Lolium multiflorum Lam. J Agro and Crop Sci 170: 341-347   DOI   ScienceOn
22 Lee SH, Lee DG, Kim JS, Lee BH (2003) High-frequency plant regeneration from mature seed-derived callus culture of orchardgrass. Kor J Plant Biotechnol 30: 341-346   DOI   ScienceOn
23 Lee HS, Kang KM, Jo J (2001) factors affecting plant regeneration from seed-derived calli in Italian ryegrass (Lolium multiflorum Lam). Kor J Plant Tiss Cult 28: 323-328
24 Kumlehn J, Nitzsche W (1995) Plant regeneration from isolated ovules of Italian ryegrass (Lolium multiflorum Lam.): effect of 2,4- dichlorophenoxyacetic acid and different cytokinins supplemented to the ovule culture medium. Plant Sci 111:107-116   DOI   ScienceOn