Browse > Article
http://dx.doi.org/10.5010/JPB.2005.32.4.257

Production of Transgenic Melon from the Cultures of Cotyledonary-Node Explant Using Agrobacterium-Mediated Transformation  

Cho Mi-Ae (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Song Yun-Mi (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Park Yun-Ok (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Ko Suck-Min (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Min Sung-Ran (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Liu Jang-Ryol (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee Jun-Haeng (Department of Medicinal Plant Resources, Nambu University)
Choi Pil-Son (Department of Medicinal Plant Resources, Nambu University)
Publication Information
Journal of Plant Biotechnology / v.32, no.4, 2005 , pp. 257-262 More about this Journal
Abstract
Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic melon. Cotyledonary-node explants of melon (Cucumis melo L. cv. Super VIP) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 35S promoter-gus gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selective agent, and the binary vector (pPTN290) carrying with Ubiquitin promoter-GUS gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent, respectively. The maximum transformation efficiency (0.12%) was only obtained from the cotyledonary-node explants co-cultivated with EHA101 strain (pPTN289) on selection medium with 5 mg/L glufosinate and not produced a transgenic melon from the cotyledon or cotyledonary-node co-cultivated with other strains. Finally, five plants transformed showed the resistance in glufosinate antibiotic and the GUS positive response in leaf ($T_0$), flower ($T_0$), seeds ($T_1$) and plantlet ($T_1$). Southern blot analysis revealed that the gus gene integrated into each genome of transgenic melon.
Keywords
Agrobacterium strains; ${\beta}$-glucuronidase (GUS); transgenic melon;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15 : 473-497   DOI
2 Nishibayashi S, Kayakawa T, Nakajima T, Suzuki M, Kaneko H (1996) CMV protection in transgenic cucumber plants with an introduced CMV-O cp gene. Theor Appl Genet 93: 672-678   DOI   ScienceOn
3 Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503-512   DOI
4 Olhoft PM, Somers DA (2001) L-cysteine increase Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells. Plant Cell Rep 20: 706-711   DOI
5 Paz MM, Shou H, Guo Z, Zhang Z, Banerjee AK, Wang K (2004) Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant. Euphytica 136: 167-179   DOI
6 Shelp BJ, Swanton CJ, Hall JC (1992) Glufosinate (phosphinothricin) mobility in young soybean shoots. J Plant Physiol 139: 626-628   DOI
7 Simmonds DH, Donaldson PA (2000) Genotype screening for proliferative embryogenesis and biolistic transformation of short-season soybean genotypes. Plant Cell Rep 19: 485-490   DOI
8 Somers DA, Samac DA, Olhoft PM (2003) Recent advances in legume transformation. Plant Physiol 131: 892-899   DOI   ScienceOn
9 Tricoli DM, Carney KJ, Russell PF, Quemada HD, McMaster RJ, Reynolds JF, Deng RZ (2002) Transgenic plants expressing DNA containing a plurality of genes to impart virus resistance. US patent 6,337,431
10 Zhang Z, Xing A, Staswick P, Clemente TE (1999) The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean. Plant Cell Tiss Org Cult 56 : 37-46   DOI   ScienceOn
11 Gaba V, Zelcer A, Gal-On A (2004) Cucurbit biotechnology-the importance of virus resistance. In Vitro Cell Dev Biol Plant 40: 346-358   DOI   ScienceOn
12 Gray DJ, McColley DW, Compton ME (1993) High frequency somatic embryogenesis from quiescent seed cotyledons of Cucumis melo cultivars. J Am Soc Hort Sci 118: 425-432
13 Guis M, Ben Amor M, Latche A, Peche JC, Roustan JP (2000) A reliable method for the transformation of Cantaloupe Charentais melon (Cucumis melo L. var. cantalupensis) leading to a majority of diploid regenerants. Sci Hort 84: 91-99   DOI   ScienceOn
14 Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusion: ${\beta}-glucuronidase$ as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901-3907
15 Lee SH, Shon YG, Lee SI, Kim CY, Koo JC , Lim CO, Choi YJ, Han CD, Chung CH, Choe ZR, Cho MJ (1999) Cultivar variability in the Agrobacterium-rice cell interaction and plant regeneration. Physiol Plant 107: 338-340   DOI   ScienceOn
16 Meurer CA, Dinkins RD, Collins GB (1998) Factors affecting soybean cotyledonary node transformation. Plant Cell Rep 18: 180-186   DOI
17 Boyhan GJ, Norton D, Jacobsen BJ, Abrahams BR (1992) Evaluation of watermelon and related germplasm for resistance to zucchini yellow mosaic virus. Plant Dis 76: 251-252   DOI
18 Muller B, Zumdick A, Schuphan I, Schmidt B (2001) Metabolism of the herbicide glufosinate ammonium in plant cell cultures of transgenic and non-transgenic sugarbeet, carrot, purple foxglove and thorn apple. Pest Manag Sci 57: 46-56   DOI   ScienceOn
19 Fang G, Grumet R (1990) Agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants. Plant Cell Rep 9: 160-164
20 Finer KR, Finer JJ (2000) Use of Agrobacterium expressing green fluorescent protein to evaluate colonization of sonication-assited Agrobacterium-mediated transformation-treated soybean cotyledons. Lett Appl Microbiol 30: 406-410.   DOI   ScienceOn
21 Cho MA, Choi DW, Liu JR , Clemente T, Choi PS (2004) Development of transgenic soybean using Agrobacterium tumefaciens. Kor J Plant Biotechnol 31: 255-259   과학기술학회마을   DOI   ScienceOn
22 Cho MA, Song YM, Park YO, Ko SM, Min SR, Liu JR, Choi PS (2005a) The use of glufosinate as a selective marker for the transformation of cucumber (Cucumis sativus L.). Kor J Plant Biotechnol 32: 161-165   과학기술학회마을   DOI   ScienceOn
23 Cho MA, Song YM, Liu JR, Choi PS (2005b) Production of transgenic watermelon by Agrobacterium tumefaciens-mediated transformation. Kor J Plant Biotechnol (submitted)
24 Choi PS, Soh WY, Cho DY, Liu JR (1994) High frequency somatic embryogenesis and plant regeneration in seedling explant cultures of melon (Cucumis melo L .). Kor J Plant Biotechnol 21: 1-5   과학기술학회마을
25 Dong JZ, Yang MZ, Jia SR, Chua NH (1991) Transformation of melon (Cucumis melo L.) and expression from the cauliflower mosaic virus 35s promoter in transgenic melon. Bio/Tech 9: 858-863   DOI
26 Clemente T, LaValle BJ, Howe AR, Ward DC, Rozman RJ, Hunte PE, Broyles DL, Kasten DS, Hinchee MA (2000) progeny analysis of glyphosate selected transgenic soybean derived from Agrobacterium-mediated transformation. Crop Sci 40: 797-803   DOI
27 Curuk BS, Cetiner S, Elman C, Xia X, Wang Y, Yeheskel A, Zilberstein L, Perl-Treves R, Watad AA, Gaba V (2005) Transformation of recalcitrant melon (Cucumis melo L.) cultivars is facilitated by wounding with carborundum. Eng Life Sci 5: 169-176   DOI   ScienceOn
28 Dellaporta SL, Wood J, Hicks JB (1985) Maize DNA miniprep. In: Malmberg R, Messing J, Sussex (eds), Molecular Biology of Plants: A laboratory Course Manual, Cold Spring Harbor, New York, pp 36-37
29 Bauer N, Levanic DL, Mihaljevic S, Jelaska S (2002) Genetic transformation of Coleus blumei Benth. using Agrobacterium. Food Technol Biotechnol 40: 163-169