• Journal of Plant Biotechnology
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• v.41 no.3
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• pp.107-115
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• 2014

Vegetable oils (triacylglycerols) produced mainly in seeds of plants are used for valuable foods that supply essential fatty acids for humans as well as industrial raw materials and biofuel production. As the demanding for vegetable oils has increased, plant metabolic engineering to produce triacylglycerols in biomass such as leaves has been considered and explored for alternative source of vegetable oils. Leaves are genetically programmed to supply the fixed carbon by photosynthesis to other organs for plant development and growth. Therefore, in order to produce and accumulate triacylglycerols in leaves, one should take account of multiple metabolic pathways such as carbon flux, competition of carbohydrate and fatty acid biosynthesis, and triacylglycerols turnover in leaves. The recent metabolic engineering strategy has showed potential in which the co-expression of three genes WRINKLED1, DGAT1, and OLEOSIN involved in the critical step for increasing the fatty acid synthesis, accumulating triacylglycerols, and protecting triacylglycerols, respectively produced higher amount of vegetable oils in leaves. Developing of genetically engineered plants producing vegetable oil in biomass at non-agricultural lands will be promising to the future success of the field.

• Journal of Korean Society of Forest Science
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• v.85 no.2
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• pp.238-243
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• 1996

Three half-sib families of Betula platyphylla var. japonica had been introduced from Japan and tested for their growth potentiality by comparing with control trees originating from previously introduced and cultivated stands of unknown seed origin. Two of the three introduced family groups from Japan showed superior tree growth in both diameter and height to control one up to the age of four. But only one family group remained superior to the control trees at age six. It appeared from the results that Betula platyphylla var. japonica families, introduced from Japan, can be directly used for the production of planting stocks for commercial planting and/or as parental trees for the production of intra- and interspecific hybrids.

• KSBB Journal
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• v.9 no.3
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• pp.299-305
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• 1994

Taxol contents in various parts of 15 years old yew tree were determined. The descending order of taxol content per unit mass was stem bark, root bark, needle and seed. In the seed, that order was seed coat, embryo and endosperm. The total amount of taxol extractable from a 15 years old yew tree was 1.68 gram. This amount was distributed in needle, stem bark, root bark and seeds as 48.0, 23.8, 27.9 and 0.4%, respectively. Altitudinal variation of taxol content was also observed. More taxol was observed in yew trees grown at high altitude over 1000m above sea level. Calli and suspension cultures were induced from various yew trees. The presence of taxo] in cultured cells was established bv HPLC. The taxol content in cultured cells were different according to the source of explants. These results may be useful for the goal of large scale taxol production by cultured yew tree cells.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.149-157
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• 2019

This study interested on management of broodstock and the effect of raise the temperature for promotion of maturity in Tresus keenae. For management of broodstock, we performed indoor and outdoor culture. In addition, broodstock was put in exposure and immersion condition. This experimental design is aimed at knowing the optimal management of broodstock. In addition, we investigated the promotion of maturity, growth and survival rate when raise the temperature of the breeding seawater of broodstock in winter season. Result that, the management of broodstock was shown to be more effective obesity and survival rate in outdoor culture than indoor culture. In addition, there is no anthropogenic food supply in management and it is easy to management because it is kept under natural environmental conditions. Therefore, It is considered appropriate to management to outdoor culture. In raise the temperature, survival rate was lower in the experimental group than in the control group. However, obesity was significantly increased compared with the control group. Also, gonad showed that the sex maturity was promoted. If sex maturation is not developed due to seasonal water temperature, the promotion of sexual maturity through heating is considered to be an effective method in terms of artificial seed production.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.100-106
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• 1995

This study was conducted to find the factors affecting somatic embryogenesis in suspension culture of Rehmania glutinosa and investigate the possibility of artificial seed production by encapsulation of somatic embryos. Linsmeier-Skoog medium was appeared as proper for somatic embryogenesis. Sucrose with $3{\sim}5%$ as carbon sources was good for somatic embryogenesis, and both ammonium and nitrate nitrogen were necesary for normal somatic embryo production. BA with NAA or kinetin with NAA were better than the use of cytokinin alone for both somatic embryogenesis and numbers of somatic embryos. $AgNO_3$ as protectant for vitrification of seedlings in vitro culture had no harmful effect on somatic embryos. Sphericity of encapsulated seeds was good at 3% gel of sodium alginate but germination was better at 2.5% sodium alginate level. Artificial seeds were germinated and developed normal shoots and roots under in vitro condition.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.392-402
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• 1987

To know the effect of presowing seed soaking with some growth substances (kinetin, IAA, ethrel and salicylic acid) on vegetative growth and yield contributing parameters in soybean [Glycine max (L.) Merrill] the investigation was undertaken. The salient features from the study are;-All the treatments showed the enhancement effect on vegetative growth, viz. seediling emergence, germination percentage, plant height, number of leaves and branches per plant as compared with control, whereas salicylic acid delayed the seedling emergence process and lowered the germination percentage.-Nodule initiation, number of nodules and nodule weight were hastened and increased respectively under all the treatments whereas IAA showed an opposite effect on all the parameters at the early stages of nodule development.-Kinetin, IAA and ethrel showed the significant enhancement effect on the levels of biochemical parameters throughout the time of seed development whereas salicylic acid and water showed the tendency similar to that of control.-Yield and its components, viz. number of flowers, pods and seeds per plant were significant increased under all the treatments but they didn't show any significant enhancement effect on number of seeds per pod and pod setting rate. One hundred seed weight was lower under all the treatments, indicating the dilution effect resulting from incapability of increased number and size of source to provide the increased size of sink with assimilates.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.4
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• pp.342-351
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• 1990

Experiments were conducted to find out the possibilities of producing good quality seed of summer type soybean at Chuncheon, 74m above sea level and Jinboo, 600m above sea level in 1987. Local variety, Cheonan Jaelae was used. Seeds were sown June 25 harvested five times from 35 days after flowering with five days interval to 55 days. Vegetative period was longer in the early planting and high cool area, Jinboo, which resulted longer stem length than the late planting and in the plain area, Chuncheon. Pod number and 100 grain weight were shown more and heavier in Jinboo. Earlier planting and later harvesting resulted more pod number and heaveier 100grain weight. Grain yield was heigher in Jinboo, and earlier plainting shown more grain yield and its difference was greater in Jinboo. Long grain filling duration resulted more grain yield. Unripened seeds were produced more by earlier planting with earlier harvesting and earlier planting with late harvesting showed the high rates of pod and stem blight (Diaporthe Phaseolorum). Seed With high moisture content by early harvesting showed more rotten seeds in all planting dates production was more advantageous in the high cool area, Jinboo. Optimum planting date was between May 15 and June 5. Harvesting was desirable about 45-50 days after flowering with heigher germination rate seed yield.

• Proceedings of the Botanical Society of Korea Conference
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• 1995.07a
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• pp.109-130
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• 1995

지난 반세기간에 우리나라에 채소 육종은 주요 채소의 주년공급을 가능하게 하였으며, 토지 생산성의 향상, 상품화율의 증대, 품질의 향상 등의 면에서도 괄목할 만한 성과를 거두었고 인공교잡은 물론이고 웅성불임성과 자가불화합성의 활용에 의한 1대잡종 품종의 일반화로 채소 산업의 발전에 크게 기여하였다. 앞으로는 기왕의 업적을 심화시키는 한편, 생산비를 절감하기 위한 생력화, 기계화 재배용 품종 및 내제초제성 품종의 개발 환경보호 및 식품안정성의 확보를 위한 내병층성 품종 개발, 수출시장과 다양화하는 국내의 시장기호에 대응하고 가공 식품의 표준화된 품질관리를 지원할 수 있도록 품질 면에서의 개량과 신작물 또는 신생태형 품종의 개발에도 더욱 노력이 필요하다. 이러한 육종목표를 달성하기 위한 유전자원의 확보는 더욱 어려워질 것이며 유전 양식이 복잡하고, 환경요인의 작용이 상대적으로 크기 때문에 전통적인 육종 방법만으로는 목표달성에 필요한 인적, 물적, 시간적 소요가 훨씬 증가될 전망이다. 유전변이의 창성 및 확대, 유용 대립인자의 도입, 동정 및 선발, 그리고 종자생산을 위한 자가 불화합성 및 웅성불임성과 개화·수정 관련 유전인자의 발현 조절에 분자유전학의 보완적 역할이 기대되며 이렇게 되면 전통육종과 분자유전학간의 잡종강세로 품종 개량의 효율은 크게 높아질 것이다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.4
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• pp.392-399
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• 1988

The experiments were conducted to establish the effective barley haploid production system using interspecific crosses. Three spring barley cultivars. Bruce. Klages and Rodeo were used for this experiment. 1, 687 florets of three barley cultivars were crossed with bulbosum pollens. 1, 079 seeds were harvested and obtained 834 embryos so that seed set rate and embryo production rate were 64% and 77%, respectively. IAA effect was superior to NAA for root development and 1 ppm concentration of IAA gave the best result among five concentrations; 0ppm, 1ppm., 5ppm, 10ppm and 30ppm.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.123-123
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• 2022

최근 전 세계적으로 대마를 활용한 산업화가 활발하게 이루어지고 있다. 우리나라도 2019년부터 의료목적에 한하여 대마 의약품이 허용되었으나, 현행법 상 관련 의약품의 국내 생산은 불가능하기 때문에 전량 수입에 의존하고 있다. 현재, 대마와 관련하여 식의약소재로 산업화가 가능한 부분은 껍질(포엽과 외종피)이 완전히 제거된 종자 뿐이다. 결국, 대마의 사용이 합법화 되고 있는 미국, 캐나다에 비해 식의약산업에 있어서의 활용 범위가 좁다. 이에 본 연구에서는 대마 종자를 이용하여 일정 기간 싹을 틔워 식품으로 활용할 수 있는 과학적 근거를 마련하고자 하였다. 사전 연구를 통해 재배조건에 따른 성분의 변화, 세포독성 등이 검토되었고, 이를 기반으로 새싹대마의 대량생산연구 및 안전성·독성 검토, 생리활성 연구를 계획·수행하고 있다. 다양한 대마 소재를 발굴하고, 이를 산업화할 수 있는 첫걸음으로서 의의를 가진다.