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Identification of Chromosomal Band Markers of the Korean Native Chicken (한국재래계의 염색체 분염 표지 분석)

  • Baik, K. H.;Lee, C. Y.;Sang, B. D.;Choi, C. H.;Kim, H. K.;Sohn, S. H.
    • Journal of Animal Science and Technology
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    • v.45 no.1
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    • pp.1-12
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    • 2003
  • The present study was carried out to establish the standard karyotype of the Korean Native Chicken and to find their chromosomal band markers using high-resolution banding technique. Chromosome analysis was performed on early chick embryos following in vitro culture of fertilized eggs of the yellow-brown and the red-brown lines of the Korean Native Chicken which had been established at National Livestock Research Institute. The high-resolution banding of the chromosome was achieved by treating the embryos with ethidium bromide and colchicine during culture. On GTG-banding, the Korean Native Chicken exhibited a typical chick banding pattern in all the macrochromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Chicken were virtually identical to those of White Leghorn and International System for Standardized Avian Karyotypes(ISSAK). However, the lengths and G-band numbers of the Korean Native Chicken macrochromosomes were greater than those of White Leghorn and ISSAK. Especially in chromosomes 1 and Z, the Korean Native Chicken exhibited more separated bands in compared with ISSAK. In C-banding patterns, although a lot of observed cells had C-band polymorphic patterns, almost the Korean Native Chicken macrochromosomes had heterochromatic C-band on centromeres and/or near terminal part. However, the heterochromatic C-band was constantly observed at the end of q-arm of Z chromosomes and on the whole W chromosome. In addition, the Korean Native Chicken exhibited distinctive heteromorphic patterns of C-bands on the centromere of chromosome 3 and at the end of q-arm of Z chromosome between homologous chromosomes.

Improved Vapor Recognition in Electronic Nose (E-Nose) System by Using the Time-Profile of Sensor Array Response (센서 응답의 Time-Profile 을 이용한 전자 후각 (E-Nose) 시스템의 Vapor 인식 성능 향상)

  • Yoon Seok, Yang
    • Journal of Biomedical Engineering Research
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    • v.25 no.5
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    • pp.329-334
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    • 2004
  • The electronic nose (E-nose) recently finds its applications in medical diagnosis, specifically on detection of diabetes, pulmonary or gastrointestinal problem, or infections by examining odors in the breath or tissues with its odor characterizing ability. The odor recognition performance of E-nose can be improved by manipulating the sensor array responses of vapors in time-profile forms. The different chemical interactions between the sensor materials and the volatile organic compounds (VOC's) leave unique marks in the signal profiles giving more information than collection of the conventional piecemal features, i.e., maximum sensitivity, signal slopes, rising time. In this study, to use them in vapor recognition task conveniently, a novel time-profile method was proposed, which is adopted from digital image pattern matching. The degrees of matching between 8 different vapors were evaluated by using the proposed method. The test vapors are measured by the silicon-based gas sensor array with 16 CB-polymer composites installed in membrane structure. The results by the proposed method showed clear discrimination of vapor species than by the conventional method.

Seismic Properties Study of Gas Hydrate in Deep Sea using Numerical Modeling Technique (수치 모델링 기술을 이용한 심해 가스 하이드레이트의 탄성파 특성 연구)

  • Shin, Sung-Ryul;Yeo, Eun-Min;Kim, Chan-Su;Park, Keun-Pil;Lee, Ho-Young;Kim, Young-Jun
    • Geophysics and Geophysical Exploration
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    • v.9 no.2
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    • pp.139-147
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    • 2006
  • We had conducted a numerical modeling to investigate seismic properties of gas hydrate with field parameters acquired over the East sea in 1998. We used a 2-D staggered grid finite difference method to generate synthetic elastic seismograms for multi-channel seismic survey, OBC (Ocean Bottom Cable) survey and VCS (Vertical Cable Seismic) survey. The results of this study showed that the method using staggered grid yielded stable results and could be used to seismic imaging. We could find out the high amplitude anomaly and the phase reversal phenomenon of reflection wave at interface between the gas hydrate layer and free gas layer such a BSR (Bottom Simulating Reflector) which is the evidence for existence of gas hydrate in seismic reflection data. And we computed the reflection coefficients at the incident angles corresponding to offset distance with the synthetic seismograms. The reflection coefficients acquired from the numerical modeling were nearly consistent with the reflection coefficient computed by Shuey's equation.

Moment Magnitude Determination Using P wave of Broadband Data (광대역 지진자료의 P파를 이용한 모멘트 규모 결정)

  • Hwang, Eui-Hong;Lee, Woo-Dong;Jo, Bong-Gon;Jo, Beom-Jun
    • Journal of the Korean Geophysical Society
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    • v.10 no.1
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    • pp.1-12
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    • 2007
  • A method to quickly estimate broadband moment magnitudes (Mwp) to warn regional and teleseismic tsunamigenic earthquakes is tested for application of the method to the different seismic observation environment. In this study, the Mwp is calculated by integrating far-field P-wave or pP-wave of vertical component of displacement seismograms in time domain from earthquakes, having magnitude greater than 5.0 and occurred in and around the Korean peninsula from 2000 to 2006. We carefully set up the size of the time window for the computations to exclude S wave phases and other phases following after the P wave phase. The P wave velocities and the densities from the averaged Korean crustal model are used in the computations. Instrumental correction was performed to remove dependency on the seismograph. The Mwp after the instrumental correction is about 0.1 greater than the Mwp before the correction. The comparison of our results to the those of foreign agencies such as JMA and Havard CMT catalogues shows a higher degree of similarity. Thus our results provide an effective tool to estimate the earthquake size, as well as to issue the necessary information to a tsunami warning system when the effective earthquake occurs around the peninsula.

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Regulation of Pipernonaline on Biological Functions of Human Prostate Cancer Cells Based on Microarray Analysis (Microarray를 이용한 pipernonaline의 인간 전립선 암세포에 대한 기능 조절 분석)

  • Kim, Sang-Hun;Kim, Kwang-Youn;Yu, Sun-Nyoung;Park, Seul-Ki;Kwak, In-Seok;Rhee, Moon-Soo;Bang, Byung-Ho;Chun, Sung-Sik;Ahn, Soon-Cheol
    • Journal of Life Science
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    • v.22 no.11
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    • pp.1552-1557
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    • 2012
  • It has been reported that pipernonaline isolated from Piper longum Linn. has a wide biochemical and pharmacological effect, including antitumor activity in prostate cancer PC-3 cells. However, its mechanism and expression pattern of many genes involved in biological functions are not clearly understood. To perform the gene expression study in PC-3 cells treated with pipernonaline, a cDNA microarray chip composed of 44,000 human cDNA probes was used. As a result, cell cycle-related genes, apoptosis-related genes, and cell proliferation/growth-related genes have been identified in gene ontology of the DAVID database. These results suggest that pipernonaline has antitumor activity by regulating the expression pattern of genes involved in biological signaling pathway in prostate cancer PC-3 cells. Further, additional analysis of these microarray data can be a useful tool to identify the mechanism and discovery of novel genes in cancer therapy.

EFFECT OF EUGENOL AND CAPSAICIN ON THE VOLTAGE-DEPENDENT ION CHANNELS OF TRIGEMINAL AFFERENTS (삼차신경 일차구심 뉴런의 전압의존성 이온통로에 대한 capsaicin과 eugenol의 작용)

  • Kim, Ju-Youn;Park, Sang-Jin;Choi, Gi-Woon;Choi, Ho-Young
    • Restorative Dentistry and Endodontics
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    • v.25 no.3
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    • pp.407-420
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    • 2000
  • 삼차신경절의 뉴런이 구강악안면영역에서의 촉각, 입각, 온도각 및 통각 등 다양한 감각을 중추신경계로 전달하는 역할을 하는 것은 주지의 사실이다. 이러한 신경전달에 있어서 이온통로는 감각정보를 전달하는데 핵심적인 역할을 수행하며 특히 소디움 통로는 활동전위의 발생에 중요하다. 소디움 통로는 tetrodotoxin-sensitive(TTX-s) 및 tetrodotoxin-resistant(TTX-r) 통로로 나누어지는 데 이 중 TTX-r 통로에 발생되는 tetrodotoxin-resistant sodium current(TTX-r $I_{Na}$)는 capsaicin에 민감한 일차구심신경세포에서 유해자극에 의해 통각신호를 발생시키고 전달하는데 중요하다. 또한 칼슘 통로는 시냅스 전도에 있어서 필수적인 역할을 수행하고 있다 한편 치과영역에서 치수의 진정 목적으로 eugenol이 흔히 사용되고 있다. 그러나 eugenol의 그 작용 기전에 대해서 현재까지 이온 통로에 대한 상세한 결과가 없는 실정이며 최근의 보고에 의하면 eugenol이 capsaicin 수용기를 통하여 감각신경에 대한 억제작용을 나타낸다고 한다. 따라서 본 실험은 eugenol과 capsaicin이 흰쥐의 삼차신경절의 TTX-r $I_{Na}$와 칼슘통로에 어떠한 영향을 미치는지를 알아보고 eugenol이 capsaicin 수용기를 통하여 작용하는지를 검증하고자 시행되었다. 삼차신경절 뉴런은 100~150g의 흰쥐의 삼차신경절로부터 외과적으로 절제하여 통법의 화학적 및 기계적 처리를 통해 단일세포로 분리하였고 이를 whole-cell patch clamp 방법을 이용하여 시행한 바 다음과 같은 결론을 얻었다. 1. 1mM의 dugenol은 흰쥐 삼차신경절 뉴런의 TTX-r $I_{Na}$와 HVA $I_{Ca}$를 억제하였다. 2. $1{\mu}m$의 capsaicin은 흰쥐 삼차신경절 뉴런의 TTX-r $I_{Na}$와 HVA $I_{Ca}$를 억제하였다. 3. Capsazepine은 capsaicin의 HVA $I_{Ca}$에 대한 억제작용을 차단하였다. 4. Capsazepine은 capsaicin의 HVA $I_{Ca}$에 대한 억제작용을 차단하지 못하였다. 결론적으로 eugenol과 capsaicin은 tetrodotoxin-resistant sodium current(TTX-r $I_{Na}$)와 high voltage-activated calcium current(HVA $I_{Ca}$)를 모두 억제하는 것으로 나타났으며, 이러한 작용이 통각의 발생과 시냅스 전달과정을 차단하여 치수 진정 목적으로 많이 사용하는 eugenol의 작용기전으로 판단된다. 한편 capsaicin의 길항제인 capsazepine을 전처치하였을 때에도 eugenol의 HVA $I_{Ca}$에 대한 억제효과는 변화가 없었다. 이와같은 결과로 보아 HVA $I_{Ca}$에 관한 한 eugenol은 capsaicin 수용기를 통하여 나타나지 않는 것으로 사료된다.

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A study on behavior response of child by emotion coaching of teacher based on emotional recognition technology (감성인식기술 기반 교사의 감정코칭이 유아에게 미치는 반응 연구)

  • Choi, Moon Jung;Whang, Min-Cheol
    • Journal of the Korea Convergence Society
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    • v.8 no.7
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    • pp.323-330
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    • 2017
  • Emotion in early childhood has been observed to make an important effect on behavioral development. The teacher has coached to develop good behavior based on considering emotional response rather than rational response. This study was to determine significance of emotional coaching for behavior development according emotion recognized by non-verbal measurement system developed specially in this study. The participants were 44 people and were asked to study in four experimental situation. The experiment was designed to four situation such as class without coaching, behavioral coaching, emotion coaching, and emotion coaching based on emotional recognition system. The dependent variables were subjective evaluation, behavioral amplitude, and HRC (Heart Rhythm Coherence) of heart response. The results showed the highest positive evaluation, behavioral amplitude, and HRC at emotion coaching based on emotional recognition system. In post-doc analysis, the subjective evaluation showed no difference between emotion coaching and system based emotion coaching. However, the behavioral amplitude and HRC showed a significant response between two coaching situation. In conclusion, quantitative data such as behavioral amplitude and HRC was expected to solve the ambiguity of subjective evaluation. The emotion coaching of teacher using emotional recognition system was can be to improve positive emotion and psychological stability for children.

Mediating effect of self-control in the relationship between internet addiction and internet ethics awareness in healthcare-related college students (보건계열 대학생이 지각하는 인터넷 윤리의식과 인터넷 중독성향과 관계에서 자기 통제력의 매개효과)

  • Kang, Da Jeong;Jun, Soo Young
    • Journal of the Korean Data and Information Science Society
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    • v.28 no.2
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    • pp.261-270
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    • 2017
  • This research aims to investigate how healthcare professionals can control their behaviors in the internet and the status of their Internet addiction, thus emphasizing appropriate internet ethics awareness of students majoring in healthcare-related field, the future healthcare professionals. The goal of this research is to verify the mediating effect of self-control between the status of internet addiction of healthcare-related college students and their internet ethical awareness. A questionnaire survey was conducted on 236 students majoring in healthcare-related fields in 5 universities located in D city from March to April, 2016. The result of analyzing correlation of Internet addiction, internet ethics awareness, and self-control shows that self-control is negatively correlated with internet addiction but positively correlated with internet ethics awareness. The result of research suggests that self-control does have partial mediation effect on internet addiction and internet ethical awareness. Accordingly, it is necessary to develop mediator program that enhances self-control in order to improve internet ethical awareness of healthcare-related college students and develops measures to prevent addition to the internet, one of the primary cause of decline in internet ethics awareness.

Effects of FIN-TECH use motivation on User Attitude and Word Of Mouth Intention: Focus on a Innovation Resistance Tendency and Type of Message (Rational, Emotional) (핀테크 이용 동기에 따른 이용자 태도와 구전의도의 관계 - 혁신저항과 메시지 유형의 조절효과 -)

  • Seol, Sang-Cheol;Jung, Sung-Gwang;Choi, Woo-Young
    • Management & Information Systems Review
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    • v.36 no.5
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    • pp.195-222
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    • 2017
  • Today's economy is becoming more and more convergence between different industries as the demarcation of the boundaries of all areas is leading to innovations such as mobile and social network services. So Fin-tech is a new technology that can combine the advantages of mobile and Internet with the technology revolution to easily handle financial and IT tasks. This Fin-tech is a compound word of finance and technology. The purpose of this study is to investigate the overall structural relationship between Fin-Tech use motivation (usefulness, enjoyment) on user attitude and word of mouth intention. In addition, we investigated how FinTech use motivation, user attitude, and word of mouth intention change according to innovation resistance tendency. And, we examined how the motivation, user attitude, and word of mouth intention of FinTech change according to the message type (rational and emotional). The main results of this study are as follows. First, the usefulness and enjoyment of motivation for using FinTech have a positive effect on user attitude, and user attitude also has a positive effect on word of mouse intention. Second, the relationship between FinTech use motivation, user attitude, and word of mouth intention was found to difference according to consumers' innovation resistance. Third, it was found that the relationship between FinTech use motivation, user attitude, and word of mouth intention differs according to type of message (rational, emotional). At the conclusion of the study, the summary of the research results, implications and limitations, and future research direction are described.

Measurement of Nondestructive Residual Stress by Acoustoelasticity (음탄성에 의한 비파괴적 잔류응력 측정)

  • 박인근;이철구
    • Journal of Welding and Joining
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    • v.17 no.2
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    • pp.25-28
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    • 1999
  • 국내에 건설되어 거의 20∼30년 가동되고 있는 발전설비, 석유화학 플랜트 등 거대설비 기기의 건전성(integrity) 및 신뢰성 확보와 잔존수명 예측을 위해서는 구조물 내부 또는 표층부에 존재하는 결 함의 특성을 아는 것과 함께 그 재료의 특정 부위에 어느정도의 응력이나 변형이 있는가를 아는 것이 매우 중요하다. 일반적으로 강 용접부의 비파괴적 결함검출에는 주로 SV파(vertically shear wave)와 SH파(horizontally shear wave)라 불리는 횡파를 이용한 초음파사각탐상법이 실용화되어 이용되고 있다. 그러나 비파괴적인 방법에 의한 실험적인 잔류응력 측정, 변형해석법에는 전기 저항 및 자기 스트레인 게이지법, X선회절법, 광탄성법(photoelasticity), 모아레(Mohr's)법, 레이저스펙클(Laser speackle)법, 응 력도료법, Barkhausen Nosise법, Caustics법 등이 제시되어 있으나 그 유용성 면에서는 아직 해결되야할 문제가 많이 남아 있는 실정이다. 응력이나 변형을 해석하는 방법으로 이론적 방법, 계산적 방법 실험적 방법이 잇다. 이론적 방법에는 재료 역학적으로 취급하는 방법, 탄성론 등이 있고, 계산적인 방법에는 유한요소법이 있지만, 이론적 방법이나 계산적 방법만으로는 해석이 불가능한 경우가 많기 때문에 실험 적 방법이 필요하게 된다. 이 글에서는 파괴 시험 또는 다른 비파괴평가기술에 비해 간편한 측정, 높은 측정정도, 시험결과 도출의 신속성, 검사비용의 절감 등 많은 장점을 가지고 있고 실험적으로 유용성이 일부 검증되고 있는 음탄성법(Acoustoelasticity)에 의한 잔류응력 측정법에 관해 소개하고자 한다.TEX> mg/L(평균 49 mg/L)로 비교적 안정적인 처리효율을 보여주었다. 본 연구결과 HVC 공정은 화학약품 사용량의 절감 및 이에 따른 화학슬러지 발생량의 감소를 기대 할 수 있는 친환경기술로 유지관리비를 최소화할 수 있는 장점이 있었다. 않은 사람들 중 미래의 검진실행의지에 건강소식지가 영향을 미친 경우는 48.7%였다. 보건교육을 받은 후 유방암 자가검진 실천율은 사업군에서 53.9%로 받기 전의 27.3%보다 증가하였으나 대조군의 경우는 별 차이가 없었다. 연령별로는 60대가 가장 높았고 사업군에서 검진율의 증가분은 30대가 가장 컸다. 교육수준별로는 사업군은 고졸이, 대조군은 전문대졸이 가장 높았고 사업군에서 검진율의 증가분은 고졸에서 가장 컸다. 보건교육 후 유방암과 관련된 건강지식의 정도는 사업군이 3.7점으로 대조군보다 유의하게 높았으며, 유방암 자가검진법을 실천하는 사람들의 동기는 ‘일반 대중매체의 영향’이 가장 많았으며 건강소식지가 동기인 경우도 20.4%였다. 사업군에서 건강소식지가 유방암 자가검진법 실천에 영향을 미친 경우가 79.6%였으며 유방암 자가검진법에 관한 보건교육을 받고 실천하지 않은 사람들 중 미래의 실천의지에 건강소식지가 영향을 미친 경우는 43.6%였다. 이상의 소견에서 지역주민을 대상으로 인쇄매체를 통한 보건교육은 인쇄물만으로도 쉽게 실천 할 수 있는 유방암 자가검진법이 가장 효과적이었으며, 자궁암검진에 관해서도 검진을 받을 수 있도록 지역사회의 보건의료의 하부구조를 정비하여 제도적 장치를 마련하고 정보를 제공한다면 자궁암검진 실천율도 증가할 것이다.고 12.9% 의 발달율을 보여 유의적인 차이를 보이지 않았다. 이상의 결과로 보아 핵이식 수정란을 효율적으로 생산하기 위하여

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