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http://dx.doi.org/10.5352/JLS.2012.22.11.1552

Regulation of Pipernonaline on Biological Functions of Human Prostate Cancer Cells Based on Microarray Analysis  

Kim, Sang-Hun (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Kim, Kwang-Youn (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Yu, Sun-Nyoung (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Park, Seul-Ki (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Kwak, In-Seok (Department of Biological Sciences, Silla University Medicine)
Rhee, Moon-Soo (Korea Research Institute of Bioscience and Biotechnology)
Bang, Byung-Ho (Department of Food and Nutrition Science, College of Health Industry Eulji University)
Chun, Sung-Sik (Department of Food Science, International University of Korea)
Ahn, Soon-Cheol (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Publication Information
Journal of Life Science / v.22, no.11, 2012 , pp. 1552-1557 More about this Journal
Abstract
It has been reported that pipernonaline isolated from Piper longum Linn. has a wide biochemical and pharmacological effect, including antitumor activity in prostate cancer PC-3 cells. However, its mechanism and expression pattern of many genes involved in biological functions are not clearly understood. To perform the gene expression study in PC-3 cells treated with pipernonaline, a cDNA microarray chip composed of 44,000 human cDNA probes was used. As a result, cell cycle-related genes, apoptosis-related genes, and cell proliferation/growth-related genes have been identified in gene ontology of the DAVID database. These results suggest that pipernonaline has antitumor activity by regulating the expression pattern of genes involved in biological signaling pathway in prostate cancer PC-3 cells. Further, additional analysis of these microarray data can be a useful tool to identify the mechanism and discovery of novel genes in cancer therapy.
Keywords
Pipernonaline; PC-3 cells; microarray; expression; cell cycle; apoptosis; proliferation;
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