• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.19-23
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• 2000

The experiment was carried out to investigate the influence of light condition and polarity of explant on adventitious shoot formation from cotyledon of apple in vitro The treatment of light culture after 10days dark treatment showed effective result than other treatments on the rate of adventitious shoot formation on light intensity treatments and also the treatment of blue light treatment after 4days dark treatment showed more effective result than other treatments in light quality treatments. The polarity of explant influence on adventitious shoot formation. Adventitious multiple shoot formation occured at the proximal end of an excised cotyledon. In other words. shoot organogenesis occured at the proximal cut surfaces of both proximal and distal explants rather than at the distal cut surface of proximal explants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.171-176
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• 2008

Efficient plant regeneration system of birdsfoot trefoil (Lotus corniculatus L.) was development. The factors affecting the somatic embryo formation, its proliferation and regeneration capacity of leaf and stem explants of Empire cultivar was investigated. The highest number of somatic embryos was obtained on B5 medium supplemented with 1 mg/L NAA and 1 mg/L BA. Depending on different explants, highest frequency of embryogenic callus and regeneration were observed in Empire with leaf explants. The response from stem explants was slower and callus induction was less than that from leaf explants. Regenerated shoots formed complete plantlets in on 1/2 MS medium supplemented with 1 mg/L IBA. Regenerated plants were morphologically uniform with normal shape and growth pattern.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.15-18
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• 2002

Callus and shoot formation of leaf explants from in vitro propagated shoots and field grown plants depending on the position of leaf, and four boxthron cultivars were investigated. Callus formation of explants from both in vitro shoot and field grown plants as easily achieved at the cut surfaces of explants but the callus formed from leaf of in vitro shoots was hardened as the duration of culture was proceed. Calli were effectively induced from leaves detached from the middle position of both in vitro and in vitro plants on MS medium containing 0.5 mg/L NAA with 0.2 mg/L BA, and the growth of calli were better in field grown leaves than in vitro grown leaves. Shoot formation were effectively induced from leaves detached from the upper position in vitro plants, and the middle parts of in vitro plants on MS medium containing 0.01 mg/L (NAA with 0.2 mg/ BA. There was difference on the frequency of shoot formation among four different cultivars; 'Jindojaerae' was the best for shoot formation followed by 'Cheonyang', 'Younghagukija' and 'Cheongyangjaerae'.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.59-63
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• 1999

Efficient plant regeneration via shoot organogenesis from in vitro cultured leaf segments of chrysanthemum (Dendranthema grandiflora Tzvelev cv. Namjeon) was achieved. Adventitious shoot formation from leaf explants was greatly influenced by plant growth regulator, leaf age, light condition, explant number per culture vessel, and explant orientation. Leaf segments, obtained from fully expanded young 1-2nd leaves and inoculated 8 explants per petri-dish with adaxial surface contact with MS medium supplemented with 0.5 mg/L BA and 2.0 mg/L NAA, produced 100% regeneration frequency and 13.7 shoots per explant. Regenerated adventitious shoots were successfully rooted in MS medium with 0.1 mg/L NAA. The plantlets were acclimatized in artificial soil mixtures (Vermiculite:Perlite=1:1), and transferred to greenhouse for flowering. The regenerated plants showed normal phenotypes.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.479-484
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• 2000

This study was conducted to examine the effects of explant sources and plant growth regulators on mass propagation of Cyclamen persicum. Tuber, cotyledon, and petiole tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Shoots were not induced from calli on cotyledon and petiole explants cultured on MS medium containing various concentrations of 2,4-D or NAA. However, multiple shoots were formed directly from tuber explants cultured on the medium containing 0.5 and 1.0 mg/L 2,4-D or NAA. In MS medium with cytokinin alone, TDZ was more effective in shoot formation than BA or kinetin in all explants. The combinations of NAA and BA was found to be most effective in shoot formation from tuber, cotyledon and petiole explants. Especially, shoots were formed from all the tuber explants on the medium containing 0.5 mg/L of NAA and BA. Hormonal effects on root formation were examined by subculturing single shoots on MS medium containing NAA or IBA. The medium with 0.5 mg/L IBA was most effective in root induction and subsequent plantlet regeneration.

• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.329-333
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• 2001

Callus and shoot formation from medicinal crop, Lycium chinense Mill. cv. 'Cheongyang', as influenced by various media, explant sources and plant growth regulators were investigated. The rate of shoots formation, number of shoots, and fresh weight of shoots were the best on MS medium followed by B$_{5}$, WPH, and SH. Callus induction was more effective in leaf than internode segments, and was the best on MS medium containing 0.5 mg/L NAA with 0.2 mg/L BA. Effects of plant growth regulators in shoot formation were more effective in BA than TDA combined with NAA. Shoot formation from callus induced in leaf and internode segments was the best on MS medium containing 0.01 mg/L NAA with 0.2 mg/L BA.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.73-80
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• 2003

Useful Dianthus species were collected and selected from two native and seven foreign species distributed in Gangwon province. For in vitro breeding,. callus was induced from the explants of apical meristem, leaf, stem and the in vitro adventitious shoots on MS basal medium with 2.0 mg/L 2,4-D and 0.5 mg/L BA at 27$^{\circ}C$ under continuous light. After 3 weeks of culture, calli initiated the most highly from the leaf explants of D. chinensis Organogenic calli were able to be selected from the adventitious shoot-derived calli. For shoot regeneration, these organogenic calli were cultured on MS medium with the combination of 0.1 mg/L NAA＋1.0 mg/L BA under continuous light. Multiple shoots were proliferated with low frequency (about 30%) from those adventitious shootderived calli. Also, shoots initiated directly from the adventitious shoot explants without callus formation at high frequency of 52% when cultured on N6 medium containing 0.1 mg/L NAA and 1.0 mg/L BA in D. gratianopol. Multiple shoots and plantlets grew well and rooted on MS medium supplemented with 0.1 mg/L NAA. Regenerants with well-developed roots were transferred to 8-cm pots containing vermiculite at 85% relative humidity and 27$^{\circ}C$ These plantlets were acclimatized in artificial soil mixture and transferred to the greenhouse for flowering with normal phenotypes. M28 Mutant line was selected with white flowers from 0.03M EMS-treated organogenic calli derived from in vitro adventitious shoot explants of D. chinensis and set seeds.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.442-447
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• 2011

This study was carried out to assess the potential of hypocotyl explants for multiple-propagation by intensive sweetener variety with high yielding in stevia of self-fertilization. Agronomical character of self-fertilization shown a height range 72.0~120.7 cm, branch's 4.9~56.6 in stevia. Selection of high content of Rebaudioside A (RA) from segregating population in mixing variety have a variety, 'DA1' (55.2%) from HPLC analysis. On a developing of multiple-propagation in stevia, hypocotyl explants used to select for reasonable medium under the influence of each plant growth regulators concentrations. In a Mix treatment of plant growth regulators, as Murashige and Skoog medium with 1.5 mg/L BA and 0.5 mg/L Kinetin showed the most favorable. We got the multiple-propagation of 30 shoots from one inoculated hypocotyl explants as the result of these bases. Rooting induced superbly on MS medium with 0.1 mg/L IBA with inoculated shoots. All of the rooting plants were adapted to a normal seedling. This method of multi-propagation is used effectively in Rebaudioside A productivity elevation.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.347-351
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• 1998

Plant regeneration via organogenesis from leaf and stipule explants of micropropagated shoots of strawberry (Fragaria $\times$ ananassa cv. Suhong) was achieved. Leaf and stipule explants were detached from shoot-tip cultured shoots and cultured on MS medium with various combinations of BA and NAA under light or dark condition. Shoot regeneration from leaf explant was observed after 3 weeks in culture and was good at the high ratio of BA and NAA among various combination treatments. The highest shoot regeneration frequency from leaf explants was obtained with 1.0 mg/L BA and 0.1 mg/L NAA, in which 31.1% shoot regeneration frequency(1.7 shoots per leaf explant) was yielded. In case of stipule explants, shoot regeneration was largely affected by plant growth regulators during incubation under dark condition for initial 4 weeks but not under continuous light condition. The combination treatment with 2.0 mg/L BA and 0.1 mg/L NAA showed the most excellent shoot regeneration from stipule explants, where 44.4% regeneration frequency(4.0 shoots per explants) obtained. Regenerated shoots were rooted on MS medium with 0.1 mg/L NAA after shoot elongation, and the plantlets regenerated were transferred to soil mixtures with vermiculite and perlite for acclimation.

• Korean Journal of Plant Tissue Culture
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• v.21 no.3
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• pp.173-176
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• 1994

Stem and petiol explants of peony culture turned to brownish black soon after placing onto medium and degenerated to death. Disroloration was caused mainly by ferrous and calcium cloride. Nitrate was a main factor for the death of culture. The culture damage was increased with the increment of the medium salt strength. A few latent axillary buds were elongated to shoots without forming callus.