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Selection of Self-Fertilization Stevia and Development of Multiple Propagation Method using Hypocotyl Explants  

Seo, Seung Min (School of Applied Ecological Resources, Kyungpook National University)
Bae, Shin-Chul (Bio-crop development division, National Academy of Agricultural Science, Rural Development Administration)
Kim, Kyung-Min (School of Applied Ecological Resources, Kyungpook National University)
Publication Information
Korean Journal of Breeding Science / v.43, no.5, 2011 , pp. 442-447 More about this Journal
Abstract
This study was carried out to assess the potential of hypocotyl explants for multiple-propagation by intensive sweetener variety with high yielding in stevia of self-fertilization. Agronomical character of self-fertilization shown a height range 72.0~120.7 cm, branch's 4.9~56.6 in stevia. Selection of high content of Rebaudioside A (RA) from segregating population in mixing variety have a variety, 'DA1' (55.2%) from HPLC analysis. On a developing of multiple-propagation in stevia, hypocotyl explants used to select for reasonable medium under the influence of each plant growth regulators concentrations. In a Mix treatment of plant growth regulators, as Murashige and Skoog medium with 1.5 mg/L BA and 0.5 mg/L Kinetin showed the most favorable. We got the multiple-propagation of 30 shoots from one inoculated hypocotyl explants as the result of these bases. Rooting induced superbly on MS medium with 0.1 mg/L IBA with inoculated shoots. All of the rooting plants were adapted to a normal seedling. This method of multi-propagation is used effectively in Rebaudioside A productivity elevation.
Keywords
Hypocotyl; Multi-Propagation; Rebaudioside A; Stevia;
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