• Title/Summary/Keyword: 절단수준

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Quantitative Evaluation of Geotextile Void Structures Using Digital Image Analysis (디지털 이미지 분석을 이용한 지오텍스타일 공극 분포의 정량화)

  • Kim, Duhwan
    • Journal of the Korean Geosynthetics Society
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    • v.12 no.1
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    • pp.51-61
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    • 2013
  • This paper presents results from a study undertaken to quantitatively evaluate the geotextile pore sizes using optical image analysis. The evaluation was conducted by observing surfaces of coupons cut from resin-impregnated specimens of geotextile-geomembrane layered under various load conditions. Stereological concepts were applied to collect representative specimens from a series of laboratory tests. The sizes of voids enclosed by filaments were expressed in terms of the largest inscribing opening size (LIOS) distribution. The opening diameter corresponding to the 50% cumulative frequency decreased by about 45mm as the load increased from 10 to 300kPa and recovered to about 90% of its initial state on unloading back to 10kPa. The average void size was reduced by 32 and 16.5% as the geotextile was sheared against a textured geomembrane under normal stresses of 100 and 300kPa, respectively. The results showed how the LIOS distribution varied as a function of normal stress and interface shear displacement against a smooth and a textured geomembrane surfaces.

DNA Microarray Analysis of Gene Expression in Antifungal Bacterium of Bacillus lentimorbus WJ5 (DNA microarray를 이용한 항진균 활성세균 Bacillus lentimorbus WJ5의 유전자 발현 분석)

  • 이영근;김재성;장유신;조규성;장화형
    • Korean Journal of Microbiology
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    • v.39 no.3
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    • pp.141-147
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    • 2003
  • The simultaneous expression levels of antifungal activity related genes was analyzed by DNA microarray. We constructed DNA chips contained 2,000 randomly digested genome spots of the antifungal bacterium of Bacillus lentimorbus WJ5 and compared its quantitative aspect with 7 antifungal activity deficient mutants induced by gamma radiation ($^{60}Co$). From the analysis of microarray hybridization by the Gene Cluster (Michael Eisen, Stanford Univ.), totally 408 genes were expressed and 20 genes among them were significantly suppressed in mutants. pbuX (xanthine permease, K222), ywbA (phosphotransferase system enzyme II, K393), ptsG (PTS glucose specific enzyme II ABC component, K877), yufO (ABC transporter (ATP-binding protein), K130l), and ftsY (signal recognition particle (docking protein), K868) were simultaneously down-regulated in all mutants. It suggested that they were supposed to be related to the antifungal activity of B. lentimorbus WJ5.

The effect of cavity configuration on the mechanical properties of resin composites (중합환경에 따른 복합레진의 물리적 성질에 관한 연구)

  • Ryu, Gil-Joo;Park, Sang-Jin;Choi, Kyoung-Kyu
    • Restorative Dentistry and Endodontics
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    • v.27 no.3
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    • pp.239-248
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    • 2002
  • 이 연구의 목적은 와동의 형태가 굴곡강도와 탄성계수 등 복합레진의 물리적 성질에 미치는 영향을 평가하는 것이다. 복합레진은 Clearfil$^{TM}$ AP-X(Kuraray, Japan)와 Esthet-X$^{TM}$(Dentsply, USA)가 이용되었으며, 상아질 접착제는 Clearfil$^{TM}$ SE Bond(Kuraray, Japan)와 Prime & Bond NT$^{TM}$(Dentsply, USA)를 사용하였다. 대조군의 시편은 split steel mold(25mm$\times$2mm$\times$2mm) 내에 상기 2종류의 복합레진을 충전하여 2개의 대조군 시편을 제작하였으며, 2.4 및 3.4의 C-factor를 부여하기 위한 유리 모형와동을 제작하고, 와동 내에 상기 2종류의 복합레진을 충전하기 전 유리와동의 내면은 sandblasting 처리하고 각각의 복합레진과 동일회사 제품의 상기 상아질 접착제로 처리한 후, 복합 레진을 각각 충전하여 4개의 실험군을 제작하였다. 제작된 실험군 시편은 저속 diamond saw로 충전된 복합레진 부위의 중심부를 통과하도록 절단하여 레진기둥(25mm$\times$2mm$\times$2mm)이 되도록 제작하였다. 제작된 시편을 37$^{\circ}C$의 증류수에 24시간 동안 보관 후, 만능시험기(EZ Test, Shimadzu, Japan)를 이용하여 분당 1mm의 crosshead speed로 3점 굴곡강도를 측정하였다. 또 Linometer(R&B, Korea)를 이용하여 복합레진의 중합수축량을 측정하였으며 굴곡강도측정 후 시편의 파단면은 주사전자현미경(S-2300, Hitachi, Japan)을 이용하여 관찰하였다. 실험결과의 통계분석은 95% 수준의 one-way ANOVA/Tukey's test를 이용하여 결과를 얻었다. 실험에 이용된 2종류 복합레진의 굴곡강도와 탄성계수는 C-factor치 증가에 따라 감소하였으며, 파단면 또한 C-factor의 증가에 따라 더 불규칙해지는 양상을 나타내었다. 본 실험의 결과 hybrid형 복합레진이 micro-hybrid형 복합레진에 비해 C-factor의 영향을 더 많이 받는 것으로 나타났으며, 와동의 C-factor증가가 굴곡강도나 탄성계수와 같은 복합레진의 물리적 성질을 저하시킨다는 것을 의미하였다.

Production and Characterization of a Monoclonal Antibody against Surface Glycoprotein, gp6 1, on K562 Erythroleukemia Cells (K562 적혈구암 세포주의 표면 당단백질에 대한 단클론항체의 생성 및 특성)

  • 김한도;정재훈;홍선화;김정락;한규형;임운기;유미애;이경희;강호성
    • The Korean Journal of Zoology
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    • v.39 no.1
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    • pp.12-20
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    • 1996
  • A multipotential hematopojetic cell line, 1(562 cell, was differentiated into megakaryocyte by a chemical inducer, PMA, with an enhanced expression of gpIlla accompaning with a distinct morphological change. On the other hand, 1(562 cells were differentiated into erythrocytes by other chemical inducers, DMSO or butyrate, with a concomitant increase in hemoglobin accumulation. An antigen of apparent molecular weight of 61 kDa was identified on the surface of 1(562 cells by using monoclonal antibody raised against 1(562 cells. The antigen was considered to be a glycoprotein molecule rich in sialic acids and the epitope of antigen was sensitive to neuraminidase digestion or peroxidase oxidation, but resistant to heat treatment. The 61 kDa surface antigen was increased or decreased in its expression along differentiation of 1(562 cells into megakaryocytes or erythrocytes, respedively.

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Quality of Wet Noodle Prepared with Wheat Flour and Mushroom Powder (버섯분말을 첨가한 생면의 품질특성)

  • Kim, Young-Soo
    • Korean Journal of Food Science and Technology
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    • v.30 no.6
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    • pp.1373-1380
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    • 1998
  • Wet noodles were prepared with wheat flour and mushroom powder (oyster and oak mushrooms), and effects of added mushroom powders on dough rheology and wet noodle quality were examined. Particle size distribution of mushroom powder ranged from 0.04 to $500\;{\mu}m$, which was different from that of wheat flour. The initial pasting temperature in amylograph, and the water absorption and the dough development time in farinograph increased with the increase of mushroom powder. The peak and final viscosities in amylograph, and dough stability in farinograph decreased with the increase of mushroom powder. Decrease of L value and increase of a and b values were shown with the increase of mushroom powder in wheat flour-mushroom powder composite as well as wet noodles. The cooked weight and volume of cooked noodles were decreased, but the turbidity of soup were increased with the addition of mushroom powder. Most of texture parameters (hardness, cohesiveness, chewiness and cutting force) of cooked noodles decreased with the addition of oyster mushroom powder, but increased with the addition of oak mushroom powder. From the result of sensory evaluation, wet noodles containing 3% oyster mushroom powder and 5% oak mushroom powder were rated as high quality wet noodles.

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The Effects of Socioeconomic Position and Health Behavior on Geriatric Depressive Symptom (노인우울증에 대한 사회경제적 지위요인과 건강행태요인에 관한 연구)

  • Lee, Hyun-Joo;Kahng, Sang-Kyoung;Lee, Jun-Young
    • 한국노년학
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    • v.28 no.4
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    • pp.1129-1145
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    • 2008
  • In order to develop practical implications for depression among the elderly, this study examines socioeconomic and health-behavioral risk factors of geriatric depression diagnosis. Sample consists of 964 elders aged 65 and over, living in an urban community. Using the Short-form Geriatric Depression Scale(SGDS), study subjects were screened for geriatric depression to those who presented 10 points and over in SGDS. Descriptive statistics shows that about 22.2% elders are estimated to suffer from geriatric depression. Predictors of geriatric depression diagnosis were examined through logistic regression. Results show that elders who live with family, who have medical insurance, who have higher education, who don't smoke, and who do more physical activities were less likely to suffer from geriatric depression. These findings indicate that socioeconomic position such as health insurance, education and health behaviors such as smoking, physical activities are closely related to geriatric depression, suggesting that interventions on socioeconomic position and health behaviors may be effective to reduce depression among the elderly.

Efficiency of PDNR (Polydeoxyribonucleotide) extraction from various plant species and its in vitro wound healing activity (다양한 식물에서의 PDRN(Polydeoxyribonucleotide) 추출 수율 비교 및 상처치유 효능 분석)

  • Song, Mi-Hee;Choi, Moon-Hyeok;Jeong, Jin-Hyoung;Lee, Sang-Sik;Jeong, Woo-Young
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.15 no.5
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    • pp.387-395
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    • 2022
  • PDRN (Polydeoxyribonucleotide) is a DNA-derived polymer that promotes self-renewal of damaged cells and tissues as a tissue regeneration active material. PDRN is a DNA fragment cut into small sizes by various physical or chemical methods. When administered to the body, PDRN binds and stimulates the adenosine A2A receptor on the surface of tissue cells to promote cell regeneration, accelerate wound healing, and reduce pain. Although PDRN is prepared from testis or semen of fish in most cass, PDRN extraction from various plants species was performed in the present study. Among 7 tested plant species, the highest DNA yield and purity was obtained form mugwort (Chrysanthemum coronarium, C.c), followed by broccoli (Brassica oleracea, B.o). Then, we evaluated the in vitro wound healing capacity of PDRNs prepared from these two selected plants. PDRN from C.c and B.o. significantly stimulated the wound healing process at ㎍/ml range. The present study suggests that PDRN from plant species can be an effective alternative to PDRN from marine organism.

Changes in Quality Characteristics of Peony Major Varieties on Harvest Stage (수확 단계에 따른 작약 주요 품종의 수확후 품질 특성 변화)

  • Haejo Yang;Ji Hyun Lee;Sooyeon Lim;Ji-Weon Choi
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.60-60
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    • 2020
  • 본 연구는 수확 단계에 따른 작약 주요 품종의 수확후 품질 특성을 조사하여 품종별 적정 수확 시기를 구명하고자 수행하였다. 절화 작약은 '두체스', '레드참', '보울오브크림' 품종을 사용하였으며, 세 품종 모두 1, 2, 3, 4단계로 구분 지어 수확하여 실험에 사용하였다. 수확한 작약은 40cm로 재절단하여 chrysal professional III 용액이 담긴 화병에 꽃아 생체중, 증산량, 수분흡수량, 개화 단계 변화 등을 매일 조사하였고, 이를 통해 수분균형 및 절화 수명 등의 결과를 도출하였다. '두체스' 품종의 품질 특성 변화를 조사한 결과, 1단계에 수확한 절화의 만개소요일수는 5.5일, 만개유지일수는 2.9일, 총 절화수명은 8.4일로 다른 수확단계보다 1~3일 더 긴 것으로 조사되었으며, 생체중증가율 및 화경 변화 결과를 통해 1단계 수확 시에도 개화는 정상적으로 진행되는 것을 확인할 수 있었다. '레드참' 품종의 품질 특성 변화 조사 결과, 1단계에 수확한 절화의 만개소요일수는 7.9일, 만개유지일수는 3.3일, 총 절화수명은 11.2일로 다른 수확단계보다 1~4일 더 긴 것으로 조사되었다. '보울오브크림' 품종의 품질 특성 변화 조사 결과, 1단계 수확한 절화는 30% 수준만 만개하여 조기 수확에 따른 문제점이 확인되었지만, 2단계 수확한 절화는 개화와 만개 모두 100% 진행되었으며, 만개소요일수는 6.4일, 만개유지일수는 4.5일, 총 절화수명은 10.9일로 다른 수확단계보다 1~2일 긴 것으로 조사되었다. 본 연구 결과, '두체스' 와 '레드참' 품종의 적정 수확단계는 1단계, '보울오브크림' 품종의 적정 수확단계는 2단계가 적절할 것으로 판단된다.

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Comparative analysis of status of safety accidents and importance-performance analysis (IPA) about precautions of safety accidents by employment type of industry foodservices in Jeonbuk area (전북지역 산업체급식소 조리종사자의 고용형태에 따른 안전사고 실태 및 안전사고 예방관리에 대한 중요도와 수행도 분석)

  • So, Hee;Rho, Jeong Ok
    • Journal of Nutrition and Health
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    • v.50 no.4
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    • pp.402-414
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    • 2017
  • Purpose: The purpose of the study was to evaluate the status of safety accidents and importance-performance analysis (IPA) between regular and non-regular employees in industry foodservices. Methods: The participants were regular employees (n = 119) and non-regular employees (n = 163) in industry foodservices in the Jeonbuk area. Demographic characteristics, status of safety accidents, safety education, and importance and performance status were assessed using a self-administered questionnaire. Results: Approximately 66.4% of regular employees and 53.4% of non-regular employees experienced safety accidents (p < 0.05). Types of safety accidents of regular and non-regular employees were mostly burns, and causes were mostly from their own negligence. Approximately 98.3% of regular employees and 95.1% of non-regular employees experienced safety education. Approximately 88.9% of regular employees and 96.8% of non-regular employees received safety education from dietitians. Approximately 41.9% of regular employees and 50.0% of non-regular employees had difficulty applying the contents of safety education due to lack of time during work. As a result of IPA, regular and non-regular employees were aware of the importance of the following and performed them well: 'Clean the floor of the work place', 'Arrange in the work area', 'Wear safety shoes', 'Check for heater cord', and 'Safety cooking when using oil'. On the other hand, they were not aware of the importance of the following and performed them insufficiently: 'Check for the MSDS', 'Aware of chemical signs', 'Wear protection gloves etc.', 'Do stretching exercise', and 'Using ancillary tools'. Conclusion: Therefore, it is necessary to improve the consciousness of dietitians for effective application of safety education contents, development of contents, especially MSDS, and related things.

Effects of Gamma Irradiation on Color Changes and Antioxidative Activities of Caesalpinia sappan L. (감마선 조사에 의한 소목추출물의 색상변화 및 항산화 활성 평가)

  • Kwon, Hyung-Jun;Jung, U-Hee;Park, Hae-Ran;Shin, Dong-Hwa;Jo, Sung-Kee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.8
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    • pp.1055-1061
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    • 2007
  • Caesalpinia sappan L. is an indeciduous tree distributed in China, India, Miyanmar and Vietnam. Its heartwood has long been used in oriental folk medicines to treat diseases. In this study, antioxidative activities of Caesalpinia sappan L. and the effect of gamma irradiation on its chemical and biological properties were investigated. The ethyl acetate fraction (EtOAc fr.) of Caesalpinia sappan L. was irradiated with 100 kGy of gamma ray. The dark red color of EtOAc fr. was significantly (p<0.05) removed by irradiation (Hunter L and b values increased and a value decreased). The total phenolic content of EtOAc fr. was 865 mg/g and it was increased to 1195 mg/g by gamma irradiation. DPPH radical and superoxide anion radical scavenging activities and lipid peroxidation inhibitory activity of EtOAc fr. were very high and its activities were also increased by gamma irradiation. EtOAc fr. also inhibited the irradiation-induced DNA damage of lymphocyte as determined by comet assay. In conclusion, EtOAc fr. of Caesalpinia sappan L. extract showed high antioxidative activities in vitro. Furthermore, gamma irradiation on EtOAc fr. ameliorated the color and antioxidative properties. Therefore, it can be suggested that Caesalpinia sappan L. may be a good material for antioxidant function and gamma irradiation may be applied for the improvement of chemical and biological properties of Caesalpinia sappan L.