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http://dx.doi.org/10.17661/jkiiect.2022.15.5.387

Efficiency of PDNR (Polydeoxyribonucleotide) extraction from various plant species and its in vitro wound healing activity  

Song, Mi-Hee (Hyundaemedtech CO.Ltd)
Choi, Moon-Hyeok (Department of Electronic and Communication Engineering)
Jeong, Jin-Hyoung (Department of Biomedical IT)
Lee, Sang-Sik (Department of Biomedical Engineering, Catholic Kwandog University)
Jeong, Woo-Young (Department of Biomedical Science, Catholic Kwandog University)
Publication Information
The Journal of Korea Institute of Information, Electronics, and Communication Technology / v.15, no.5, 2022 , pp. 387-395 More about this Journal
Abstract
PDRN (Polydeoxyribonucleotide) is a DNA-derived polymer that promotes self-renewal of damaged cells and tissues as a tissue regeneration active material. PDRN is a DNA fragment cut into small sizes by various physical or chemical methods. When administered to the body, PDRN binds and stimulates the adenosine A2A receptor on the surface of tissue cells to promote cell regeneration, accelerate wound healing, and reduce pain. Although PDRN is prepared from testis or semen of fish in most cass, PDRN extraction from various plants species was performed in the present study. Among 7 tested plant species, the highest DNA yield and purity was obtained form mugwort (Chrysanthemum coronarium, C.c), followed by broccoli (Brassica oleracea, B.o). Then, we evaluated the in vitro wound healing capacity of PDRNs prepared from these two selected plants. PDRN from C.c and B.o. significantly stimulated the wound healing process at ㎍/ml range. The present study suggests that PDRN from plant species can be an effective alternative to PDRN from marine organism.
Keywords
Cell Migration; Hepatic stellate cell; Mesenchymal stem cell; ONGHEPA1; PDRN; Woundhealig assay;
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