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DNA Microarray Analysis of Gene Expression in Antifungal Bacterium of Bacillus lentimorbus WJ5  

이영근 (한국원자력연구소 방사선이용연구부)
김재성 (한국원자력연구소 방사선이용연구부)
장유신 (한국원자력연구소 방사선이용연구부)
조규성 (한국원자력연구소 방사선이용연구부)
장화형 (한국원자력연구소 방사선이용연구부)
Publication Information
Korean Journal of Microbiology / v.39, no.3, 2003 , pp. 141-147 More about this Journal
Abstract
The simultaneous expression levels of antifungal activity related genes was analyzed by DNA microarray. We constructed DNA chips contained 2,000 randomly digested genome spots of the antifungal bacterium of Bacillus lentimorbus WJ5 and compared its quantitative aspect with 7 antifungal activity deficient mutants induced by gamma radiation ($^{60}Co$). From the analysis of microarray hybridization by the Gene Cluster (Michael Eisen, Stanford Univ.), totally 408 genes were expressed and 20 genes among them were significantly suppressed in mutants. pbuX (xanthine permease, K222), ywbA (phosphotransferase system enzyme II, K393), ptsG (PTS glucose specific enzyme II ABC component, K877), yufO (ABC transporter (ATP-binding protein), K130l), and ftsY (signal recognition particle (docking protein), K868) were simultaneously down-regulated in all mutants. It suggested that they were supposed to be related to the antifungal activity of B. lentimorbus WJ5.
Keywords
antifungal activity; Bacillus lentimorbus WJ5; DNA microarray; gamma radiation; mutant;
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