• Journal of Bio-Environment Control
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• v.11 no.3
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• pp.139-143
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• 2002

Cotton Glutathione S-Transferase (GST: EC 2.5.1.18) was cloned and overexpressed in tobacco (Nicotiana tabacum) plants. Northern blot analysis confirmed the successful transformation of cotton gst gene in tobacco plant. Type I and Type ll transcript patterns were identified in transgenic tobacco plants and only Type I transcripts were discussed in this paper, The activity of GST in the type II transgenic plants was about 1.5-fold higher than those of the wild type and non-expresser by using 1-chloro-2,4-dinitrobenzene (CDNB) and reduced glutathione as the substrate. The expression of cotton GST in tobacco plants proved that Gh-5 could be translated into functional protein. Type II transgenic plants produced functional GST in the cells. The effects of cotton GST in the seedlings was evaluated by growing the control and transgenic seedlings at $15^{\circ}C$ in the growth chamber in the light. Overexpressors were grown well compared to the control plants (non-expressors). lo test far tolerance to salinity, seeds of Gh-5 overexpressors and the wild type Xanthi seedlings were grown at 0, 50, 100, 150, and 200 mM NaCl solution. Gh-5 transgenic seedlings showed higher growth rate over control seedlings on 50 and 100 mM NaCl solution. There was no difference in growth rate at 150 and 200mM NaCl concentration.

• Korean Journal of Environment and Ecology
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• v.26 no.4
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• pp.576-587
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• 2012

As being actual physiognomical vegetation on Daegwang sand dune in Imjado, the widest area is occupied by Pinus thunbergii community planted as windbreak forest whereas those communities such as Robinia pseudoacacia community, Elymus mollis community, Imperata cylindrica var. koenigii - Elymus mollis community, Carex kobomugi community, Ischaemum antephoroides community, Imperata cylindrica var. koenigii community, Phragmites communis community, Imperata cylindrica var. koenigii - Calamagrostis epigeios community occupy as band shape or patch. According to the result of the data collected and analyzed based on phytosociological method regarding 74 plots of survey area, the species composition of Daegwang sand dune vegetation is classified total 10 vegetation units comprises 7 communities and 5 groups. The 7 communities are classified into Pinus thunbergii community, Robinia pseudoacacia community, Rosa rugosa var. rugosa community, Ischaemum antephoroides community, Carex kobomugi community, Calamagrostis epigeios community, and the sub-units of Pinus thunbergii communities are classified into 3 groups of Pteridium aquilinum var. latiusculum group, Elymus mollis group, Pinus thunbergii topical group and the sub-units of Calamagrostis epigeios communities are classified into 2 groups of Phragmites communis group, Calamagrostis epigeios tipical group. The zonation of vegetation from coastal line indicated with the order of Elymus mollis - Carex kobomugi, Carex pumila, Lathyrus japonicus - Calystegia soldanella - Vitex rotundifolia, Lathyrus japonicus, Ischaemum antephoroides - Rosa rugosa var. rugosa. According to the analysis result of longitudinal section, it was found to be those types with wide width herbaceous vegetation of foredune, smooth slope of foredune, lots of dune ridges with no structure were less vulnerable to erosion of sand dune and advantageous to its recovery.

• Korean Journal of Environmental Biology
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• v.35 no.3
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• pp.380-388
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• 2017

Factors affecting spatial distribution of halophytes were analyzed in June 2012 at the Goraebul coastal dunes. In the Goraebul sand dune, distribution of halophytes was divided into three groups. The first group belonging to Elymus mollis, Carex kobomugi, Calystegia soldanella, Ixeris repens and Glehnia littoralis was distributed in the ridge of primary sand dune and dune slack. The second group belonging to Lathyrus japonicus and Zoysia macrostachya was distributed in the dune slack. The third group belonging to Pinus thunbergii, Vitex rotundifolia and Linaria japonicus was distributed in the pine forest of the secondary sand dune. E. mollis, C. kobomugi, C. soldanella, I. repens and G. littoralis was distributed in relatively unstable habitat of sand dunes due to the large amount of sand movement. V. rotundifolia was distributed in a relatively stable habitat. Factors that have the greatest influence on distribution of halophytes in the Goraebul sand dunes are distance from the seashore, topography, and the pine forest. The Goraebul sand dune is a relatively well-preserved area with minimal human intervention. Therefore, different distribution of physico-chemical factors by natural processes is essential to spatial distribution of halophytes than other sand dunes in Korea. Significant natural processes in the Goraebul sand dunes were advance and retreat of coastlines from waves, erosion and sedimentation of sand due to wind and waves, and dispersal of seawater.

• Korean Journal of Poultry Science
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• v.43 no.1
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• pp.47-54
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• 2016

The aim of this study was to investigate the expression patterns of key genes involved in lipid metabolism in response to dietary Coenzyme Q10 (CoQ10) in hens. A total of 36 forty week-old Lohmann Brown were randomly allocated into 3 groups consisting of 4 replicates of 3 birds. Laying hens were subjected to one of following treatments: Control (BD, basal diet), T1 (BD+ CoQ10 100 mg/kg diet) and T2 (BD+ micellar of CoQ10 100 mg/kg diet). Birds were fed ad libitum a basal diet or the basal diet supplemented with CoQ10 for 5 weeks. Total RNA was extracted from the liver for quantitative RT-PCR. The mRNA levels of HMG-CoA reductase(HMGCR) and sterol regulatory element-binding proteins(SREBP)2 were decreased more than 30~50% in the liver of birds fed a basal diet supplemented with CoQ10 (p<0.05). These findings suggest that dietary CoQ10 can reduce cholesterol levels by the suppression of the hepatic HMGCR and SREBP2 genes. The gene expressions of liver X receptor (LXR) and SREBP1 were down regulated due to the addition of CoQ10 to the feed (p<0.05). The homeostasis of cholesterol can be regulated by LXR and SREBP1 in cholesterol-low-conditions. The supplement of CoQ10 caused a decreased expression of lipid metabolism-related genes including $PPAR{\gamma}$, XBP1, FASN, and GLUTs in the liver of birds (p<0.05). These data suggest that CoQ10 might be used as a dietary supplement to reduce cholesterol levels and to regulate lipid homeostasis in laying hens.

• Journal of Aquaculture
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• v.9 no.2
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• pp.151-157
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• 1996

In order to study the effects of dietary E. compresa on the growth and blood properties in young Israeli strain of common carp (mean. 8 g BW.). Fish were fed the commercial diet (control) and three experimental diets, $1\%,\;5\%\;and\;10\%$ of E. compressa powder were added to the control diet. After four months of feeding trial fish were bled by cardiac puncture and some hematological parameters were analyzed as physiological indices. 1) Following supplementation, positive responses in plasma albumin and glucose levels, and decreased body weight gain, feed efficiency and hematocrit. 2) Condition factor (fatness) in creased up to $50\%$, but decreased with $10\%$ supplementation. 3) Hb, GOT and GPT did not show any remarkable difference among all diet groups.

• Journal of Life Science
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• v.17 no.8 s.88
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• pp.1172-1175
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• 2007

We observed that exogenously applied ethanol changed the activities of aldehyde oxidases (AO) in the primary roots of maize (Zea mays). The stimulatory effect of ethanol on the aldehyde oxidase activities was concentration dependent; the AO activities were slightly weaker with 0.2 - 0.4% ethanol and stronger with 0.8 - 1.0% ethanol than the level of control. The promotion of AO activities was not explained by the increased transcription of two AO genes in maize. In contrast, ethanol strongly increased the amount of AO proteins, indicating that ethanol enhanced AO activities by promoting the translation. Among three alcohols including ethanol, methanol and isopropanol, only ethanol promoted AO activities. These results suggested that enhancement of AO activities was specific to ethanol, whose level could be naturally increased when the plant roots drove fermentation to overcome low oxygen stresses.

• Journal of the Korean Wood Science and Technology
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• v.35 no.4
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• pp.52-60
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• 2007

Plant can detoxify the effect of the volatile organic compounds (VOC) such as formaldehyde and toluene, however, mechanisms of VOC detoxification are largely unknown in plant system. This study was performed to investigate phenotypic changes of Arabidopsis seedlings upon treatment of either formalin or toluene. Formalin treatment up to twenty four hours didn't cause any significant phenotypic damages on the leaf surface of 27 DAG Arabidopsis seedlings. However, the protein profile of formalin-treated seedlings was significantly different from that of mock control. Using automated electrophoresis system, the molecular weight of each formaldehyde-responsive protein (FRP) was predicted and its formaldehyde-dependent expression was confirmed at transcription level by quantitative real-time RT-PCR analysis. Four FRPs isolated in this study are the novel proteins with unknown functions but highly homologous to the stress-related proteins.

• The Journal of the Convergence on Culture Technology
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• v.3 no.4
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• pp.145-152
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• 2017

The accident rate is higher in the small workplace than the large workplace. It is desirable to focus on the small workplace for the disaster prevention activities. The disaster prevention activities for the small workplace are achieved through the private consignment agencies' technical guidance. but it has exposed many demand of the private consignment agencies' experts and its effectiveness has been strengthened. The purpose of this study, it is to provide the system improvement plan that the problem can be solved and to present a plan to improve the effectiveness of the proposed system. It is to listen to the opinions of experts and the direction of the study was set up.

• Journal of Powder Materials
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• v.26 no.1
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• pp.11-15
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• 2019

In this study, a method to remove residual powder on a multi-layered graphene and a new approach to transfer multi-layered graphene at once are studied. A graphene one-step transfer (GOST) method is conducted to minimize the residual powder comparison with a layer-by-layer transfer. Furthermore, a residual powder removing process is investigated to remove residual powder at the top of a multi-layered graphene. After residual powder is removed, the sheet resistance of graphene is decreased from 393 to 340 Ohm/sq in a four-layered graphene. In addition, transmittance slightly increases after residual powder is removed from the top of the multi-layered graphene. Optical and atomic-force microscopy images are used to analyze the graphene surface, and the Ra value is reduced from 5.2 to 3.7 nm following residual powder removal. Therefore, GOST and residual powder removal resolve the limited application of graphene electrodes due to residual powder.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.4
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• pp.624-629
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• 2014

Obesity is the leading metabolic disease in industrialized countries and is closely associated with coronary heart disease, hypertension, diabetes, and cancer. The objective of this study was to evaluate the anti-adipogenic effects of two roasted coffee beans, Vietnam robusta (VR) and Ethiopia Mocha Sidamo G2 (ES), as well as fermented coffee beans with Monascus ruber (MR) mycelium on differentiation of 3T3-L1 preadipocytes. Treatments with 1,000 ${\mu}g/mL$ of hot water extract from coffee beans significantly reduced intracellular lipid accumulation. In addition, VR more effectively inhibited transcription factors such as $PPAR{\gamma}$, $C/EBP{\alpha}$, FAS, and aP2 compared to ES. Further, ES fermented with MR showed more effective anti-adipogenic activity than non-fermented ES. These results suggest that VR and ES inhibit adipocyte differentiation which may contribute to their anti-adipogenic properties.