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Increase of Salt and Low Temperature Tolerance by Overexpressing Glutathione S-Transferase (GST) Gene  

Jun Chol Kim (Department of Biology, College of Natural Sciences, Kangwon National University)
Il Seop Kim (Division of Applied Plant Science, College of Agriculture and Life Sciences, kangwon National University)
Won Hee Kang (Division of Applied Plant Science, College of Agriculture and Life Sciences, kangwon National University)
Publication Information
Journal of Bio-Environment Control / v.11, no.3, 2002 , pp. 139-143 More about this Journal
Abstract
Cotton Glutathione S-Transferase (GST: EC 2.5.1.18) was cloned and overexpressed in tobacco (Nicotiana tabacum) plants. Northern blot analysis confirmed the successful transformation of cotton gst gene in tobacco plant. Type I and Type ll transcript patterns were identified in transgenic tobacco plants and only Type I transcripts were discussed in this paper, The activity of GST in the type II transgenic plants was about 1.5-fold higher than those of the wild type and non-expresser by using 1-chloro-2,4-dinitrobenzene (CDNB) and reduced glutathione as the substrate. The expression of cotton GST in tobacco plants proved that Gh-5 could be translated into functional protein. Type II transgenic plants produced functional GST in the cells. The effects of cotton GST in the seedlings was evaluated by growing the control and transgenic seedlings at $15^{\circ}C$ in the growth chamber in the light. Overexpressors were grown well compared to the control plants (non-expressors). lo test far tolerance to salinity, seeds of Gh-5 overexpressors and the wild type Xanthi seedlings were grown at 0, 50, 100, 150, and 200 mM NaCl solution. Gh-5 transgenic seedlings showed higher growth rate over control seedlings on 50 and 100 mM NaCl solution. There was no difference in growth rate at 150 and 200mM NaCl concentration.
Keywords
Glutathione S-transferases (GST); low temperature; overexpession; salinity;
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