• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.3
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• pp.243-252
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• 2020

Using Fervidobacterium islandicum AW-1, polar low molecular weight keratin peptides were produced and confirmed through factors related to the skin elasticity. As a result of confirming the cytotoxicity and collagen synthesis ability according to the concentration of the polar low molecular weight keratin peptide in human fibroblasts, it was confirmed that the cytotoxicity did not appear and the collagen synthesis in human fibroblasts was increased. A mask pack containing a polar low-molecular weight keratin peptide was used, and a test product was used for 4 weeks in 22 healthy women subjects. As a result, it showed statistically significant effects on skin elasticity, skin torsion elasticity, skin color and moisture improvement. Through this test, it was confirmed that the polar low-molecular keratin peptide can be used as a cosmetic ingredient that helps improve skin elasticity.

• Korean Journal of Food Science and Technology
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• v.53 no.6
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• pp.695-700
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• 2021

The purpose of this study was to prepare low-molecular weight collagen using a commercial proteolytic enzyme (Protamex) from collagen extracted from feet of Korean native chicken and to investigate the quality characteristics of this collagen. The collagen content of Korean native chicken feet was 13.9 g/100 g, which was higher than the 6.21 g/100 g of general broilers. It was found that the content of low molecular weight collagen increased as the concentration of proteolytic enzymes and reaction time increased. In particular, reaction with 1% Protamex for 7 h resulted in 55.6% of low molecular weight (1,000-5,000 Da) collagen content, and the average molecular weight was 5,390 Da. Regarding the texture of the enzyme-treated collagen, the collagen with high molecular weight peptides decomposed into low molecular weight peptides, and the gel type could not be formed, whereas the sol type was maintained.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.10a
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• pp.133-134
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• 2001

발효식품에 함유되어 있는 저분자 peptide는 발효 동안이나 가수분해되는 과정에서 생산된다. 이렇게 생성된 저분자 peptide는 생물학적 활성이 크다고 보고되고 있다. 생리기능성 펩티드의 종류를 살펴보면, 동물성 유래의 호르몬류나 식물성 유래의 효소 저해물질 등 활성형으로 존재하는 현재적(顯注的) 생리 활성 peptide, 소화과정이나 식품가공 과정에서 부분 가수 분해에 의해 불활성형 단백질로부터 파생되는 잠재적 생리활성 peptide, cyclo (His-Pro) 등과 같은 가수분해 이외의 기구에 의해 생성되는 생리활성 펩타이드 등이 있다. (중략)

• Journal of the Korean Applied Science and Technology
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• v.36 no.3
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• pp.1018-1027
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• 2019

Natural-derived protein-derived low molecular weight peptides have been known to have physiological activities such as antioxidant, hypertension relief, immunomodulation, pain relief and antimicrobial activity. In this study, the low-molecular peptides were produced using commercial proteases (alcalase, bromelain, flavourzyme, neutrase, papain, protamex), and the antioxidant activity (DPPH scavenging activity, superoxide radical scavenging activity, hydroxy radical scavenging activity, and metals chelation capacity), constituent amino acid and molecular weight of the peptide were analyzed. Enzyme reaction was performed by adding 50 g of chopped Ogae meat slurry and 2%(w/v) protein enzyme into the enzyme reactor for 2 h at a pH of 6 and a temperature of $60^{\circ}C$. The degree of hydrolysis(%) after the reaction ranged from $36.65{\pm}4.10%$ to $70.75{\pm}5.29%$. The highest degree of hydrolysis of protamex was 46.3%, and the highest value of papain hydrolysate was $70.75{\pm}5.29%$. On the other hand, alcalase hydrolysate showed the lowest value of $36.65{\pm}4.10%$. Bromelain-treated low molecular weight peptides showed the highest DPPH radical scavenging activity and the lowest scavenging activity of alcalase-treated peptides. Superoxide radical scavenging activity showed that bromelain treated low molecular peptide showed the highest radical scavenging activity of 50% or more. Hydroxyl radical scavenging activity ranged from about 16.73 to 69.16%, the highest among bromelain-treated low molecular peptides. $Fe^{2+}$ chelation abilities showed a distribution between about 17.85 to 47.84%. The chelation capacity of the hydrolysates was not significantly different without any difference to the enzymes used. The results of amino acid analysis showed differences between hydrolysates of alcalase, bromelain, flavourzyme, neutrase, papain, and protamex enzymes. The most amino acid was glutamic acid. The molecular weight distribution of the enzyme hydrolyzates was in the range of 300-2,000 Da, although the molecular weight distribution differed according to the treated enzymes.

• Food Science and Preservation
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• v.16 no.6
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• pp.938-945
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• 2009

The objective of this study was to investigate the effects of ingestion of low-molecular-weight collagen peptides on lipid composition, blood glucose level, and enzyme activities in the serum of hyperlipidemic rats fed experimental diets for 5 weeks. Concentrations of total cholesterol, low-density lipoprotein (LDL)-cholesterol, free cholesterol, triglyceride (TG), phospholipid (PL), and blood glucose, the atherosclerotic index, and the cholesteryl ester ratio were higher in serum of the hyperlipidemic group (CW group), and the cholesterol-plus-collagen peptides extract group (CCP group) than in the control group (BG group basal diet plus water). However, the concentrations of total cholesterol, LDL-cholesterol, free cholesterol, TG, PL, and blood glucose, the atherosclerotic index, and the cholesteryl ester ratio in serum were lower in the CCP group than in the CW group. By contrast, the ratio of HDL-cholesterol to total cholesterol and the absolute HDL-cholesterol level in the CCP group were higher than in the CW group. The activities of alkaline phosphatase (ALP) and aminotransferases (AST, ALT) in serum were lower in the CCP group than in the hyperlipidemic CW group. The results indicate that an extract of low-molecular-weight collagen peptides effectively inhibited increases in lipid elevation, blood glucose level, and enzyme activities, in the serum of hyperlipidemic rats.

• Bulletin of Food Technology
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• v.15 no.2
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• pp.3-24
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• 2002

펩타이드와 단백질은 전사, 번역, 후번역 단계에 걸친 모든 생물학적 반응을 조절한다. 그러나 분자수준에서 구조와 기능에 대한 우리의 이해는 초보적인 수준이다. 구조와 기능의 연관성에 대한 문제는 펩타이드와 단백질 자체에 대한 것과는 좀 다르다는 것을 명확히 할 필요가 있다. Multidomain을 갖는 단백질은 작고 통합적이며, 구조적으로 제한된 부분으로 쪼개는 것은 천연 단백질의 활성을 모방하여 저분자의 nonpeptide를 설계하는데 있어서 주요한 일이다. 결정적인 역할을 수행하는 domain을 모방하는 것은 특이성과 치료 효과에 있어서 자연적으로 얻어지는 단백질 물질과 비교하여 이로운 특성을 갖을 수 있고 분자 인지 분야에 관한 연구에 유용한 단서를 제공한다(Chen etal., 1992). 한편 펩타이드는 환경에 의해 구조가 심하게 영향을 받아 특성이 쉽게 변한다(Marshall et al., 1978). 수용액 내에서 이러한 구조적 유동성 때문에 그들이 결합할 수용체나 생리활성을 띄는 구조를 결정하는 것은 어렵고 복잡한 일이다(Fauchre,1987; Hruby, 1987). 구조를 한정하면 이러한 결정을 매우 쉽게 할 수 있다(Hrubyet al., 1987). 단백질 모방학은 분자지각 연구에 강력한 수단이며, 복잡한 단백질과 펩타이드의 구조-기능관계를 탐구하고 분석하는데 독특한 방법이다. 이 장에서는 매우넓고 빠르게 확장되고 있는 Peptidomimetic연구를 간략히 소개하고 있다. 단 본문은 기술 범위를 N-Methylated 아미노산과 스테로이드 등으로 제한하여 소개한다.

• Journal of Life Science
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• v.31 no.2
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• pp.219-222
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• 2021

In this study, we examined low molecular weight peptides using proteomics in order to identify their original proteins, derive their peptides, and determine the functions of the proteins in Tenebrio molitor, the mealworm (larvae, pupae, or adult) from which the peptides were extracted. Fifty-four proteins were finally identified through an analysis of proteome to derive the analyzed peptides. The proteins that induced low molecular weight peptides were identified to be the most abundant in adults only, and the next highest were derived from a group containing both adults and larva. However, other groups, including pupa, were detected to have a lower frequency of peptides. As a result of orthologous classification of the detected proteins, the general function prediction was only investigated at the highest frequency among the examined proteins. Proteins related to chromatin structure and dynamics were detected by their higher frequency among functional classes. The next highest frequency was shown by proteins related to amino acid transport and metabolism and carbohydrate transport and metabolism. Therefore, it is assumed that proteins correlated with chromatin, amino acid, and carbohydrate metabolisms are easily induced into low molecular weight peptides, and that their peptides could play a role as bioactive substances.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.10a
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• pp.131-132
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• 2001

발효식품에 함유되어 있는 저분자 peptide는 발효되는 동안이나 가수분해되는 과정에서 생산된다. 이렇게 생성된 저분자 peptide는 생물학적 활성이 크다고 보고되고 있다. 최근 농산물에 대한 peptide의 실험에 대한 연구는 활발히 진행되어 있으나 수산발호식품의 peptide의 기능성에 대한 연구는 미비한 실정이다. 현재, 암은 오늘날 급속한 의학의 발달에도 불고하고 아직도 우리의 생명을 위협하는 무서운 불치병으로 알려져 있다. (중략)

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.102-103
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• 2000

액젓은 어류, 패류, 어류의 내장 등에 소금을 가하여 부패균의 번식을 억제하고, 어패류 자체의 효소와 외부 미생물의 효소작용으로 육질을 분해시킨 후 걸러서 만든 독특한 맛과 풍미의 발효식품이다. 액젓은 숙성과정을 통하여 단백질이 폴리펩타이드 형태를 거쳐 다시 펩타이드에서 정미성분인 아미노산으로 분해되게 된다. 액젓의 주된 역할은 천연 조미의 역할이고 그 맛의 결정 요인은 저분자 질소화합물이라 할 수 있으나 핵산 관련 물질 중 맛의 주성분인 IMP는 숙성기간이 지남에 따라 분해되기 때문에 많은 영향은 미치지 않을 것으로 보여진다. (중략)

• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.4
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• pp.321-326
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• 2012

Recent studies have revealed that collagen peptide plays a protective role on skin by improving the activity of antioxidants and acts as an inducer of skin regeneration. To evaluate the efficacy of low molecular weight collagen peptide for skin recovery after non-ablative 1550 nm fractional photothermolysis laser. 10 volunteers were randomly divided into two groups. Both control and experimental groups received fractional photothermolysis treatment. In the experimental group, 5 subjects received oral collagen peptide 1,000 mg/day for 8 weeks. Before and after the treatment, we measured elastic recovery of skin, transepidermal water loss (TEWL) and erythema index (EI) for each patients. The evaluation of clinical results showed that elastic recovery of skin is higher in the experimental group than the control group (p < 0.05). TEWL have no significancy between two groups and erythema rapidly disappeared in the experimental group. On the quartile grading scale, the mean patient satisfaction 4 weeks after the fractional photothermolysis treatment was 2.0 in experimental group and 1.2 in control group. The low molecular weight collagen peptide appears to be an effective conservative therapy for skin recovery after non-ablative 1550 nm fractional photothermolysis treatment.