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Plantlet Formation and Somatic Embryogenesis from the penduncle explants of wild Corydalis remota for. peatinata (야생 현호색 (Corydalis remota for. peatinata)의 꽃자루로부터 체세포배 발생과 소식물체형성)

  • 천우재;이대원
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.1
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    • pp.15-19
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    • 1999
  • For the induction of somatic embryogenic callus, the penduncle explants of Corydalis remota for peatinata were cultured on MS basal media supplemented with 2,4-D, kinetin and zeatin. The highest embryogenic callus formation was observed on the media containing 2.0 mg/L of 2,4-D and 2.0 mg/L of zeatin. The somatic embryogenesis on the media with 0.5 mg/L of cytokinin (zeatin or kinetin) were excellent under light condition, however somatic embryos abnormally developed into plantlets. Normal dicotyledonary plantlets were found on MS medium supplemented with 1.0 mg/L of zeatin. When MS medium with 2,4-D plus cytokinin and with BAP were used, the secondary somatic embryogenesis took place in root explants of the regenerants derived from in vitro somatic embryogenic callus.

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A Basic Experimental Study on Composting of Garbage Wastes by Coconut Peat (코코넛 피트를 이용한 음식물 쓰레기의 퇴비화 기초실험)

  • Huh, Mock;Han, Ji-Yong
    • Journal of the Korea Organic Resources Recycling Association
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    • v.7 no.2
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    • pp.105-111
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    • 1999
  • This study was performed about composting in a batch reactor of laboratory scale using garbage waste and swine waste. Sawdust and coconut peat were materials to control optimum moisture, C/N ratio and specific gravity in the study. Comparing compost using only sawdust with compost using sawdust and coconut peat, the latter was higher at reduction rate and decomposition rate. Coconut peat was accelerated aerobic fermentation, because it had moisture holding ability, initial moisture was low, ventilation was good and control of optimum specific gravity was possible. Compost by only garbage waste was under standard of manure. but mixtures in same proportion of garbage waste and swine waste producted high quality compost. CEC value was average 63.8me/100g. The initial C/N ratio of compost was regulated effectively because of high C/N ratio of sawdust. As the C/N ratio(>40) was higher, reduction rate was higher. During the composting C/N ratio was improved more and more.

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심신(心腎)의 상호관계(相互關係)에 대한 동서의학적(東西醫學的) 연구(硏究) -관여심신상호관계적동서의학연구(關於心腎相互關係的東西醫學硏究)-

  • Lee, Yong-Sik;Yun, Chang-Yeol
    • Journal of Korean Medical classics
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    • v.18 no.2 s.29
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    • pp.20-44
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    • 2005
  • 통과한의학화서양의학적원리연구심신적상호관계(通過韓醫學和西洋醫學的原理硏究心腎的相互關係), 득도료여하결론(得到了如下結論) 경연구심신지간재한의학상구유(經硏究心腎之間在韓醫學上具有): 심양여신음유'심신상교'적'수화기제'관계(心陽與腎陰有'心腎相交'的'水火旣濟'關係). 차외환유신양(명문화)여심양(심화)적상호의존관계(此外還有腎陽(命門火)與心陽(心火)的相互依存關係). 우유심장신여신장정적상호의존관계이지유신정충족시(又有心藏神與腎藏精的相互依存關係而只有腎精充足時), 재가이사심주신지적공능유지정상(才可以使心主神志的功能維持正常). 경연구심신지간재서양의학상구유(經硏究心腎之間在西洋醫學上具有): 제일(第一), 심장여신장재해부학상밀절연계(心臟與腎臟在解剖學上密切聯係), 즉종심장좌심실분지출신동맥(卽從心臟左心室分枝出腎動脈), 진이형성신소구등신장적혈관구화모세혈관강(進而形成腎小球等腎臟的血管球和毛細血管綱). 제이(第二), 심장향신장공응혈액(心臟向腎臟供應血液), 신장시인체중접수혈공최다적장기(腎臟是人體中接受血供最多的臟器). 제삼(第三), 심장통과심납소(ANP)적분비(的分泌), 증가신혈류양급신소체여과율(增加腎血流量及腎小體濾過率). 제사(第四), 신장통과신소(腎臟通過腎素)(renin)적분비조절혈압(的分泌調節血壓). 제오(第五), 신공능부전대심장구성부면영향(腎功能不全對心臟構成負面影響). 제육(第六), 심장여신장지간종서양의학적각도고려(心臟與腎臟之間從西洋醫學的角度考慮), 야존재착상생화상극관계(也存在着相生和相克關係). 경연구심신적상호관계재수행중적작용(經硏究心腎的相互關係在修行中的作用), 득출여하결론(得出如下結論): 제일(第一), 수행영인안정심지(修行令人安定心志), 사심화하강(使心火下降). 제이(第二), 수행시적호흡조절조폐금생신수(修行時的呼吸調節助肺金生腎水), 저시소유적수행법도재중시호흡조절적근본이유(這是所有的修行法都在重視呼吸調節的根本理由). 제삼(第三), 수행시발생수승화강(修行時發生水昇火降). 저실제상시의뢰심화중적일음효여신수중적일양효적발동내완성(這實際上是依賴心火中的一陰爻與腎水中的一陽爻的發動來完成). 제사(第四), 통과수행(通過修行), 가이달도보정적목적(可以達到保精的目的), 유차보장생명적지보(由此保藏生命的至寶). 제오(第五), 통과수행(通過修行), 가이달도연정화기(可以達到煉精化氣), 약능주도연정화기(若能做到煉精化氣), 가사인체불누설정액(可使人體不漏泄精液), 반이화기이증강인체기능(反而化氣以增强人體機能), 시보정적유효수단(是保精的有效手段). 수행적관건시'남자수성불누정(修行的關鍵是'男子修成不漏精), 여자수성온불누경(女子修成不漏經)'.

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Comparative Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Glehnia littoralis Schmidt et Miquiel (갯방풍(Glehnia littoralis Schmidt et Miquiel)에서 캘러스 유도 및 식물체 재분화에 미치는 생장조절제의 효과)

  • 이병국;한미숙;정양균;나의식;윤성중;유남희
    • Korean Journal of Plant Resources
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    • v.17 no.2
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    • pp.153-160
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    • 2004
  • Glehnia littoralis is known as an edible and medicinal plant using green loaves and mature roots of plant. In the present paper, the influence of plant growth regulators on callus induction and plant regeneration was investigated. Callus induction and regeneration occurred from leaf and petiole explants in Glehnia littoralis. Optimal condition of plant growth regulators for callus induction from leaf and petiole explants was MS basal medium supplemented with 2mg/L 2,4-D and 2mg/L BA. The frequency of callus induction was higher in petiole explant than leaf. When the callus was cultured on MS basal medium supplemented with 0∼1 mg/L IAA, 0∼1mg/L NAA and 0∼2mg/L BA for about 65 days, the most effective plant growth regulators on plant regeneration from callus were 1mg/L NAA and 2mg/L BA. The plantlets acclimatized successfully and grown in vermiculite matrix.

The Influence of Message Types of Comparative Advertising on Advertising Effect (비교광고의 메시지 유형에 따른 광고효과 분석)

  • Moon, Jae-Hak
    • Management & Information Systems Review
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    • v.31 no.4
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    • pp.639-661
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    • 2012
  • The purpose of the paper is to examine sidedness effects of comparative advertising and moderating roles of the emotions of customer and product type. To test the research hypotheses, this study conducted experimental designs. One hundred ninety-two undergraduate students were assigned to one of the four experimental groups. Comparative Advertising message type and product type were manipulated as between-subject factors. There was a surprising event situation(free gift event) when participants came to laboratory for experiment, which is for the emotion manipulation. If a participant in a positive emotion group, he or she gets a free gift. The data demonstrate that comparative message type have a significant effect on both attitude toward the advertising and acceptance intention. In addition, consumers' emotion and product type play a significant moderating role between the message types and the dependent variables. The results of this study contribute to the literature as a new attempt to examine the sidedness effect of the comparative advertising message. We suggested that message types and consumer's emotion is one of very important aspects for a comparative advertising and marketers should consider people's emotion when they release a comparative advertising, which can help when marketers make their strategy for a comparative advertising.

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A small Inverted-F Antenna with adjustable characteristics using lumped elements (집중소자를 이용하여 특성 조절이 가능한 소형 역-F 안테나)

  • Yoo, Jin-Ha;Do, Sang-In;Lee, Young-Soon
    • Journal of Advanced Navigation Technology
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    • v.17 no.6
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    • pp.646-651
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    • 2013
  • In this paper, a small inverted-F antenna with adjustable characteristics is proposed with use of lumped elements, capacitor and resistor. Capacitor is inserted between end of the antenna and ground for size reduction and tuning of resonant frequency. On the other hand, $0{\Omega}$ resistor is replaced as the short line connected to ground. The movement of short point due to use of $0{\Omega}$ resistor results in providing variation of input impedance. Therefore optimum characteristics can be obtained only by proper choice of capacitor and short point without redesign of it's geometry. In order to check the validity, the proposed antenna is designed and fabricated for 2.4 GHz frequency band, and then is applied to a product of Zigbee wireless remote controller. As a result, the size of applied antenna is $8.5{\times}4.5mm^2$ and it is observed that the measured bandwidth and antenna gain are 150 MHz and 2 dBi respectively without redesign of the antenna.

Control of Daily Integral PPE by the Artificial Lighting and shading screen In Greenhouse (인공광 및 차광스크린을 이용한 온실의 일일적산 광합성유효광량자속 조절)

  • 이현우
    • Journal of Bio-Environment Control
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    • v.12 no.1
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    • pp.45-53
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    • 2003
  • The object of this study was to develop the control technology of daily integral photosynthetic photon flux (PPF) by the artificial lighting and shading screen in greenhouse. The shading time needed to get the target PPF by using two types of shading screens having shading ratio of 55% and 85% was analyzed. The results showed the shading ratio of screen to be installed in greenhouse should be different depending on the amount of target PPF to be controlled. The PPF control experiment by using the 55% shading screen in July and August showed that the maximum difference between measured and calculated value was about 5 mol$.$ $m^{-2}$ $.$ $d^{-1}$ in no shading condition. This difference is satisfactory result because the daily integral PPF is quite different depending on the weather condition. The simulation result about PPF distribution pattern shortened the time needed to find the proper arrangement of artificial lightings in greenhouse. But the further study was required to find the supplemental lighting arrangement to be able to provide the exactly uniform distribution of target light intensity. The supplemental irradiation time needed to acquire the target daily integral PPF for different supplemental light intensities, weather conditions, and months was analyzed. The result showed that the supplemental light intensity should be decided depending on the amount of target PPF to be controlled. The result of PPF control experiment conducted by using 55% shading screen and 300 $\mu$mol$.$ $m^{-2}$ $.$ $s^{-1}$ supplemental light intensity from the end of May to the beginning of June showed that the maximum difference between target and measured value was about 3 mol$.$ $d^{-1}$ $.$ $m^{-2}$ . If we consider that the difference of the daily integral PPF depending on weather condition was the maximum 30 mol$.$ $m^{-2}$ $.$ $d^{-l}$, the control effect was acceptable. Although the result of this study was the PPF control technology to grow lettuce, the data and control method obtained could be employed for other crop production.n.

The Study on the Different Moderation Effect of Contingency Variable (Focused on SPSS statistics and AOMS program) (상황변수의 조절효과 차이에 관한 연구 (SPSS와 AMOS프로그램을 중심으로))

  • Choi, Chang-Ho;You, Yen-Yoo
    • Journal of Digital Convergence
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    • v.15 no.2
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    • pp.89-98
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    • 2017
  • This study analyzed empirically the same data through SPSS statistics(regression analysis) and AMOS program(structural equation model) used for cause and effect analysis. The result of empirical analysis of moderation effect was as follows. Meanwhile, SPSS statistics(regression analysis) did not pictured moderation effect in the categorical data(sex) and continous data(satisfaction of consunting), AMOS program(structural equation model) pictured partial moderation effect about the effecting of consultant's capability and attitude on the consulting repurchase within 10% level of significant. Eventually, This study showed that AMOS program and SPSS statistics used different methology in moderation effect, thus the different outcomes appeared although using the same data.

Plant Regeneration from Hypocotyl-Derived Protoplasts of Brassica oleracea var. capitata (양배추 배축 원형질체로부터 식물체 재분화)

  • 이연희;조현석;서석철;김호일
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.1
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    • pp.7-11
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    • 1995
  • Protoplasts were isolated from hypocotyl tissues of 5-day-old Brassica oleracea var capitata Green Challenger seedlings. Several media were used for protoplast culture and shoot regeneration. The shoot-regeneration rapacity of protoplast derived callus depended on the initial culture medium. Protoplasts were cultured in liquid medium (B5 medium supplemented with CaCl2, 2H2O 600mg/L, g1ucose 20g/L, D-mannito1 70g/L, NAA lmg/L, BA lmg/L, 2.4-D 0.25 mg/L)at 27$^{\circ}C$ under the dark After 5 to 10 days, cultlues were diluted with medium with a reduced osmotic stabilizer and then transferred to illuminated conditions. The culture medium was changed with the fresh medium at 7- to 10-day-intervals until the formation of microcallus. Hypocotyl protoplast-derived callus proliferated when transferred to MS medium supplemented with NAA lmg/L, BA 1mg/L and GA$_3$ 0.02mg/L. Upon transfer to MS basal medium without growth regulators, roots were produced. In an attempt to increase the regeneration frequency, 10g/L polyvinylpyrrolidone was added to the regeneration medium, but the shoot regeneration was mot improved. The regenerated whole plants were acclimated in a sterized soilless mixture(vermiculite 2;perlite 2;peat moss1) in a culture room.

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Characterization of Controlled Low Strength Materials Utilizing CO2-fixation Steel Slag and Power Plant Bottom Ash (CO2고정화한 제강슬래그와 발전소 바닥재를 활용한 저강도 고유동 채움재의 특성)

  • Cho, Yong-Kwang;Kim, Chun-Sik;Nam, Seong-Young;Cho, Sung-Hyun;Lee, Hyoung-Woo;Ahn, Ji-Whan
    • Journal of Energy Engineering
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    • v.27 no.2
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    • pp.55-60
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    • 2018
  • In this study investigated the Controlled Low Strength Materials using coal ash and steel slag(KR slag) as the main material in the thermal power plant classified as waste resource. Bottom ash and KR slag are mixed at a ratio of 7: 3 to expand the use of industrial by-products through carbonate($CO_2$-fixation) reactions and inhibit the exudation of heavy metals. The results showed that the water content increased as the content of bottom ash increased. It was confirmed that as the powder content increased, the bleeding ratio decreased. Also, as the content of one kind of ordinary portland cement (OPC) decreased, activation of hydration reaction decreased and compressive strength decreased. However, when the mixed composition is appropriately adjusted, the compressive strength of 2.0 MPa required for the controlled low-strength material can be satisfied.