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Histological Studies on the Exuvial Gland in a Non-moulting Silkworm, Bombyx mori L (회피불능잠의 피선에 관한 조직학적 연구)

  • 윤종관;사기언
    • Journal of Sericultural and Entomological Science
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    • v.16 no.2
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    • pp.119-125
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    • 1974
  • When the nomal silkworms reached active time of 3rd instar stage both non-moulting larva and normal silkworms from the same rearing tray were collected and fixed. The silkworms in 4th instar stage whose growth was as dwarfish as those in 1st and 2nd instar stages were also collected and fix with the normal silkworms. Non-moulting larva and normal silkworms were morphologically compared and the examined results from the tissue inspection are summarized as follows: 1. In spite of the fact that the normal silkworms reached the active eating time of 3rd instar stage non-moulting silkworms were dwarfish as if they had been reared for two days. Non-moulting silkworms which were observed at the time of 4th instar stage showed no much difference in their growth. 2. There was the tendency that the exuvial gland as was shown in Fig. 1 and 2 was flat cyslidium of ellipse and its size at thorax was small shile the gland at abdomen was big. 3. The exuvial gland at thorax has been reported to be bigger at thoracic base than at dorsal vessel but according to the present it was examined to be irregular. 4. The size of exuvial gland of silkworms in the active eating stage of 3rd instar was from 151.3${\mu}$ (major axis) to 94.5${\mu}$ (minor axis) at prothorax and from 568.6${\mu}$ (major axis) to 495.1${\mu}$ (minor axis) at 7th abdominal segment. The sire oe exuvial gland of non-moulting silkworm was 57.5${\mu}$ (major axis) to 51.3${\mu}$ (minor axis) at prothorax and from 91.5${\mu}$ (major axis) to 75.5${\mu}$ (minor axis) at 5th abdominal segment (see Fig. 1) 5. When the normal silkworms reached 4th instar active eating stage its exuvial gland was compared to that of dwarfish silkworm. The result was that the size of normal silkworm at prothorax was from 252.2${\mu}$ (major axis) to 131.6${\mu}$ (minor axis) and the size of exuvial gland at 7th abdominal segment was from 691.5${\mu}$ (major axis) to 493.4${\mu}$ (minor axis) while the sire of exuvial gland of non-moulting at prothorax was from 71.4${\mu}$ (major axis) to 61.5${\mu}$ (minor axis) and the size of the non-moulting silkworm's 8th abdominal segment was from 94.6${\mu}$ (major axis) to 71.5${\mu}$ (minor axis) (See Table 2) 6. There was a remarkable difference in the from of exuvial gland of non-moulting silkworm. The size of alveolar of the non-moulting silkworm was many times larger compared to that of normal silkworm 7. There was no great difference between secretory cells of normal and non-moulting silkworms but the granular type exuvial gland was small in sire compared to that of normal silkworm.

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The Development Properties of Sericin Jam and Composition of Amino Acid in Hemolymph (세리신잠(Nd-s)의 발육특성과 혈액아미노산 조성)

  • Kim, Jong-Hyun;Sohn, Hae-Ryong
    • Current Research on Agriculture and Life Sciences
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    • v.20
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    • pp.55-63
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    • 2002
  • This experiment was to know properties of Sericin Jam that development, growth of silkgland, content of sericin and composition of amino acid in hemolymph. The characters of Sericin Jam can he seen form this experiment. Hatching ratio was 85% in Sericin Jam and 95% in Jam 120. Especially hatching period of Sericin Jam was longer than Jam 120 and also hatching of Sericin Jam was ununiform. The larval duration of Sericin Jam was 20 days and 23 hours, and Jaw 120 was 21 days and 22 hours. In Sericin Jam, middle silkgland contain a great p arts in silkgalod and posterior silkgand is short and no curves. The period of mounting to emergence was 12 days in Sericin Jam. The period of pupa was 7day. It is property of Sericin Jam that the period is short. Cocoon was very thin and light in Sericin Jam. Weight of cocoon shell of Sericin Jam is 2.7cg. The sericin protein quantity was 100% in cocoon shell of Sericin Jam, about 28% in cocoon shell of Jam 120, however the sericin ratio per Sericin Jam cocoon was 34.6% compare to cocoon shell of Jaw 120 in sericin protein quantity. The amino acids in hemolymph of Sericin Jaw was much hidtidine, lysine, glut amic acid. And the amount of almost amino acids were increased depends upon development at t he 5th instar.

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Physicochemical Characteristic of the Silkworm Sericin Cocoon (세리신잠견의 이화학적 특성)

  • 김수연;손해룡;배도규;김정호
    • Journal of Sericultural and Entomological Science
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    • v.45 no.1
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    • pp.10-17
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    • 2003
  • This study was carried out to analyze physicochemical characteristics of sericin cocoon from silkworm, Bombyx mori. The degumming loss increased with increasing treatment time up to 2 hr, and temperature up to 130$^{\circ}C$. At 130$^{\circ}C$, degumming loss of Nd-s jam and Nd$\^$H/ jam were 100% while that of Baegok jam was 24%. Nd-s jam and Baegok jam ha high glycine content of 29.1∼46.3 mol% where as Nd$\^$H/ jam had high serine content of 32.6 mol%. Thermal denaturation temperatures were found at 218$^{\circ}C$ for Nd-s jam, 216$^{\circ}C$ for Nd$\^$H/ jam, and 218$^{\circ}C$ for Baegok jam. Before degumming, crystallinities obtained by FT-IR analysis were 44.3, 43.7, and 59.9% for Nd-s jam, Nd$\^$H/ jam, and Baegok jam respectively. After degumming, crystallinity increased to 61.8% for Baegok jam. Before degumming, crystallinitics obtained from XRD were 35.9, 33.5, and 47.2%, for Nd-s jam, Nd$\^$H/ jam, and Baegok jam. After degumming, crystallinity increased to 49.8% for Baegok jam. The molecular weight of Nd$\^$H/ jam were 9,417 in 1 hr, 3,744 in 2 hr, 4,944 in hr, and 3,910 in 6 hr.

Breeding of (다수성 편친한성(片親限性) 가을누에 품종)

  • Kang, Pil-Don;Sohn, Bong-Hee;Lee, Sang-Uk;Kim, Mi-Ja;Jung, I-Yeon;Kim, Young-Soon;Kim, Yung-Dae;Lee, Heui-Sam
    • Journal of Sericultural and Entomological Science
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    • v.46 no.2
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    • pp.54-57
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    • 2004
  • A new silkworm variety "Buhungjam" for autumn rearing season is $F_1$ hybrid between Jam149, a japanese race bred from introduction breeding and Jam150, a chinese race from introduction breeding. Jam149, japanese parent of the "Buhungjam" showed high GCA in pupation percentage, cocoon shell percentage and filament length and Jam15, chinese parent showed high GCA in cocoon shell percentage and cocoon filament length. In the local adaptability test performed at 8 local areas in spring of 2003, Buhungjam records 7% higher in cocoon yield and 8% in raw silk yield than the check variety, Daesungjam.

The Developmental Characterization of the Sericin jam(Bombyx mori), a Mutant in the Synthesis and Secretion of Fibroin (세리신잠(Nd-s. $Nd^H$의 발육 특성)

  • 김수연;손해룡
    • Journal of Sericultural and Entomological Science
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    • v.45 no.1
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    • pp.6-9
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    • 2003
  • This experiment was done to know developmental characteristics of Sericin jam. Incubation periods were 10 day 2 hr, and 10 day 1 hr. 11 day 1 hr for Nd-s jam, N $d^{H}$ jam, and Baegok jam, respectively. Hatching rates were 83.9, 83.3 and 96.0% for Nd-s jam, N $d^{H}$ jam, and Baegok jam. Larval periods were, 20 days 1 hr for Nd-s jam, 20 days 5 hrs for N $d^{H}$ jam, and 22 days 12 hrs for Baegok jam. Death rate of larvae was highest in N $d^{H}$ iam, followed by Nd-s jam and Baegok jam. Pupation rate was highest in Baegok Jam followed by Nd-s jam and that of N $d^{H}$ jam was the lowest among the three. Cocoon weight was 1.39, 1.08, and 2.01 g for Nd-s jam, N $d^{H}$ jam, and Baegok jam, respectively. Shell weight were 13, 3, and 48 cg for Nd-s jam, N $d^{H}$ jam, and Baegok jam. Cocoon shell ratios were 9.0% for Nd-s jam, 2.8% for N $d^{H}$ jam and 23.9% for Baegok jam. Cocoon sizes were 30.6${\times}$15.8 mm for Nd-s jam, 24.7${\times}$14.9 mm for N $d^{H}$ jam and 35.8 ${\times}$ 20.5 mm(1${\times}$w) for Baegok jam.w) for Baegok jam.

Development of Mission Language for Autonomous Underwater Vehicle (자율무인잠수정을 위한 임무 언어 개발)

  • Kim, Bang-Hyun;Lee, Fill-Youb;Sim, Hyung-Won;Jun, Bong-Huan;Lee, Pan-Mook
    • Proceedings of the Korean Information Science Society Conference
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    • 2010.06c
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    • pp.554-559
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    • 2010
  • 자율무인잠수정은 탐사 목적에 따라 다양한 임무를 수행해야 하며, 임무에 따라 자율무인잠수정 행동의 유형과 순서는 달라질 수 있다. 그러나 대부분의 자율무인잠수정은 한정된 임무에 대하여 프로그램 내부에 고정된 행동 유형으로 동작하며, 다른 유형의 임무를 수행해야 할 경우에는 프로그램을 수정해야 하는 문제점이 있다. 따라서 본 연구에서는 자율무인잠수정이 수행할 수 있는 다양한 임무를 명시할 수 있는 임무 언어를 개발하였다. 이 임무 언어는 명령어의 실행 순서를 제어할 수 있는 제어문과 자율무인잠수정의 행동을 지정하거나 자율무인잠수정의 상태를 입출력 할 수 있는 명령어, 그리고 변수 정의를 제공하기 때문에, 사용자가 자율무인잠수정의 임무를 자유롭게 표현하는 것이 가능하다. 임무 언어로 작성된 임무 파일은 전용 어셈블러에 의해 이진 형식의 실행이미지로 변환된 후에, 자율무인잠수정 내장 소프트웨어 내부의 가상기계 기억장치에 적재되어 실행된다. 실행이미지를 가상기계에서 해석하고 실행하는데 필요한 시스템의 자원을 최소화하기 위하여 임무 언어는 자율무인잠수정의 임무를 표현하기 위한 필수적인 부분만을 고려하여 설계되었으며, 문법은 ARM v5 어셈블리와 유사한 형태이다. 개발된 임무 언어는 한국해양연구원에서 개발한 이심이100 자율무인잠수정에 적용되었으며, 이후 개발할 6,000m급의 이심이6000 자율무인잠수정에도 사용될 예정이다.

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Comparison of Economical Character of the Silkworm, Bombyx Mori L., Introduced from Foreign Sericultural Countries to Turkey (터어키에 도입된 잠품종의 실용형질의 비교에 관한 연구)

  • Ryu, Keun-Sup
    • Journal of Sericultural and Entomological Science
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    • v.21 no.1
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    • pp.46-49
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    • 1979
  • These experiments were made in order to compare the productivity of various varieties of F$_1$silkworm eggs imported from foreign sericultural countries with the productivity of some F$_1$silkworm eggs varieties produced by Turkey. The results are summarized as follows: 1. In the durations of whole instar, it lasted 30∼38 days under natural rearing conditions but Jam 107${\times}$Jam 105 about 30 days. 2. Cocoon yield per case of silkworm eggs was shown significantly among the varieties. Thaiei ${\times}$ Choan was the highest yield of cocoon, followed by Isonzo, Piave, M-198, M${\times}$N, S${\times}$P, J${\times}$C and Jam 107${\times}$Jam 108 in that order. 3. In the percentage of cocoon shell, M-197 was the highest and other varieties were almost on the same level of cocoon shell, but Jam 107${\times}$ Jam 108 was the lowest percentage. 4. In the weight of a cocoon, Choan${\times}$Thaiei was the heaviest, followed by S${\times}$P, C${\times}$J, M${\times}$N, M-197, Piave, Livenzo and Jam 107${\times}$Jam 108 in that order. 5. In the raw silk percentage of fresh cocoon, Choan${\times}$Thaiei, 3 Italian varieties and Turkish M${\times}$N. C${\times}$J belonged to the first group of better raw silk percentage, followed Polish M-197 and Jam 107${\times}$1am 108 in that order.

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About the character of next generation in the poly-generous ploy copulation. (동종품 교배와 이품종 교배를 교번한 이중교배의 차대 잠형질에 관한 연구(IV) -다원다교배와 차대잠 형질-)

  • 김윤식
    • Journal of Sericultural and Entomological Science
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    • v.7
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    • pp.43-47
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    • 1967
  • The author studied on the character of next generation which was laid from poly copulated female moth. The obtained results are as follows; 1. In the three generous two copulation, selectivity of fertilization and copulating order of silkworm showed like the two generous double copulation and their intensity of fertilization was characterized in the next generation. 2. In the next generation of silkworm, new form and character were not occurred through polygenerous poly copulation. 3. Copulating time influenced on the next generation and the time was related closely tn the copulating type (copulating order) in the three generous two copulation which was differed each copulating time.

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Physicochemical and Sensory Characteristics of Tarakjuk with Stachys sieboldii Miq Root Powder (초석잠 뿌리 분말을 첨가한 타락죽의 이화학적 및 관능특성)

  • Tae, Mi Hwa;Kim, Kyoung Hee;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.6
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    • pp.859-864
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    • 2016
  • The study investigated the physicochemical and sensory characteristics of Tarakjuk added with various concentrations of Stachys sieboldii Miq root. Tarakjuk prepared by addition of 0, 10, 20, and 30% powder to rice flour basic formulation. The spreadability and moisture content of Tarakjuk increased with addition of Stachys sieboldii Miq root powder, whereas viscosity decreased with addition of Stachys sieboldii Miq root powder. The pH of Tarakjuk decreased with addition of Stachys sieboldii Miq root powder, whereas acidity increased. The lightness of Tarakjuk decreased with addition of Stachys sieboldii Miq root powder, whereas redness and yellowness increased with addition of Stachys sieboldii Miq root powder. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activities of Tarakjuk significantly increased as Stachys sieboldii Miq root powder content increased (P<0.05). The results of the sensory evaluation showed that the flavor and taste of Tarakjuk were not significantly different among the samples. Appearance, aftertaste, texture, and overall acceptability showed the highest scores for Tarakjuk containing 10% Stachys sieboldii Miq root powder. Based on the results of this study, Tarakjuk added with 10% Stachys sieboldii Miq root was determined to be optimum for its good characteristics and overall acceptability.

Histological Observations and Comparison of the Resistance to Polyhedrosis Viruses in Various Varieties of the Silkworm, Bombyx mori L., fed on Artificial Diet (가잠의 인공사료육에 있어서 다각체 바이러스에 대한 품종별 저항성 및 조직학적 관찰)

  • Gang, Seok-U;Im, Jong-Seong;Son, Hae-Ryong
    • Journal of Sericultural and Entomological Science
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    • v.27 no.2
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    • pp.20-27
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    • 1985
  • This study was carried out to investigate the histological changes after the infection of nuclear and cytoplasmic polyhedrosis viruses(NPV, CPV) and the resistance to the viruses in various varieties of the silkworm fed on artificial diet. The results obtained were as follows; Among four varieties of silkworm tested, Jam 107$\times$Jam 108 was more resistant than the other varieties tested and Jam 119$\times$Jam 120 was the most resistant to CPV. In case of peroral infection with NPV, Jam 107$\times$Jam 108 showed lower mortality than the remained varieties in low concentration (104/ml). However, all varieties showed high mortality as the concentration of viruses was increased. With infection of CPV, the varieties showed high mortality at the concentration of 107 and 108/ml, while Jam 119$\times$Jam 120 showed the lowest mortality at virus concentration of 104/ml. The fat bodies, epidermal cells and tracheal epithelial cells showed high susceptibility to NPV to break the cells completely and liberate the debris to the body cavity. The CPV infected only the cylindrical cells of mid-gut and formed polyhedrons. In some cells, CPV was liberated to gastral cavity. In the electrophoretic pattern of hemolymph protein of silkworm larvae infected with NPV, bands were dimmed and disappeared as symptom aggravated after infection. Electrophoretic pattern of homolymph proteins of silkworm larvae infected with CPV showed no numerical difference at the later stage of infection, and one or two bands was observed along with lowering the concentrations.

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