Histological Studies on the Exuvial Gland in a Non-moulting Silkworm, Bombyx mori L

회피불능잠의 피선에 관한 조직학적 연구

  • Published : 1974.12.01

Abstract

When the nomal silkworms reached active time of 3rd instar stage both non-moulting larva and normal silkworms from the same rearing tray were collected and fixed. The silkworms in 4th instar stage whose growth was as dwarfish as those in 1st and 2nd instar stages were also collected and fix with the normal silkworms. Non-moulting larva and normal silkworms were morphologically compared and the examined results from the tissue inspection are summarized as follows: 1. In spite of the fact that the normal silkworms reached the active eating time of 3rd instar stage non-moulting silkworms were dwarfish as if they had been reared for two days. Non-moulting silkworms which were observed at the time of 4th instar stage showed no much difference in their growth. 2. There was the tendency that the exuvial gland as was shown in Fig. 1 and 2 was flat cyslidium of ellipse and its size at thorax was small shile the gland at abdomen was big. 3. The exuvial gland at thorax has been reported to be bigger at thoracic base than at dorsal vessel but according to the present it was examined to be irregular. 4. The size of exuvial gland of silkworms in the active eating stage of 3rd instar was from 151.3${\mu}$ (major axis) to 94.5${\mu}$ (minor axis) at prothorax and from 568.6${\mu}$ (major axis) to 495.1${\mu}$ (minor axis) at 7th abdominal segment. The sire oe exuvial gland of non-moulting silkworm was 57.5${\mu}$ (major axis) to 51.3${\mu}$ (minor axis) at prothorax and from 91.5${\mu}$ (major axis) to 75.5${\mu}$ (minor axis) at 5th abdominal segment (see Fig. 1) 5. When the normal silkworms reached 4th instar active eating stage its exuvial gland was compared to that of dwarfish silkworm. The result was that the size of normal silkworm at prothorax was from 252.2${\mu}$ (major axis) to 131.6${\mu}$ (minor axis) and the size of exuvial gland at 7th abdominal segment was from 691.5${\mu}$ (major axis) to 493.4${\mu}$ (minor axis) while the sire of exuvial gland of non-moulting at prothorax was from 71.4${\mu}$ (major axis) to 61.5${\mu}$ (minor axis) and the size of the non-moulting silkworm's 8th abdominal segment was from 94.6${\mu}$ (major axis) to 71.5${\mu}$ (minor axis) (See Table 2) 6. There was a remarkable difference in the from of exuvial gland of non-moulting silkworm. The size of alveolar of the non-moulting silkworm was many times larger compared to that of normal silkworm 7. There was no great difference between secretory cells of normal and non-moulting silkworms but the granular type exuvial gland was small in sire compared to that of normal silkworm.

정상잠이 3령의 성식기에 이르렀을때(춘잠기 소잠후약 9일 20시간 경과한 무렵) 불면잠으로 인정되는 것과 같은 잠박에서 정상잠을 재료로 하여 이를 고정하였고 또한 4령의 성식기에 1~2령기의 발육정도 밖에 되지 않은 왜소잠을 수집하여 고정하고 이와 동시에 이때의 정상잠도 고정하였다. 불면잠과 정상잠을 형태학적으로 비교하고 그 조직검사에서 얻은 조사내용을 요약하여 보면 다음과 같다. 1. 정상잠이 3령의 성식기에 이르렀음에도 불면잠으로 인정되는 누에는 마치 소잠후 2일이 경과한 정도의 누에의 크기에 불과하였으며 4령의 성식기에 관찰한 불면잠도 이와 대동소리라하였다. 2. 태피선은 타원형의 편평한 낭상체로서 그 크기는 흉부에 위치하는 것이 작고 복부의 것이 일반적으로 큰 경향을 나타냈다. 3. 흉부에 있는 태피선은 흉지의 기부에 있는 것이 배맥관을 끼고 있는 태피선보다 큰것으로 종내에는 보고되었지만 본실험결과에 었어서는 일정한 경향이 없었다. 4. 정상잠이 3령의 성식기에 이르렀을 때의 태피선의 크기는 전흉에 있는 것이 최소로서 151.3$\mu$(major axis)~94.5$\mu$(minor axis)였고 최대로서는 복부제7절의 것이 568.6$\mu$~495.$\mu$였으며 이때에 불면잠으로 인정되는 것은 최소로서 전흉에 있는 태피선이 57.5~51.3$\mu$이였으며 최대로서는 복부제5절의 것이 91.5~75.5$\mu$으였다(표 1. 참조) 5. 정상잠이 4령의 성식기에 이르렀을 때 정상잠과 왜소잠의 태피선의 크기를 비교해 본 바에 의하면 정상잠에 있어서는 전흉의 것이 최소로서 252.2~131.6$\mu$이였고 복부제7절의 것이 최대로서 691.5~493.4$\mu$이였으며 왜소잠에 있어서는 후흉의 것이 최소로서 71.4~61.5$\mu$이였고 복부제8절의 것이 최대로서 94.6~71.5$\mu$이였다(표 2. 참조) 6. 불면잠에 있어서의 태피선의 형태는 그 공포형태피선이 정상잠의 것과 크게 차이가 있어 공흉의 크기가 정상잠의 것에 비하여 몇배에 달하였다. 7. 불면잠과 정상잠과의 분필세포의 구조에는 큰 차이가 없지만 불면잠의 과립형태피선은 정상잠에 비하여 소형이 었다.

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