• Title/Summary/Keyword: 자유-자유보

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Separation of Lentil Lectin Using Free-Flow Electrophoresis (자유유동 전기이동을 이용한 Lentil Lectin의 분리)

  • 류화원;이동일장호남
    • KSBB Journal
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    • v.9 no.2
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    • pp.115-121
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    • 1994
  • A Purification device with 30-channel free-flow electrophoresis was assembled to treat samples of 240m1 volume for purification of lentil lectin (LcH) from lentil seeds with no impurities in a silverstained PAGIEF gel. HEPES(50mM)-Ttis(50mM), Cycloserine(50mM)-urea(3M), Histidine(50mM)-urea(3M) were used as ampholytea among which Histidine(50mM)-urea(3M) (pI 7.65) was found best in resolution. LcH is known to be present in the form of LcH-A, LcH-B and the complex of the two. The complex, however, disappeared when urea was added in the ampholytes, which suggested that the complete purification of two isolectins is possible using the present multistep purificaton device.

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AN ESR STUDY OF AMINO ACID AND PROTEIN FREE RADICALS IN SOLUTION PART I. Reaction Mechanism of Free Radical Production in the Ti-$H_2O_2$ Flow System (용액에서의 아미노산 및 단백질 자유기에 관한 ESR 연구 제1보 ; Ti-$H_2O_2$ Flow System에서 자유기를 만드는 반응기구)

  • Hong Sun-Joo;L. H. Piette
    • Journal of the Korean Chemical Society
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    • v.15 no.1
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    • pp.37-44
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    • 1971
  • The reaction of amino acids and the reactive hydroxyl radical generated by $Ti^{3+}-H_2O_2$ system was studied using fast flow techniques coupled with ESR. Upon adding methionine to the 0.2M $H_2O_2$ solution (0.05M methionine after addition) and mixing with 0.01M $TiCl_3$, the low field component of the two incompletely resolved peaks, in the spectrum of $Ti^{3+}-H_2O_2$ system alone, vanished completely whereas the high field component remained almost constant and superimposed on the secondary spectrum of the methionine free radical. Similar results were obtained for other amino acids and proteins. The results strongly demonstrate that the $T^{3+}-H_2O_2$ flow system generates two different radical species, only one of which, giving rise to the low field component, is alone responsible for abstracting hydrogen atoms from substrate molecules. The effects of HCl, $H_2SO_4$ and NaOH on the system were also studied with widely varying results.

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Analysis of Graph Mining based on Free-Tree (자유트리 기반의 그래프마이닝 기법 분석)

  • YoungSang No;Unil Yun;Keun Ho Ryu;Myung Jun Kim
    • Proceedings of the Korea Information Processing Society Conference
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    • 2008.11a
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    • pp.275-278
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    • 2008
  • Recently, there are many research of datamining. On the transaction dataset, association rules is made by finding of interesting patterns. A part of mining, sub-structure mining is increased in interest of and applied to many high technology. But graph mining has more computing time then itemset mining. Therefore, that need efficient way for avoid duplication. GASTON is best algorithm of duplication free. This paper analyze GASTON and expect the future work.

Rawls' Liberal Theory of Justice and Ecological Justice (롤즈의 자유주의적 정의론과 생태적 정의)

  • Choi, Byung-Doo
    • Journal of the Korean association of regional geographers
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    • v.11 no.4
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    • pp.476-496
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    • 2005
  • This paper considers some significances and limitations in application of Rawls' liberal theory of justice to conceptualization of environmental and ecological justice. It first reviews Rawls' liberal theory of justice, then examines how his theory of justice considers natural resources and can be applied to develop distributive theory of environmental justice, and finally explores what kinds of problems would be generated when Rawls' theory of justice is to be extended to animals and other natural components. In Rawls' theory of justice the status of natural resources is described as a objective condition for justice, but it is argued that natural resources should be seen as a substantial component of just production and distribution. It also can be stressed that his theory of justice does not secure a just result of resource distribution, even though it has a great significance for just procedure of distribution. Finally, it seems hardly possible to extend Rawls' theory of justice to animals and other natural components to develop a Rawlsian theory of ecological justice, because liberalism and Western tradition of science on the basis of which Rawls' theory of justice has developed presuppose an ontological dualism of human beings and other natural components, and hence cannot recognize the inherent value of nature.

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ADVANTAGE OF USING FREE NETWORK ADJUSTMENT TECHNIQUE IN THE CRUSTAL MOVEMENT MONITORING GEODETIC NETWORKS

  • AhmedM.Hamdy;Jo,Bong-Gon
    • Journal of the Korean Geophysical Society
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    • v.6 no.1
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    • pp.1-11
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    • 2003
  • There are numerous adjustment techniques that deal with the adjustment of geodetic networks but the least squares adjustment is the most common one. During the network adjustment procedure two techniques can be used, the free network adjustment technique and the constrained network adjustment technique. In order to determine the optimum technique for adjusting the geodetic networks, which used for the geodynamical purposes, data from two different geodetic networks "Sinai geodetic network, Egypt, and HGN network, South Korea" had been examined. The used networks had a different configuration and located in different areas with different seismic activity. The results show that both techniques have a high accuracy and no remarkable differences in terms of RMS. On the contrary, the resulted coordinates shows that the constrained network adjustment technique not only cause a remarkable distortion in the station final coordinates but also if the fixed points that define the datum parameters are changed different solutions for the coordinates will be determined. This distortion affect not only in the determination of point displacement but also in the estimation of the deformation parameters, which play a significant role in the geodynamical interpretation of results. Comparing the results which obtained from both techniques with the widely known geodynamical models of the area reviles that the free network adjustment technique results are clearly match with these models, while those obtained from the constrained technique didn’t match at all. By considering the results it seams to be that the free network adjustment technique is the optimum technique, which can be used for the geodetic network adjustment.

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Simulation for the Analysis of the Beam-Decking Connector Model for the Wood Dome Structure (목조 돔 구조물의 해석을 위한 보-데크 연결 모델의 정식화)

  • 김재열
    • Computational Structural Engineering
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    • v.10 no.4
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    • pp.34-40
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    • 1997
  • 본 연구의 주 목적은 돔형 구조물의 해석에 있어서 보-데크 연결 모델을 정식화하는데 있다. 목조 돔 구조물의 데크지붕 효과는 Wu(1991), Telang(1992) 등에 의해 제안되었으며, 데크지붕 효과를 설명하기 위해 트러스 브레이싱법을 도입하였다. 그러나 이 TB법은 좁은 간격으로 박혀있는 못 들의 효과를 적절히 설명해 내지 못했다. 다른 한편으로는 Tsang(1991), Kavi(1992) 등이 Dolan(1989)의 이론을 응용한 보-데크 연결법(BDC)을 도입하여 해석하였다. 이 BDC모델은 보 요소의 이 보 요소에 강체링크로 연결되어 있는 스프링 요소가 보요소의 각 절점을 공유하게 되므로, 아무리 많은 스프링을 가정하더라도 자유도수는 증가하지 않는다는 것이다. 그러므로 돔 구조와 같은 대형 구조물을 해석할 때 컴퓨터 용량과 계산시간을 절약할 수 있다.

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무한요소(Infinite Elements)를 이용한 기초공학해석

  • 양신추
    • Computational Structural Engineering
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    • v.4 no.2
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    • pp.9-12
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    • 1991
  • 공학문제에 있어서, 해석적으로 접근할 수 없었던 많은 경우의 문제들이 유한요소법(Finite Element Methods)의 정형화된 모형화 및 해석과정을 통하여 쉽게 접근되어질 수 있었다. 최근 보다 효율적인 요소개발과 컴퓨터 기술의 발달로 유한요소법은 더욱 효과적인 해석 수단이 되어가고 있다. 그러나 지반공학 문제와 같은 무한영역 문제를 유한요소법으로 해석할 경우, 매우 큰 영역을 모형화하기 위하여 많은 수의 요소가 요구되며 이에 따른 자유도(Degree of Freedom) 수의 증가로 많은 계산시간을 요구하게 된다. 본 고는 무한영역 문제를 효과적으로 모형화하기 위하여 연구, 개발되어진 무한요소(Infinite Element)에 대하여 소개하려 한다. 무한요소의 기본개념과 강성행렬의 형성방법을 보인 후, 기초공학 문제를 예로 하여 이의 적용방법을 간략하게 설명하였다.

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Free Vibrations and Buckling Loads of Simple Beam-Columns with Constant Volumes (일정체적을 갖는 단순지지 보-기둥의 자유진동 및 좌굴하중)

  • Lee, Byoung-Koo;Oh, Sang-Jin;Mo, Jeong-Man;Lee, Yong
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 1995.04a
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    • pp.62-69
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    • 1995
  • The differential equations governing both the free vibrations and buckling loads of the beam-columns with constant volumes are derived and solved numerically. The axial load effects are included in the differential equations. The Runge-Kutta method and Regula-Falsi method are used to compute the eigenvalues corresponding to the natural frequencies. and buckling loads. In numerical examples, the simple end constraint is considered.

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The Aspects of Accepting Existentialism in the Novels by Chang Yong-hak and Son Chang-sub (장용학과 손창섭 소설에 나타난 실존주의 수용 양상)

  • Eum, yeong-cheol
    • Proceedings of the Korea Contents Association Conference
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    • 2015.05a
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    • pp.405-406
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    • 2015
  • 본 연구는 장용학과 손창섭을 중심으로 고찰하였다. 연구된 바 얻어진 결론을 요약하면 다음과 같다. 장용학의 <요한 시집(詩集)>은 '자유'의 의미가 '성실한 삶'으로 이어지는데 이는 싸르트르의 직접적인 영향이 있었던 것으로 보인다. 손창섭의 <미해결의 장>은 '광순'이를 통해 부조리한 현실을 고발한다는 측면에서 까뮈의 영향이 간접적으로 나타난다고 볼 수 있다.

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