• Title/Summary/Keyword: 자연발병

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Identification and Characterization of Entomopathogenic Fungi Isolated from Bemisia tabaci in Korea (담배가루이(Bemisia tabaci )에서 분리한 곤충병원성진균의 동정 및 병원성 검정)

  • Park, Hyun-Rho;Ryu, Young-Hyun;Yeon, Il-Kyen;Nam, Sung-Hee;Kim, Dong-Geun;Han, Myung-Sae
    • Korean journal of applied entomology
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    • v.53 no.1
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    • pp.27-34
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    • 2014
  • Entomopathogenic fungi were isolated from Bemisia tabaci in an Oriental melon field, and their growth characteristics, factors related to a natural outbreak, and infectivity against Bemisia tabaci, Tetranychus urticae, and Frankliniella intonsa were investigated. The isolates had erect conidiophores bearing whorls of 4-6 phialides with a swollen base where cylindrical conidia of $3.0-3.4{\mu}m$ were attached. The isolates were identified as Isaria fumosorosea on the basis of morphological characteristics and an ITS sequence with 99% similarity. I. fumosorosea IFs-08 grew well on Sabouraud dextrose agar+yeast extract medium(3.2 mm/day/$24^{\circ}C$); it grew better at $35^{\circ}C$ than at $15^{\circ}C$. The isolates of I. fumosorosea-IFs were highly infective and killed 93.9-96.7% B. tabaci, 84.9-92.0% T. urticae, and 81.5-84.4% F. intonsa in bioassay, whereas three isolates (Isaria tenuipes, Isaria farinosa, and Isaria fumosorosea) from KACC showed a low infectivity of 10-20%. To the best of our knowledge, this is the first report of I. fumosorosea isolated from B. tabaci in Korea.

건강과 자연농업-제232호

  • Korea Organic Farming Association
    • THE HEALTH and ORGANIC FARMING
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    • no.232
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    • pp.1-12
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    • 2006
  • 제2 8회 한국유기농업대회장에서/제11회 제주특별자치도 유기농업대회 개최/우리의 산야초-은방울꽃/과수나무 수확직후 토양검정을 하고 밑거름은 땅이 얼기 전에 주어야 좋다/벼 멀칭재배의 이론과 기술/협회 회관 착공식/'유기가공식품인증제' 도입/미국 통상정책의 두얼굴/쌀과 같은 위치서 감귤협상/두가지 비료로 유기농작물의 고품질 다수확 생산을 체계적으로 보다 쉽게 하는 방법/개정 친환경농업육성법 공포/온실작물들의 잿빛곰팡이병 관리/시설 내 병해의 발병 특성과 방제대책/중국 박람회 및 유기농장 연수기/'07년도 인증위원회 개최공고/중국 친환경농업의 평가와 시사점/친환경 유기낙농의 정착 방안/유기농업으로 이룬 쿠바의 낙원/유기질비료의 이해

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건강과 자연농업-제237호

  • Korea Organic Farming Association
    • THE HEALTH and ORGANIC FARMING
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    • no.237
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    • pp.1-12
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    • 2007
  • 3만2천여 회우동지들의 염원이던 '유기농회관' 준공후 입주준비완료/과대포장된 장밋빛 F T A의 허구성/<암을 이기는 음식>(2) 녹황색 채소/미국, 유기농식품시장 매년 두자릿수 성장/애완동물 사료도 유기농 바람, 리콜 이후 크게 늘어/"친환경유기농업 육성정책"의 성공조건/도시 어린이들에게 친환경농업교육 실시/사과 겹무늬썩음병의 발병생태/미생물을 이용한 질병의 방제/작물건전생육에 토양개량이 무엇보다 중요/염류장해 뿌리혹선충 극복/농업선진화운동본부, 친환경농산물 유통개혁세미나/벼 멀칭재배의 이론과 기술/미국에서도,친환경 웰빙쌀이 '바람몰이'/친환경농업 연수교육/우리의 산야초-범부채/탄질률과 영양주기 이론의 이해/유기질비료의 이해

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Biological and Physiochemical structure change of Black pine bast scale (Matsucoccus thunbergianae) in coast area of Korea (해송림에 만연된 솔껍질깍지벌레 피해목의 물리 화학적 및 생물학적 구조변화에 관한 연구(III))

  • 김규진;김종완;임기표
    • Korean journal of applied entomology
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    • v.34 no.3
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    • pp.174-180
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    • 1995
  • The damage rate according to the age of black pine trees in the Black pine bast scale, Matsucoccus thunbergianae was high from 7 to 22 year old trees. According to damaged degree of damaged trees, it showed great difference in infection rate of saprophitic fungi. The period until the developmental degree of saprophitic fungi became maximized degree of 7 was required about 48 days in dead tree of 100 percent, about 54 days in one of above than 80 percent, about 75 days in one of 40~50 percent, but the dead tree less than 10 percent and health tree was retained the 3~4 developmental degree of saprophitic fungi, after 80 days. The ring width of damaged trees showed as follows. The non damaged trees was 3.52 cm, 0.77 cm in the damaged trees of 100 percent, 0.88 cm in the ones of 80 percent, 1.22 cm in the ones of 40~50 percent and 1.37 cm in the ones of les than 10 percent, respectively. Number of cells per unit area was smaller in the case of greater damaged trees, gradually. Also moisture content was very high in greater damaged trees, and bending strength was lower. Extracts by the one percent NaOH showed a small amount.

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Acute Descending Necrotizing Mediastinitis Secondary to Spontaneous Rupture of Hypopharynx -A Case Report- (자연성 인두천공에 의한 급성하행 괴사성 종격동염 - 1례 보고 -)

  • 조규도;박찬범;조덕곤;김치경;왕영필;곽문섭
    • Journal of Chest Surgery
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    • v.35 no.11
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    • pp.842-846
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    • 2002
  • A rare case of acute descending necrotizing mediastinitis(DNM) secondary to spontaneous rupture of hypopharynx is reported. Due to the right lower cervical abscess the patient had undertaken limited right anterior cervical drainage at other department. After transferring to our department he undertook combined wide cervical mediastinal drainage and mediastinal drainage via right thoracotomy. However mediastinal drainage was ineffective. So the second operation was performed and chest tubes were relocated. He had an uneventful postoperative course. As far as the cause of acute descending necrotizing mediastinitis was concerned, we couldn't find any literature in Korean or English except one case that dealt with spontaneous rupture of the hypopharynx.

A case of neonatal peroneal neuropathy with intrauterine onset (신생아 종아리신경병증 1례)

  • Lee, Sang-Soo;Sim, Ji-Yun;Kim, Mi-Jung
    • Clinical and Experimental Pediatrics
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    • v.50 no.6
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    • pp.585-587
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    • 2007
  • Peroneal neuropathy presenting at birth is a rare disorder. Although neonatal mononeuropathies may be related to obstetrical complications, prenatal mechanisms should be also considered. We describe an infant who was born at term by cesarean section due to breech presentation with a unilateral footdrop. Lack of compound muscle action potential in the peroneal nerve and denervation potentials confined to the tibialis anterior and the extensor hallucis longus muscles in the electrophysiological studies on the fourth day of life strongly suggest an isolated peroneal neuropathy of intrauterine onset. Early and sequential electrodiagnostic studies will be important to provide better temporal and pathophysiologic definitions, the better timing of onset and prognosis for mononeuropathies presenting in newborn infants.

Porcine Eperythrozoonosis-like Disease in Korea: Epidemiological Observations (한국에서의 돼지 Eperythrozoon 증양 질병 : 역학적 연구)

  • Rim, Byung-moo;Kim, James C.S.;Han, Du-seok
    • Korean Journal of Veterinary Research
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    • v.24 no.2
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    • pp.237-243
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    • 1984
  • 한국에서 돼지에 감염되고 있는 Eperythrozoon 증양 질병의 역학적 생물학적 기초연구를 위해 본 질병에 자연감염 발병한 돼지 157두와 정상인 돼지 240두에 대하여 조사하였던 바 다음과 같은 결과를 얻었다. 혈액표본에서 관찰한 본 병원체의 감염율 형태 및 크기는 계절에 따라 변화를 보였는데, 정상인 돼지는 년평균 54% 감염율을 발병한 돼지는 모두 감염된 상태였다. 5종의 형태를 보이는 본병원체는 크기가 $0.5{\sim}0.5{\mu}$이었는 데 감염 혈액을 $0^{\circ}C$에 1주 이상(혹은 $-20^{\circ}C$에 24시간) 냉장하는 경우 그 크기가 $1.4{\sim}8.5{\mu}$까지 커졌으며 세포질내에 염색질 과립을 보이는 예도 발견되었다. 본 병원체는 적혈구막에 부착되거나 혈장내 유리된체 관찰되었으며 병든 돼지의 위장관과 방광내용물 중에서도 다수의 병원체가 발견되었다. 인공감염 실험에서 접종 감염된 돼지는 2개월간 경도의 체온상승과 빈혈상을 나타내면서 혈액내에 전형적인 병원체를 보유하였으며 접종시킨 쥐에서는 12개월이상 동일한 병원체의 잠복 감염상태를 유지하였다. 또한 병든 돼지 주위에서 포획한 파리와 모기의 장내용물 중에서 전형적인 본 병원체를 관찰할 수 있었다.

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The Occurrence of Strawberry Black Leaf Spot Caused by Alternaria alternate (Fr.) Keissler in Korea (딸기 검은무늬병(Alternaria alternata (Fr.) Keissler)의 발생에 관하여)

  • Cho J.T.;Moon B.J.
    • Korean journal of applied entomology
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    • v.19 no.4 s.45
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    • pp.221-227
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    • 1980
  • A new disease of strawberry caused by Alternaria was found in Gim Hae, Gyougnam province, in July 1979. The symptoms of the disease occurred mainly on leaves as showing dark brown spots with circular to irregular, $5\~6mm$ in diameter. The pathogen was identified as Alternaria alternate (Fr.) Keissler which has not been described in Korea as a pathogen of strawberry disease. The common name of the disease was given as Gum un mum byung of strawberry. The conidia shape and size of this fungus varied considerably with culture media. The measurements of conidia and beak on corn meal agar were $20.1\times9.3{\mu}\;and\;4.0\mu$, respectively, which were shorter than those on strawberry leaf lesions in the field. Among 49 strawberry varieties tested, Morioca 16 and Robinson were highly susceptible to the disease by artificial inoculation and Catskill, Cyclone, Northwest, Merton princess, Juspa and Daehak I were moderately susceptible whereas 31 varieties were resistant. This fungus could also infect the leaf of Common garden sunflower, Double sunflower and Mexican sunflower among the 14 plants species tested.

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Effects of Tomato-Juice and Potassium Phosphate on the Infection of Botryis cinerea LVF12 on the Tomato Leaves (토마토쥬스와 KH$_2$PO$_4$가 Botrytis Cinerea LVF12 분생포자의 토마토 감염에 미치는 영향)

  • 손지희;이재필;김철승;임은경;송주희;김현주;박현철;문병주
    • Research in Plant Disease
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    • v.7 no.3
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    • pp.134-139
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    • 2001
  • Effects of tomato-juice and KH$_2$PO$_4$ as exogenous nutrients on the infection of Botrytis cinerea LVF12 and pathogenicity to tomato were investigated. B. cinerea LVF12, which was previously reported as a casual agent of the gray mold rot of perilla, was used for pathogenesis on tomato leaves. No infection was induced, and no lesion developed on tomato leaves by the conidial suspension of LVF12 when the inoculum was prepared in sterilized water. However, when the conidial suspensions of LVF12 added with various concentrations and conditions of tomato-juice were inoculated on whole tomato plants, the disease was induced readily, Among them, 20% tomato juice with 0.1M KH$_2$PO$_4$ appeared to be the most suitable nutrient to promote high disease incidence on tomato. For the pathogenicity test according to the growing stage of tomato, the mature leaves were more susceptible than seedlings. Symptoms on the infected plants were initial small gray spots at the inoculated area. Later the whole leaves, petioles and stems became gray and eventually fell off, Under high humidity conditions, the diseased leaves and stems were covered with gray hyphae and conidia. All symptoms of infected plants were identical to those in the field conditions.

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Resistance to the Fungal Pathogen Phytophthora infestans of Transgenic Potato Plants Harboring of Chitinase Gene (Chitinase 유전자 도입 형질전환 감자식물체의 역병저항성)

  • Choi, Kyung-Hwa;Yang, Duk-Chun;Kim, Hyun-Soon;Choi, Kyung-Ja;Cho, Kwang-Yeon;Jung, Hyuk
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.3
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    • pp.177-182
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    • 1999
  • A fungal infection assay between normal and transgenic potato harboring chitinase gene in cultivar Belchip was investigated. In the first stage of experiment, seven transgenic lines having 12cm tall were tested for their resistance against potato late blight pathogen Phytophthora infestans by infection with the zoospores, artificially, Susceptibility to potato late blight infection could be classified into three types based on the rate. In terms of resistance to the disease, two lines were higher, two lines were more suppressive, and three lines were similar as compared with the control. In the following experiment, only 2 risistant lines and 1 suppressed line were used to confirm the resistance again. The results of both experiments were similar. Furthermore, two highly resistant transgenic lines grown in field exhibited a higher resistance than control under the conditions of natural ocurrence of the fungal disease.

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