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http://dx.doi.org/10.5656/KSAE.2013.09.1.081

Identification and Characterization of Entomopathogenic Fungi Isolated from Bemisia tabaci in Korea  

Park, Hyun-Rho (Gyeongbuk Agricultural Research & Extension Services)
Ryu, Young-Hyun (Gyeongbuk Agricultural Research & Extension Services)
Yeon, Il-Kyen (Gyeongbuk Agricultural Research & Extension Services)
Nam, Sung-Hee (Applied Entomology Division, National Academy of Agricultural Science, RDA)
Kim, Dong-Geun (Gyeongbuk Agricultural Research & Extension Services)
Han, Myung-Sae (Department of Natural Fiber Science, Kyungpook National University Daegu)
Publication Information
Korean journal of applied entomology / v.53, no.1, 2014 , pp. 27-34 More about this Journal
Abstract
Entomopathogenic fungi were isolated from Bemisia tabaci in an Oriental melon field, and their growth characteristics, factors related to a natural outbreak, and infectivity against Bemisia tabaci, Tetranychus urticae, and Frankliniella intonsa were investigated. The isolates had erect conidiophores bearing whorls of 4-6 phialides with a swollen base where cylindrical conidia of $3.0-3.4{\mu}m$ were attached. The isolates were identified as Isaria fumosorosea on the basis of morphological characteristics and an ITS sequence with 99% similarity. I. fumosorosea IFs-08 grew well on Sabouraud dextrose agar+yeast extract medium(3.2 mm/day/$24^{\circ}C$); it grew better at $35^{\circ}C$ than at $15^{\circ}C$. The isolates of I. fumosorosea-IFs were highly infective and killed 93.9-96.7% B. tabaci, 84.9-92.0% T. urticae, and 81.5-84.4% F. intonsa in bioassay, whereas three isolates (Isaria tenuipes, Isaria farinosa, and Isaria fumosorosea) from KACC showed a low infectivity of 10-20%. To the best of our knowledge, this is the first report of I. fumosorosea isolated from B. tabaci in Korea.
Keywords
Entomopathogenic fungi; Bemisia tabaci; Isaria fumosorosea; Natural outbreak; Bioassay;
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